圆点印迹法检测葡萄斑点病毒技术研究

采用圆点印迹法检测GFkV，结果表明：Tris-HCl和PBS在提取GFkV时效果相同，在带毒葡萄植株中韧皮部的GFkV含量较高。     

沙坡头地区土壤结皮形成机理的研究

本文通过大量的事实和依据论述了结皮形成的物质来源和结皮的形成与土壤性质的关系，表明了粉粒（０．０５－０．０１ｍｍ）含量是结皮形成的关键，并从结皮的发育过程、剖面变化、地理位置和养分含量等方面说明了粉粒的分选和聚积过程，进而论述了粉粒的累积机理、结皮的形成过程及结皮形成－破碎的动态变化。     

Semi-Synthesis,Cytotoxic Evaluation,and Structure—Activity Relationships of Brefeldin A Derivatives with Antileukemia Activity

Brefeldin A (1), a potent cytotoxic natural macrolactone, was produced by the marine fungus Penicillium sp. (HS-N-29) from the medicinal mangrove Acanthus ilicifolius. Series of its ester derivatives 2–16 were designed and semi-synthesized, and their structures were characterized by spectroscopic methods. Their cytotoxic activities were evaluated against human chronic myelogenous leukemia K562 cell line in vitro, and the preliminary structure–activity relationships revealed that the hydroxy group played an important role. Moreover, the monoester derivatives exhibited stronger cytotoxic activity than the diester derivatives. Among them, brefeldin A 7-O-2-chloro-4,5-difluorobenzoate (7) exhibited the strongest inhibitory effect on the proliferation of K562 cells with an IC₅₀ value of 0.84 µM. Further evaluations indicated that 7 induced cell cycle arrest, stimulated cell apoptosis, inhibited phosphorylation of BCR-ABL, and thereby inactivated its downstream AKT signaling pathway. The expression of downstream signaling molecules in the AKT pathway, including mTOR and p70S6K, was also attenuated after 7-treatment in a dose-dependent manner. Furthermore, molecular modeling of 7 docked into 1 binding site of an ARF1–GDP-GEF complex represented well-tolerance. Taken together, 7 had the potential to be served as an effective antileukemia agent or lead compound for further exploration.     

洋葱型大蒜及其成因分析

洋葱型大蒜为大蒜鳞茎异常生理变态所形成的类似洋葱鳞茎结构的大蒜(原称“面包蒜”),该鳞茎主要由肥厚的叶鞘基部及鳞芽的外层鳞片加厚所构成,无肉质鳞片或肉质鳞片极不发达,可形成蒜薹或无薹分化,无任何食用价值。洋葱型大蒜的形成与N、P、K超量施用且配比不当,追N过多且时间过早,土壤过于粘重并长期处于水分饱和状态等有密切关系。     

阿月浑子砧木研究进展

综合介绍了世界阿月浑子砧木资源、自然分布、栽培表现、育苗及繁殖技术及其研究进展。     

Isolation and structure of higher diamondoids,nanometer-sized diamond molecules

We exploited the exceptional thermal stability and diverse molecular shapes of higher diamondoids (C22 and higher polymantanes) to isolate them from petroleum. Molecules containing 4 to 11 diamond-crystal cages were isolated and crystallized, and we obtained single-crystal x-ray structures for representatives from three families. Rigidity, strength, remarkable assortments of three- dimensional shapes, including resolvable chiral forms, and multiple, readily derivatizable attachment sites make them valuable nanometer-size molecular building blocks.     

雨生红球藻规模化培养工艺的构建与应用

针对雨生红球藻规模化培养中的工艺流程、生物污染、温度控制、溶氧解析和pH值稳定等问题,于2003年到2005年,在山东省潍坊地区,开展了雨生红球藻中试规模的论证研究。建立了可用于雨生红球藻规模化生产的3种不同类型的光生物反应器(柱式、管道、生物幕)和人工调节太阳能的温控车间,并构建了一套二步规模化培养工艺流程。结果表明,研究构建的培养模式降低了培养过程中的潜在风险,并大大提高了雨生红球藻虾青素含量。与传统培养方式相比,年培养天数大约提高了3倍。在每个批次培养中,细胞生长培养效率提高了至少1.75倍,虾青素累积培养所需时间缩短了40%,虾青素含量提高了4倍。总体来看,研究构建的新工艺比传统开放模式的生产效率有很大提高,全年共计提高了35倍。     

赖草联会消失突变体的细胞遗传学研究

对赖草（Ａｎｅｕｒｏｌｅｐｉｄｉｕｍｄａｓｙｓｔａｃｈｙｓ）自发联会消失突变体的研究结果表明：突变体在偶线期、终变期尚能正常配对．但从终变期开始，大部分“×”、“Ｖ”、“‖”状二价体提早解离成单价体，从而导致以后各时期持续紊乱。和正常株不同，突变体出现了一定频率的“中—后”期分裂相。这是联会消失突变体的减数分裂特征。突变体的减数分裂过程中出现更多的染色体结构变异，这说明联会消失突变和结构变异有相关的遗传背景。由于减数分裂行为十分紊乱而导致突变体的配子育性和结实率大大下降。     

不同贮藏条件鸡蛋的脂类过氧化作用

本文以ＴＢＡ光度法分析了不同贮藏条件下鸡蛋脂类的过氧化作用,并分析了温度、氧对过氧化作用的影响。结果表明,在贮藏条件下,鸡蛋蛋黄中伴有明显的脂类过氧化过程。脂类过氧化物（ＬＰＯ）的水平随贮藏时间的延长而不断增加（Ｐ＜０．０１）,但蛋清中的ＬＰＯ在贮藏过程中没有明显的变化。低温和缺氧可明显抑制鸡蛋脂类的过氧化作用（Ｐ＜０．０１）。