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101.
利用PCR特异性引物检测了一株对相似穿孔线虫具有高拮抗活性的Burkholderia cepacia菌株HD-86的毒性,结果未检测到该菌株中与人体致病相关的洋葱伯克霍尔德氏菌致病因子(BCESM)毒力基因,从而验证了其对人类和环境的安全性.菌株HD-86发酵上清液对相似穿孔线虫拮抗活性稳定性的研究结果表明,经80℃高温处理60 min后其拮抗活性仍然极高,在pH为8~10的范围内随着碱性增强其拮抗性效果越好,紫外线处理对其拮抗活性没有影响. 相似文献
102.
Methyl donors dietary supplementation to gestating sows diet improves the growth rate of offspring and is associating with changes in expression and DNA methylation of insulin‐like growth factor‐1 gene 下载免费PDF全文
Yang Zhao Yuedong Xuan Daolin Mou Hong Liu Pan Zhou Zhengfeng Fang Lianqiang Che Shengyu Xu Bin Feng Jian Li Xuemei Jiang Yan Lin De Wu 《Journal of animal physiology and animal nutrition》2018,102(5):1340-1350
The study aimed to investigate the effects of maternal dietary methyl donors on the performance of sows and their offspring, and the associated hepatic insulin‐like growth factor‐1 (IGF‐1) expression of the offspring. A total of 24 multiparous sows were randomly fed the control (CON) or the CON diet supplemented with methyl donors (MD) at 3 g/kg betaine, 15 mg/kg folic acid, 400 mg/kg choline and 150 μg/kg VB12, from mating until delivery. After farrowing, sows were fed a common lactation diet through a 28‐days lactation period and six litters per treatment were selected to be fed until at approximately 110 kg BW. Maternal MD supplementation resulted in greater birthweight (p < 0.05) and increased the piglet weights (p < 0.01) and litter weights (p < 0.05) at the age of day 28, compared with that in CON group. The offspring pigs in the MD group had greater ADG (p < 0.05) and tended to lower F:G ratio (p = 0.07) compared with that of CON group from day 28 to 180 of age. The offspring pigs from MD group had greater serum IGF‐1 concentrations and expressions of hepatic IGF‐1 gene and muscular IGF‐1 receptor (IGF‐1r) protein at birth (p < 0.05), and greater hepatic IGF‐1 protein (p = 0.03) and muscular IGF‐1r gene expressions (p < 0.05) at slaughter, than that from the CON group. Moreover, the methylation at the promoter of IGF‐1 gene in the liver of newborn piglets and finishing pigs was greater in the MD group than that of the CON group (p < 0.05). In conclusion, maternal MD supplementation throughout gestation could enhance the birthweight and postnatal growth rate of offspring, associated with an increased expression of the IGF‐1 gene and IGF‐1r, as well as the altered DNA methylation of IGF‐1 gene promotor. 相似文献
103.
104.
为了提高养鳖经济效益,除了要抓好生产技术和销售外,如何缩短养殖周期,提高现有设备的利用率,已成为提高养鳖效益的重要途径。现把通过多年实践的工厂化两年养三茬成鳖新技术介绍如下,供有条件的地方参考应用。一、两年养三连成鳖的基本条件1.温室要求保温好,有供热供水条件(水质要附合养殖标准)。2.鳖池不宜太大,一般在25平方米,池深50厘米,水深35厘米,他中设饲料台(饲料台可用优质水泥瓦,不能用石棉瓦向采用无涉或沙网结合养殖,可在池中挂几趟无结网片作隐蔽物。3往排水方便C二、两年养三茬成鳖的工艺流程所谓两年养王茬… 相似文献
105.
106.
不同生长模型估计籽鹅早期体重发育规律及遗传参数 总被引:8,自引:0,他引:8
运用Logistic,Gompertz,VonBertalanfy和Cubic4种非线性生长模型对籽鹅1~1周龄的平均体重进行拟合,并分析生长发育规律及遗传参数。结果表明:虽然4个模型都能描述籽鹅的生长过程,拟合度均超过额0.99;但Logistic模型更符合实际饲养情况,能更好地预测体重,为最佳模型,其相应的体重极限参数A为2267.63g,调节参数B为16.27,瞬时相对生长率C为0.47,生长的拐点时间为6.48周龄,拐点体重为1136.81g。 相似文献
107.
Ferreira SR Araújo JV Braga FR Araujo JM Carvalho RO Silva AR Frassy LN Freitas LG 《Tropical animal health and production》2011,43(3):639-642
The ovicidal effect of the nematophagous fungus Pochonia chlamydosporia on eggs of Ascaris suum was tested under laboratory conditions. A. suum eggs were plated on 2% water–agar with seven fungal isolates (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4)
and control without fungus. After 5, 7, 10, 14, 15 and 21 days of incubation, approximately 100 eggs were removed from the
plates and classified according to the following parameters: type 1, biochemical and physiological effect without morphological
damage to the eggshell, type 2, lytic effect with morphological alteration of the eggshell and embryo and type 3, lytic effect
with morphological alteration of eggshell and embryo showing hyphal penetration and internal egg colonization. The isolates
effectively destroyed A. suum eggs and all types of effects were observed during the experiment. There was no variation in ovicidal capacity (type 3 effect)
among the isolates (p > 0.05) throughout the experiment. After 21 days, isolate 5 showed the highest percentages of type 3 effect (58.33%). The
results indicated that P. chlamydosporia (Isol. 5, Isol. 31, Isol. 1, VC1, Isol. 12, Isol. 22 and VC4) can destroy A. suum eggs and is, therefore, a potential biological control agent of nematodes. 相似文献
108.
Jemberu Dessie Solomon Melaku Firew Tegegne Kurt J. Peters 《Tropical animal health and production》2010,42(5):841-848
The experiment consisting of 7 days of digestibility and 90 days of feeding trial was conducted at Wogda (Ethiopia) to determine
the effect of supplementation of graded levels of concentrate mix (CM) on feed intake, digestibility, and body weight (BW)
change in hay-based feeding of Simada sheep. Twenty-yearling Simada sheep with a mean initial BW of 17.9 ± 0.81 kg (mean ±
SD) were used in randomized complete block design arranged into five blocks of four animals. The four dietary treatments that
consisted of hay alone (T1), hay +150 g dry matter (DM; T2, low), hay +250 g DM (T3, medium), and hay +350 g DM (T4, high)
CM were randomly assigned to each sheep within a block. The CM consisted of wheat bran (WB), noug seed (Guizotia abyssinica) meal and safflower (Carthamus tinctorius) seed meal at the ratio of (2:1:1), respectively. Supplementation with T2 and T3 increased (P < 0.001) total DM and organic matter intake than the control treatment. Overall, supplementation improved (P < 0.001) crude protein intake, digestibility, feed conversion efficiency, BW gain, and profitability compared to the control,
whereas sheep on the high than the low and medium level of supplementation performed better in these parameters among the
supplemented treatments. From the results of this study, T4 is recommended as the best level of supplementation since it resulted
in better nutrient utilization, animal performance, and profitability. 相似文献
109.
马铃薯立枯丝核菌生物学特性研究 总被引:3,自引:0,他引:3
采用生长速率法研究了黑龙江省马铃薯立枯丝核菌(Rhizoctonia solani Kühn)菌丝生长的条件。结果表明病菌菌丝在10~35℃均能生长,适宜温度是25~30℃,30℃菌丝生长最快。在pH为3~12的范围内病菌菌丝均能生长,以pH 5~7菌丝生长速度较快。黑暗对菌丝生长有促进作用,光照可促进菌核的形成。不同碳源、氮源对立枯丝核菌菌丝生长的影响均较明显,可溶性淀粉和酵母粉对菌丝生长有显著的促进作用。立枯丝核菌菌丝体及菌核的致死温度分别为49℃和51℃。明确了黑龙江省马铃薯立枯丝核菌生长的环境条件,可为病害防治提供理论依据。 相似文献
110.