基于知识仓库的虚拟产品开发技术探析

基于知识仓库的虚拟产品开发技术整合社会资源,利用web技术在不实际生产产品实物的情况下,利用模块化思想采用网络化并行设计,在产品设计初期就考虑到后续工艺和加工需求以及用户可能的附加要求,可缩短新产品开发周期。本文基于知识仓库,针对虚拟产品开发技术,分析虚拟产品技术的国内外研究现状,阐述虚拟产品开发技术的原理以及相关技术,构造虚拟产品开发的设计流程,并且基于Delphi8.0网络环境下构建基于知识的虚拟产品创新设计制造个人工作空间子系统。     

砖混结构墙体裂缝的探讨

本文针对砖混结构墙体出现裂缝的问题,通过分析其原固,提出了消除裂缝的途经及措施。     

黑龙江省农业企业内部绩效评价

随着农业产业化的推进,农业企业得到较快发展,为了实现农业企业的科学管理,要求对农业企业内部绩效进行评价。网络区间DEA方法是将农业企业分为若干个非完全独立的子系统,每个子系统中包括输入、输出和中间变量。笔者在大量理论研究的基础上,采用网络DEA方法和区间DEA方法相结合,建立农业企业绩效评价的模型,并利用DPS数据处理软件进行求解,然后对黑龙江省农业企业的内部绩效进行研究,进而找出农业企业内部绩效的影响因素,对其进行深入分析,最后提出农业企业内部绩效改进的方法策略。农业企业每个部门经营的绩效高,减少了农业企业各部门不必要资源的投入,提升了农业企业的内部绩效水平。     

The use of T‐DNA tagging to isolate mutants of Colletotrichum gloeosporioides and Colletotrichum acutatum with reduced virulence against Hevea brasiliensis

Agrobacterium tumefaciens‐mediated transformation (ATMT) is being increasingly recognized as an effective insertional mutagenesis tool in studies of filamentous fungi for gene discovery and functional analysis. We developed and optimized ATMT for 2 Colletotrichum species, Colletotrichum gloeosporioides and Colletotrichum acutatum, the causative agents of Colletotrichum leaf disease in rubber trees in Southern China. A. tumefaciens strain AGL‐1 carrying an ILV1 gene and a green fluorescent protein gene were used to transform the conidia of these 2 Colletotrichum species. The transformation efficiency was correlated with the co‐cultivation duration and bacterial cell concentrations, which reached 300–400 transformants per 1 × 10⁶ conidia after optimization. Southern blot analysis indicated that about 60.0% of the C. gloeosporioides transformants and 46.2% of the C. acutatum transformants had a single copy of T‐DNA in their genomes. Fungal genomic DNA segments flanking the T‐DNA were identified in the transformants through thermal asymmetrical interlaced polymerase chain reaction followed by sequencing. The flanking sequences from 4 C. acutatum and 7 C. gloeosporioides transformants showed moderate or weak homology to the NCBI database entries. Some sequences matching those reported virulence‐related genes. The results suggest that the T‐DNA inserted mutants banks constructed are useful for the discovery of new or important genes and to elucidate their function in the 2 Colletotrichum species.     

水库鲢鳙鱼中华鳋病、出血病并发症的诊治技术

信阳市平桥区中小型水库82座,养鱼面积3万多亩。近年来,随着养殖水平的不断提高,中小型水库精养半精养技术得到普遍推广,由于放养密度增大,养殖环境受到污染,每年都出现大批死鱼现象,给养殖户带来了极大的经济损失。笔者多年来对中小型水库的疫情进行监控,死鱼的主要原因是中华     

Transport and adsorption of antibiotics by marine sediments in a dynamic environment

Background, aim, and scope  Bed sediments are the major sink for many contaminants in aquatic environments. With increasing knowledge of and research on the environmental occurrence of antibiotics, there has been growing interest in their behaviour and fate in aquatic environments. However, there is little information about the behaviour of antibiotics in a dynamic water/sediment environment, such as river and coastal marine water. Therefore, the aims of the present study were: (1) to study the transport and distribution of four common antibiotics between water and sediment in both dynamic and quiescent water/sediment systems and (2) to understand the persistence and possible degradation of the four antibiotics in the two different systems. Materials and methods  A lid-driven elongated annular flume, designed to reduce the centrifugal effect, was used to simulate a dynamic water environment. In addition, a quiescent water/sediment experiment was conducted for comparison with the dynamic water system. The seawater and sediment, used in both experiments of flowing and quiescent water/sediment systems, were collected from Victoria Harbour, a dynamic coastal environment in an urban setting. The four antibiotics selected in this study were ofloxacin (OFL), roxithromycin (RTM), erythromycin (ETM), and sulfamethoxazole (SMZ), the most commonly used antibiotics in South China. Results and discussion  Antibiotics in an overlying solution decreased very quickly in the flume system due to the sorption to suspended particles and surface sediment. There were significant differences in the adsorption of the four antibiotics in sediment. OFL showed a high tendency to be adsorbed by sediment with a high K _d value (2980 L/Kg), while the low K _d values of SMZ indicated that there was a large quantity in water. The four antibiotics reached a depth of 20–30 mm in the sediment over a period of 60 days in the flume system. However, the compounds were only found in surface sediment (above 10 mm) in the quiescent system, indicating the influence of the dynamic flume system on the distribution of antibiotics in sediment. OFL showed a moderate persistence in the dynamic flume system, while other three antibiotics had less persistence in sediment. However, all of the four compounds showed moderate persistence in the quiescent system. Recommendations and perspectives  The study showed the rapid diffusive transfer of antibiotics from water to sediment in the dynamic flume system. The four antibiotics exhibited larger differences in their adsorption to sediment in both dynamic and quiescent systems due to their different K _d values. The high sorption of antibiotics to marine sediment may reduce their availability to benthic invertebrates.     

Comparison of the florfenicol metabolic rate in Portunus trituberculatus individuals with different body weights

We analysed the metabolic rate of large individuals (LIs) and small individuals (SIs) (based on body weight) of 80‐day‐old specimens of Portunus trituberculatus injected with 40 mg/kg florfenicol (FLR). The results showed that FLR contents in the gills and hepatopancreas were higher in the SIs than in the LIs. The activities of erythromycin N‐demethylase and 7‐ethoxyresorufin‐O‐deethylase related to cytochrome oxidase P450 (CYP) were induced after FLR injection and showed a size‐dependent pattern; however, no significant difference in aminopyrine N‐demethylase activity was found between the LIs and SIs. The activity of the phase II enzyme glutathione S‐transferase (GST) increased in the hepatopancreas after FLR injection, and it was higher in the SIs than the LIs. Moreover, induction of CYP2, CYP3, GST, ABCB and ABCG was observed in the first 24 hr after FLR injection. FLR caused more biomolecular damage in the SIs than the LIs, displaying an obvious body weight‐ and time‐dependent pattern. In summary, the SIs exhibited faster FLR uptake and slower depuration in this species, and FLR caused more biomolecular damage to the SIs.     

Cloning and Protein Structure Analysis of Hypoxia Inducible Factor-1α Gene in Yak

In this study,hypoxia inducible factor-1α(HIF-1α) gene was cloned from yak spleen by RT-PCR,and it was divided into two sections for amplification.The sequence and protein structure were analyzed using related bioinformatics tools.The length of the coding region was 2 472 bp,which encoded 823 amino acids.Its molecule mass was 92.13 ku,and PI was 5.09.It displayed as a hydrophilic protein,whose secondary structure was mainly composed of random coil and α-helix.It had 69 phosphorylation sites and 4 N-glycosylation sites.Phylogenetic tree displayed that Maiwa yak,Bos grunniens,Bos mutus and Bos taurus gathered into one cluster.Maiwa yak HIF-1α gene was successfully cloned in this study,it provided a viable reference for further study of genetic characteristics of HIF-1α.     

GIS在福建龙眼低温冻害分析中的应用

 为减少福建龙眼在低温冻害中的损失,实现科学合理的种植布局,进行本研究。根据龙眼的生长情况、受害程度与年度极端最低气温、种植区域的坡向、坡度的关系,确定龙眼冻害等级指标。利用福建68个气象台站的地理信息资料,建立龙眼冻害指标的空间分布特征模型,通过GIS技术推算出50m×50m分辨率的冻害指标空间分布。利用GIS的空间分析能力,得出福建龙眼低温冻害的分布规律。     

土壤重金属污染及植物修复技术

土壤重金属污染是当今面一临的一个重要环境问题,而土壤重金属污染的植物修复是治理污染土壤的重要手段之一.作者概括了中国土壤重金属污染现状及危害,论述了植物修复技术的类型及其优缺点,并展望了植物修复未来的发展趋势.