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41.
Schönherr J Fernández V Schreiber L 《Journal of agricultural and food chemistry》2005,53(11):4484-4492
Time courses of cuticular penetration of FeCl3 and Fe(III) complexes of citric acid, EDTA, EDDHA (Sequestrene 138Fe), imidodisuccinic acid (IDHA), and ligninsulfonic acid (Natrel) were studied using astomatous cuticular membranes (CMs) isolated from Populus x canescens leaves. At 100% relative humidity, the Fe(III) chelates disappeared exponentially with time from the surface of the CMs; that is, penetration was a first-order process that can be described using rate constants or half-times of penetration (t(1/2)). Half-times ranged from 20 to 30 h. At 90% humidity, penetration rates were insignificant with the exception of Natrel, for which t(1/2) amounted to 58 h. Rate constants were independent of temperature (15, 25, and 35 degrees C). Permeability decreased with increasing Fe chelate concentration (IDHA and EDTA). At 100% humidity, half-times measured with FeIDHA were 11 h (2 mmol L(-1)), 17 h (10 mmol L(-1)) and 36 h (20 mmol L(-1)), respectively. In the presence of FeEDTA, penetration of CaCl2 was slowed greatly. Half-times for penetration of CaCl2, which were 1.9 h in the absence of FeEDTA, rose to 3.12 h in the presence of an equimolar concentration of EDTA and 13.3 h when the FeEDTA concentration was doubled. Hence, Fe chelates reduced permeability of CMs to CaCl2 and to the Fe chelates themselves. It is suggested that Fe chelates reduced the size of aqueous pores. This view is supported by the fact that rate constants for calcium salts were about 5 times higher than for Fe chelates with the same molecular weights. Adding Tween 20 (5 g L(-1)) as a humectant did not increase permeability to FeIDHA at 90% humidity and below, while addition of glycine betaine did. Penetration of FeCl3 applied at 5 g L(-1) (pH 1.5) was not a first order process as rate constants decreased rapidly with time. Only 2% of the dose penetrated during the first 2 h and less than that in the subsequent 8 h. Recovery was only 70%. This was attributed to the formation of insoluble Fe hydroxide precipitates on CMs. These results explain why in the past foliar application of Fe compounds had limited success. Inorganic Fe salts are instable and phytotoxic because of low pH, while Fe chelates penetrate slowly and 100% humidity is required for significant penetration rates. Concentrations as low as reasonably possible should be used. These physical facts are expected to apply to stomatous leaf surfaces as well, but absolute rates probably depend on leaf age and plant species. High humidity in stagnant air layers may favor penetration rates across stomatous leaf surfaces when humidity in bulk air is below 100%. 相似文献
42.
Effects of diethylsuberate (DESU), tributyl phosphate (TBP), and monodisperse ethoxylated alcohols (EAs) on rate constants of penetration (k) of model solutes across astomatous cuticular membranes isolated from Madagascar ivy (Stephanotis floribunda) and pear (Pyrus communis) leaves were studied. Model solutes (selected on the basis of their octanol/water partition coefficients, K(ow)) were iprovalicarb (log K(ow) = 3.18), metribuzin (log K(ow) = 1.60), and methyl glucose (MG) (log K(ow) = -3.0). K(ow) varied by more than 6 orders of magnitude. Accelerators had wax/water partition coefficients (log K(ww)) ranging from 1.75 (DESU) to 4.32 (C(12)E(2)), and their equilibrium concentrations in Stephanotis wax varied from 0 to about 160 g kg(-)(1). Accelerators increase solute mobility in cuticles by increasing fluidity of cutin and waxes. This effect was quantified by plotting log k versus the accelerator concentration in wax. With the lipophilic solutes metribuzin and iprovalicarb, these plots were linear. Slopes of these plots characterize the intrinsic activities of the accelerators, and they decreased in the order DESU (0.029) > TBP (0.015) > EAs (0.01). Using these intrinsic activities, the effects of accelerators on rate constants of penetration can be calculated for any accelerator concentration in wax. For instance, at 50 g kg(-)(1), rate constants for lipophilic solutes increased by factors of 28 (DESU), 5.6 (TBP), and 3.2 (C(12)E(n)()), respectively. Permeability of cuticles for the hydrophilic MG was not increased by DESU, TBP, C(12)E(2), and C(12)E(4), while C(12)E(6) and C(12)E(8) increased it. Small hydrophilic solutes such as MG can access aqueous pores in cuticles, and this pathway is not affected by changes in fluidity of amorphous waxes. After rate constants of penetration of ionic CaCl(2) were compared with those for nonionic MG, it was concluded that 60% of the MG diffused across aqueous pores, while 40% used an alternative pathway. Because the solubility of MG in wax is extremely low, it is unlikely that MG diffused along the lipophilic pathway used by metribuzin and iprovalicarb. This agrees with the observation that DESU and TBP had no effect on rate constants for MG. An alternative pathway of unknown properties is suggested. It is speculated that C(12)E(6) and C(12)E(8) sorbed in cuticles might have generated a polar pathway for MG. 相似文献
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46.
Liu Sen Li Peng Van Zwieten Lukas Tu Jia Gan Weixiang Lu Sheng Wang Hailong Wu Lichao 《Journal of Soils and Sediments》2021,21(8):2922-2937
Journal of Soils and Sediments - Paulownia is the fastest-growing timber species all over the world which depended on exogenous nutrient input and scattered in the subtropical region of China.... 相似文献
47.
Lukas P. Stoltz 《African Zoology》2013,48(1):367-378
Several baboon troops were studied during a two year study period. Sub-troop formation in troops was regularly observed and such sub-troops are defined as temporary or semi-permanent. Sub-troops are either normally or abnormally composed as far as age and sex classes are concerned. A temporary sub-troop sleeps apart from the main sub-troop, and these sub-troops reunite the following morning. A semi-permanent sub-troop separates from the main sub-troop for several days. During this period the semi-permanent sub-troop only joins the main sub-troop at sleeping places. The behaviour of certain males is prominent when sub-troops are formed. This study revealed that the baboon troop is wrongly referred to as a stable unit. 相似文献
48.
Deoxynivalenol oligoglycosides: new "masked" fusarium toxins occurring in malt, beer, and breadstuff
M Zachariasova M Vaclavikova O Lacina L Vaclavik J Hajslova 《Journal of agricultural and food chemistry》2012,60(36):9280-9291
The co-occurrence of deoxynivalenol-3-glucoside with its parent toxin, deoxynivalenol, has been recently documented in many cereal-based foods, especially in those produced by enzyme-catalyzed processes. The presence of this masked mycotoxin in the human diet has become an issue of health concern, mainly because of its assumed bioavailability. A selective immunoaffinity-based preconcentration strategy, followed by ultrahigh-performance liquid chromatography coupled with high-resolution orbitrap mass spectrometry, revealed that, in addition to the most common deoxynivalenol-3-glucoside, also oligoglycosylated deoxynivalenols with up to four bound hexose units were present in cereal-based products. The structure, origination, and fate of these deoxynivalenol conjugates during malt/beer production and bread baking have been thoroughly investigated. Special attention has been paid to the changes of deoxynivalenol conjugates enabled by industrial glycosidase-based enzymatic preparations. To the authors' best knowledge, this is the first study documenting the complexity of masked deoxynivalenol issue. 相似文献
49.
Vesely Tomas Neuberg Marek Trakal Lukas Szakova Jiřina Tlustoa Pavel 《Water, air, and soil pollution》2012,223(4):1847-1859
The effects of one of the most toxic heavy metals, lead (Pb), applied in two different concentrations and combined with chelate
application were investigated on the water macrophyte (Pistia stratiotes L.) physiology. The influences were observed by the chlorophyll and free amino acid content determination. Also the lead
accumulation in macrophyte biomass was investigated to assess the potential efficiency of this plant for rhizofiltration of
highly Pb-polluted water. Na EDTA and Na citrate were used as chelates and Pb(NO3)2 as lead supplement. The application of organic chelates simulated conditions of an induced phytoextraction process. Statistical
analyses were performed as a one-way ANOVA with a subsequent Tukey HSD test at a level of P < 0.05. Pb contents in both root and leaf tissues gradually increased with increasing Pb concentrations in the nutrient solution.
More lead was accumulated in leaves than in roots within all treatments. The total chlorophyll content decreased with increased
Pb concentration and with a higher content of chelates. The chelate addition increased the total amino acid content in leaves
but decreased the total amino acid content in roots. The addition of lead with chelates decreased the dry biomass weight.
However, water macrophyte showed extremely high lead accumulation in biomass in the short term (up to 8 days) and this accumulation
potential could be used for relatively fast and effective decrease of high concentration of this risk element in contaminated
water or sewage. 相似文献
50.
Oldrich Navratil Olivier Evrard Michel Esteves Sophie Ayrault Irène Lefèvre Cédric Legout Jean-Louis Reyss Nicolas Gratiot Julien Nemery Nicolle Mathys Alain Poirel Philippe Bonté 《Journal of Soils and Sediments》2012,12(9):1463-1478