Antiproliferative activity of Citrus juices and HPLC evaluation of their flavonoid composition

The antiproliferative activity of fresh fruit juices extracted from Citrus sinensis (cv. Washington Navel and cv. Sanguinello), C. deliciosa cv. Avana, C. clementina cv. Nules, C. aurantium subsp. myrtifolia , was evaluated against K562 (human chronic myelogenous leukemia), HL-60 (human leukemia) and MCF-7 (human breast adenocarcinoma) cell lines. All the juices tested showed antiproliferative activity. Moreover, the pattern of the main flavanone compounds in the juices has been determined by HPLC analysis.     

Decomposition of woody legume nodules in two tree/grass associations under contrasting environmental conditions

Turnover of nitrogen-rich root nodules follows the pruning of legume trees, forming a potentially important yet little studied way of N release to the soil. The effects of soil moisture, herbivory by soil mesofauna and microbial decomposition on the disappearance rate of woody legume nodules was studied in two tree/grass forage production associations. Litter bags containing nodules of Erythrina variegata L. (Papilionoideae: Phaseoleae) were incubated for four weeks in grass-covered alleys between Gliricidia sepium (Jacq.) Walp. (Papilionoideae: Robinieae) hedgerows, established on a deep alluvial Oxisol under a humid tropical climate and on a shallow Vertisol under a subhumid tropical climate in Guadeloupe, French Antilles. Soil moisture was regulated by irrigating or covering small plots from natural rainfall. Fine nylon mesh bags were used to study the rate of microbial decomposition, and open-ended perforated cylinders were used to estimate nodule herbivory. The chemical traits, especially the lignin: nitrogen ratio, of E. variegata and G. sepium nodules were similar (lignin: N 1.70 and 1.55, respectively), and suggest that the results are probably also applicable to the G. sepium nodules in the associations. Both soil moisture and decomposing agent (microbes or mesofauna) had a significant effect on the nodule disappearance rate, but soil type did not have any apparent effect. The nodule half-life varied from three to seven days under different treatments. The N release rate from the nodules was high, with N half-life varying from three to five days. Herbivory accounted for ca. 10% of total mass and N loss from nodules during the four-week field incubation period, but its importance increased towards the end of the incubation, especially in Vertisol, after the most easily decomposable part of the nodules had decayed. After pruning, the nodule N is released to soil more rapidly than from mulch. This revised version was published online in June 2006 with corrections to the Cover Date.     

Influence of season on the hematological and serum biochemical values of bottlenose dolphins (Tursiops truncatus) housed in a controlled environment in northern Italy

Season appears to influence the physiology of bottlenose dolphins (Tursiops truncatus). From 2000 to 2005, blood parameters of three captive bottlenose dolphins housed in northern Italy were examined to determine if seasonal variation was present. Seasonal variation was observed in the male dolphins, with both males exhibiting aminotransferase levels that were higher in autumn and lower in winter. Mean serum creatinine levels were higher during summer and lower during autumn in the adult male, and mean lactate dehydrogenase higher during summer and lower during spring in the juvenile male. Both males exhibited red blood cell counts and hemoglobin levels that were higher during autumn and lower during summer. This study contributes to the knowledge of baseline hematologic and biochemical values based on seasonality in bottlenose dolphins.     

Use of culture-derived Fusarium oxysporum f. sp. cubense, race 1 filtrates for rapid and non-destructive in vitro differentiation between resistant and susceptible clones of field-grown banana

Banana and plantain are among the most important food crops in developing countries but production is threatened by increasing virulent forms of Fusarium oxysporum f. sp. cubense. Chemical control is not economically effective and,therefore, breeding programs are necessary. Traditional field studies of new genotype resistance to this disease are time-consuming and destructive. Therefore,we developed a rapid and non-destructive procedure to differentiate field-grown banana resistant from susceptible clones. This procedure implicates application of culture filtrates of Fusarium oxysporum f. sp. cubense race 1 onto banana leaves. The relationship between duration of the fungal in vitro incubation, and the fungal culture fresh mass, the culture filtrate absorbency, and the Gross Michel (susceptible cultivar)leaf lesion area (after application of the culture filtrate) were similar and at 24day-incubation the highest values of the recorded indicators were observed. A comparison between Gross Michel and FHIA-01(resistant) was also performed. The most relevant differences between cultivars were observed at 48 hours after application of the culture filtrate, and in the middle-aged leaves. The position of the culture filtrate application in the leaf limb (distal, middle, proximal) was not determinant. A wider comparison among banana cultivars confirmed previous results informed by other researchers using different systems to study this plant-fungus interaction. Such a confirmation validates the effectiveness of the procedure described here to select rapid and non-destructively banana resistance to this disease at field level. This revised version was published online in August 2006 with corrections to the Cover Date.     

Phenol metabolism of two cultivars of durum wheat (Triticum durum Desf.) as affected by ozone and flooding stress

To compare the phenolic responses under oxidative stressors, plants of two Italian cultivars of durum wheat (Claudio and Mongibello) were (a) exposed to ozone (O₃) (80 ppb, 5 hr/day for 70 consecutive days), with the aim to investigate the changes of phenolic compound contents in their leaves, or (b) flooded (seven consecutive days). Plants showed O₃-induced visible injury, but their photosynthetic performance was not affected by the pollutant. Specifically, Claudio showed a higher O₃ tolerance than Mongibello. The major value of the present study is undoubtedly the pioneering investigation of phenolic metabolism of durum wheat under O₃. We identified 12 foliar phenolic compounds in all leaf samples (i.e. controls, exposed to O₃ and flooded): ten phenolic acids, a flavanol (catechin hydrate) and a phenolic aldehyde (syringaldehyde). Overall, O₃ exposure resulted in accumulations of phenolic compounds, especially in Claudio. These responses can be likely considered a fine-regulated repair process that equipped Claudio stressed plants with an antioxidant system capable of scavenging oxidative stress. Different phenolic variations were found in flooded plants, suggesting that phenolic response to environmental constraints is stress specific. Our study confirms that investigations and characterization of specific phenolic profiles of crop cultivars under oxidative stress may be helpful in breeding programmes.     

Physiology, diagnosis, and diseases of the avian reproductive tract.

Disorders of the reproductive system represent a large portion of both large and small domestic animal medicine. Although some disorders of this system have been extensively studied in birds, this science is still in its infancy, when compared to mammalian reproductive medicine. This may be due to several reasons, but the simple fact that birds are oviparous renders knowledge of mammalian reproductive anatomy, histology, physiology, and disease process, inapplicable to avian patients. Nevertheless, several specific diseases or conditions affecting the reproductive system of birds have been described. By integrating information about the comparative anatomy and physiology of birds, reports of the most common reproductive diseases of birds and utilizing the latest diagnostic techniques, especially videoendoscopy, the avian practitioner should be able to diagnose and properly treat a high percentage of the avian reproductive diseases.     

Cytosine arabinoside in addition to VCAA‐based protocols for the treatment of canine lymphoma with bone marrow involvement: does it make the difference?

Cytosine arabinoside (ara‐C) is a component of many protocols for the treatment of acute leukaemia and non‐Hodgkin lymphomas in humans. The aim of the study was to prospectively evaluate the efficacy of ara‐C in a myeloablative regimen in a cohort of canine lymphomas with bone marrow involvement. Seventeen dogs were enrolled. Eight were treated with a VCAA‐based protocol (Group 1) and nine with the same regimen added with ara‐C (Group 2). Ara‐C was administered on a 5‐day schedule as an i.v. continuous infusion at the dose of 150 mg m^?2 per day for five consecutive days. During treatment complete remission (CR) was achieved in two dogs in Group 1 and in eight dogs in Group 2. CR rate was significantly higher in Group 2 (P < 0.01). Median survival was 72.5 days (range 6–174) in Group 1 and 243 days (range 73–635) in Group 2. Survival was significantly longer in Group 2 (P < 0.001). Both protocols were well tolerated, with a low incidence of adverse events. Ara‐C added to a VCAA‐based protocol appears to be safe and beneficial in dogs with stage V lymphoma. Incorporation of the nucleoside analogue might be crucial for the development of future therapeutic strategies in dogs.