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111.
Robert A. Spotts Kelly M. Wallis Maryna Serdani Daniel T. O’Gorman Peter L. Sholberg 《Postharvest Biology and Technology》2009,51(2):227-231
The objectives of this study were to develop primers and a real time PCR protocol for the postharvest biocontrol yeast Cystofilobasidium infirmominiatum (Cim). The application of this technology was developed to quantify Cim on the surfaces of apple, two pear cultivars, and sweet cherry fruit treated over a range of concentrations. Statistically significant relationships were observed between Cim DNA on fruit surfaces, expressed as μg/m2, and CFU/L of dip suspensions for apple, pear, and sweet cherry. In addition, the relationship for each fruit was significantly different from the other three fruits. Threshold values of concentrations of Cim DNA on the fruit surface were calculated based on regression equations and a dose of 2.0 × 1011 CFU/L of dip suspension, the dose for optimum decay control, and were 4.8, 7.0, 16.5, and 25.2 μg/m2 for Bosc pear, Lapins sweet cherry, d’Anjou pear, and Golden Delicious apple, respectively. Monitoring Cim DNA concentration on fruit surfaces will assure that Cim is being properly applied to fruit and that a sufficient number of cells are present for optimum decay control. 相似文献
112.
Multi-attribute evaluation of landscape-level fuel management to reduce wildfire risk 总被引:1,自引:0,他引:1
Dan W. Ohlson Trent M. Berry Robert W. Gray Bruce A. Blackwell Brad C. Hawkes 《Forest Policy and Economics》2006,8(8):824-837
This paper provides an example of the practical application of multi-attribute trade-off analysis (MATA) to wildfire management. The MATA approach supports more informed decision-making because it exposes important trade-offs among competing management objectives (requiring value-based choices), helps guide and structure necessary technical judgements, explicitly represents uncertainty (i.e., not just expected outcomes but risk profiles around outcomes) and addresses temporal trade-offs. MATA promotes critical thinking about what analysis is required for decision-making. A MATA approach can be applied for all types of forest and fire management decisions. In this paper, we provide a sample application of MATA to an evaluation of landscape-level fuel treatments for managing wildfire risk. The study area is located in southeastern British Columbia, Canada where historical fire suppression policies and expanding development in wildland urban interface areas have resulted in an increase in both the probability and the consequences of stand replacement fires. We specify management objectives and develop measurable attributes for fire management costs, timber supply, property damage, landscape-level biodiversity, local air quality and climate change. We then simulate the effects on these attributes of four alternative fuel management strategies that include combinations of mechanical treatments and prescribed burning over a 100-year period. The evaluation illustrates the key features of MATA while highlighting the benefits and challenges of implementing the approach. 相似文献
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114.
Luke Randall Katharina Heinrich Robert Horton Lucy Brunton Matthew Sharman Victoria Bailey-Horne Meenaxi Sharma Ian McLaren Nick Coldham Chris Teale Jeff Jones 《Research in veterinary science》2014
Waste milk samples from 103 farms in England and Wales were examined for the presence of β-lactam antibiotics and ESBL-producing Enterobacteriaceae. Approximately 10 months after the initial sampling, further waste milk, environmental and faecal samples from farms shown to be positive for CTX-M Escherichia coli were investigated further. Isolates with an ESBL phenotype were tested by PCR for the presence of blaCTX-M, blaOXA, blaSHV and blaTEM genes. Isolates positive for blaCTX-M were sequenced to determine CTX-M type. Representative isolates were further examined by PFGE, plasmid replicon typing and serotyping. Of particular interest, 21.4% of waste milk samples contained residues of the cephalosporin cefquinome, which was significantly associated with CTX-M bacteria. Such bacteria occurred in 5.8% of the waste milk samples (including 3.9% CTX-M E. coli). CTX-M types identified were 1, 14, 14b and 15, but none of the E. coli were serotype O25, the serotype of the human pandemic strain. 相似文献
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J. H. Heckman H. G. Zuckerman C. H. Metzger W. B. Ward John S. Gardner Frank W. Hussey Ralph W. Donaldson A. G. Tolaas K. H. Fernow E. V. Hardenburg W. G. Been Robert Schmidt E. M. Gillig E. B. Tussing K. W. Lauer K. H. Klages Brooks D. Drain H. L. Bailey J. G. Milward 《American Journal of Potato Research》1935,12(7):196-204
118.
J. H. Heckman H. G. Zuckerman C. H. Metzger B. A. Brown E. R. Bennett W. B. Ward C. L. Fitch O. H. Elmer Jhon S. Gardner Julan C. Miller Frank W. Hussey E. L. Newdick Ralph W. Donaldson Lionel Harris H. O. Werner Wm. H. Martin E. V. Hardenburg T. E. Odland Robert Schmidt Geo E. Prince Brooks D. Drain Harold L. Bailey G. S. Ralston Chase D. Gaines J. G. Milward John Tucker 《American Journal of Potato Research》1935,12(6):158-170
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120.
John T. Hathcock DVM MS D. G. Pugh DVM MS Robert E. Cartee DVM MS Linda Hammond DVM 《Veterinary radiology & ultrasound》1995,36(4):290-296
Computed tomography was performed on the head of 6 normal adult llamas. The animals were under general anesthesia and positioned in dorsal recumbency on the scanning table. The area scanned was from the external occipital protuberance to the rostral portion of the nasal passage, and the images are presented in both a bone window and a soft tissue window to allow evaluation and identification of the anatomy of the head. Computed tomography of the llama head can be accomplished by most computed tomography scanners utilizing a technique similar to that used in small animals with minor modification of the scanning table. 相似文献