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排序方式: 共有271条查询结果,搜索用时 15 毫秒
31.
Duangjai Pisuttharachai Motoshige Yasuike Hideaki Aono Keisuke Murakami Hidehiro Kondo Takashi Aoki Ikuo Hirono 《Fisheries Science》2009,75(1):195-206
Two complementary DNA (cDNA) libraries were constructed from phyllosomas and hemocytes of adult Japanese spiny lobster Panulirus japonicus and a total of 2,673 expressed sequence tags (ESTs) were obtained. After assembly and clustering, 450 and 458 unique sequences
were found from the phyllosoma and hemocyte cDNA libraries, respectively. Of these, 114 and 220 ESTs showed significant homologies
with known genes in the National Centre for Biotechnology Information (NCBI) database. The remaining sequences were of unknown
function. Immune-related genes found in this study include lectin, proteinase inhibitor, prophenoloxidase, heat-shock protein,
antimicrobial peptide, and a few putative defense-related proteins. 相似文献
32.
33.
Tamaki Masahiko Kobayashi Fumiyuki Suehiro Keisuke Ohsato Shuichi Sato Michio 《农业科学学报》2018,17(9):2024-2030
We investigated the possibility to reduce the usage of Blasin®Flowable (BF), a disinfectant inhibiting the germination and appressorium formation of Pyricularia oryzae Cavara conidia, by using carbon dioxide microbubbles (CO2MB). Germination was significantly inhibited by 10 000-fold diluted BF solution containing CO2MB generated by the decompression-type generator compared to CO2 millibubbles (CO2MMB) and CO2MB generated by the gas-water circulating-type generator. Appressorium formation in the 10 000-fold diluted BF solution containing both CO2MBs was less than that in CO2MMB. Scanning electron microscopy showed wrinkles and dents on the surface of conidia treated with 5 000-fold diluted BF solution containing both CO2MBs. Via transmission electron microscopy, we observed the expansion of the vacuole and the intracellular space and bloated or absent lipid granules in the conidia treated with BF solution containing both CO2MBs. Our results show that inhibition of the conidium germination and appressorium formation of P. oryzae Cavara by 10 000-fold diluted BF solution could be achieved by using the decompression-type CO2MB. 相似文献
34.
Yohei Kawasaki Yu Tanaka Keisuke Katsura Larry C. Purcell Tatsuhiko Shiraiwa 《Plant Production Science》2016,19(2):257-266
The difference in yields of cultivars may be causing difference in soybean yield between Japan and the USA. The objective of this study was to identify the effect of the cultivar on dry matter production and to reveal the key factors causing the differences in yield by focusing utilization of solar radiation in recent Japanese and US soybean cultivars. Field experiments were conducted during two seasons in Takatsuki, Japan (34°50′), and in a single season in Fayetteville (36°04′), AR, USA. Five Japanese and 10 US cultivars were observed under near-optimal conditions in order to achieve yields as close to their physiological potential as possible. The seed yield and total aboveground dry matter (TDM) were measured at maturity as long as radiation was intercepted by the canopy. The seed yield ranged from 3.10t ha?1 to 5.91t ha?1. Throughout the three environments, the seed yield of US cultivars was significantly higher than that of Japanese cultivars. The seed yield correlated with the TDM rather than the HI with correlation coefficients from .519 to .928 for the TDM vs. .175 to .800 for the HI, for each of the three environments. The higher TDM of US cultivars was caused by a higher radiation use efficiency rather than higher total intercepted radiation throughout the three environments. The seasonal change in the TDM observed in four cultivars indicated that dry matter productivity was different between cultivars, specifically during the seed-filling period. 相似文献
35.
36.
Imtiaj Hasan Shigeki Sugawara Yuki Fujii Yasuhiro Koide Daiki Terada Naoya Iimura Toshiyuki Fujiwara Keisuke G. Takahashi Nobuhiko Kojima Sultana Rajia Sarkar M. A. Kawsar Robert A. Kanaly Hideho Uchiyama Masahiro Hosono Yukiko Ogawa Hideaki Fujita Jiharu Hamako Taei Matsui Yasuhiro Ozeki 《Marine drugs》2015,13(12):7377-7389
MytiLec; a novel lectin isolated from the Mediterranean mussel (Mytilus galloprovincialis); shows strong binding affinity to globotriose (Gb3: Galα1-4Galβ1-4Glc). MytiLec revealed β-trefoil folding as also found in the ricin B-subunit type (R-type) lectin family, although the amino acid sequences were quite different. Classification of R-type lectin family members therefore needs to be based on conformation as well as on primary structure. MytiLec specifically killed Burkitt''s lymphoma Ramos cells, which express Gb3. Fluorescein-labeling assay revealed that MytiLec was incorporated inside the cells. MytiLec treatment of Ramos cells resulted in activation of both classical MAPK/ extracellular signal-regulated kinase and extracellular signal-regulated kinase (MEK-ERK) and stress-activated (p38 kinase and JNK) Mitogen-activated protein kinases (MAPK) pathways. In the cells, MytiLec treatment triggered expression of tumor necrosis factor (TNF)-α (a ligand of death receptor-dependent apoptosis) and activation of mitochondria-controlling caspase-9 (initiator caspase) and caspase-3 (activator caspase). Experiments using the specific MEK inhibitor U0126 showed that MytiLec-induced phosphorylation of the MEK-ERK pathway up-regulated expression of the cyclin-dependent kinase inhibitor p21, leading to cell cycle arrest and TNF-α production. Activation of caspase-3 by MytiLec appeared to be regulated by multiple different pathways. Our findings, taken together, indicate that the novel R-type lectin MytiLec initiates programmed cell death of Burkitt’s lymphoma cells through multiple pathways (MAPK cascade, death receptor signaling; caspase activation) based on interaction of the lectin with Gb3-containing glycosphingolipid-enriched microdomains on the cell surface. 相似文献
37.
Sukata T Uwagawa S Ozaki K Sumida K Kushida M Kakehashi A Wanibuchi H Miyata K Ogata K Fukushima S 《Journal of toxicologic pathology》2009,22(4):281-288
Previously, we reported α(2)-macroglobulin (α(2)M) to be a novel marker characteristic of rat hepatocellular preneoplastic and neoplastic lesions negative for hitherto well-established markers. In the present study, we further examined other candidate markers with specificity for the same type of lesions. Glutathione S-transferase-placental form (GST-P)-negative hepatocellular altered foci (HAF) were generated using a two-stage (initiation and promotion) carcinogenesis protocol with N,N-diethylnitrosamine (DEN) and either Wy-14,643 or clofibrate, two peroxisome proliferators. Microarray analysis using total RNAs isolated from laser-microdissected GST-P-negative HAF (amphophilic cell foci) and adjacent normal tissues was conducted along with immunohistochemistry and real-time RT-PCR. Staining for glucose-regulated protein 78 (GRP78) was detected in GST-P-negative HAF and hepatocellular adenomas, and slightly increased GRP78 mRNA expression was observed in the lesions by real-time RT-PCR analysis. Thus, an early increase of GRP78 expression in hepatocarcinogenesis is likely a feature of the amphophilic subset of HAF. 相似文献
38.
Yuka Miyoshi Ai Sakae Nao Arimura Keisuke Kojiro Yuzo Furuta 《Journal of Wood Science》2018,64(2):157-163
In this study, to summarize the changes of thermal-softening behaviors of wood and acetylated wood due to differences in the kinds of swelling liquids, the following measurements were conducted. Untreated and acetylated wood samples were swollen by various liquids and the temperature dependences of the dynamic viscoelastic properties were measured after the heating and cooling histories were unified among the samples. The results obtained are as follows. Untreated samples swollen by high-polarity liquid had lower peak temperature of tanδ, however acetylated samples had higher peak temperature of tanδ than those of untreated wood. On the other hand, untreated wood samples swollen by low-polarity liquid had higher peak temperature of tanδ, however acetylated samples had lower peak temperature of tanδ than those of untreated wood. The amount of swelling is determined by interaction between wood and liquid due to proton-accepting power and molar volumes of liquid and so on, therefore the peak temperature of tanδ and degree of reduction in dynamic elastic modulus (E´) with increasing temperature were corresponded to the amount of swelling. 相似文献
39.
Keisuke Kikuchi Rie Yamashita Satoshi Sakuragawa Keigo Hasumi Yasuhiro Mukai Hiroya Kobayakawa Shojiro Wakabayashi Yukie Saito 《Journal of Wood Science》2018,64(5):642-649
Bamboo-derived activated carbon prepared by superheated steam (BAC) exhibited performance for utilization as an electric double layer capacitor (EDLC) electrode. Pore structure and EDLC performances were investigated by comparison with phenol resin-derived activated carbon (MSP-20), which is commercially available and often used for the purpose. The nitrogen adsorption isotherm showed that BAC had a large BET-specific surface area of 1268 g/m2 with a developed pore structure, especially of the mesopore, in comparison with MSP-20. It is considered that inherent ash in bamboo promoted activation, in addition to physical activation by superheated steam. Capacitance per electrode volume (CV) was 52 F/cm3 with BAC. Because the density of BAC is high (0.78 g/cm3) compared with that of MSP-20 (0.58 g/cm3), sufficient CV for usage was obtained, although the capacitance per electrode mass (CM) at 5 mA/cm2 itself of BAC (67 F/g) was lower than that of MSP-20 (126 F/g). With IR drop, the resistance value of BAC (9.7 Ω) was lower than that of MSP-20 (10.5 Ω). Especially, the diffusion resistance of BAC disclosed to be smaller than that of MSP-20. These results indicated that BAC produced by steam activation is a promising material with a pore structure suitable for ion transfer in EDLC. 相似文献
40.
This study is focused on what factor mainly affects the mechanical properties of each wood species in the lateral direction. At first, the influence of the density which is closely related to mechanical properties in the longitudinal direction was also researched in the lateral direction. Thus, the elastic modulus, strength, and failure strain in the lateral tension were measured using thin cross-sectional samples of softwoods and hardwoods, having wide varieties in the density and anatomical features. The results obtained are as follows. The linear relationship between the density and the elastic modulus which has been verified in the longitudinal direction was not observed in the lateral tension for the samples with the annual ring inclination of 90°, which samples were influenced by ray arranged parallel to the tensile direction. However, samples with the annual ring inclination of 45° showed the high correlation between the density and the elastic modulus due to the shearing deformation of the cell shape. On the other hand, the proportional relationship between the elastic modulus and strength which has been verified in the longitudinal direction was not observed in the lateral tension except for the samples with the annual ring inclination of 90°. From the results obtained, it was revealed that the mechanical properties of wood in the lateral direction were significantly affected not only by the density but also by the structural features such as deformation of cell shapes, arrangement of ray or vessels, and the degree of the transition from the earlywood to the latewood. 相似文献