中小型饲料企业的客户服务体系建设

我们推销的是什么，可以吸引客户的是什么?有人说是产品，有人说是形象，还有人说是企业的经营理念，是企业的文化，但是无论怎样都不能忽视“服务”。对中小型饲料企业来说．由于没有能力建立一个庞大而独立的服务体系．因此需要部门之间紧密合作，分工不分家．把销售部门、生产部门、采购部门、办公室和计财部门整合为一体，     

钙蛋白酶系统在动物营养中的应用

钙蛋白酶(Calpain)系统包括钙蛋白酶、钙蛋白酶抑制蛋白(Calpastatin)和钙蛋白酶激活蛋白(CalpainActivator)。研究表明,钙蛋白酶系统通过参与肌原纤维蛋白的降解过程从而影响肌肉生长及肉的嫩化。胞内Calpain活性受多种因素的调节,包括钙需要量、自溶裂解、磷脂的作用、钙蛋白酶抑制蛋白和激活蛋白等,然而对于体内钙蛋白酶的活性如何调控的争议仍然存在。     

Identification of polymorphisms and association analysis with meat quality traits in the porcine KIAA1717 and HUMMLC2B genes

Skeletal muscle genes are potential candidates for production and meat quality. Screening a subtracted cDNA library constructed with mRNA obtained from longissimus dorsi muscles of F1 hybrids Landrace × Yorkshire and their female parents Yorkshire, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. Database search revealed KIAA1717 and HUMMLC2B encoded proteins with SET domain and EF-hand calcium binding motif, respectively. In the present work we identified their partial polymorphisms and two SNPs, one (C1354T) at the 3′ untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, both created/disrupted a restriction site for endonuclease Msp I. The selected pigs were genotyped at the KIAA1717 C1354T and HUMMLC2B A345G sites by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with meat marbling (longissimus doris (p < 0.05), biceps femoris (p < 0.01)) and intramuscular fat (p < 0.01). HUMMLC2B A345G were significantly associated with meat pH (longissimus doris (p < 0.05), biceps femoris (p < 0.01)), drip loss (p < 0.01), water holding capacity (p < 0.01) and meat color value (longissimus doris (p < 0.01), biceps femoris (p < 0.05)). Further studies are needed to confirm these preliminary results.     

不同的干燥工艺对荷花蜂花粉功能成分的影响

探讨了5种干燥工艺对荷花蜂花粉功能成分的影响。微波干燥的干燥效率最高,60%火力处理540s可使水分从31.41%降到6.42%;干燥方式对荷花蜂花粉蛋白质影响较小;微波干燥后总黄酮提取率最高,为13.20mg/g;真空冷冻干燥后维生素C和过氧化氢酶含量最高,分别为48.89mg/100g和29.86mg/g·min。总的来说,真空冷冻干燥对荷花蜂花粉的功能成分影响最小;常压恒温干燥对功能成分影响较小、工艺简单、对设备要求低,宜于大规模应用于实际生产中。     

一例猪附红细胞体病的诊治

附红细胞体病是以溶血性贫血、黄疸、发热为主要特征的人畜共患的血液感染性疾病,其病原体为附红细胞体。本病例通过流行病学调查、临床症状观察、尸体剖检和实验室检验,确诊为猪附红细胞体病。在采取综合性防治措施后疫情得到了有效控制。     

预防奶牛难产的几项基本措施

难产可使胎儿和母牛的死亡率升高,导致产后母牛繁殖力和生产性能降低,给养牛业造成很大的经济损失。奶牛难产可分为母源性和胎儿性难产,主要取决于产力、产道、胎儿三个因素以及他们的相互关系。其中,胎儿过大是影响难产发生率的最重要的原因,母牛初孕年龄、公母牛品种、胎次等     

Pyruvate kinase is necessary for Brucella abortus full virulence in BALB/c mouse

Brucellosis, caused by a facultative intracellular pathogen Brucella, is one of the most prevalent zoonosis worldwide. Host infection relies on several uncanonical virulence factors. A recent research hotpot is the links between carbon metabolism and bacterial virulence. In this study, we found that a carbon metabolism-related pyruvate kinase (Pyk) encoded by pyk gene (locus tag BAB_RS24320) was associated with Brucella virulence. Determination of bacterial growth curves and resistance to environmental stress factors showed that Pyk plays an important role in B. abortus growth, especially under the conditions of nutrition deprivation, and resistance to oxidative stress. Additionally, cell infection assay showed that Pyk is necessary for B. abortus survival and evading fusion with lysosomes within RAW264.7 cells. Moreover, animal experiments exhibited that the Pyk deletion significantly reduced B. abortus virulence in a mouse infection model. Our results elucidated the role of the Pyk in B. abortus virulence and provided information for further investigation of Brucella virulence associated carbon metabolism.     

对动物防疫法中牵连违法行为的适用分析

1牵连违法情形在动物防疫法的存在动物卫生执法是《中华人民共和国动物防疫法》实现的重要途径和有力保障,严格遵守《中华人民共和国     

Development of a disperse dye immunochromatographic test for the detection of antibodies against infectious bursal disease virus

For investigating the feasibility of using disperse dyes as an immunoassay chromogenic marker, a disperse dye, DADISPERSE NAVY BLUE SP, was selected in analyzing antibody against infectious bursal disease virus (anti-IBDV). With the color intensity revealed in the disperse dye immunochromatographic test (DICT) strip as the objective function, the optimal dyeing conditions were found as follows: dye concentration absorbance (at lambda(max)=587nm)=3, pH 7, 50 degrees C, for 10min. Under these conditions, the resultant dyed-antibody (rabbit anti-chicken) can produce an optimal color intensity reading of 55,054 on the strip. For performing qualitative immunoassay, chicken sera samples taken from different farms were used for the anti-IBDV titre assessment. The results of DICT strips showed very high sensitivity and specificity as compared to that analyzed by FlockChek enzyme linked immunosorbent assay (F-ELISA) kits. For quantitative immunoassay, it was found that the color intensity measured with DICT was linearly correlated to that of F-ELISA titre (r(2)=0.9687). Therefore, DICT was further applied to the detection of chicken anti-IBDV sera under vaccination in the farms. The average titres of the sampling groups exhibited a strong agreement to that of F-ELISA. Accordingly, the DICT method developed in this study, shown to be reliable, cheap and simple in both qualitative and quantitative immunoassays, is particularly suitable for point-of-need testing (PONT) in agricultural applications.