Anthracnose of sweet pepper caused by Colletotrichum scovillei in Japan

Severe fruit rot of sweet pepper was found in Shimane, Hyogo, Chiba, Toyama, and Nagano prefectures, Japan from 2005 to 2011. Dark, sunken spots with concentric rings of orange conidial masses appeared on fruits. Pathogenic isolates from diseased fruits in the prefectures were identified as Colletotrichum scovillei. This species was added to the pathogens of sweet pepper anthracnose in Japan. The representative isolate was pathogenic to sweet pepper, tomato and chili pepper fruits, kidney bean pod, azuki bean, pea and strawberry leaves, but a caused no symptoms on cucumber or carrot in inoculation tests.     

Toxoplasma gondii antibody prevalence and isolation in free-ranging cats in Okinawa,Japan

Cats are an important host of Toxoplasma gondii from an epidemiological perspective because they are the only definitive hosts that excrete oocysts in their feces. In this study, 201 free-ranging cats in Okinawa were examined for T. gondii infection. Using the latex agglutination test, we detected antibodies against T. gondii in 26.9% (54/201) of the cats. Oocysts of T. gondii were not detected upon microscopic examination of the feces of 128 cats. T. gondii was isolated from the tissues of 9 out of 24 seropositive or pseudo-seropositive cats with a bioassay using laboratory mice. Genotyping for the GRA6 gene revealed that five and four of the isolates were type I and II, respectively.     

Novel SNP in 5' flanking region of EDG1 associated with marbling in Japanese Black beef cattle

Marbling, defined by the amount and distribution of intramuscular fat, is an economically important trait of beef cattle in Japan. The endothelial differentiation , sphingolipid G-protein-coupled receptor , 1 ( EDG1 ) gene has been considered as a positional functional candidate for the gene responsible for marbling. We have recently reported that 2 single nucleotide polymorphisms (SNPs), c.-312A > G in the 5' untranslated region (UTR) and c.*446G > A in the 3' UTR in EDG1 were associated with marbling in Japanese Black beef cattle, but this was not functional and a causal mutation for marbling. In the present study, we detected 2 novel SNPs, referred to as g.1475435G > A and g.1471620G > T , in the 5' flanking region of the EDG1 between low-marbled and high-marbled steer groups, which were previously shown to have EDG1 expression differences in musculus longissimus muscle. The g.1475435G > A SNP seemed not to segregate in Japanese Black beef cattle. The g.1471620G > T SNP was associated with the predicted breeding value for beef marbling standard number by the analyses using Japanese Black beef cattle population. Based on these findings, we hypothesized that the g.1471620G > T SNP might have an impact on EDG1 expression and also marbling.     

Identification of a potent immunostimulatory oligodeoxynucleotide from Streptococcus thermophilus lacZ

Immunostimulatory sequences of oligodeoxynucleotides (ODNs), such as CpG ODNs, are potent stimulators of innate immunity. Here, we identified a strong immunostimulatory CpG ODN, which we named MsST, from the lac Z gene of Streptococcus (S.) thermophilus ATCC19258, and we evaluated its immune functions. In in vitro studies, MsST had a similar ability as the murine prototype CpG ODN 1555 to induce inflammatory cytokine production and cell proliferation. In mouse splenocytes, MsST increased the number of CD80+CD11c+and CD86+CD11c+ dendritic cells and CD4+CD25+ regulatory T cells. We also analyzed the effects of MsST on the expression of regulatory cytokines by real-time quantitative PCR. MsST was more potent at inducing interleukin-10 expression than the ODN control 1612, indicating that MsST can augment the regulatory T cell response via Toll-like receptor 9, which plays an important role in suppressing T helper type 2 responses. These results suggest that S. thermophilus , whose genes include a strong Immunostimulatory sequence-ODN, is a good candidate for a starter culture to develop new physiologically functional foods and feeds.     

Emphysematous cystitis in a chemically-induced diabetic dog

Emphysematous cystitis is a rare disorder caused by bacterial infection and characterized by gas accumulation within the bladder wall with cyst formation. This report describes the histopathological characteristics of emphysematous cystitis found in a diabetic female beagle induced by streptozotocin and alloxan. Macroscopically, multiple cyst-like structures were observed on the cut surface of the urinary mucosa. During fixation, small specimens cut from the mucosa floated on the surface of the fixative solution. Histopathologically, multiple cysts were lined with a single layer of flattened cells found to be immunohistochemically positive for vimentin, partially positive for α-smooth muscle actin or macrophage scavenger receptor, class A, and thought to be myofibroblasts, fibroblasts or macrophages. Multinucleated giant cells were observed around the cysts, and gram-negative short bacilli were observed in the lumen of the urinary bladder. From these findings, this case was diagnosed as emphysematous cystitis.     

Elevated atmospheric CO2 concentration alters the effect of phosphate supply on growth of Japanese red pine (Pinus densiflora) seedlings

We demonstrated that the inorganic phosphate (P(i)) requirement for growth of Japanese red pine (Pinus densiflora Sieb. & Zucc.) seedlings is increased by elevated CO(2) concentration ([CO(2)]) and that responses of the ectomycorrhizal fungus Pisolithus tinctorius (Pers.) Coker & Couch to P(i) supply are also altered. To investigate the growth response of non-mycorrhizal seedlings to P(i) supply in elevated [CO(2)], non-mycorrhizal seedlings were grown for 73 days in ambient or elevated [CO(2)] (350 or 700 micromol mol(-1)) with nutrient solutions containing one of seven phosphate concentrations (0, 0.02, 0.04, 0.06, 0.08, 0.10 and 0.20 mM). In ambient [CO(2)], the growth response to P(i) was saturated at about 0.1 mM P(i), whereas in elevated [CO(2)], the growth response to P(i) supply did not saturate, even at the highest P(i) supply (0.2 mM), indicating that the P(i) requirement is higher in elevated [CO(2)] than in ambient [CO(2)]. The increased requirement was due mainly to an altered shoot growth response to P(i) supply. The enhanced P(i) requirement in elevated [CO(2)] was not associated with a change in photosynthetic response to P(i) or a change in leaf phosphorus (P) status. We investigated the effect of P(i) supply (0.04, 0.08 and 0.20 mM) on the ectomycorrhizal fungus P. tinctorius in mycorrhizal seedlings grown in ambient or elevated [CO(2)]. Root ergosterol concentration (an indicator of fungal biomass) decreased with increasing P(i) supply in ambient [CO(2)], but the decrease was far less in elevated [CO(2)]. In ambient [CO(2)] the ratio of extramatrical mycelium to root biomass decreased with increasing P(i) supply but did not change in elevated [CO(2)]. We conclude that, because elevated [CO(2)] increased the P(i) requirement for shoot growth, the significance of the ectomycorrhizal association was also increased in elevated [CO(2)].     

阻燃处理木材燃烧残余物的热分解特征

为了弄清楚阻燃处理木材燃烧残余物的热分解特征,将阻燃处理木材在模拟的典型火灾中燃烧后,取距燃烧表面不同位置的试样,采用热失重法研究了阻燃处理木材燃烧残余物的热分解过程,结果表明:①阻燃处理木材及其燃烧残余物的热分解开始温度没有明显的差别,未处理木材燃烧残余物的热分解开始温度比未燃烧木材高;②阻燃处理木材中阻燃剂的热分解峰值温度为200℃,随着燃烧过程的进行,归属于阻燃剂的峰消失;③阻燃处理木材燃烧残余物热分解温度曲线中,在230℃附近归属于半纤维素的峰消失,在210～240℃出现了一个缓慢的肩;④阻燃处理木材及其燃烧残余物的质量损失速度曲线主峰温度比未处理木材及其燃烧残余物降低100℃,质量损失速度大幅度减少;⑤阻燃处理木材在600℃时的热分解残存质量比未处理木材显著增大,随着燃烧时受热温度的增高,燃烧残余物热分解的残余质量显著增大;⑥阻燃处理木材及其燃烧残余物的热分解温度区间,与未处理木材及其燃烧残余物存在显著差异.      

Strategies and engineering adaptions to disseminate SRI methods in large-scale irrigation systems in Eastern Indonesia

The system of rice intensification (SRI) developed in Madagascar has been controversial in part because there have been no large-scale, long-term evaluations of the impact of its alternative methods. This paper summarizes experience with the dissemination of SRI practices across eight provinces in Eastern Indonesia over nine seasons from 2002 to 2006 under a major irrigation project. The Decentralized Irrigation System Improvement Project (DISIMP) was financed by the Japanese Government with project management by a Nippon Koei consultant team. SRI has been introduced in Indonesia via several organizations and in different parts of the country starting in 2000. The evaluation reported here, made by the DISIMP technical assistance team, is based on data from 12,133 on-farm comparison trials that covered a total area of 9,429 ha. Under SRI management, average paddy yield increase was 3.3 t/ha (78%). This was achieved with about 40% reduction in water use, 50% reduction in chemical fertilizer applications, and 20% lower costs of production. The farmers whom DISIMP was assisting to take up SRI were usually cultivating their paddy fields individually within irrigation systems where it was difficult to reduce water applications as recommended for SRI. Accordingly, innovations had to be made in soil and water management to create relatively aerobic soil conditions so that farmers could get the more productive rice phenotypes expected from SRI practice. This article describes the modifications made to adapt SRI concepts, pointing to the value of introducing in-field ditches, which was confirmed through paddy tract surveys. This experience and analysis showed how SRI methods could be utilized within irrigation systems where water management was not (yet) tailored to SRI production practices. Subsequently, modifications in irrigation system management are being made to be more supportive of SRI cultivation.