全文获取类型
收费全文 | 16439篇 |
免费 | 1篇 |
专业分类
林业 | 3640篇 |
农学 | 1299篇 |
基础科学 | 137篇 |
2739篇 | |
综合类 | 706篇 |
农作物 | 2095篇 |
水产渔业 | 1778篇 |
畜牧兽医 | 1071篇 |
园艺 | 1110篇 |
植物保护 | 1865篇 |
出版年
2021年 | 2篇 |
2020年 | 5篇 |
2019年 | 2篇 |
2018年 | 2745篇 |
2017年 | 2706篇 |
2016年 | 1178篇 |
2015年 | 64篇 |
2014年 | 17篇 |
2013年 | 10篇 |
2012年 | 793篇 |
2011年 | 2122篇 |
2010年 | 2104篇 |
2009年 | 1253篇 |
2008年 | 1313篇 |
2007年 | 1574篇 |
2006年 | 32篇 |
2005年 | 97篇 |
2004年 | 101篇 |
2003年 | 152篇 |
2002年 | 60篇 |
2001年 | 6篇 |
2000年 | 41篇 |
1999年 | 1篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1993年 | 13篇 |
1992年 | 7篇 |
1990年 | 1篇 |
1989年 | 5篇 |
1988年 | 13篇 |
1987年 | 1篇 |
1977年 | 4篇 |
1972年 | 1篇 |
1969年 | 1篇 |
1968年 | 4篇 |
1967年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
131.
Lounis Mohamed Zhao Ge Li Yuehua Gao Yubin Kaidi Rachid Oumouna Mustapha Wang Junwei Oumouna Karine 《Tropical animal health and production》2018,50(3):547-553
Avian pathogenic E. coli (APEC) is the etiologic agent of avian colibacillosis, the most common disease responsible for chicken morbidity in the world. Although multiple virulence-associated factors were identified, their prevalence in Algeria is still poorly known. In the present research, 92 avian pathogenic E. coli (APEC) isolates were recovered from broilers with clinical signs and lesions of colibacillosis. In addition, 32 E. coli isolates collected from feces of healthy birds (AFEC) were included for comparison. All isolates were investigated by PCR for the presence of a total of 11 virulence-associated genes described for avian pathogenic (iroN, ompT, hlyF, iss, iutA, and fimC) and diarrheagenic E. coli (eae, stx, elt/est, ipaH, and aggR). The sensitivity of 39 APEC isolates to 16 antibiotics was also determined using antimicrobial pretreated microplates. Here, we report that 98% of the examined isolates host at least one of the tested virulence factors. The most prevalent genes in APEC were iutA (90.6%), ompT (86.9%), and iss (85.8%); whereas, iutA (78.1%), fimC (78.1%), and iroN (68.7%) were the highest prevalent genes in AFEC. Our data showed that none of the AFEC isolates harbor any of the tested diarrheagenic genes. Moreover, only elt/est (5.4%), stx (2.1%), and ipaH (2.1%) genes were carried by APEC isolates. We further established that ceftazodime, ceftiofur, mequindox, amoxicillin/clavulanic acid, and meropenem were the most efficient antibiotics against the analyzed APEC isolates. Overall, our findings provide more insights about APEC and AFEC virulence potential in Algeria which could participate in the fight against colibacillosis. 相似文献
132.
133.
Solvent-extracted soybean meal (SBM) was fermented using baker’s yeast Saccharomyces cerevisae at 30 °C for 5 days. Four isonitrogenous and isocaloric diets containing 75% SBM protein, either fermented or non-fermented (SBM and FSBM), and supplemented or not with organic Se (OS) (SBMOS and FSBMOS), were fed to triplicate groups of juvenile barramundi (Lates calcarifer) (initial weight of 5 g) for 75 days. A fishmeal (FM)-based diet formulated for juvenile barramundi was used as a reference diet. The growth of fish was significantly affected by either the interaction of SBM type or by the OS level. In fish fed diets supplemented with OS (SBMOS and FSBMOS), final weight (FW), specific growth rate (SGR) and weight gain (WG) were higher in fish fed the fermented SBM (FSBMOS) than in those fed the non-fermented SBM (SBMOS). The apparent digestibility coefficient (ADC) of protein was higher in the fish fed the fermented SBM, either supplemented or unsupplemented with OS. However, there were no significant differences in the ADC of dry matter (DM) and lipids among the tested diets and in comparison to the reference diet. The haematocrit and leucocrit of fish fed the FSBMOS diet were lower than those of fish fed the FM diet. Furthermore, glutathione peroxidase (GPx) activity was significantly influenced by OS supplementation in the experimental diets; GPx activity was greater in the fish fed diets supplemented with OS. Creatinine kinase (CK) of all groups of fish was higher than the CK of those fed the reference diet. These results suggest that with a proper nutritional level, OS supplementation may act as an important factor in enzymatic GPx activity and in the haematology and blood biochemistry status of juvenile barramundi fed fermented SBM-based diets, encouraging improvement of the overall growth performance. 相似文献
134.
Carbon nanotube (CNT)/magnetite (Fe3O4) nanocomposites were successfully fabricated by nanocoating. When the CNT/Fe3O4 nanocomposites were added to a carbonyl (CI) based magnetorheological fluid (MRF), the magnetic rheological properties of
the MRF were enhanced. Such enhancement in magnetic rheological properties of the CI-CNT/Fe3O4 MRF is considered to be attributable to both the superparamagnetic property of the magnetic layer covering the CNT surface
and the high length to diameter ratio of the CNT/Fe3O4 nanocomposite. Impact properties for CI-CNT/Fe3O4 MRF treated Kevlar fabrics were also tested, and improved impact properties were observed with external magnetic fields compared
to CI-MRF treated Kevlar fabrics. 相似文献
135.
136.
Effects of silviculture treatments in a hurricane-damaged forest on carbon storage and emissions in central Hokkaido,Japan 总被引:1,自引:0,他引:1
Hurricanes cause abrupt carbon reduction in forests, but silviculture treatment can be an effective means of quickly regenerating and restoring hurricane-damaged sites. This study assessed how silviculture treatments affect carbon balance after hurricane damage in central Hokkaido, Japan. We examined carbon storage in trees and underground vegetation as well as carbon emissions from silviculture operations in 25-year-old stands, where scarification and plantation occurred just after hurricane damage. The amount of carbon stored varied according to silviculture treatment. Among three scarification treatments, a scarified depth of 0 cm (understory vegetation removal) led to the largest amount of carbon stored (64.7 t·ha-1 C). Among four plantation treatments, the largest amount of carbon was stored in a Larix hybrid (L. gmelinii var. japonica × L. kaempferi) plantation (80.3 t·ha-1 C). The plantation of Abies sachalinensis was not successful at accumulating carbon (40.5·ha-1 C). The amount of carbon emitted from silviculture operations was 0.05-0.14 t·ha-1 C, and it marginally affected the net carbon balance of the silviculture project. Results indicate that silviculture treatments should beperformed in an appropriate way to effectively recover the ability of carbon sequestration in hurricane-damaged forests. 相似文献
137.
Previous work in the agri-food tradition has framed food auditing as a novelty characteristic of a shift to neoliberal governance
in agri-food systems and has tackled the analysis of food “quality” in the same light. This article argues that agri-food
scholars’ recent interest in the contested qualities of food needs to be situated alongside a much longer history of contested
cultural attributions of trust in food relations. It builds on an earlier discussion suggesting that, although neoliberalism
has undoubtedly opened up new spaces for audit activity, older political and social dynamics operating around food audits
were established long before the neoliberal historical moment. Breaking new ground (as far as is known) by looking further
back than the early history of the organic social movement, it examines intersections of religious food auditing, migrant
food culture, and commercial dynamics in food systems. Based on secondary sources, two contrasting case studies are presented
to illustrate the flux and complexity for: New World Diaspora migrants to New York City of assuring food was kosher; and more recent Maghrebi migrants to southwest France of assuring food is halal. The article concludes by noting that the neoliberal moment stands not as the unique progenitor of a new style of food authority,
but rather as the latest response to a wider rupture in the historically contingent arbitration of new forms of trust in food. 相似文献
138.
Yong Hoon Lee Seungdon Lee Dong Hee Lee Sang Hye Ji Hyun Young Chang Sunggi Heu Jae Wook Hyun Dong-Soo Ra Eun Woo Park 《European journal of plant pathology / European Foundation for Plant Pathology》2008,120(1):97-102
Soil-borne cereal mosaic virus (SBCMV) causes a severe disease in susceptible cultivars of winter wheat. The virus is vectored by the soil-borne protist
Polymyxa graminis. Experiments were conducted to investigate whether SBCMV RNA2 could persist in seed from SBCMV-infected susceptible cultivars
of winter wheat. Over 7,000 seedlings were generated from seed collected from two cultivars of SBCMV-infected winter wheat.
Seedlings were grown in a glasshouse compartment and batch tested for the presence of SBCMV using real-time RT-PCR. The majority
of batches tested positive for SBCMV, indicating an RNA2 transmission rate of 1.8–9.4% in wheat. The presence of the virus
was confirmed by amplifying and sequencing a larger (400 bp) fragment of viral RNA2 in a sub-set of the seedlings testing
positive by real-time RT-PCR. Root extracts from this sub-set tested negative for P. graminis using real-time PCR. The implications for disease epidemiology of this virus are discussed.
The authors are British Civil Servants and as such their work is subject to British Crown Copyright. This means the exclusive
copyright for the article cannot be transferred. 相似文献
139.
Kapil Malik Deepshikha Birla Honey Yadav Manish Sainger Darshna Chaudhary Pawan K. Jaiwal 《Journal of Crop Science and Biotechnology》2017,20(3):185-192
Various factors affecting in vitro regeneration like different carbon sources, different gelling agents, and growth additives were assessed comprehensively for callus induction and plant regeneration for five Indian wheat cultivars using mature embryos as the explants for the first time. The tissue culture responses of cultivars WH-1105, HD-2967, and PBW-343 have not been reported earlier. Besides, the effect of different concentrations of the cytokinin, zeatin has also been optimized. Using the optimized factors, the efficiency of five different varieties, i.e., HD 2967, C 306, RAJ 3765, WH 1105, and PBW 343 was evaluated for regeneration. Modified MS basal medium containing dicamba reduced precocious germination of the embryo and induced embryogenic callus more efficiently. Removal of embryogenic calli from non-regenerable structures during early callus phase improved plant regeneration. These calli on zeatin (1.0 mgl-1) and dicamba (0.1 mgl-1) containing medium showed the highest regeneration frequency (98%) with a maximum of 8-9 shoots per calli. Maltose had the maximum callusing and regeneration percentage than other carbon sources. Various gelling agents did not have any significant difference on the regeneration. Of all the varieties, C-306 and HD-2967 were found to be more regenerative and can be used in transformation experiments. 相似文献
140.
Mi Jeong Park Jienny Lee Jeong Su Byeon Da-Un Jeong Na-Yeon Gu In-Soo Cho Sang-Ho Cha 《Veterinary research communications》2018,42(3):171-181
Mesenchymal stem cells (MSCs) are useful candidates for tissue engineering and cell therapy fields. We optimize culture conditions of equine adipose tissue-derived MSCs (eAD-MSCs) for treatment of horse fractures. To investigate enhancing properties of three-dimensional (3D) culture system in eAD-MSCs, we performed various sized spheroid formation and determined changes in gene expression levels to obtain different sized spheroid for cell therapy. eAD-MSCs were successfully isolated from horse tailhead. Using hanging drop method, spheroid formation was generated for three days. Quantitative real-time PCR was performed to analyze gene expression. As results, expression levels of pluripotent markers were increased depending on spheroid size and the production of PGE2 was increased in spheroid formation compared to that in monolayer. Ki-67 showed a remarkable increase in the spheroid formed with 2.0?×?105 cells/drop as compared to that in the monolayer. Expression levels of angiogenesis-inducing factors such as VEGF, IL-6, IL-8, and IL-18 were significantly increased in spheroid formation compared to those in the monolayer. Expression levels of bone morphogenesis-inducing factors such as Cox-2 and TGF-β1 were also significantly increased in spheroid formation compared to those in the monolayer. Expression levels of osteocyte-specific markers such as RUNX2, osteocalcin, and differentiation potential were also significantly increased in spheroid formation compared to those in the monolayer. Therefore, spheroid formation of eAD-MSCs through the hanging drop method can increases the expression of angiogenesis-inducing and bone morphogenesis-inducing factors under optimal culture conditions. 相似文献