美国东部黑核桃不同家系幼树年生长节律研究

通过对引进的3个家系美国东部黑核桃4-5年生幼树的年生长节律及物候分析比较,结果表明:(1)黑核桃春季萌动、抽梢生长较晚,生长期仅为80 d左右,高、径生长集中在5-7月;(2)为使新梢充分木质化,预防2次生长带来的早、晚霜危害,树木停止高生长后,及时有效控水;(3)同一种源不同家系间,高、径生长存在差异,引进种子苗木时,应考虑引进经过区域试验种源的优良家系。     

Long-term development of fish populations in the Římov Reservoir

Abstract  The inshore fish community of the Římov Reservoir in the Czech Republic was evaluated over 21 years using shore seining at night. The development of the fish community was divided into two separate phases: a highly dynamic and unstable phase dominated by perch, Perca fluviatilis L., was replaced by an extremely stable cyprinid phase dominated by roach, Rutilus rutilus (L.), and bream, Abramis brama (L.). The abundance of both these species oscillated during the cyprinid phase, but with decreasing amplitude. The proportion of piscivorous fish species such as asp, Aspius aspius (L.), pike, Esox lucius L., and pikeperch, Sander lucioperca (L.), increased slightly with time but remained low. The biomass of large Cladocera was negatively correlated with fish biomass only during the perch phase. The Shannon–Weaver index of diversity increased during the fish community succession, mainly because of greater evenness amongst the species.     

Population structure of Carapa guianensis in two forest types in the southwestern Brazilian Amazon

Carapa guianensis Aublet. is a tropical tree with strong multiple-use characteristics, and is valued for both the high quality oil extracted from its seeds and as a timber resource. This study compares the population structure of this economically important rainforest tree in two contrasting forest types: occasionally inundated and terra firme forests. Main study objectives were (a) to assess the density, spatial distribution, and size class structure of C. guianensis in these two forest types and (b) to use patterns of abundance, distribution and demographic structure to help infer key demographic stages or ecological variables that merit special focus for management. Four 400 m × 400 m plots, two in each forest type, were established to determine distribution and density patterns of C. guianensis ≥10 cm diameter at breast height (dbh) at the landscape level, and 32 10 m × 10 m subplots were randomly nested within each of the larger plots to measure individuals <10 cm dbh. Larger individuals (≥10 cm dbh) were found at higher densities in occasionally inundated forest than in terra firme forest: 25.7 trees ha⁻¹ and 14.6 trees ha⁻¹, respectively. Mean density of C. guianensis individuals <10 cm dbh was also higher in occasionally inundated forests, but variation of regeneration density among the subplots was high. Spatial distribution methods revealed a tendency toward clumping in both forest types, and both had similar size class structures, suggesting that both environments are suitable for C. guianensis. This new finding illustrates the potential for C. guianensis management in terra firme forests. High densities and clumped distributions in both forest types are also indices favorable for sustainable species management. Finally, several ecological variables (tree density and reproductive potential) were sufficiently different between terra firme and occasionally inundated forests to recommend stratification by forest type when conducting further studies on key ecological and management variables of C. guianensis.     

肉牛早期断奶试验研究

[目的]旨在研究早期断奶技术对不同品种肉用犊牛生长发育的影响,[方法]本试验在草原牧区应用犊牛代乳料产品开展了不同品种犊牛的早期断奶工作,[结果]表明:安杂牛、新褐杂牛、荷斯坦牛三组间日增重差异不显著(P>0.05),安杂组增重成本8.25元/kg,新褐杂增重成本组9.27元/kg,荷斯坦组增重成本9.62元/kg,但...     

Pyloric outflow obstruction secondary to sclerosing encapsulating peritonitis in a dog

A 6-year-old, male neutered mixed breed dog was presented emergently with a three-week history of hyporexia, vomiting, diarrhoea and weight loss. Upon examination, the patient was dull, had generalised muscle atrophy, moderate abdominal pain and a mild amount of peritoneal effusion. A fluid-filled, distended, corrugated small bowel with marked gastroparesis and moderate peritoneal effusion was noted on abdominal ultrasonography. Endoscopy revealed hyperaemic and friable mucosa and a subjectively narrowed pylorus. Emergency exploratory celiotomy was performed due to worsening patient condition and revealed thick, diffuse, fibrous adhesions of the abdominal cavity. Based on these findings, sclerosing encapsulating peritonitis (SEP) was suspected. A large mass of omentum adjacent to the greater curvature of the stomach had caused a pyloric outflow obstruction. Adhesiolysis was attempted but was unsuccessful due to the friability of the small intestines. The dog was humanely euthanased under anaesthesia. A diagnosis of SEP was confirmed via necropsy. No underlying cause was identified. This is the first known case of a pyloric outflow obstruction secondary to SEP in a dog. Although rare, this condition should be considered as a differential for dogs with signs of a pyloric outflow obstruction with concurrent ascites and abdominal pain, hyporexia, vomiting and diarrhoea.     

Detection and isolation of Toxoplasma gondii from fresh semen of naturally infected dogs in Southern Brazil

The aim of this study was to isolate Toxoplasma gondii and determine the viability of the parasite in fresh semen samples of clinically healthy adult dogs naturally infected. Eleven seropositive dogs with T. gondii IgG antibodies from southern Brazil were selected to confirm the presence and viability of T. gondii in fresh semen samples using in vitro isolation in Vero cell culture, polymerase chain reaction (PCR) and sequencing analysis. The presence of viable T. gondii was confirmed by in vitro isolation and PCR in five semen samples. The ITS1 region of the isolated protozoa (TG S4) was amplified and sequenced. The nucleotide sequence obtained was 99% compatible with the T. gondii DNA sequences stored in the GenBank. It has been shown that T. gondii tachyzoites may be isolated in vitro from fresh semen samples of clinically healthy dogs seropositive for T. gondii.     

Pregnancy-associated Glycoprotein Profile during the First Trimester of Pregnancy in Egyptian Buffalo Cows

Pregnancy-associated glycoprotein (PAG) concentrations were measured in buffalo cows starting from day 28 after breeding. Oestrus was synchronized in 10 buffaloes using two injections of 25 mg prostraglandin (PG)F_{2 α} (Lutalyse^®) at a 11-day interval. Blood sampling was conducted nearly twice weekly. Results indicated that plasma PAG concentrations in non-pregnant buffaloes were low (<0.20 ng/ml) during the whole experimental period (day 28 to 103), while in pregnant animals plasma PAG levels increased from day 28 (4.48 ± 0.92 ng/ml) until day 41 (27.27 ± 6.74 ng/ml), remaining high (20.71 ± 9.20 ng/ml) until day 103. Progesterone levels were significantly (p < 0.0001) higher in pregnant (3.51–4.80 ng/ml) than in non-pregnant buffaloes (0.28–1.52 ng/ml). A significant difference (p < 0.0001) in plasma PAG concentrations between pregnant and non-pregnant animals starting at day 28 after breeding suggests that PAG-radioimmunoassay could be suitable for pregnancy diagnosis in buffaloes during this period. In conclusion, PAG test offers the advantages that it requires a single plasma sample for early pregnancy diagnosis as well as the accuracy of the test for the detection of pregnancy as early as day 28.     

Demographic threats to the sustainability of Brazil nut exploitation

A comparative analysis of 23 populations of the Brazil nut tree (Bertholletia excelsa) across the Brazilian, Peruvian, and Bolivian Amazon shows that the history and intensity of Brazil nut exploitation are major determinants of population size structure. Populations subjected to persistent levels of harvest lack juvenile trees less than 60 centimeters in diameter at breast height; only populations with a history of either light or recent exploitation contain large numbers of juvenile trees. A harvesting model confirms that intensive exploitation levels over the past century are such that juvenile recruitment is insufficient to maintain populations over the long term. Without management, intensively harvested populations will succumb to a process of senescence and demographic collapse, threatening this cornerstone of the Amazonian extractive economy.     

Market Integration and Livelihood Systems: A Comparative Case of Three Asháninka Villages in the Peruvian Amazon

ABSTRACT

The strong trend in indigenous societies toward market integration often results in loss of traditional cultural elements and changes in livelihood systems. This comparative study of three Asháninka villages located at different distances to market had two objectives: (a) to describe and analyze the current livelihood system of the three villages in terms of their goals, activities, and resources; and (b) to analyze the livelihood system in relation to market integration at both village and household levels. Two variables were used to measure market integration: (a) quantification of the value of Western goods at the household level, and (b) area under cultivation per household designated to both household subsistence and external market. We found that although walking time to market was inversely proportional to market integration and directly proportional to traditional practices at the village level, two different trends of market integration were found at the household level: (a) basic market integration, clearly apparent in households at all three villages; and (b) high market integration, found solely in the two villages closest in time to market. The existence of market–integrated, less traditional households side by side with minimally integrated, traditional households appeared to be linked to differences in household goals and strategies and perhaps availability of male labor. Market integration increased workloads on productive activities oriented to the market for both genders, but only men marketed these products for sale.     

Bluetongue virus infection in sheep: haematological changes and detection by polymerase chain reaction

SUMMARY Eight sheep vaccinated with 10⁶ pfu of attenuated Australian bluetongue virus serotype 23 (BTV-free sheep were challenged with virulent BTV 23. There was little subsequent variation in the mean clinical score, or in the mean lymphocyte and platelet concentrations in the peripheral blood of the eight vaccinated sheep. There was a marked thrombocytopenia and lymphopenia in the naive sheep as the mean lymphocyte and platelet concentrations fell to a minimum at days 8 and 11 after inoculation, respectively. Similar changes were observed in three other naive sheep inoculated with field isolates of BTV 1, 9 or 23. BTV was detected by nested polymerase chain reaction in whole blood of these sheep between days 6 and 28, in mononuclear leukocytes between days 3 and 14, and in platelets between days 6 and 21.