全文获取类型
收费全文 | 25140篇 |
免费 | 1547篇 |
国内免费 | 2132篇 |
专业分类
林业 | 2022篇 |
农学 | 1691篇 |
基础科学 | 1101篇 |
2451篇 | |
综合类 | 12101篇 |
农作物 | 1698篇 |
水产渔业 | 991篇 |
畜牧兽医 | 3754篇 |
园艺 | 1877篇 |
植物保护 | 1133篇 |
出版年
2024年 | 143篇 |
2023年 | 458篇 |
2022年 | 1107篇 |
2021年 | 1134篇 |
2020年 | 1126篇 |
2019年 | 1073篇 |
2018年 | 744篇 |
2017年 | 1222篇 |
2016年 | 849篇 |
2015年 | 1341篇 |
2014年 | 1350篇 |
2013年 | 1603篇 |
2012年 | 2190篇 |
2011年 | 2130篇 |
2010年 | 1963篇 |
2009年 | 1842篇 |
2008年 | 1751篇 |
2007年 | 1624篇 |
2006年 | 1352篇 |
2005年 | 1089篇 |
2004年 | 654篇 |
2003年 | 370篇 |
2002年 | 443篇 |
2001年 | 380篇 |
2000年 | 364篇 |
1999年 | 162篇 |
1998年 | 54篇 |
1997年 | 51篇 |
1996年 | 36篇 |
1995年 | 39篇 |
1994年 | 21篇 |
1993年 | 33篇 |
1992年 | 30篇 |
1991年 | 13篇 |
1990年 | 18篇 |
1989年 | 12篇 |
1988年 | 11篇 |
1987年 | 9篇 |
1986年 | 2篇 |
1985年 | 1篇 |
1984年 | 1篇 |
1981年 | 5篇 |
1980年 | 1篇 |
1963年 | 1篇 |
1962年 | 4篇 |
1956年 | 9篇 |
1955年 | 4篇 |
排序方式: 共有10000条查询结果,搜索用时 62 毫秒
51.
阐释了园林构成要素中的必然性要素和偶然性要素及其相互关系与运用意义;以昆明世博园中“齐鲁园”为例,辩识了“齐鲁园”中必然性与偶然性园林要素,分析了诸要素在特定园林环境布局中的运用。 相似文献
52.
AIM:To establish cell line FL-POLκ- and to study the role ofPOLκ(polymerase kappa) on genetic stability.METHODS:A mammalian expression vector expressing antisense POLκ gene fragment pMAMneo -amp--POLκ was constructed by cloning the 1 690-1 918 fragment of POLκ gene into the mammalian expression vector pMAMneo-amp- in antisense orientation. FL cells were fransfected with this antisense RNA expressing vector and selected by G418. Based on the shuttle-plasmid pZ189, the mutation assay was made.RESULTS:The spontaneous mutation frequency of supF tRNA gene in the plasmid replicated in the FL- POLκ- was 11.2×10-4, while it was 4.9×10-4 and 3.7×10-4 in the control cells FL and FL-M, respectively.CONCLUSION: POLκ playes an important role in maintenance of genetic stability. 相似文献
53.
HEI Zi-qing WU Wei-kang SUN Hui-lan HUANG He-qing TAN Hong-mei LUO Han-chuan 《园艺学报》2003,19(3):345-347
AIM:To observe the effects of Sini decoction on atherosclerosis(AS) and ceramide content of aorta in rabbits. METHODS:28 rabbits were randomly divided into three groups. Control group was fed with a normal diet; High cholesterol group was fed 1% cholesterol and 5% fat diet; Sini decotion+ high cholesterol group was fed 1% cholesterol and 5% fat diet plus Sini decotion (4.2 g·kg-1·d-1). At the end of study, the plaque area were measured, the atorta ceramide and cell apoptosis were also detected. RESULTS:Sini decotion diminished lipid plaque area on the aortic endothelium, reduced the levels of aorta ceramide and the apoptosis index. CONCLUSION:Sini decoction has an inhibitory effect on AS, the mechanism may be that Sini decotion reduces concentration of ceramide in aorta. 相似文献
54.
菟丝子致死薇甘菊 总被引:10,自引:0,他引:10
薇甘菊Mikania micrantha是入侵的害草,已经由珠江三角洲扩散到粤东、粤西沿海地区,以及部分山区。在薇甘菊的天敌调查中看到菟丝子寄生于薇甘菊,严重时可以把薇甘菊致死。我们在本所的实验园中栽种薇甘菊,并让其被菟丝子Cusuta chinensis Lam.寄生,结果证实菟丝子在两个月左右,完全可以抑制薇甘菊的生长,最终把薇甘菊致死。这可能是以草治草的一种新途径,但是菟丝子也是我国农作物的一种重要昌,如何利用菟丝子控制薇甘菊的为害,而又不使菟丝子对薇甘菊的伴生植物和鞭它农作物造成为害,是下一步要解决的问题,菟丝子致死薇甘菊的机理更需要深入研究。 相似文献
55.
56.
57.
AIM: To prepare gfp-bcl-XL-contained recombinant adenovirus(rAd-gfp-bcl-XL).METHODS: Bcl-XL gene was amplified from pEGFP-C3-bcl-XL, subcloned into shuttle plasmid and formed transfer plasmid of pAdTrack-CMV-bcl-XL. Then pAdTrack-CMV-bcl-XL was linealinzed with PmeI and co-transformed into BJ5183 bacteria with adenovirus genomic plasmid of pAdEasy-1. The identified recombinant adenovirus plasmid was digested with PacI and transfected into 293 cells to package recombinant adenovirus particles. The target gene was detected by PCR.RESULTS: There were about 35% positive recombinant bacterial clones after the co-transformation of pAdTrack-CMV-bcl-XL and pAdEasy-1 into BJ5183. Recombinant adenovirus particle were produced and further amplified after the transfection of pAdEasy-1-gfp-bcl-XL into 293 cells. PCR test indicated that the recombinant Ad contained bcl-XL gene. The titer of the purified rAd-gfp-bcl-XL was 6.5×1012 PFU/L. CONCLUSIONS: The homologous recombination in bacteria is a convenient and high efficient method to prepare rAd-gfp-bcl-XL. This affords a good gene transfer vector for the gene therapy in human’s diseases. 相似文献
58.
AIM: To investigate the changes of myocardium glycogen content and the relation ship between changes of the myocardial glycogen content and the myocardial function. METHODS: Male Wistar rats were placed in (hypoxia rats) and made to swim in (hypoxia swimming rats) a hypobaxic chamber simulating an altitude of 5 000 m above sea level. The content of myocardium glycogens was determined by colorimetry. RESULTS: The myocardium
glycogen content of rats significantly reduced along with the prolongation of hypoxic exposure and approached to control level in hypoxia swimming rats. The myocardial function of right ventricule was improved significantly compared with control group.CONCLUSION: Moderate exercise (swimming) is beneficial to hypoxic adaptation of rats under the condition of chronic hypoxia. 相似文献
59.
60.