MB22木聚糖酶发酵条件的研究

通过因素轮换试验和正交试验,对MB22菌产木聚糖酶的培养基配方和发酵条件进行了研究。结果显示最佳培养基组成是:以玉米芯粉和麸皮为碳源,麸皮120g/l,玉米芯280g/l,(NH4)2SO44g/l,CaCO31.5g/l,Tween80,2.0g/l,MgSO4·7H2O1.5g/l;最佳发酵条件为:起始pH5.0,摇床培养温度30℃,转速160r/min,振荡培养84h。在最佳发酵条件下,MB22菌的最高产酶活力可达898.23U/ml。虽然与一些高产菌株相比该菌株的产酶能力还有待于进一步提高,但该试验结果为进一步进行微生物生产木聚糖酶的研究提供了理论依据。     

奶牛"两病"的监测与思考

奶牛“两病”是指奶牛的结核病和布鲁氏菌病，是世界动物卫生组织(OIE)规定强制报告的两种疫病，二病都属人畜共患病。人的牛型结核感染主要是通过饮用生奶引起，尤其是儿童常因喝生牛奶被感染而患肠结核杆菌病；饮用生奶或制品也易感染布鲁氏菌病。奶牛“两病”严重威胁着人民的身体健     

七种与小麦近缘的野生植物对禾谷缢管蚜抗性的生化机制

研究了7种与小麦近缘的多年生野生植物长穗偃麦草Elytrigia elongata、费尔干偃麦草E. ferganensis、垂穗披碱草Elymus nutans、华山新麦草Psathyrostachys huanshanica、竖立鹅观草Roegneria japonensis、鹅观草R. kamoji和R. tsukushiensis对禾谷缢管蚜Rhopalosiphum padi抗性的生化机制.禾谷缢管蚜内禀自然增长率(rm)与叶片游离脯氨酸和蛋氨酸含量呈极显著和显著正相关,其逐步回归方程为:rm=-0.0198 0.1930X脯氨酸** 0.3350X蛋氨酸* ;总酚含量与禾谷缢管蚜内禀自然增长率无显著相关关系;丁布含量与内禀自然增长率呈极显著的负相关(r=-0.941**,p<0.01).低含量的游离脯氨酸﹑蛋氨酸及高含量的丁布是小麦近缘多年生野生物种抗蚜的重要生化因子.     

早熟桃新品种‘春瑞’

 ‘春瑞’桃系从国外引进品种中选出的优良品种，果实近圆球形，平均单果质量130．5 g，果面全红，果肉白色，含可溶性固形物10．9％ ，果实生育期65 d，早果，丰产。     

早熟油桃‘玫瑰红’选育

玫瑰红油桃是1992年以京玉为母本,五月火为父本进行杂交培育的早熟油桃品种。该品种在郑州6月下旬成熟,果实发育期85d左右;果面75%～100%着玫瑰红色,不裂果;平均单果重150g,最大250g;果肉乳白色,肉质硬溶,果实风味甜,可溶性固形物11%,半离核;需冷量700h,早期丰产性强,极丰产。     

Expression of cyclooxygenase-2 in IL-1β-stimulated mesangial cells is medicated by NF-κB/IκB signal pathway

AIM: To investigate the role of NF-κB/IκB signal pathway in the regulation of cyclooxygenase-2 (COX-2) expression in human mesangial cells (HMC). METHODS: The PGE₂ concentration in supernatants of HMC was measured by radioimmunoassay. COX-2 mRNA and protein expression were determined by RT-PCR and Western blot. Electrophoretic mobility shift assay (EMSA) and Western blot were used to detect the activity of NF-κB and degradation of IκB. RESULTS: IL-1β significantly upregulated COX-2 expression and PGE₂ production in HMC. Significant up-regulation of NF-κB activation, nuclear translocation of p65 subunit, and degradation of IκB α and IκB β were observed in IL-1β-induced HMC. CONCLUSION: Expression of COX-2 in IL-1β-induced HMC is mediated by NF-κB/IκB signal pathway.     

20世纪80年代以来世界除草剂新品种开发进展及特点(一)

综述了近20年来世界除草剂新品种的开发进展，并介绍了除草剂工业发展的一些新特点。     

Effects of component of some Chinese herbs on proliferation of human umbilical vein endothelial cells in vitro

AIM: To observe the effects of some component of Chinese herbs for external use on proliferation of human umbilical vein endothelial cells (HUVEC) and investigate the mechanism of promoting tissue repair. METHODS: The method of MTT was used to examine the effects of Rg1, Rh1, perlolyrine, cinnamyl aldehyde, muscone, astragaluspolysaccharin (APS), velver antler polypeptide (VAP) and soluble extract of boswellia carterii birdw (BCB) on proliferation of HUVEC. RESULTS: APS did not promote proliferation of HUVEC at 9.75 mg/L-2.5 g/L; Rh1 promoted proliferation of HUVEC at 1.94 mg/L-0.5 g/L (P<0.05 or P＜0.01), and Rg1 inhibited proliferation of HUVEC at 31 mg/L (P<0.05); VAP promoted proliferation of HUVEC at 1 mg/L-0.5 g/L with optimal dose of 10 mg/L (P<0.01), Cinnamyl aldehyde promoted proliferation of HUVEC at 2 g/L(P＜0. 05); Muscone and soluble extract of BCB inhibited proliferation of HUVEC at 1 g/L, 0.5-2.5 kg/L(P＜0. 01), respectively; Perlolyrine inhibited proliferation of HUVEC at 0.125 g/L-0.5 g/L(P＜0. 01). CONCLUSION: The external herbs for supplementing Qi and warming Yang can promote HUVEC proliferation and improve angiogenesis during tissue repair. The external herbs for promoting blood circulation and accelerating capillary movement may have influence upon other stages of tissue repair.     

蛋鸡血浆VLDL浓度二世代双向选择对体脂沉积的影响

以雅发褐壳蛋鸡为素材,在零世代和一世代双向选择的基础上,应用血浆极低密度脂蛋白(VLDL)浓度对体腹脂性状继续进行双向选择,比较了高低脂系间体组成的差异,并研究了中后期血浆VLDL浓度与上述性状的相关程度,进一步探讨了血浆VLDL浓度的选择效应。结果表明:二世代母鸡中后期高脂系体重较高(P<0.05),血浆VLDL浓度与体重呈极显著的表型相关(P<0.01)和中等程度的正遗传相关。62周龄高脂系的腹脂率显著高于低脂系(P<0.05),说明运用血浆VLDL浓度经过二个世代的双向选择,使产蛋鸡中后期体重降低,且对腹脂沉积产生了一定的影响。     

Preliminary results of an anticircumsporozoite DNA vaccine trial for protection against avian malaria in captive African black-footed penguins (Spheniscus demersus).

Captive juvenile African black-footed penguins (Spheniscus demersus) housed in an outdoor enclosure at the Baltimore Zoo have an average 50% mortality from avian malarial (Plasmodium sp.) infection each year without intense monitoring for disease and chemotherapeutic intervention. During the 1996 malaria transmission season, the safety and efficacy of an anti-circumsporozoite (CSP) DNA vaccine encoding the Plasmodium gallinaceum CSP protein against P. relictum were studied. The goal was to reduce clinical disease and death without initiating sterile immunity after release into an area with stable, endemic avian malaria. The birds were monitored for adverse clinical signs associated with vaccination, the stimulation of an anti-CSP antibody response, and protection afforded by the vaccine. The presence of P. relictum in trapped culicine mosquitoes within the penguin enclosure was monitored to assess parasite pressure. Among the vaccinated penguins, the parasitemia rate dropped from approximately 50% to approximately 17% despite intense parasite pressure, as determined by mosquito infection rate. During the year of the vaccine trial, no mortalities due to malaria occurred and no undesirable vaccination side effects occurred. This is the first trial of an antimalarial vaccine in a captive penguin colony.