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621.
Lynn WR 《Science (New York, N.Y.)》1977,195(4274):150-155
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Berrettini W Bierut L Crowley TJ Cubells JF Frascella J Gelernter J Hewitt JK Kreek MJ Lachman H Leppert M Li MD Madden P Miner C Pollock JD Pomerleau O Rice JP Rutter JL Shurtleff D Swan GE Tischfield JA Tsuang M Uhl GR Vanyukov M Volkow ND Wanke K 《Science (New York, N.Y.)》2004,304(5676):1445-7; author reply 1445-7
623.
We demonstrated in laboratory experiments that temperature control of marine bacteria action on diatoms strongly influences the coupling of biogenic silica and organic carbon preservation. Low temperature intensified the selective regeneration of organic matter by marine bacteria as the silicon:carbon preservation ratio gradually increased from approximately 1 at 33 degrees C to approximately 6 at -1.8 degrees C. Temperature control of bacteria-mediated selective preservation of silicon versus carbon should help to interpret and model the variable coupling of silicon and carbon sinking fluxes and the spatial patterns of opal accumulation in oceanic systems with different temperature regimes. 相似文献
624.
Human immunodeficiency virus type-1 (HIV-1) membrane fusion is promoted by the formation of a trimer-of-hairpins structure that brings the amino- and carboxyl-terminal regions of the gp41 envelope glycoprotein ectodomain into close proximity. Peptides derived from the carboxyl-terminal region (called C-peptides) potently inhibit HIV-1 entry by binding to the gp41 amino-terminal region. To test the converse of this inhibitory strategy, we designed a small protein, denoted 5-Helix, that binds the C-peptide region of gp41. The 5-Helix protein displays potent (nanomolar) inhibitory activity against diverse HIV-1 variants and may serve as the basis for a new class of antiviral agents. The inhibitory activity of 5-Helix also suggests a strategy for generating an HIV-1 neutralizing antibody response that targets the carboxyl-terminal region of the gp41 ectodomain. 相似文献
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克隆了拟南芥热激因子AtHSFa1a,并由热激启动子AtHSP70b控制,构建了双元表达载体pV-70bAtHSFa1a,并经农杆菌介导转化烟草,获得了转基因植株,并检测了其耐热性指标.结果显示:与对照相比,转基因烟草的电导率较低,丙二醛含量增幅较小,SOD酶活性较高,初步表明其对高温具有更高的耐受性. 相似文献
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Gilbert MT Tomsho LP Rendulic S Packard M Drautz DI Sher A Tikhonov A Dalén L Kuznetsova T Kosintsev P Campos PF Higham T Collins MJ Wilson AS Shidlovskiy F Buigues B Ericson PG Germonpré M Götherström A Iacumin P Nikolaev V Nowak-Kemp M Willerslev E Knight JR Irzyk GP Perbost CS Fredrikson KM Harkins TT Sheridan S Miller W Schuster SC 《Science (New York, N.Y.)》2007,317(5846):1927-1930
Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections. 相似文献