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41.
42.
Effects of Chinese herbal medicine and cold exposure on plasma glucose,leucine and energy metabolism in sheep
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X. Liang J. Jin X. Bi M. Kamruzzaman T. Kudo H. Sano 《Journal of animal physiology and animal nutrition》2018,102(2):e534-e541
An experiment was carried out to assess the feeding effect of Chinese herbal medicine on N balance, ruminal fermentation characteristics, kinetics of plasma glucose, leucine and energy metabolism in sheep kept at thermoneutral environment (23°C) or exposed to cold (2–4°C). Four sheep were subjected to either mixed hay (MH‐diet) or hay supplemented with 2% of a traditional nourishing Chinese herbal medicine mixture (Astragalus root, Angelica root and Atractylodes rhizome; CHM‐diet) over two 23‐day periods using a crossover design. Cold exposure was conducted for 5 days. The isotope dilution of [U‐13C]glucose with open circuit calorimetry was used to determine the turnover and oxidation rates of plasma glucose and metabolic heat production. The rate of plasma leucine turnover was measured with an isotope dilution method using [1‐13C]leucine. N intake was higher, N excretion through faeces was lower and N digestibility was higher for the CHM‐diet than the MH‐diet. Rumen pH was lower, concentration of rumen NH3 was higher, concentrations of rumen total VFA and acetate tended to be higher and propionate was higher for the CHM‐diet compared with the MH‐diet. Turnover rate of plasma glucose was higher for the CHM‐diet than the MH‐diet and increased during cold exposure. Oxidation rate of plasma glucose did not differ between diets and also between environments. Turnover rate of plasma leucine was higher for the CHM‐diet compared with the MH‐diet but remained similar between environments. Heat production was greater for the CHM‐diet than the MH‐diet and increased during cold exposure. No significant diet × environment interaction was detected. The present results demonstrated that plasma glucose and energy metabolism were enhanced by both Chinese herbal medicine and cold exposure; plasma leucine metabolism was enhanced by Chinese herbal medicine but with lack of change in response to cold exposure in sheep under the conditions of the current experiment. 相似文献
43.
44.
Hirano M Watanabe K Murakami M Kano R Yanai T Yamazoe K Fukata T Kudo T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(3):283-284
We excised surgically a feline granulomatous lesion and performed histopathological, mycological and molecular examinations. As a result, it was diagnosed as sporotrichosis, which was the second recorded case of a cat so afflicted in Japan. After the operation, we recognized another nodule on the lymph node. Histopathological examination was therefore performed, but no fungi were detected. To prevent recurrence, the cat was administered a antimycotic drug, itraconazole. As a result, no recurrence was found. Excision of the lesion is the treatment of choice for feline sporotrichosis. 相似文献
45.
de Cocq P Clayton HM Terada K Muller M van Leeuwen JL 《Veterinary journal (London, England : 1997)》2009,181(3):266-273
Pressure measurement devices in equine sports have primarily focused on tack (saddle pads and saddle fitting methods). However, saddle pressure devices may also be useful in evaluating the interaction and distribution of normal forces between the horse and rider, including rider position and riding technique. This study examined the validity, reliability, repeatability and possibilities of using a saddle pressure device to evaluate rider position. All measurements were performed using a standing horse. Validity was tested by calculating the correlation coefficient between measured normal force and the weight of the rider. Repeatability was tested by calculating intra-class correlation coefficients. The use of normal force measurements to evaluate horse–rider interaction was tested by adding a known weight to saddle or rider and collecting measurements with the rider sitting in four different positions.The device was found to be valid and reliable for force measurements when the measurement device was not replaced. The system could be used to determine the expected differences with added weight and in different rider positions. The normal force distribution measurement device proved to be a valid and reliable tool for studying the interaction between a rider and a static horse provided it is positioned carefully and consistently relative to both the horse and the saddle. 相似文献
46.
In March 2006, stored fruits of the medium-to-late-ripening citrus variety Shiranuhi ([Citrus unshiu × C. sinensis] × C. reticulata) were found to have a disease similar to blue mold. The fungus causing this disease differed distinctly from the well-known,
blue mold agent, Penicillium italicum, because it formed whisker-like coremia measuring 1–8 mm. On the basis of the morphological characteristics and the phylogenetic
analysis based on the nucleotide sequence of the β-tubulin gene, the fungus was identified as P. ulaiense. This is the first report of citrus whisker mold caused by P. ulaiense in Japan. 相似文献
47.
Naruto?FuruyaEmail author Fumika?Shimokusuzono Yutaka?Nakamura Kishiko?Nishimura Minoru?Takeshita Nobuaki?Matsuyama Kayo?Manabe Youichi?Takanami 《Journal of General Plant Pathology》2004,70(1):39-44
Crown gall disease of tobacco was found in Iwate Prefecture, Japan in 1995. Ten bacterial isolates, obtained from the galls of tobacco, were identified as Agrobacterium tumefaciens (Smith and Townsend 1907) Conn 1942 biovar 1 based on their ability to induce galls on the 14 tested plants, including tobacco after needle-prick inoculation, and on 12 cultural, physiological, and biological characteristics. The growth of the causal organism was not inhibited in vitro by agrocin of A. radiobacter strain K84. This report is the first on the natural occurrence of crown gall caused by A. tumefaciens on tobacco plants. 相似文献
48.
Midori?Sugimoto Masanori?KoikeEmail author Hideyuki?Nagao Kayo?Okumura Masayuki?Tani Katsuhisa?Kuramochi 《Phytoparasitica》2003,31(5):450-457
Forty-three isolates ofVerticillium lecanii from insects, phytopathogenic fungi and other substrates were tested for vegetative compatibility by observing heterokaryon
formation among complementary nitrate-nonutilizing (nit) mutants.nit mutants were isolated from 42/43 strains examined. Twenty-one isolates were self-incompatible, and the remaining 21 isolates
were divided into 14 vegetative compatibility groups (VCGs): ten containing only a single strain each, and the remaining four
containing two to four isolates each. Members of isolates in each of these VCGs all shared the same IGS haplotype. Further,
the isolates within a VCG were correlated with one another in part by fragment patterns of mt-LrDNA, -SrDNA, Bt-2 and H4 region,
by PCR-RFLP and -SSCP, but not by dsRNA. Two isolates belonging to VL-J2 have high virulence to aphids, whereas strains from
VL-J1 lack this character. These findings indicate that two VCGs (VL-J1 and -J2) may originate from two distinct clonal lineages.
Alternatively, high VCG diversity and HSI frequency ofV. lecanii might be associated with an array of distinct lineages. These data not only suggest relationships among DNA polymorphisms,
virulence, and VCG, but also demonstrate genetic heterogeneity ofV. lecanii.
http://www.phytoparasitica.org posting Sept. 30, 2003. 相似文献
49.
Takiguchi M Watanabe T Okada H Kudo T Yamada K Yasuda J Hashimoto A 《The Journal of small animal practice》2002,43(6):269-271
A urinary bladder tumour was diagnosed in a two-year-old female Maltese with haematuria and pollakiuria on the basis of ultrasonography and pneumocystography findings. The mass was resected, and the bladder was preserved at surgery. Histological and immunohistochemical examination confirmed the tumour to be a rhabdomyosarcoma, which has rarely been reported in small breeds of dog. There was no recurrence of the tumour at the original site in the urinary bladder two months later, when the dog died due to metastasis to the liver. This is believed to be the first report of bladder rhabdomyosarcoma in a Maltese. 相似文献
50.
Kojima AY Kobayashi S Acosta TJ Kudo M Miyamoto A Takagi M Miyazawa K Sato K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(2):119-122
Microdialysis System (MDS) is a novel technique used for investigation of molecule secretion between different cell populations. Local hormonal secretion at follicular wall has been still unclear. This MDS study was used to determine progesterone (P4), androstenedione (A4), estradiol-17beta (E2) and Prostaglandin F2alpha (PGF2alpha) release in mare pre-ovulatory follicles. Follicles larger than 30 mm were isolated from the ovary and follicular fluid aspirated for hormone assay. Follicular fluid collected from small, middle and large follicles were analyzed by EIA. The concentrations of P4 and PGF2alpha were similar among the different sizes of follicles. The release of A4 was observed in middle and large follicles. E2 concentration was observed in middle follicles and was higher in large follicles compared with middle follicles. Follicular wall was cut and incubated for MDS and when LH was infused, there was an increase in P4 and A4 release. PGF2alpha release was considerably high after LH infusion compared to the control group. Infusion of PGF2alpha increased P4 and A4 release but there was no change in E2 release. This results suggest that in pre-ovulatory follicles, LH stimulates theca interna cells and also PGF2alpha seemed to have a mediator role to induce steroid hormone production and luteinization of follicular cells. The nature of the mechanisms involved in selection of large follicles is still a perplexing research problem in reproduction. 相似文献