Rapid detection of canine parvovirus in feces using monoclonal antibodies and enzyme-linked immunosorbent assay

Monoclonal antibodies were used to develop a double antibody enzyme-linked immunosorbent assay for the detection of canine parvovirus (CPV) antigen in fecal samples. The assay was specific for the hemagglutinating protein of CPV and detected as little as 1.5 ng of virus within a 15-minute incubation period. The use of monoclonal antibodies against 2 epitopes on the CPV antigen permitted the simultaneous addition of test sample and enzyme-conjugated antibody, thus considerably simplifying the manipulations required for the assay. Results were visually determined without special instrumentation. Clinical studies revealed greater than 95% correlation between enzyme-linked immunosorbent assay results and hemagglutination titers.     

The use of digital image analysis and real‐time PCR fine‐tunes bioassays for quantification of Cercospora leaf spot disease in sugar beet breeding

Cercospora leaf spot, caused by the fungus Cercospora beticola, is a major fungal sugar beet disease worldwide and the cause of significant yield losses. The disease is most successfully countered by the introduction of genetic tolerance into elite sugar beet hybrids. To this end, breeding programmes require high quality biological assays allowing discrimination of minor differences between plants within a segregating population. This study describes the successful implementation of image analysis software in the bioassays for quantification of necrotic lesions at different stages of C. beticola infection, allowing selection on minor phenotypic differences during the sugar beet breeding process for C. beticola resistance. In addition, a real‐time PCR assay was developed for the quantification of C. beticola pathogen biomass in infected beet canopy. The use of both techniques, even in an early stage of infection, fine‐tunes current bioassays, allowing more accurate and efficient selection of resistant breeding material.     

Stochastic efficiency analysis of deficit irrigation with standard risk aversion

The main objective of this research was to develop an expected utility optimisation model to economically evaluate deficit irrigation within a multi-crop setting while taking into account the increasing production risk of deficit irrigation. The dynamic problem of optimising water use between multiple crops within a whole-farm setting when intraseasonal water supply may be limited was approximated by the inclusion of multiple irrigation schedules into the optimisation model. The SAPWAT model (South African Plant WATer) was further developed to quantify crop yield variability of deficit irrigation while taking the non-uniformity of irrigation applications into account. Stochastic budgeting procedures were used to generate appropriately correlated matrixes of gross margins necessary to incorporate risk into the water use optimisation model. Special care was taken to represent risk aversion consistently between the alternatives through the use of a new procedure to standardise values of absolute risk aversion. The model was applied to study the impact of increasing levels of risk aversion on the profitability of deficit irrigation under limited water supply conditions. The main conclusion from the analyses was that although deficit irrigation was stochastically more efficient than full irrigation under limited water supply conditions, irrigation farmers would not willingly choose to conserve water through deficit irrigation and would be expected to be compensated to do so. Deficit irrigation would not save water if the water that was saved through deficit irrigation were used to plant larger areas to increase the overall profitability of the strategy. Standard risk aversion was used to explain the simultaneous increasing and decreasing relationship between the utility weighted premiums and increasing levels of absolute risk aversion and was shown to be more consistent than when constant absolute risk aversion was assumed.     

Effects of accelerators on mobility of 14C-2,4-dichlorophenoxy butyric acid in plant cuticles depends on type and concentration of accelerator

Effects of diethyl suberate (DESU), diethyl sebacate (DES), dibutyl suberate (DBSU), dibutyl sebacate (DBS), and tributyl phosphate (TBP) on diffusion of 14C-2,4-dichlorophenoxy butyric acid (2,4-DB) across cuticular membranes (CM) was studied. Astomatous CM were isolated enzymatically from Stephanotis floribunda Brongn. leaves, and diffusion was measured at 20 degrees C. The alkyl-substituted dicarboxylic acids constitute a homologous series with carbon numbers increasing from C12 to C18. Molecular weights increased only moderately from 230.0 (DESU) to 314.5 (DBS), while partition coefficients varied over orders of magnitude from 92 (DESU), to 1213 (DES), to 15,988 (DBSU), to 210,762 (DBS). All the above compounds turned out to be accelerators as they increased 2,4-DB mobility by up to 40-fold with accelerator concentrations in the CM ranging from only 9.2 to 105 g kg(-1). Efficacy (2,4-DB mobility in the presence/mobility in the absence of accelerators) increased with increasing concentrations of accelerators in CM or in reconstituted cuticular waxes. Plotting efficacy vs accelerator concentration in the CM resulted in straight lines, and their slopes increased in the order DBS (0.14), DBSU (0.31), DES (0.51), and DESU (0.85). Hence, DESU was the most powerful accelerator in this series as it increased 2,4-DB mobility in the CM about 6 times more than DBSU. Waxes constitute the major barrier in plant cuticles, and plots of efficacy vs accelerator concentration in Stephanotis wax were also linear, but compared to CM slopes were steeper by factors of 3.20 (DBS), 2.97 (DBSU), 2.70 (DES), and 1.62 (DESU). TBP was similarly effective as DESU, but plots of efficacy vs concentration were not linear, and curves approached a plateau at 60-80 g kg(-1). These data are discussed with regard to suitability of these accelerators for formulating systemic pesticides.     

Rural Governance, Forestry, and the Promotion of Local Knowledge: The Case of the German Rural Development Program ‘Active Regions’

This article investigates the role of local knowledge in the policy approach of neo-endogenous rural development, which may be held by local and regional actors such as municipal and county level politicians, local and regional level administration, entrepreneurs as well as local third sector representatives. The main question addressed is whether local knowledge under this approach is merely mobilised or if rural development processes are even based on such knowledge, placing it at the centre of such policy. The relevance of this approach to forestry is also explored. The types of forest owners for which the neo-endogenous rural development approach is appropriate and the types that may have difficulties in coping with this cross-sectoral and area-based approach are especially examined. Using a case study design on the German Active Regions funding program it is shown that the neo-endogenous approach is most relevant to large private forest owners and small-scale corporate enterprises, but also has potential for small- and medium-sized private owners. Concerning the role of knowledge held by local and regional actors, the approach is shown to largely build on the use of local expertise, and hence policy acceptance increases. However, scientific knowledge as well as politics are found to play a major role in the use, production and interpretation of local knowledge.     

The contribution of Asian dust in the pedogenesis of ultisols in Southeastern China determined by soil grain size

Journal of Soils and Sediments - The present study was conducted to understand the pedogenesis of soils developed on basalts and reveal the impact of Asian dust on soils in subtropical China. Soils...     

A review of carbon isotopes of phytoliths: implications for phytolith-occluded carbon sources

Journal of Soils and Sediments - Phytolith-occluded carbon (PhytOC) is mainly derived from the products of photosynthesis, which can be preserved in soils and sediments for hundreds-to-thousands of...     

The Role of Biological Efficacy Evaluation and Methodology in Registration

For both governmental agencies and industry, considerable advantages, especially from extrapolation of data within a country or between countries, can be derived from co-ordinated action in the efficacy testing of new compounds. This has been recognized at an early stage in Europe, i.e. by France (1952) and the Federal Republic of Germany, where methods have been devised in a common agreement between official services and pesticide manufacturers. The European Weed Research Council started work for international alignment on herbicide testing, whereas EPPO, in 1971, initiated collaborative studies on rodents¹⁾ (5 methods), fungi (5 methods), insects and mites (10 methods). These guide-lines, having been approved by the 35 EPPO Governments, are already published or will be published in the near future; a further 29 methods are presently under study. Since 197, contacts have been taken with the American Society for Testing and Materials in order to reach inter-regional agreement on testing methods.