树莓试管苗茎尖包埋玻璃化法超低温保存

摘要：本试验对树莓茎尖包埋玻璃化法种质资源保存进行了研究,以期建立简单、高效的树莓种质资源超低温保存体系。试验以树莓试管苗茎尖为材料,探讨了不同因素对包埋玻璃化法超低温保存后成活率的影响。结果表明：长约1mm的树莓试管苗茎尖用海藻酸钙包埋后,采用分步预培养的方法,最后终止蔗糖浓度为0.9mol/L的MS培养基中进行预培养3d,再用含2M甘油和0.9M蔗糖的装载液处理90min, 在0℃下用PVS2处理180min后投入液氮中保存1h,在40℃下化冻3min,用含1M蔗糖的MS液体培养基洗涤20min,最后转到恢复培养基上,30d后在没有形成愈伤组织的情况下形成新的植株。以上的保存程序应用于6个树莓品种,成活率达87%。该结果为树莓种质资源的长期保存提供了理论依据。     

泰州市郊区土壤与作物施肥信息系统研究与开发

运用农业信息技术原理与方法,结合泰州市郊区实际,开发出了功能全面、内容丰富、现势性强、图形生产力高、界面友好、易于操作的基于Visual Basic 6.0和MapObjects 2.3的泰州市郊区土壤与作物施肥信息系统V1.0。该系统涵盖了数据查询检索、空间分析、精确施肥决策、学习辅助等功能。经试用,系统的功能可用性、功能可靠性、易用性、可扩充性较强,用户满意度较高,可为该地推广应用土壤管理与水稻、小麦精确施肥技术提供辅助决策与智能学习优质平台。     

质膜内在蛋白ZmPIP1;1参与玉米耐旱性和光合作用的功能分析

质膜内在蛋白(plasma membrane intrinsic proteins, PIPs)是水通道蛋白主要亚家族成员之一,在植物生长发育过程中具有重要调节功能。前期研究表明,玉米ZmPIP1;1基因表达受到渗透和盐胁迫的强烈诱导,但其在玉米中的生物学功能尚不明确。本研究通过玉米遗传转化获得了ZmPIP1;1超表达转基因株系,干旱胁迫实验揭示了ZmPIP1;1超表达转基因株系较野生型具有较低的水分散失率及较强的干旱胁迫耐性。转录组测序结果表明参与ABA生物合成及其信号通路相关基因的表达水平发生了显著变化。在田间正常生长条件下,ZmPIP1;1超表达转基因植株与野生型在生长发育过程中没有明显差异,但转基因玉米株系具有较高的光合效率,粒宽和百粒重增加,玉米单果穗的产量提高。此外,通过荧光双分子互补实验观察到ZmPIP1;1和ZmPIP2;6蛋白在玉米叶肉细胞原生质体的细胞质膜和叶绿体膜上存在互作,并且可能导致了ZmPIP蛋白的重定位。该研究为ZmPIP1;1分子机制的解析奠定了重要基础,为玉米高光效分子设计育种开辟了新的途径。     

Affine invariant shape recognition with particle swarmoptimization algorithm

Focusing on the problem that affine transformation will exist among the contour images due to variation of the viewpoints, a new approach to extract affine invariant features and matching strategy is proposed for shape recognition. First, the centroid distance and azimuth angle of each boundary point are computed. Then, with a prior defined angle interval, all the points in the neighbor region of the sample point are considered to calculate the average distance for eliminating noise. After that, the centroid distance ratios(CDRs) of any two contour points with angle difference of 180° are achieved as the representation of the shape, which would be invariant to affine transformation. Since the angles of contour points changed non linearly among affine related images, the CDRs should be resampled to build corresponding relationship. It could be regarded as an optimization problem of path planning. In our method, a PSO based path planning model is presented to address this problem. The experimental results demonstrate the efficiency of the proposed method in shape recognition with translation, scaling, rotation, distortion and noise interference.     

Cd2+对番茄幼苗生长和蛋白质组的影响

以3 d龄番茄幼苗为试验材料, 从生理、生化和蛋白质组角度, 分析0.01~1.00 mmol L^–1 Cd²⁺处理72 h后对幼苗的影响。结果表明, Cd²⁺处理导致幼苗生长严重受抑, 幼苗高度从对照组的4.76±0.50 cm分别降至3.79±0.05 cm (0.01 mmol L^–1 Cd²⁺处理, P<0.01)和1.77±0.15 cm (0.03 mmol L^–1 Cd²⁺处理, P<0.01)。根长度从对照组的6.07±0.04 cm降至4.77±0.58 cm (0.01 mmol L^–1 Cd²⁺处理, P< 0.01)和3.65±0.66 cm (0.03 mmol L^–1 Cd²⁺处理, P<0.01)。叶绿素含量在0.1 mmol L^–1 Cd²⁺处理后开始下降。当幼苗用0.05 mmol L^–1 Cd²⁺处理时, 根系中有10个蛋白质斑点, 叶片中有21个蛋白质斑点产生变化。利用MS/MS技术, 根系中有4个蛋白质斑点得以鉴别, 它们是ribosomal protein L 20 (斑点1)、F-box /LRR repeat protein (斑点2)、ribosomal protein small submit 4 (斑点4)和CBL-interacting protein kinase (斑点5)。在叶片中, 有2个蛋白质斑点消失, 4个蛋白质斑点合成, 它们是ABC transporter (斑点16)、maturase-like protein (斑点17)、chalcone synthase (斑点1)、a hypothetical protein (斑点3)、an unknown protein (斑点4)和a predicated protein (斑点6)。这些被鉴别的Cd²⁺反应蛋白参与生物合成、mRNA转录调控和蛋白质转运。     

种苗处理对麦冬生长的影响及其控病效果研究

为了明确种苗处理对麦冬生长的影响及其控病效果,指导麦冬优质安全生产.以川麦冬为供试材料,采用田间小区试验,研究了 6种药剂3种种苗处理方式对麦冬种苗成活、生长和产量的影响,以及对炭疽病、根腐病的控病效果.结果表明木霉T23制剂、多抗霉素和噁霉灵处理种苗,可显著提高麦冬种苗成活率,促进麦冬分蘖、增加块根数、使块根伸长增粗...     

Effects of fruit thinning on ascorbate–glutathione cycle metabolism in black currants (<Emphasis Type="Italic">Ribes nigrum</Emphasis> L.)

To elucidate mechanisms regulating ascorbic acid (AsA) biosynthesis and accumulation in the fruit and leaves of black currants, AsA and the activities of key enzymes in the ascorbate–glutathione (AsA–GSH) cycle were measured from fruit set to fruit ripening during fruit thinning treatments of three common commercial black currant cultivars that differed in their AsA levels: ‘Risager’ (low), ‘Brodtrop’ (medium) and ‘Adelinia’ (high). Treatments were 50% fruit reduction (50% of total fruit set) by hand, control was no thinning. Fruit thinning treatment significantly increased AsA content in fruit of all three cultivars from weeks 2 to 8, significantly decreased AsA content in leaves from weeks 3 to 8. Dehydroascorbate reductase and monodehydroascorbate reductase activities in fruit and leaves had a similar pattern, increasing during week 2, rose until they peaked in week 4. Ascorbate peroxidase activity in fruit in the thinning treatment was slightly lower than in the control. Fruit thinning was shown to be a good model to test AsA biosynthesis regulation and accumulation in black currants. The results from our study provided strong evidence that AsA–GSH cycle involved in AsA synthesis and accumulation in fruit.     

日本系统收获表的研究现状及前景

文中阐述了系统收获表的由来,解析了其基本构成和功能,介绍了目前日本编制的系统收获表的种类和具体的编制方法,并以信州地区落叶松系统收获表为例简单说明其输入、输出的方式和图表格式,最后分析了系统收获表在森林资源管理中的应用前景.     

老鹰茶白粉病罹病植株的生理生化研究

供试的不同老鹰茶品种被病原菌侵染后，叶片中叶绿索含量、光合速率和蒸腾速率均随着感病程度的加剧而明显地下降，感病品种下降幅度大于抗性品种；而丙二醛含量随着感病程度的加剧而明显上升，感病品种增加的幅度比抗性品种大。老鹰茶叶片中叶绿素含量与其光合速率有显著的正相关关系。