Presence and impact of the exercise-induced collapse associated DNM1 mutation in Labrador retrievers and other breeds

The impact of the mutation causing dynamin 1 (DNM1)-associated exercise-induced collapse (d-EIC) was determined in a retrospective genetic survey. The frequency of DNM1 mutant allele carriers in Labrador retrievers from conformation show, field trial/hunt test, pet or service lines ranged from 17.9% to 38.0% and the frequency of homozygous mutant (EE genotype) individuals ranged from 1.8% to 13.6%; 83.6% of these EE Labradors were reported to have collapsed by 4 years of age. DNM1 mutation carriers and EE dogs with a collapse phenotype were also detected in Chesapeake Bay retrievers, Curly-coated retrievers, Boykin spaniels, Pembroke Welsh corgis and mixed breed dogs thought to be Labrador retriever crosses. The DNM1 mutation was not identified in Golden, Flat-coated, or Nova Scotia duck tolling retrievers, or 15 other non-retrieving breeds. Veterinarians and breeders should be aware that the DNM1 EE genotype is not completely penetrant and that d-EIC is a widespread health concern in several very popular breeds, as well as breeds whose genetic similarity to retrievers is not obvious.     

An empirical approach to target DNA quantification in environmental samples using real-time polymerase chain reactions

Recent advances in molecular techniques have allowed for the routine examination of nucleic acids in environmental samples. Although current methodologies are very sensitive, accurate target DNA quantification from environmental samples remains challenging. To facilitate high-throughput DNA quantification from environmental samples, we developed a novel DNA quantification method based on a non-linear curve-fitting approach to extract additional information from quantitative PCR amplification curves and used the fitted parameters to develop multiple regression standard equations for target DNA quantification. A 3-parameter sigmoidal function performed superior to a 4-parameter Weibull function for generating the multiple regression standard equations. In a verification experiment, target DNA was quantified in a series of ‘unknown’ samples in three soils using this approach and the results were compared to target DNA values determined using corrected and uncorrected Ct-based (threshold cycle) methods. For each method, the deviations from the expected target DNA content were determined. Results clearly showed that over all DNA concentrations, target DNA content determined by the non-linear curve-fitting method was more accurate and more precise than values predicted by all other methods. Analysis of variance conducted on the predicted DNA contents also revealed fewer statistical artifacts with the non-linear curve fitting method compared to the conventional Ct-based methods. The novel approach described here is accurate, inexpensive, and very amenable for automation and high-throughput applications.     

Influence of carrot psyllid (Trioza apicalis) feeding or exogenous limonene or methyl jasmonate treatment on composition of carrot (Daucus carota) leaf essential oil and headspace volatiles

The effect of carrot psyllid (Trioza apicalis F?rster) feeding and limonene and methyl jasmonate (MeJA) treatments on the essential oil composition and headspace volatiles of carrot (Daucus carota ssp. sativus), cvs. Parano and Splendid, leaves was studied. Carrot psyllid feeding significantly increased the concentrations of sabinene, beta-pinene, and limonene, whereas limonene treatment increased the concentration of (Z)-beta-ocimene in the leaves of both cultivars. The limonene treatment significantly increased the concentration of total phenolics in the leaves of both cultivars, and MeJA treatment increased phenolic concentration in the leaves of Parano. Exogenous limonene spray did not decrease the number of carrot psyllid eggs laid either 2 or 24 h after treatment. The results suggest that carrot psyllid feeding induces changes in the endogenous monoterpene pool in the carrot leaves. Limonene and MeJA treatments affect some induced defenses of the carrot, but the exogenous limonene spray is not an effective oviposition deterrent against carrot psyllid.     

Structural characteristics of a low Arctic tundra ecosystem and the retreat of the Arctic fox

We conducted a large-scale, campaign-based survey in Finnmark, northern Norway to evaluate the proposition that declining Arctic fox populations at the southern margin of the Arctic tundra biome result from fundamental changes in the state of the ecosystem due to climatic warming. We utilized the fact that the decline of the Arctic fox in Finnmark has been spatially heterogeneous by contrasting ecosystem state variables between regions and landscape areas (within regions) with and without recent Arctic fox breeding.Within the region of Varanger peninsula, which has the highest number of recorded dens and the most recent breeding records of Arctic fox, we found patterns largely consistent with a previously proposed climate-induced, bottom-up trophic cascade that may exclude the Arctic fox from tundra. Landscape areas surrounding dens without recent Arctic breeding were here more productive than areas with recent breeding in terms of biomass of palatable and climate sensitive plants, the number of insectivorous passerines and predatory skuas. Even the frequency of unspecified fox scats was the highest in landscape areas where arctic fox breeding has ceased, consistent with an invasion of the competitively dominant red fox. The comparisons made at the regional level were not consistent with the results within the Varanger region, possibly due to different causal factors or to deficiencies in Arctic fox monitoring at a large spatial scale. Thus long-term studies and adequate monitoring schemes with a large-scale design needs to be initiated to better elucidate the link between climate, food web dynamics and their relations to Arctic and red foxes.     

The Remobilization of Metals from Iron Oxides and Sediments by Metal-EDTA Complexes

Synthetic chelating agents such as EDTA form strong complexes with heavy metals and therefore have the potential to remobilize metals from sediments and aquifers. In natural waters EDTA is present almost exclusively in the form of metal-complexes. Therefore, remobilization of metals is always a metal-metal-EDTA exchange reaction. We have investigated, to our knowledge for the first time, the remobilization of metals from the surface of synthetic iron oxides and from a river sediment by different metal-EDTA complexes. The metals are exchanged as follows:MeEDTA_dissolved + Me^ast _adsorbed Me^astEDTA_dissolved + Me_adsorbed The order of the remobilization rate of Zn²⁺ from goethite is CaEDTA > Fe(III)EDTA, reflecting the slow exchange reaction of Fe(III)EDTA. For the remobilization of Pb²⁺ from goethite, the rate was found to be Fe(III)EDTA > CaEDTA > ZnEDTA. Here, Fe(III)EDTA has surprisingly the fastest exchange rate. Only very limited remobilization of Pb²⁺ is possible from hydrous ferric oxide at pH 8 due to the very strong adsorption of Pb²⁺. The order of remobilization of Zn²⁺ from a natural river sediment was found to be CaEDTA > CuEDTA > Fe(III)EDTA. The remobilization rate of Zn²⁺ with Fe(III)EDTA is only 12% of the rate with CaEDTA, illustrating the importance of EDTA speciation for assessing remobilization.     

Wood properties of Scots pines (Pinus sylvestris) grown at elevated temperature and carbon dioxide concentration

Impacts of elevated temperature and carbon dioxide concentration ([CO2]) on wood properties of 15-year-old Scots pines (Pinus sylvestris L.) grown under conditions of low nitrogen supply were investigated in open-top chambers. The treatments consisted of (i) ambient temperature and ambient [CO2] (AT+AC), (ii) ambient temperature and elevated [CO2] (AT+EC), (iii) elevated temperature and ambient [CO2] (ET+AC) and (iv) elevated temperature and elevated [CO2] (ET+EC). Wood properties analyzed for the years 1992-1994 included ring width, early- and latewood width and their proportions, intra-ring wood density (minimum, maximum and mean, as well as early- and latewood densities), mean fiber length and chemical composition of the wood (cellulose, hemicellulose, lignin and acetone extractive concentration). Absolute radial growth over the 3-year period was 54% greater in AT+EC trees and 30 and 25% greater in ET+AC and ET+EC trees, respectively, than in AT+AC trees. Neither elevated temperature nor elevated [CO2] had a statistically significant effect on ring width, early- and latewood widths or their proportions. Both latewood density and maximum intra-ring density were increased by elevated [CO2], whereas fiber length was increased by elevated temperature. Hemicellulose concentration decreased and lignin concentration increased significantly in response to elevated temperature. There were no statistically significant interaction effects of elevated temperature and elevated [CO2] on the wood properties, except on earlywood density.     

盐胁迫对扁桃砧木营养器官离子吸收的影响

温室条件下,用NaCl和Na2SO4处理石头扁桃、桃扁桃和毛桃,处理浓度为0,75,150,300和350 mmol/L,处理10,20 d分别取叶、根和茎,测定各器官中离子吸收与分布变化规律。研究结果表明,盐胁迫下,3种砧木根、茎、叶中Na ,Cl-,SO42-含量及Na /K 比均随盐浓度的增加和处理时间的延长而增加,K 含量变化规律不稳定,但桃扁桃根、茎和叶片中K 略高于其他两个品种;石头扁桃根、茎、叶片中Na /K 比值高于其它两个品种,桃扁桃中Na /K 比值最低。桃扁桃根部Na ,Cl-和SO42-含量高于石头扁桃和毛桃,而叶片和茎中Na ,Cl-和SO42-含量却低于石头扁桃和毛桃。桃扁桃根部能够积累较多的Na ,阻止和减少了Na 向地上部的运输,从而减轻了Na2SO4对它的伤害,进而表明了桃扁桃耐盐能力较强。     

Scintigraphic, radiographic, and thermographic appearance of the metacarpal and metatarsal regions of adult healthy horses treated with nonfocused extracorporeal shock wave therapy--a pilot study

Nonfocused extracorporeal shock wave therapy (ESWT) treatment protocol is commonly used in veterinary practice. This study investigated the effects of four nonfocused ESWT treatments, given 2 weeks apart, on bone radiopharmaceutical uptake and radiographic and thermographic appearance in the metacarpal and metatarsal regions in six adult untrained horses. There were no measurable treatment effects determined by thermography (daily), scintigraphy (at 2-week intervals), and radiography (before study initiation and at study completion) between treated and control limbs. It was concluded that no gross evidence of bone remodeling is detectable by conventional clinical assessment when nonfocused ESWT is applied to healthy equine metacarpal or metatarsal bone.     

Clinical characteristics and persistence of bovine mastitis caused by different species of coagulase-negative staphylococci identified with API or AFLP

The coagulase-negative staphylococcal species causing mastitis in lactating cattle were identified and possible differences in the clinical characteristics or persistence of mastitis caused by different CNS were evaluated. The effect of antimicrobial treatment was also assessed. In addition, AFLP-typing of CNS was compared with the phenotypic identification. A total of 133 clinical or subclinical quarter cases of intramammary infection caused by CNS from the practice area of the Ambulatory Clinic of the University of Helsinki were studied. Bacteriological diagnosis was based on biochemical (API) testing. Staphylococcus simulans (43.6%) followed by S. chromogenes (23.3%) were the most common CNS species isolated from the milk samples. Ninety-nine isolates were genotyped using AFLP-analysis. Only 75.0% of S. chromogenes and S. simulans isolates identified with API test were clustered with the type strains of these species. Approximately half of the mastitis cases were clinical, and in the majority clinical signs were mild. The severity and persistence of intramammary infection were unaffected by CNS species. Fifty-nine percent of the quarter cases were treated with antimicrobials, and the rest were left without treatment. Mastitis due to beta-lactamase-negative CNS was treated with penicillin G and that due to beta-lactamase-positive CNS with cloxacillin. Nineteen percent of the isolates were beta-lactamase-positive. The bacterial cure rate for quarters treated with antimicrobials was high, 85.9%, as opposed to only 45.5% for untreated quarters. Bacterial cure rates for the most common CNS species or AFLP clusters were not statistically different. Further studies on identification of CNS species are needed.