Effect of inhaled nitric oxide on the gas exchange and hemodynamic of ischemia-reperfusion lung in rabbit model

AIM:To evaluate the effect of pulmonary gas exchang and hemodynamic by inhaled nitric oxide on ischemia-reperfusion(I/R) lung in rabbit model.METHODS:Forty Newzealand rabbits were divided into four groups: group Ⅰ, sham-operated; group Ⅱ, sham-operated with inhaled nitric oxide (NO); group Ⅲ, I/R; group Ⅳ, I/R with Inhaled NO. Pulmonary artery pressure (PA), pulmonary vascular resistance(PVR), arterial oxygen tension(PaO₂), arterial carbon dioxide tension (PaCO₂), alveolar-arterial gradient (A-aDO₂), Shunt fraction (Qs/Qt) expression of CD₁₈, peripheral leukocyte, methemoglobin were continuetly quantitated. RESULTS:At the start of reperfusion inhaled NO significantly improved PaO₂ and decreased PA, PVR,PaCO₂,A-aDO₂,Qs/Qt.CONCLUSION:Inhaled NO ameliorated significantly the early gas exchange and hemodynamic of reperfusion lung.     

Effects of oxidative stress and inflammatory response on kidney injury in mice with hyperthyroidism

AIM To explore the effects of oxidative stress and inflammatory response on kidney injury induced by hyperthyroidism in mice. METHODS Forty male Kunming mice were randomly divided into control group （n=20） and L-thyroxine （T4） group （n=20）. The mice in T4 group were intraperitoneally injected with T4 diluent at a dose of 1 mg/kg to induce hyperthyroidism, and those in control group were injected with normal saline of the same volume. After 7 weeks, the mice were weighed and dissected, the kidneys were removed and weighed, and the length of tibia was also measured. The activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in the kidney tissues were detected. The pathological changes of the kidney tissues were observed by HE staining. The levels of 4-hydroxynonenal （4-HNE）-modified proteins, interleukin-1 receptor-associated kinase 1 （IRAK1） and tumor necrosis factor receptor-related factor 6 （TRAF6） were determined by Western blot and immunohistochemistry. RESULTS Compared with control group, the body weight of the mice was decreased, while the kidney size and weight were increased significantly in T4 group. In addition, the ratios of kidney weight/body weight and kidney weight/tibia length were also increased （P<0.05）. In T4 group, the renal tubules were enlarged, and the epithelial cells of renal tubules were swollen and exfoliated, with vacuolar degeneration. Furthermore, reduced SOD activity, and increased MDA content and 4-HNE-modified proteins were found in T4 group, all of which were related to oxidative stress （P<0.05）. The levels of inflammation-related proteins IRAK1 and TRAF6 were significantly increased in T4 group （P<0.05）. CONCLUSION Excessive T4 may lead to kidney hypertrophy and injury in mice, and the mechanism may be related to oxidative stress and inflammatory response.     

草鱼三元病原菌融合子的构建及生物学特性

采用细胞融合技术首次将肠型点状产气单胞菌菌株和鱼害粘球菌菌株融合,构建二元融合菌株AM-1,再将AM-1与荧光假单胞菌菌株融合,构建三元融合菌株AM-2。对AM-1和AM-2菌株的形态结构、生理生化特性、致病性、抗原性、抗体效价进行分析。结果表明,AM-1活菌椭圆形,无鞭毛,死菌短杆状,AM-2菌株短杆状,有鞭毛,它们的生理生化性状介于3原始亲本菌株之间;用AM-2菌株对当年健康草鱼种进行人工感染攻毒,被攻毒草鱼种具有烂鳃病、赤皮病及肠炎病的部分症状;AM-2菌株抗原的受免草鱼种对荧光假单胞菌、鱼害粘球菌和肠型点状产气单胞菌菌株的免疫保护率分别达64.90％、68.00％、67.00%,抗体效价最高达1∶256、1∶256、1∶512。测定了AM-1和AM-2菌株及亲本DNA含量并进行了琼脂糖凝胶电泳分析,AM-1和AM-2菌株DNA含量明显高于任一原始亲本。图4表6参23     

EM浸种对玉米苗期农艺性状的影响

以玉米品种农大108为试验材料,研究不同浓度EM稀释液浸种对玉米幼苗生长发育的影响,遴选出玉米施用EM的最佳浓度。试验结果表明:EM浸种可促进玉米幼苗生长发育,增加玉米苗期株高和茎粗,增加玉米幼苗的叶面积,从而提高玉米幼苗素质,形成壮苗。500倍EM稀释液是玉米生产上较为理想的施用浓度。     

剑麻对重金属铅的吸收特性与累积规律初探

以热带地区一种生物量极大的经济作物剑麻(AgaveSisalanaPerrineexEngelm)作为研究对象,采用网室砂培,对Pb在该植物体内的吸收特性、累积分布及迁移规律进行了初步的探索。试验表明,Pb处理浓度低于1300mg·kg-1时对剑麻生长影响较小,但Pb加入浓度达到15900mg·kg-1时,显著抑制了剑麻的生长,其生物量仅为对照的6.7%。剑麻对Pb有很强的吸收性,并分布在其植株的各个部位,其中根系的吸收性最强,是地上部的3.0～13.6倍。Pb处理浓度为12700mg·kg-1时,剑麻地上部和根系的吸收量最大,分别为2220.3和22544.8mg·kg-1,是对照的65.6和221.3倍,且地上部迁移量高达55.87mg·株-1,说明剑麻对修复Pb污染土壤有一定的潜力,因而为今后探明植物积累Pb的机理和Pb污染土壤的植物修复提供了一种新的种质资源。     

土壤Cd处理对大豆叶片激素含量的影响

采用土壤盆栽试验与高效液相色谱法（HPLC）分析测试技术,研究了不同浓度Cd处理对大豆生长及叶片生长素（IAA）、赤霉素（GA3）、玉米素（Z）和脱落酸（ABA）含量变化的影响。结果表明,当Cd处理浓度≤0.50mg·kg^-1时,对大豆株高及幼苗期地上部生物产量无明显影响,Cd浓度≥2.50mg·kg^-1时,则表现明显的抑制作用。Cd浓度≥0.50mg·kg^-1时,对大豆地下部及花荚期地上部生物产量即表现明显的抑制作用。Cd胁迫抑制大豆叶片IAA和Z的合成,当Cd处理浓度为0.25mg·kg^-1时,大豆幼苗期和花荚期叶片IAA和Z含量分别为对照的43.1%、75.5%和60.6%、32.6%,Cd浓度升高,抑制作用加强。Cd胁迫对ABA的合成则表现明显的促进作用,当Cd处理浓度为2.50mg·kg^-1时,幼苗期和花荚期叶片ABA含量分别为对照的13.6倍和3.86倍。对GA3合成的影响则表现为低浓度下的促进作用和高浓度下的抑制作用,即当Cd处理浓度为0.25mg·kg^-1时,幼苗期和花荚期叶片GA3含量均高于对照,分别为对照的150.2%和109.7%,Cd浓度增至2.50mg·kg^-1时,其含量则分别为对照的36.4%和75.2%。大豆幼苗期和花荚期叶片ABA/IAA、ABA/GA3及ABA/Z与Cd处理浓度之间均达到了显著或极显著的相关性,表明Cd胁迫不仅影响植物激素的含量,同时也影响激素间的比例和平衡。     

基于GIS系统的土地利用数据建库模式研究

结合江苏省土地利用基础图件与数据更新调查,从技术路线、作业方法、数据库内容、数据库平台、数据组织、处理和存储等方面分别探讨了建设城乡一体化地籍数据库、建设大于1:1万比例尺的土地利用更新调查数据库及建设1:1万比例尺的土地利用现状数据库等3种基于GIS技术的土地利用数据建库模式;比较了3种模式的异同,并对建立土地利用数据库进行了较为深入的研究。     

11种观赏地被植物引种栽培和耐荫性试验

根据南京地区气候条件和绿化特点,结合种质资源调查,引种栽培了11种有观赏价值的地被植物;对荫蔽条件下引种材料的扩张能力和覆盖能力进行观察,证实:11种地被植物在75%和90%盖度的林下能正常生长发育,安全越冬、越夏,第2年春季恢复生长良好。采用分光光度法对3种不同处理条件下的11种地被植物叶绿素含量和叶绿素a/b进行测定,经90%遮光处理后,种间耐荫性强弱顺序为:佛甲草、青绿苔草、玉竹黄精、富贵竹、细叶麦冬、吉祥草、长春蔓、扶芳藤、连钱草、凹叶景天、无毛紫露草;75%遮光处理后种间耐荫性强弱顺序为:青绿苔草、玉竹黄精、凹叶景天、佛甲草、细叶麦冬、富贵竹、吉祥草、扶芳藤、长春蔓、连钱草、无毛紫露草。     

不同朝天椒种质资源品质和产量的综合评价

为了筛选适应贵州地区种植的朝天椒品种,以资源圃收集的48份朝天椒种质资源为研究对象,对其粗脂肪、蛋白质、维生素C、辣椒素、粗纤维、干物质等6项品质指标和产量进行测定,并利用隶属函数值法和聚类分析法对所得数据进行综合评价分析。结果表明：48份朝天椒种质资源的品质和产量存在较大差异。其中,粗脂肪含量范围在0.12~0.71 g/100 g之间,平均含量为0.47 g/100 g,品种‘黔辣10号’含量最高;蛋白质含量范围在2.36~3.67 g/100 g之间,平均含量为2.90 g/100 g,品种‘DZ-33’含量最高;维生素C含量范围在48.51~74.64 mg/100 g之间,平均含量为59.07 mg/100 g,品种‘子弹头’含量最高;辣椒素含量范围在6.63~72.54 mg/100 g之间,平均含量为31.49 mg/100 g,品种‘香脆白米椒’含量最高;粗纤维含量范围在2.32%~10.45%之间,平均含量为7.59%,品种‘黔辣18号’含量最高;干物质含量范围在11.12%~20.98%之间,平均含量为15.66%,品种‘单生理想’含量最高;干椒产量范围在189.66~442.81 kg/667 m²之间,平均产量为304.62 kg/667 m²,品种‘2018-70’平均干椒产量最高。6项品质指标的平均隶属度值在0.29~0.75之间,相差0.46,种质资源间差异较大。聚类分析可将48份朝天椒种质资源分为3大类群,第Ⅰ类群包括26份种质资源,占总资源数的54.17%,6项品质指标的平均含量和平均干椒产量都处于居中水平;第Ⅱ类群包括16份种质资源,占总资源数的33.33%,6项品质指标的平均含量都处于最低水平,但平均干椒产量处于最高水平;第Ⅲ类群包括6份种质资源,占总资源数的12.50%,6项品质指标的平均含量都处于最高水平,综合品质表现最好,但平均干椒产量最低。开展朝天椒种质资源品质和产量的综合评价分析,可为朝天椒种质资源的创新利用以及新品种选育奠定基础。     

印楝种籽初制工艺技术装备的研制

本文对印楝种籽的加工机理进行了研究,提出了满足生产需要的工艺路线,研制出了差速螺旋齿双级对滚破壳设备、吸风分离设备,获得了较好的加工效果。