Ovine anaplasmosis in Northwest China

Tropical Animal Health and Production - This paper described studies on the distribution, vector and multiplication of the pathogen of ovine anaplasmosis and a complement fixation test (CFT) for...     

商丘大豆种质及其利用

对商丘大豆种质的历史演变历程、种质体系的建立、种质特性及利用进行综合剖析，为合理利用商丘大豆种质提供参考依据。     

Establishment of proliferative cell nuclear antigen gene as an internal reference gene for polymerase chain reaction of a wide range of archival and fresh mammalian tissues.

Polymerase chain reaction (PCR) from paraffin-embedded tissues provides a powerful tool to amplify DNA from a variety of recent and archival material. Because DNA from paraffin-embedded samples is more degraded than from fresh material, the amplification of reference genes is essential to exclude false-negative results. This study describes the use of the proliferative cell nuclear antigen (PCNA) gene as a reference gene in a range of animal species and in humans. The PCNA-PCR to amplify a fragment extending from exon 5 through exon 6 and including the intervening intron 6 gave a reproducible pattern, with a 280-base pair (bp) band from canine, equine, bovine, ovine, and caprine samples showing high sequence homology. Porcine, guinea pig, tiger, and lion samples, however, gave an additional fragment of approximately 197 bp. The whole intron 6 from these fragments is missing, possibly representing a pseudogene. In feline samples only the 197-bp fragment could be detected. This study shows that the PCNA gene is highly conserved across a broad range of animal species and is well suited as an internal control for PCR analysis in veterinary medicine.     

磷酸二铵作水稻基肥的效果

在水稻上进行不同基肥施用对比试验 ,结果表明 :分别用磷酸二铵 15 0、2 2 5kg/hm2 ,与习惯用碳铵 3 75kg/hm2 +普钙 3 75kg/hm2 作基肥的水稻相比 ,产量分别增加 169.5和 3 15kg/hm2 ,增产 2 .3 %和 4.4% ,达显著水平。用磷酸二铵代替碳铵和普钙作水稻基肥 ,具有省力、增产等优点。     

浅析质量手册的作用及质量管理体系的局限性

质量手册用来对组织的质量管理体系进行充分阐述,规定质量管理体系的基本结构,并指导组织实施体系的主要文件。因此,组织的质量手册就成为建立并运行质量管理体系的重要标志。1体现了组织对质量管理体系的总体策划的思路组织在对其质量管理体系进行总结策划时,必须高瞻远瞩,从组织的整体利益和长远发展角度出发,对质量管理体系进行全面的统筹规划。质量手册中的所有规定正是这种全面规划思路的体现。组织围绕产品或服务质量形成全过程的各个阶段,考虑应开展的过程控制和质量活动,对这些过程和活动的控制要求进行系统设计和描绘,并将相应的责…     

由体外受精囊胚获得ICR小鼠ES细胞

收集鼠卵母细胞和精子，采用体外受精技术，获得早期胚胎。体外培养胚胎至囊胚，分离内细胞团，获得ICR小鼠的ES细胞。结果不但获得了来自体外受精囊胚的ICR小鼠ES细胞，而且表明用体外受精方法获得的小鼠早期胚胎的数量明显大于常规方法，且比较稳定。ICR小鼠ES细胞的获得，为研究小鼠的ES细胞分化提供了条件。     

家蚕血液、消化液中酸性核糖核酸酶和天冬酰胺酶活性的初步研究

测定了家蚕夏芳、长灰A和大造各品种血液、消化液中酸性核糖核酸酶及家蚕秋白、夏芳、长灰A和大造各品种天冬酰胺酶性变化。结果表明：各品种血液、消化液中酸性核糖核酸酶、天冬酰胺酶活性变化随发育呈现较强的规律性，血液中，4龄第3d、5龄第3d这两种酶均出现峰值，4龄眠前活性较低，消化液中，5龄第3d这两种酶活性均较高，不同品种这两种酶活性峰值出现时间有差异；各品种血液中酸性核糖核酸酶、天冬酰胺酶性均明显高于相应品种消化液中这两种酶活性；各品种之间血液、消化液中这两种酶活性有明显差异。     

Effect of testosterone on atherosclerosis in rabbits

AIM: To investigate the action of testoster one on atherosclerosis in a rabbit model. METHODS: 37 male cholesterol-fed rabbits were divided into five groups: castration group: castrated rabbits without exogenous testosterone admin istration; testosterone Ⅰ group: castrated rabbits with exogenous testosterone 0.25 mg·kg-1·d-1; testosterone Ⅱ group: castrated rabbits with e xogenous testosterone 2.5 mg·kg-1·d-1; testosterone Ⅲ group: cas trated rabbits with exogenous testosterone 12.5 mg·kg-1·d-1. The sham operation group was also set. Three months later, the levels of testosteron e, blood lipids (including TG, HDL-C, LDL-C), PAI activity, nitric oxide (NO) co ntent, endothelin (ET), angiotensin Ⅱ (AngⅡ) in blood were detected. RESULTS: It showed that testosterone in castration group was the lowest. There was no significant difference of TG or LDL-C between castration g roup and the other four groups. HDL-C in castration group was lower than that in other four groups. NO content of castration group was lower than that in others , but PAI activity, ET and AngⅡ concentration were higher than that in other gr oups. CONCLUSION:Testosterone is a protective factor against atherosc lerosis in male rabbits.     

东北亚四国（地区）SMV株系毒力比较

将我国，韩国，日本及俄罗斯远东地区的ＳＭＶ株系与鉴别寄主交互接种测定其毒力。结果指出采用外国的株系鉴别寄坟难以区分我国株系毒力。同样，用我国鉴别寄坟也难以区分外国毒株的毒力，所测定的我国９个株系的１３个代表毒株全部能侵染最抗病的鉴别寄主韩国的Ｂｕｆｆａｌｏ和日本的Ｈａｒｏｊｏｙ。     

输气管道缺陷尺寸定量化及其概率分布特性研究

通过对四川某输气管道现场采样管段的室内缺陷探测，建立了以现场采样，无损检测、切检测拉断和缺陷尺寸测试为主要过程的缺陷探测和尺寸定量化方法。对采样管段探测结果表明，管子内焊耻存在严重的表面裂纹，裂纹平均深度为１．９１ｍｍ，最大深度为３．８５ｍｍ，采用了正态分布，对数正态和威布尔分布拟合缺陷尺寸的概率分布规律，结果表明裂纹深度α最接近正态分布。