开阳县田坝渔业小区实施大鲵养殖的技术措施

为摸索名优水产品养殖经验,加快我县水产养殖结构调整,促进我县渔业健康发展,2007年,我县申报实施了《贵阳市社会主义新农村基础设施建设——养殖基地基础设施建设——渔业小区建设》项目,在花梨乡翁昭村田坝组模拟大鲵生态生活习性新建了100口养殖池的大鲵小区,修建了养殖房、管理房、配套饵料鱼池、饵料鱼暂养池、引水管道等基础设施和配套设施,并于2008年3月25日起引种入场养殖。现将1年来小区开展大鲵人工养殖的技术措施报告如下。     

聚L-乳酸改性麦草共混物材料的研究

以聚L-乳酸(PLLA)和麦草(WS)为原料,以N-甲基吗啉-N-氧化物(NMMO)为溶剂,采用溶液共混法制备了PLLA/WS共混物。首先将麦草溶解在NMMO后,再添加聚L-乳酸并溶解,制备聚L-乳酸与麦草的共混溶液。共混溶液采用浇膜法制备PLLA/WS共混物,采用差示扫描量热法、X射线衍射法、傅里叶变换红外光谱、热重分析和扫描电镜等方法对所得的共混物进行表征。结果表明,聚L-乳酸与麦草可以形成均匀的共混溶液。共混物中PLLA与麦草组分之间具有较强的相互作用,相容性较好。当共混物中PLLA的质量分数为50%时,共混物可以形成结晶结构,且随着PLLA含量的增加,其结晶更加完美,熔点与热稳定性提高。共混物薄膜断面结构较为致密,这说明PLLA与麦草相容性较好,混合均匀。通过调节聚L-乳酸和麦草的配比,可以制备不同性能的生物高分子材料。     

Expression of miRNA-22 in ovarian cancer and effect of miRNA-22 over-expression on SKOV-3 ovarian cancer cell proliferation,migration and invasion

AIM: To examine the expression of miRNA-22 in the ovarian tissues and the effect of miRNA-22 over-expression on the proliferation, migration and invasion in SKOV-3 cells. METHODS: The expression levels of miRNA-22 in different ovarian tissues and SKOV-3 cells were determined by qPCR. miRNA-22 was over-expressed by transfection of miRNA-22 mimic. The cell viability was examined by CCK-8 assay. The cell migration was measured by wound healing test. The cell invasion was analyzed by Transwell assay. The protein expression levels of VEGF and P53 were determined by Western blot. RESULTS: Compared with the normal ovarian tissue, the expression level of miRNA-22 was remarkably decreased in the ovarian tumor tissues. After transfection with miRNA-22 mimic, the expression level of miRNA-22 in the SKOV-3 cells was significantly increased, while the cell viability, migration and invasion were obviously decreased. Moreover, the protein expression of VEGF and P53 was dramatically inhibited after over-expression of miRNA-22. CONCLUSION: The decreased miRNA-22 expression may be correlated with the development of ovarian can-cer. Over-expression of miRNA-22 decreases the cell viability, migration and invasion by reducing the protein expression of VEGF and P53.     

辽东栎伐桩萌苗的发育规律

通过对黄土高原黄龙山林区辽东栎林固定样地连续6年的调查,研究间伐强度分别为13.4％(弱度间伐)和30.0％(强度间伐)的辽东栎伐桩萌苗的存活与生长规律.结果表明:1)间伐第1年,辽东栎伐桩均有萌生枝发生,每桩萌生枝数量和萌生枝高度在不同强度间无显著差异;2)萌生枝数量受伐桩高度和直径的影响,当伐桩高度较低时,萌生枝数量随伐桩高度的增加而增加,超过20cm后随之减少,呈三次抛物线关系;伐桩萌生枝数量随着伐桩直径增加呈现先增加后减少的趋势,直径30～50cm的伐桩萌生枝数量最多;3)伐桩萌生枝数量与最长萌生枝高度呈显著线性负相关,存在“萌生枝数量——高度权衡”现象;4)间伐第6年,伐桩的存活率为97.01％,强度间伐区萌生枝高度显著高于弱度间伐区;5)调查期间,辽东栎伐桩萌生枝数量逐年下降,伐桩产生的萌生枝数量越多,后期萌生枝死亡的数量也越多,表明伐桩能够自我稀疏,存在“萌代主”现象.在黄土高原地区,辽东栎伐桩萌芽潜力很大,应合理利用;间伐时伐桩高度应尽量低于20cm;伐桩直径在30～50cm更有利于其无性更新;间伐后伐桩应及时除蘖定株,促进萌生枝生长;间伐强度30.0％(强度间伐)更有利于辽东栎伐桩萌生枝的生长.     

Relationship between stable carbon isotope discrimination and water use efficiency under regulated deficit irrigation of pear-jujube tree

To investigate the relationship between stable carbon isotope discrimination (Δ) of different organs and water use efficiency (WUE) under different water deficit levels, severe, moderate and low water deficit levels were treated at bud burst to leafing, flowering to fruit set, fruit growth and fruit maturation stages of field grown pear-jujube tree, and leaf stable carbon isotope discrimination (Δ_L) at different growth stages and fruit stable carbon isotope discrimination (Δ_F) at fruit maturation stage were measured. The results indicated that water deficit had significant effect on Δ_L at different growth stages and Δ_F at fruit maturation stage. As compared with full irrigation, the average Δ_L at different growth stages and Δ_F at fruit maturation stage were decreased by 1.23% and 2.67% for different water deficit levels, respectively. Δ_L and Δ_F among different water deficit treatments had significant difference at the same growth stage (P < 0.05). Under different water deficit conditions, significant relationships between the Δ_L and WUE_i (photosynthesis rate/transpiration rate, P_n/T_r), WUE_n (photosynthesis rate/stomatal conductance of CO_2, P_n/g_s), WUE_y (yield/crop water consumption, Y/ET_c) and yield, or between the Δ_F and WUE_y and yield were found, respectively. There were significantly negative correlations of Δ_L with WUE_i, WUE_n, WUE_y and yield (P < 0.01) at the fruit maturation stage, or Δ_L with WUE_i and WUE_n (P < 0.01) over whole growth stage, respectively. Δ_F was negatively correlated with WUE_y, WUE_n and yield (P < 0.05), but positively correlated with ET_c (P < 0.01) over the whole growth stage. Thus Δ_L or Δ_F can compare WUE_n and WUE_y, so the stable carbon isotope discrimination method can be applied to evaluate the water use efficiency of pear-jujube tree under the regulated deficit irrigation.     

数字乡村发展的实践与探索——基于北京的调研

研究旨在通过分析北京市数字乡村建设实践,发现存在问题,找出推进路径,为数字乡村发展提供可借鉴经验.利用文献分析、典型案例和专家咨询法,探讨数字乡村发展理论与实践.数字化是现代农业4.0阶段的关键特征,数字经济是农业农村高质量发展的新引擎、新动能.北京是数字乡村发展的先行者,为北京乡村振兴和农业现代化建设打下了坚实基础....     

食品保藏技术课程的教学改革初探

从食品保藏技术课程教学内容的调整、教学方法的选择和实验(实践)教学的改进等方面,针对传统教学模式的缺点和不足,提出了一些思考与看法,旨在为高等农业院校建立科学的教学模式提供一些参考,使学生能更好地掌握该课程。     

SR100垂直摇臂式喷头摇臂有限元分析

针对垂直摇臂式喷头导流器几何结构及运动特性,研究了摇臂在运动过程中的受力及约束情况,并结合动力学理论计算公式,计算和分析了喷头在运动过程中载荷大小和约束情况.采用ANSYS Workbench软件,建立摇臂三维模型,对摇臂进行有限元模态分析、静力学分析及疲劳可靠性分析.通过有限元模态分析,得出各阶模态下摇臂的固有频率和振型,该摇臂的各阶振型主要表现为弯曲、扭曲及局部变形;当摇臂的工作频率小于41.785 Hz时,其振动以一阶振型为主.通过静力分析得出摇臂的应力分布情况,发现在摇臂安装导流器处存在较大的应力分布;通过对摇臂的疲劳分析,发现摇臂各部位安全系数差别很大,其最小值位于摇臂前端,为1.229 1,长期工作此处易发生裂纹或断裂.为提高摇臂的工作稳定性与可靠性,可以通过加强薄弱部位设计,或改进局部结构、提高材料的力学性能来实现.     

冬小麦产量与底墒关系分析

该在分析大量试验资料后指出：在黄土高原南部地区，冬小麦产量与播前底墒相关性强，其相关系数在不同施肥水平下，分别为：中肥处理０．６７７；高肥处理０．７４２；单施氮处理高达０．９６０。因而得出不同施肥处理下的底墒与冬小麦产量关系图，可用于冬小麦产量趋势预测及建立数学模型参考。     

石门国家森林公园风景资源质量等级评定

文章是在对广东省石门国家森林公园风景资源进行全面系统普查的基础上,依据《中国森林公园风景资源质量等级评定》(GB/T 18005-1999)标准,从风景资源质量评价、区域生态状况评价和旅游开发利用条件评价等三个方面对石门国家森林公园进行等级评定,结果表明石门国家森林公园风景质量达到一级,风景资源的资源价值和旅游价值高,评定结果可为公园的保护性开发提供科学依据和理论指导。