Effect of Weaning Stress on Blood Physiology and Biochemistry Indexes and Immune Function in Dairy Buffalo Calves

To study the effect of weaning stress on dairy buffalo calves,5 healthy calves were chosen and weaned,and the blood samples were collected at 1 day before weaning,and 1,14 and 21 days after weaning to analysis blood physiology and biochemistry indexes and immune function.The results showed that the count of white blood cell,lymphocyte,erythrocyte,monocytes and hematocrit,aspartate aminotransferase activity,blood glucose,cortisol and immunoglobulin G contents of dairy buffalo calves were no significant difference between before and after weaning treatments (P > 0.05).The count of neutrophils at 21 d after weaning were significantly lower than that of 1 d before weaning of dairy buffalo calves (P < 0.05).However,lactate dehydrogenase and alanine aminotransferase activity in weaned treatments were significantly higher than those of animals in the treatment before weaning (P < 0.05).The serum urea nitrogen of dairy buffalo calves at 21 d after weaning was significantly higher than that of 1 d before weaning (P < 0.05) and hemoglobin content at 21 d after weaning was significantly lower than that at 1 day before weaning (P < 0.05).It indicated that the physiological and immunity function of dairy buffalo calves were challenged under weaning stress.     

Study on Clinical Efficacy of Oligosaccharide Sulfate and Dextran Sulfate Against Bovine Mastitis

In order to detect the clinical efficacy of oligosaccharide sulfate(DEAE) and dextran sulfate (DS-4000) against bovine mastitis,56 cows with bovine mastitis were divided into 5 groups (DEAE group,DS-4000 group,DEAE＋penicillin group,DS-4000＋penicillin group and penicillin group),and their breasts were perfusion with different drugs twice one day for 3 days.The effective rates of drugs were observed by clinic symptoms.Milk samples were collected before and after treatment of drugs,and the bacterial negative conversion rate of each group were detected by microbial examination.The results showed that total effective rates of 5 groups were 10.00%,20.00%,58.33%,75.00% and 50.00%,respectively,and the bacterial negative conversion rates were 0,12.50%,50.00%,54.55% and 44.44%,respectively.In conclusion,the curative efficiencies of the polysaccharides against bovine mastitis were weak,but it could be improved by combination with penicillin.     

Effects of lncRNA-GTL2 Transgenosis on Gut Microbiota Composition in Mice

This research was aimed to study whether the lncRNA would have effects on the structure and constitution of the intestinal microbial colonies in mice.High throughout sequencing was used to sequence and analyze the intestinal microbial colonies in both transgenic and non-transgenic mice of three months old.We conducted comparison and t-test at the level of phylum and genus.The result showed that transferred into long non-coding RNA genes GTL2 (lncRNA-GTL2),the mouse intestinal microbial colony structure and composition had no significant differences in the overall,within the same gender,but there were individual differences.Therefore,this study didn't find that long non-coding RNA transfered had a significant impact on the intestinal microflorain the phylum and genus level.     

Research Progress on Action Mechanism of Animal Anti-parasitical Drugs

Animal anti-parasitical drugs are mainly used to prevent and control animal parasitosis, and also an effective way to protect the healthy development of animal husbandry and public health security.The efficacy of anti-parasitical drugs depends on the interaction between drug molecular and different target tissues and target cells of parasite or animal.Because of the variety of anti-parasitical drugs, the anti-parasitical mechanisms are complex.With the development of new anti-parasitical drugs and the depth of scientific research, especially the development of chemical synthesis and biological pharmaceutical technology, the variety and quantity of anti-parasitical drugs constantly increase.With the discovery of new drug structure and its action target, the study on the mechanism is constantly deep.The author mainly summarized the research progress on action mechanism of anti-parasitical drugs.     

Protective Effect of Sulforaphane on Reproductive Function of Male Mice with Cadmium Poisoning

The study was aimed to explore the protective effect of sulforaphane (SFN) on the reproductive function of male mice with cadmium poisoning.40 healthy clean grade male Kunming mice were randomly divided into four groups:control group (H₂O),cadmium chloride group (2.3 mg/kg CdCl₂),sulforaphane group (10 mg/kg SFN),sulforaphane + cadmium chloride group (10 mg/kg SFN+2.3 mg/kg CdCl₂),and continuous administration for 10 d,all mice were executed by dislocated cervical vertebra at 2 d after the last administration,and then the pathologic changes of testicular tissues,organ coefficient of testicle and epididymis,sperm quality and concentration of testosterone were tested.Additionally,the contents of GSH and MDA,and the activities of T-SOD in testis were also detected at the same time. Compared with the control group,pathology damages were observed in cadmium chloride group,organ coefficient of testis and epididymis,sperm quality and levels of testosterone extremely significantly decreased (P<0.01),the activities of T-SOD and GSH content were extremely significantly decreased (P<0.01),and the concentration of MDA was extremely significantly enhanced (P<0.01).Compared with the control group,the activity of T-SOD and concentration of GSH in sulforaphane group were significantly increased (P<0.05),and the concentration of MDA was not significant different between the control group and sulforaphane group (P>0.05).While compared with the cadmium chloride group,the sperm motility rate and sperm total count in sulforaphane and cadmium chloride group were extremely significantly increased (P<0.01),the organ coefficient of testicle and epididymis was increased significantly (P<0.05),the concentration of GSH and activity of T-SOD in testicular tissue were extremely significantly increased (P<0.01),and the concentration of MDA was extremely significantly decreased (P<0.01).The results indicated that sulforaphane had the protection effect on reproduction function of male mice with cadmium poisoning.     

Effect of Vitamin E on Bovine Granulosa Cells Apoptosis and Proliferation through Cx43

The aim of this study was to determine the effect of vitamin E on Cx43,the mechanism and function of vitamin E on bovine granulosa cells apoptosis and proliferation.In this study,granulosa cells were isolated from bovine ovary and cultivated in vitro by adding different concentration of vitamin E (0,25,50,100,200 and 500 μmol/L) for 24 h.After cultured,apoptotic cells were detected by FCM,mRNA expression levels of BCL2/BAX、P53 and Cx43 genes were determined by Real-time PCR and cell proliferation was detected by CCK8.The results showed that compared to control group,100 μmol/L vitamin E could significantly inhibit the apoptosis of granulosa cells (P<0.05).Real-time PCR detection results showed that vitamin E significantly changed the mRNA expression levels of BCL2/BAX,P53,Cx43 genes (P<0.05).Vitamin E could significantly improve granulosa cells proliferation when granulosa cells were treated for 24 and 36 h (P<0.05).The results provided a theoretical basis on further analysis for studing the influence mechanism of vitamin E on oocytes development and maturity,and improvement of female animal reproduction by influencing granulosa cells proliferation and apoptosis.     

Study on Fans Cooling of Dairy Cowshed with Free Stall in Northern Mountainous Region of Hebei in Summer

The present objective of the study was to investigate the effect of fans cooling on dairy cow in northern mountainous region of Hebei in summer.Two cowsheds with the same structure and feeding technics were used to investigate the effect of fans on indoor temperature and humidity, physiological parameters and milking performance of dairy cow.The results showed that the average ambient temperature in cowshed with fans was 1.5℃ lower than that of the control cowshed, and the average wind velocity was 0.49 m/s higher than that of the control cowshed, reaching to 0.66 m/s.Temperature-humidity index (THI) of cowshed with fans was significantly lower than that of the control shed during the period of fans running (P<0.05).Besides, the rectal temperature and respiration rate of dairy cows in cowshed with fans were significantly lower than of the control cowshed (P<0.05), and the milk yield per cow per day was 17.0% higher than that of the control cowshed (P<0.05), however, the milk fat, milk protein and milk sugar were no significant difference from the control (P>0.05).Overall, fans could decrease indoor environment, moderate heat stress and increase performance and health of dairy cows in northern mountainous region of Hebei.     

Research Advanced on Functions of Nutritional Regulation and Intestinal Barrier of Arginine

Arginine is one of the most important amino acids for animals,and participates in the synthesis and metabolism of many nutrients,plays a role in the development and function of the gastrointestinal tract.Intestinal barrier function is one of the most functions for intestine.The article will summarize the effect of arginine on intestinal barrier.It was explicated the influence on inteseinal immunity of Toll-like receptor.This research provided some references for further study of arginine regulation on animal production.     

6种灌木不同器官生物量及其碳、氮含量的分配特征

通过研究晋北地区草地黄刺玫、沙棘、荆条、酸枣、绣线菊和柠条等6种常见灌木的叶片、新生枝、老龄枝生物量及其碳、氮含量的分配特征,旨在为草地准确估算草地植物碳、氮循环提供依据。结果表明:黄刺玫的地上生物量最大,是酸枣的7倍。沙棘叶片生物量占地上生物量的比例最大;黄刺玫新生枝生物量占地上生物量的比例最大;柠条老龄枝生物量占地上生物量的比例最大。黄刺玫、沙棘和荆条的地上部分各器官生物量分配特征相似,由高到低表现为叶片、老龄枝、新生枝;绣线菊、酸枣和柠条的地上部分各器官生物量分配特征相似,由高到低表现为老龄枝、叶片、新生枝。6种灌木地上部分各器官碳含量差异不显著,氮含量分布由高到低为叶片、新生枝、老龄枝。