谷氨酰胺对肉仔鸡脾组织结构发育的影响

160只 1日龄艾维茵肉仔鸡随机分成 4组 ,分别饲喂添加 0 % ,0 2 % ,0 4%和 0 8%谷氨酰胺的饲粮 2 8d。每周末每组取 6只鸡 ,颈静脉放血致死 ,取脾脏 ,Bouin液固定 ,制作石蜡切片 ,HE染色 ,光镜观察 ,显微摄影 ,研究基础日粮中添加谷氨酰胺对肉仔鸡脾组织结构的影响。结果显示 :添加谷氨酰胺的各试验组脾小结增多、增大 ;动脉周围淋巴鞘增厚 ;椭球增多、增大 ,细胞排列疏松 ,鞘毛细血管内皮细胞增高、增多 ;红髓比例降低 ,脾索内浆细胞和巨噬细胞增多。添加 0 8%谷氨酰胺的脾小结变化明显 ,添加 0 4%谷氨酰胺的动脉周围淋巴鞘和椭球变化明显。说明日粮中适量添加谷氨酰胺可促进脾内淋巴细胞的增殖与分化 ,从组织学角度证明 ,谷氨酰胺可促进脾的免疫应答能力 ,提高机体的免疫功能     

温度对杨树菇栽培性状影响的统计学分析

分析了温度环境因子对杨树菇生长发育的影响 ,研究结果表明 :菌丝体营养生长以 2 5～ 2 7℃为最适。原基分化温度 11～ 16℃ ,子实体发育以 13～ 2 6℃最适 ,不同菌株的最适温度存在差异。通过对日平均气温与杨树菇生产周期和产量、品质的关系试验 ,温度与病害发生关系试验 ,用统计分析方法得出了杨树菇各生理阶段的最适温度范围 ,为杨树菇的优质高产栽培提供了温度环境因子的基础数据和调控依据。     

桃树根癌病防治技术探讨

桃树根癌病是公认最难防治的土传细菌病害之一。在对该病进行较系统研究的基础上,采用多种抗菌药剂、多种方法进行了室内、外药敏测定与防治试验,显示出异菌氰、次氯酸钠、乙蒜素、妥布霉素、环丙沙星、庆大霉素及其混用对防治桃树根癌病菌有明显防效。     

新疆盐碱地种植耐盐小麦土壤盐分的变化

新疆干旱区有大量盐碱地 ,很大程度上限制农业的可持续发展。盐碱的利用改良和水资源的高效利用的研究 ,面临巨大的挑战。盐碱地上种植耐盐小麦既有生产效益 ,又有改良土壤盐渍化作用。在传统灌溉管理下 ,当土壤原始盐分 2 .0 %时 ,在壤质土壤上种植耐盐小麦 1— 2年 0— 40cm土层盐分可降到 0 .8%。在粘壤土、壤土土质的土壤中 ,种植耐盐小麦 5年时 ,0— 1 0 0cm土层盐分可降到 0 .5 % ;种植 7年后 ,0— 1 0 0cm土层盐分降到 0 .2 %左右 ,且在以后种植条件下 ,土壤盐分趋于平衡状态 ,变化很小。种植耐盐小麦引起的盐分变化 ,灌溉水为主要作用 ,以至经多年种植 ,土壤表现为脱盐碱化现象。     

Roles of the Kv1.2, Kv1.5, Kv2.1 potassium channels in hypoxia pulmonary vasoconstriction

AIM: To determine the role of Kv1.2, Kv1.5, Kv2.1 in the hypoxia pulmonary vasoconstriction (HPV). METHODS: Male Wistar rats were divided into two groups: normoxic group and hypoxic group. The single smooth muscle cell was obtained from pulmonary artery of Wistar rats with acute enzymatic digestion method. The conventional whole-cell patch clamp technique was used to record the resting membrane potential (Em) and the potassium currents of voltage-gated potassium channel (IKv) in rat pulmonary arterial smooth muscle cells (PASMC). Intracellular application of Kv1.2/Kv1.5/Kv2.1 antibodies (1∶125) was conducted through the whole-cell patch clamp system. RESULTS: ① Em of PASMC was depolarized after 24 h hypoxia compared with that of control cells . IKv of PASMC was decreased after 24 h hypoxia, . ② The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies depolarized Em and inhibited IKv in PASMC from normoxic rat, whereas the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on them. ③ The mixture of Kv1.2/Kv1.5/Kv2.1 antibodies and the mixture of Kir2.1/Kir2.3/Kir4.1 antibodies had no effects on IKv and Em from rats hypoxic for 24 h. CONCLUSION: Kv1.2, Kv1.5, Kv2.1 might be oxygen sensitive potassium channels which mediated HPV.     

Astrocyte proliferation in the rat hippocampus after middle cerebral artery occlusion

AIM: To study rat astrocyte proliferation in ipsilateral hippocampus following focal cerebral ischemia. METHODS: Ischemia was induced by temporary middle cerebral artery occlusion (MCAO). In hippocampus of rats at 3, 7 and 30 days after MCAO, the numbers and anatomic distribution of glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. The protein expression of GFAP and proliferating cell nuclear antigen (PCNA) in the ipsilateral hippocampus were analyzed by Western blot analysis. RESULTS: Astrocytes appeared hypertrophic, with increased process thickness and numbers at 7 days after MCAO, and the highest density of astrocytes were seen at 30 days in the CA1, CA2 regions of the ipsilateral hippocampus. Western blot analysis revealed that GFAP levels were normal at 3 days, but increased by 7 days and remained elevation at 30 days. Western blot analysis of PCNA protein also revealed identified upregulation PCNA at 3 days after MCAO and the expression peaked at 7 days. CONCLUSION: This study demonstrates that focal cerebral ischemia in the rat results in a rapid response, a process often referred to as reactive astrogliosis or glial scarring, from resident astrocytes of the ipsilateral hippocampus to the side of ischemia.     

Effects of changing PKC activity on proliferation and telomerase expression in human hepatocellular carcinoma cells

AIM: To investigate whether protein kinase C (PKC) is involved in the proliferation and the telomerase expression in human hepatocellular carcinoma cells. METHODS: Human hepatocellular carcinoma cells (BEL-7402) were treated with exogenous phorbol-12-myristate-13-acetate (PMA, PKC activator) and staurosporine (SP, PKC inhibitor) for 48 hours. The techniques of cell culture and the telomeric repeat amplification protocol silver staining in combination with computer image scanning system in vitro were used to observe the variations of the growth and the telomerase expression. RESULTS: The proliferative potential of BEL-7402 cells was decreased by the action of PMA as well as SP, and the telomerase expression was also inhibited by PMA and SP. CONCLUSION: Our findings suggest that the proliferation of human hepatocellular carcinoma cells and the telomerase expression may be related to PKC.     

Influence of Sini decoction on the proliferation and apoptosis of artery smooth muscle cells after balloon injury

AIM: To investigate the influence of Sini decoction (SND) on the proliferation and apoptosis of rabbit abdominal aorta smooth muscle cells after ballon injury and discuss the effect of vascular smooth muscle cell's (VSMCs) proliferation and apoptosis in post-percutaneous coronary intervention (PCI) restenosis (RS) and the feasibility of SND preventing post-PCI RS. METHODS: The animal model of rabbit abdominal aorta ballon injury was set up and the therapertic group was treated with SND. The shape of proliferative and apoptotic cell were investigated by electron microscope. Immunohistochemistry staining was performed using α-actin,PCNA and Cyclin E monoclonal antibodies. In situ Cell Death Detection Kit was used to identify apoptotic cells. Abdomial aorta angiography was operated in the 84th day subgroup and the stenosis degree was evalued by quantitative angiographic analysis. RESULTS: As compared with the control group, the therapeutic group displayed a lower proliferative percentage and a higher apoptosic percentage (P<0.05). Moreover, the apoptosic peek time was on the 14th day after operation,which was longer than the control group. CONCLUSION: SND effectly inhibited the proliferation of VSMCs and iuduced apoptosis in VSMCs.     

Effects of Sini decoction on the ultrastructure of small intestinal epithelial cells in rats with intestinal ischemia-reperfusion

AIM: To investigate the effect of Sini decoction (SND)on the ultrastructure of small intestinal epithelial cells in rats with intestinal ischemia-reperfusion. METHODS: Thirty-two Sprague-Dawley rats of both sexes were randomly divided into 4 groups: (1) Control group in which sham operation was performed; (2) Model group in which intestinal I/R was produced by clamping super mesenteric artery(SMA) for 1 hour and declamping SMA for 3 hours; (3) SND1 group in which SD (0.6 g/200 g rat) was given via stomach tube 3 d before intestinal I/R; (4) SND2 group in which SD (1.2 g/200 g rat)was given via stomach tube 3 d before intestinal I/R. A strip of small intestine was taken from distal end of ileum for electron microscopic examination. The two-dimensional structural parameters and three-dimensional structural parameters of mitochondria were calculated. RESULTS: (1)Morphological changes of small intestine: In control group, epithelial cells were orderly arranged, with normal mitochondria and intestinal villi. In model group, the gaps between epithelial cells widened. There were a lot of apoptotic cells. Microvilli were short and swelled. Mitochondria were swelled obviously with broken ridges. Endoplasmatic reticulum was severely dilated. In SND1 and SND2 groups, microvilli and epithelial cells were orderly arranged relatively, mitochondria was slightly swelled. (2) Structural parameters of mitochondria: In model group, there were the least mitochondria and the swelling of mitochondria was severe. In SND1 and SND2 groups, the mitochondria was more than that of model group and the swelling were slight. CONCLUSION: Sini decoction can protect small intestine from ischemia-reperfusion injury without dose-dependent effect.     

体外培养条件对延边黄牛受精卵体外发育的影响

从延边黄牛卵巢中采集未成熟的卵母细胞进行体外成熟培养、体外受精及受精卵体外培养。结果表明 :1将卵子用 2种不同的培养液进行体外成熟培养和受精卵体外培养 ,TCM199组的卵裂率 (5 6 .3% )极显著高于 D- PBS组(33.6 % ) (P<0 .0 1) ;TCM199组的囊胚发育率和孵化率 (15 .1%、13.7% )虽高于 D- PBS组 (10 .5 %、8.7% ) ,但 2个组之间无显著差异 (P>0 .0 5 )。2以 TCM199作为基础培养液 ,分别用含激素培养液和不含激素培养液进行成熟培养和受精卵体外培养 ,添加激素组的卵裂率、囊胚发育率及囊胚孵化率 (81.2 %、17.5 %、15 .3% )高于没有添加激素的对照组 (75 .8%、12 .1%、10 .5 % ) ,但 2个组之间无显著差异 (P>0 .0 5 )。 3体外受精卵与单层颗粒细胞共培养组的卵裂率、囊胚发育率及囊胚孵化率 (78.0 %、11.5 %、9.9% )显著高于非共培养组 (6 8.1%、5 .4 %、3.6 % ) (P<0 .0 5 )。