Adipsin and complement factor D activity: an immune-related defect in obesity

Adipsin is a serine protease that is secreted by adipocytes into the bloodstream; it is deficient in several animal models of obesity, representing a striking example of defective gene expression in this disorder. Recombinant mouse adipsin was purified and its biochemical and enzymatic properties were studied in order to elucidate the function of this protein. Activated adipsin has little or no proteolytic activity toward most substrates but has the same activity as human complement factor D, cleaving complement factor B when it is complexed with activated complement component C3. Like authentic factor D, adipsin can activate the alternative pathway of complement, resulting in red blood cell lysis. Decreased (58 to 80 percent) complement factor D activity, relative to lean controls, was observed as a common feature of several experimental models of obesity, including the ob/ob, db/db, and monosodium glutamate (MSG)-injected mouse and the fa/fa rat. These results suggest that adipsin and the alternative pathway of complement may play an unexpected but important role in the regulation of systemic energy balance in vivo.     

Optimizing Phosphorus Fertilizer Management in Potato Production

Management of fertilizer phosphorus (P) is a critical component of potato production systems as potato has a relatively high P requirement and inefficiently uses soil P. Phosphorus promotes rapid canopy development, root cell division, tuber set, and starch synthesis. Adequate P is essential for optimizing tuber yield, solids content, nutritional quality, and resistance to some diseases. Although soil test P is the primary tool for assessing P fertilizer needs, in some areas petiole P analysis has been successfully utilized to guide in-season P applications. Potato has been shown in some studies to respond to fertilizer P at soil test levels considered very high for most other crops (100+ mg kg^?1 Bray P₁ or Mehlich I or III and 20+ mg kg^?1 sodium bicarbonate) especially on medium- to finer-textured soils. Even on high-testing soils, fertilizer P rates for top yields sometimes exceed 150 kg P₂O₅ ha^?1. In addition, many states/provinces continue to recommend half or more of the amount of P in the harvested portion of the crop irrespective of soil test P level. In most situations, few differences are expected among fertilizer P sources; however, high rates of diammonium phosphate (DAP) or urea-phosphate (UAP) should not be band-applied in contact or near the seed piece. Most research determined that fertilizer P was most efficiently used when band-applied at planting (e.g., 5 cm to each side of the seed piece); however, some western USA work on high-pH soils showed increased yields and petiole P levels with preplant broadcast applications. In-season applications with the irrigation water can be successful when the potato roots are sufficiently close to the soil surface; however, most research indicates that P applications are more effective when applied at planting or early in the season. Potato fertilizer phosphorus best management practices include: (1) apply the fertilizer P rate calibrated for local soils; (2) band-apply fertilizer P at least 5 cm from the seed piece, especially on very sandy soils or where DAP or UAP are used; (3) use petiole P tests to determine the need for in-season applications; (4) account for all P sources applied, including animal manures; and (5) utilize the best soil conservation practices to reduce P losses to surface waters.     

Alteration of lymphocyte trafficking by sphingosine-1-phosphate receptor agonists

Blood lymphocyte numbers, essential for the development of efficient immune responses, are maintained by recirculation through secondary lymphoid organs. We show that lymphocyte trafficking is altered by the lysophospholipid sphingosine-1-phosphate (S1P) and by a phosphoryl metabolite of the immunosuppressive agent FTY720. Both species were high-affinity agonists of at least four of the five S1P receptors. These agonists produce lymphopenia in blood and thoracic duct lymph by sequestration of lymphocytes in lymph nodes, but not spleen. S1P receptor agonists induced emptying of lymphoid sinuses by retention of lymphocytes on the abluminal side of sinus-lining endothelium and inhibition of egress into lymph. Inhibition of lymphocyte recirculation by activation of S1P receptors may result in therapeutically useful immunosuppression.     

Niche specialization and functional traits regulate the rarity of charophytes in the Nordic countries

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A novel automated method for the adjustment of ionic metal concentrations in soil extracts

A novel method is proposed for correcting metal fraction concentrations remaining within the sediment containing the solid residue of the sequentially extracted fraction. An easy‐to‐use Excel spreadsheet was prepared to assist adjustment of concentration in each fraction and demonstrate the difference between adjusted and non‐adjusted metal concentration of the fraction. The demonstration of a calculation of the modified BCR protocol data showed that this difference may reach 10–15% of the result value. The spreadsheet is available to download at: http://departments.agri.huji.ac.il/zabam/Rosen_Chen_Fraction_Adjustment_Formulae.xls     

Hybridization probes for the detection and differentiation of two serotypes of porcine rotavirus

A dot hybridization assay was developed to detect and differentiate two serotypes of porcine rotavirus by hybridization of specific probes to rotavirus RNA dotted onto nylon membranes. The assay was conducted using radiolabeled probes prepared from cDNA clones of OSU (serotype 5) and Gottfried (serotype 4) rotavirus gene 9 segments. The probes were prepared by excision of full length cDNA copies of gene 9 inserts from recombinant plasmids followed by nick translation and 32P-dCTP labeling. Rotavirus RNA samples used in the assay were prepared from tissue culture, and intestinal contents from gnotobiotic and conventional pigs. Optimal conditions for hybridization consisted of 52 degrees C, 50% formamide, and 5 x SSC. Hybridization studies with the OSU and Gottfried gene 9 probes have shown them to be specific with only low levels of cross-reactivity with heterologous rotavirus preparations. Sensitivity studies have demonstrated the ability of the probes to detect at least 2 ng of rotavirus RNA. Further development and refinement of this technique may provide a useful tool for the detection and differentiation of porcine rotavirus serotypes.     

Ionic liquid-mediated selective conversion of CO₂ to CO at low overpotentials

Electroreduction of carbon dioxide (CO(2))--a key component of artificial photosynthesis--has largely been stymied by the impractically high overpotentials necessary to drive the process. We report an electrocatalytic system that reduces CO(2) to carbon monoxide (CO) at overpotentials below 0.2 volt. The system relies on an ionic liquid electrolyte to lower the energy of the (CO(2))(-) intermediate, most likely by complexation, and thereby lower the initial reduction barrier. The silver cathode then catalyzes formation of the final products. Formation of gaseous CO is first observed at an applied voltage of 1.5 volts, just slightly above the minimum (i.e., equilibrium) voltage of 1.33 volts. The system continued producing CO for at least 7 hours at Faradaic efficiencies greater than 96%.     

Climate change,human impacts,and the resilience of coral reefs

The diversity, frequency, and scale of human impacts on coral reefs are increasing to the extent that reefs are threatened globally. Projected increases in carbon dioxide and temperature over the next 50 years exceed the conditions under which coral reefs have flourished over the past half-million years. However, reefs will change rather than disappear entirely, with some species already showing far greater tolerance to climate change and coral bleaching than others. International integration of management strategies that support reef resilience need to be vigorously implemented, and complemented by strong policy decisions to reduce the rate of global warming.     

A cDNA for a protein that interacts with the human immunodeficiency virus Tat transactivator

The human immunodeficiency virus (HIV) tat protein (Tat) is a positive regulator of virus gene expression and replication. Biotinylated Tat was used as a probe to screen a lambda gt11 fusion protein library, and a complementary DNA encoding a protein that interacts with Tat was cloned. Expression of this protein, designated TBP-1 (for Tat binding protein-1), was observed in a variety of cell lines, with expression being highest in human cells. TBP-1 was localized predominantly in the nucleus, which is consistent with the nuclear localization of Tat. In cotransfection experiments, expression of TBP-1 was able to specifically suppress Tat-mediated transactivation. The strategy described may be useful for direct identification and cloning of genes encoding proteins that associate with other proteins to modulate their activity in a positive or negative fashion.     

Novel regulators of bone formation: molecular clones and activities

Protein extracts derived from bone can initiate the process that begins with cartilage formation and ends in de novo bone formation. The critical components of this extract, termed bone morphogenetic protein (BMP), that direct cartilage and bone formation as well as the constitutive elements supplied by the animal during this process have long remained unclear. Amino acid sequence has been derived from a highly purified preparation of BMP from bovine bone. Now, human complementary DNA clones corresponding to three polypeptides present in this BMP preparation have been isolated, and expression of the recombinant human proteins have been obtained. Each of the three (BMP-1, BMP-2A, and BMP-3) appears to be independently capable of inducing the formation of cartilage in vivo. Two of the encoded proteins (BMP-2A and BMP-3) are new members of the TGF-beta supergene family, while the third, BMP-1, appears to be a novel regulatory molecule.