Liquid Storage of Goat Semen in Chemically Defined Extenders

The suitability of certain commercial and self‐made chemically defined extenders for liquid storage of goat semen was tested and the effects of storage temperatures, dilution rates and sperm washing and pH of extenders on the goat sperm during liquid storage were observed. Semen was collected from nine goat bucks of the Lubei White and Boer breeds using an artificial vagina. Each ejaculate after initial evaluation was diluted with a specific extender, cooled and stored at a desired temperature. Stored semen was evaluated for sperm motility and other parameters every 24 or 48 h of storage. The ranking order of the existing milk‐ and yolk‐free extenders in sustaining goat sperm motility was Androhep > Zorlesco > Beltsville thawing solution > the Tris–glucose medium. The new extender (mZA) which was formulated based on Zorlesco and Androhep was more suitable for goat sperm than Androhep. The mZAP extender with Bovine Serum Albumin (BSA) replaced with polyvinyl alcohol (PVA) worked as efficiently as the mZA in maintaining sperm motility, membrane integrity, acrosome intactness and capacitation status. Goat sperm motility was best maintained at 5°C during liquid preservation, but decreased significantly as the temperature increased. When semen was sixfold diluted, sperm motility was maintained longer (p < 0.05) after centrifugation, but sperm motility did not differ between the centrifuged and non‐centrifuged groups when semen was 11‐fold diluted. When the extender pH was adjusted from 6.6 to 6.04, the efficiency increased significantly in both Androhep and mZAP. A forward sperm motility of 34% was maintained for 9 days when buck semen was 11‐fold diluted and stored at 5°C in mZAP, with pH adjusted to 6.04. It is concluded that for liquid storage of buck semen, the mZA extender was more suitable than other extenders; BSA can be replaced with PVA in mZA; centrifugation to remove seminal plasma can be omitted by adequate dilution; and the storage temperature and pH of extenders affected sperm motility significantly.     

广西狂犬病野毒株L基因3'端的多态性分析

为了解狂犬病病毒(RV)的变异情况,本研究对广西不同地区的22个RV分离株的L基因3'端片段进行克隆和测序,与国内外发表的RV街毒、固定毒株及类RV株相应部分的多态性进行了比较分析.结果表明.所测定的22个广西RV野毒分离株属于基因I型,可分为3个群即I群、Ⅱ群和Ⅲ群.其中13株属于I群,其核苷酸同源性为96.9%～100.0%,氨基酸同源性为98.2%～100.0%;8株属于Ⅱ群,株核苷酸同源性为96.5%～99.7%.氨基酸同源性为97.8%～100.0%.仅1株属于Ⅲ群.22个RV分离株与RV固定毒株核苷酸和氨基酸的同源性分别为79.5%～86.9%和85.8%～96.5%,与类RV核苷酸和氨基酸的同源性分别为65.7%～70.3%和70.4%～76.5%.     

法庭回答模糊限制语的探析及对我国法学教育的思考

模糊限制语被广泛应用于法庭话语中。文中采用Prince对模糊限制语的分类方法,对法庭回答中的模糊限制语及其会话含意进行分析,旨在说明模糊限制语在法庭回答中发挥着重要的作用,能够使回答具有丰富的会话含意并进而影响法庭审判结果。因而应引起司法人员的关注,在庭审中予以恰当处理。最后对我国法学教育进行了一些思考。     

犊牦牛大脑的形态学观察

通过对3月龄牦牛大脑进行形态学观察,为进一步探讨牦牛神经系统对高寒低氧环境适应性方面的研究提供形态学资料.运用大体解剖学方法对4头3月龄牦牛的大脑进行解剖、观察、描述、绘图.结果表明,犊牦牛大脑表面平坦,沟回分界不明显:大脑前半部较平直,但在大脑背外侧中部,突然向两侧膨大加宽;大脑外侧窝为一个短小的沟,有的较深而有的较浅.视交叉明显呈钝角.由此说明,3月龄牦牛大脑的形态与成年牦牛的大脑相比有明显区别.     

Stripe rust resistance in <Emphasis Type="Italic">Aegilops tauschii</Emphasis> and its genetic analysis

Aegilops tauschii Coss., the D-genome progenitor of common wheat (Triticum aestivum L.) includes two subspecies, tauschii and strangulata (Eig) Tzvel. Subspecies tauschii has a wide geographic distribution spreading westwards to Turkey and eastwards to Afghanistan and China, while ssp. strangulata has a narrower distribution occurring only in two disjoined regions, southeastern Caspian Iran and Transcaucasia. A collection of 56 Ae. tauschii accessions was screened at adult stage against a mixture of pathotypes of stripe rust prevalent in the current wheat production in China. The results for three crop seasons indicated that among the 38 ssp. tauschii accessions, 37 were susceptible and only one was resistant, while all the 18 ssp. strangulata accessions were resistant. These results indicated that stripe rust resistance was related to taxonomic origin. Further genetic analysis revealed the resistance of stripe rust in ssp. strangulata accession AS2388 was conferred by a single dominant gene.     

南阳牛Gli3基因多态性及其与生长性状的相关性

Gli3基因是Gli-Kruppel家族中的一员,其参与的Sonic hedgehog信号通路在动物生长发育,特别是早期生长发育过程中有重要作用.利用PCR-SSCP和DNA测序技术研究了187头纯种南阳牛Gli3基因编码区4个位点(E4、E5、E6和E7)的多态性,发现E4和E5位点存在SSCP多态.对不同SSCP带型的对应片段进行了测序分析,共发现3个新的SNP多态位点(T123600C、T123696C和A128688G),其中第123600和第123696两个位点处于连锁状态.E5位点处于Hardy-Weinberg平衡状态,E4位点处于非平衡状态.不同基因型与生长发育性状的相关性分析显示,E4位点上,CC基因型个体的六月龄体重、胸围、坐骨端宽、日增重指标显著大于TT基因型个体(P<0.05),该位点有可能作为南阳牛早期生长性状标记辅助选择的标记之一.     

深海鱼油调和油煎炸食物时脂肪酸和相关理化参数的动态变化

研究含不同剂量深海鱼油调和油(fish oil supplemented blend oil,FO)煎炸食物时其脂肪酸及相关参数的动态变化。分别用2L大豆调和油(soybean blend oil,SO)与1.5%、3.0%、6.0%和12.0%FO在油温180℃条件下煎炸新鲜薯条,每次煎炸120g,煎炸时间为3min,每2次煎炸间隔5min,每日煎炸结束后保温1.5h;每日重复10次,共煎炸6d;用气相色谱法测定样品油的脂肪酸组分,用《食用植物油卫生标准》中的分析方法测定理化参数。结果表明:煎炸过程中生成的主要反式脂肪酸(trans fatty acids,TFAs)为t18:1、t18:2n-6、t18:3n-6以及t18:3n-3;随煎炸时间的延长,其产生的t18:1与t18:2n-6的组分呈上升趋势,且显著高于SO(P<0.05);t18:3n-6与t18:3n-3组分在3.0%及6.0%FO中随煎炸时间的延长呈先升后降的趋势,而在SO、1.5%和12.0%FO中均呈下降趋势;在不同剂量的FO中20:5n-3与22:6n-3组分随煎炸时间的延长而降低;SO的酸价、茴香胺值以及聚合物等均显著低于FO(P<0.01),但FO的过氧化值显著低于SO(P<0.01)。研究结果表明FO与SO可安全用于烹饪,但均不适宜长时间反复煎炸。     

冻融循环条件下SBS改性沥青混合料的水稳定性研究

在我国北方寒冷地区,冬、春季更替时沥青路面材料长时间处于含水、低温冰冻状态及温度交替变化过程中,因水分和温度的共同作用引起的冻融循环而导致路面开裂现象较为普遍.本文对SBS改性沥青混合料试件进行多次冻融循环后进行劈裂试验,利用空隙率和冻融劈裂抗拉强度随冻融循环次数增加的变化规律来评价SBS沥青混合料的水稳定性.研究结果表明:SBS改性沥青混合料的强度及水稳定性受多次反复冻融影响衰减显著.     

云南省不同烟区K326烟叶的常规化学成分比较(英文)

[目的]比较云南不同烟区K326B2F烟叶常规化学成分的地区差异性。[方法]常规化学成分采用连续流动分析仪检测,并利用SPSS17.0统计软件包进行数据的统计分析和方差分析。[结果]不同地区K326的各项常规化学成分指标大多存在显著差异,其中总糖、还原糖和烟碱差异达到了极显著水平;钾和总氮差异达到了显著水平,氯则无显著差异。玉溪地区的K326B2F烟叶大部分常规化学成分与曲靖、大理和楚雄间均存在显著差异。滇东、滇中和滇西3个烟区的总糖、还原糖和烟碱含量的差异明显;而同属滇西的楚雄和大理间常规化学成分含量差异并不明显,这说明云南省不同大区域间生态环境的差异对烟叶的影响较大。[结论]揭示了云南省K326初烤烟叶常规化学成分特性的区域特征,为卷烟配方和特色优质烟叶的生产提供了参考依据。     

吉林省乾安县学校防雷现状分析与防御对策

从乾安县内学校的防雷设备出发,进行了详尽的现状分析,近而从装置防雷设备,机房建设以及运动场的防雷装置等方面,提出了相应的防御对策.