低温对库尔勒香梨不同部位抗氧化酶活性的影响

为了解香梨不同部位的抗寒能力与抗氧酶活性是否具有相关性,以库尔勒香梨为材料,测定不同部位越冬过程中抗氧化酶活性的变化。结果表明,库尔勒香梨不同部位在越冬过程中体内SOD,POD,CAT活性有明显的变化。SOD活性变化总体规律都随温度的上升而降低,不同部位之间的SOD活性没有明显差异;各部位的SOD活性不同月份之间有明显的差异,在最为寒冷的1月份达到最高值,其中休眠芽的活性为最高。POD活性随温度的逐渐升高而上升,3月份达到最高,其中主干阳面和阴面的POD活性高于其他部位。阳面和阴面CAT活性随温度的提高而回升,2月份最低,休眠芽和枝条CAT活性均逐渐下降。     

Level and period of realimentation to assess improvement in body condition and carcass quality in cull ewes

Improvement in body condition was assessed in 40 cull ewes (>6 years), equally distributed in two groups and realimented with ad libitum roughage (gram straw) and two levels of concentrate feeding, i.e., 2.5 % (T₁) of live weight (LW) and ad libitum (T₂). Five representative animals from an initial 45 were slaughtered at the initiation of the study (0 day) and five animals from each treatment at 44, 67, and 90 days of experiment for carcass attributes. Improvement in body condition score (BCS), nutrient utilization, feed efficiency, and carcass traits were assessed at 44, 67, and 90 days. Metabolism trial of 6-day collection of feed, feces, and urine samples was conducted on five representative ewes from each group after 60 days of feeding. The level of concentrate feeding on LW gain and BCS was significant, and the duration of realimentation showed a linear improvement (P?<?0.001). The digestibility and intake of dry matter, organic matter, and crude protein was higher (P?<?0.05) in T₂. The N intake, absorption, and balance showed a similar trend. Increase (P?<?0.05) in total N, trichloroacetic acid precipitable N, and ammonia N was observed with extension of realimentation period. Blood metabolic profile also showed improvement (P?<?0.05) from an undernourished state to normal after alimentation. Animals in T₂ accumulated higher LW with minimal expenditure of metabolizable energy (73.4 vs 79.1 MJ) and higher efficiency of feed conversion during 68 to 90 days of realimentation. Linear improvement (P?<?0.01) in carcass traits (preslaughter weight, empty live weight, hot carcass weight, dressing percentage, and amount of subcutaneous and intramuscular fat) and composition of longissimus dorsi muscle was observed. Ad lib concentrate supplementation for a period of 90 days may thus be considered appropriate for achieving desired efficiency of gain and improvement in body condition of cull ewes for quality mutton production.     

Effects of variation in seed sources and pre-sowing treatments on seed germination of Tamarindus indica: a multi-purpose tree species in Bangladesh

We investigated the morphology and germination of Tamarindus indica seeds in order to discover the effects of variation in seed sources and pre-sowing treatments on germination. The experiment was carried out in the nursery of the Forestry and Wood Technology Discipline, Khulna University, Bangladesh. Seeds were collected from different but healthy trees from various locations in Bangladesh and subjected to four pre-sowing treatments, i.e., control, immersion in cold water (4°C for 24 h), immersion in hot water (80°C for 10 min) and scarification with sand paper. Seed germination was carried out in poly-bags with a mixture of topsoil and cow dung in the ratio of 3:1. The average length of the fresh seeds was 1.35±0.26 cm, their width 1.07 ±0.20 cm and thickness 0.69±0.11 cm. The results revealed that pre-sowing treatments affected the germination process of seeds, which significantly increased the germination percentage with the cold water (81.67%) and scarification with sand paper (82.33%) treatments, compared with those in the control (58.33%) and hot water treatment (59.00%). In all treatments germination started on average within 5 to 7 days after the start of the treatments and were completed from 13 to 19 days later. The greatest success in germination (82.33%) was found in scarification with sand paper, followed with 81.67% in the cold water treatment. The results of analyses of variance (ANOVA) showed significant differences (p<0.05) in germination closing dates, seed germination periods and germination percentages among the treatments but no significant difference among seed sources. However, the effect of the interaction between seed source variation and pre-treatment differed significantly in seed germination closing dates and germination percentages. We recommend cold water treatment (submersion of seed at a temperature of 4°C for a 24-h period) for seed germination of T. indica in rural Bangladesh.     

Novel and simple approach using synthesized nickel nanoparticles to control blood-sucking parasites

The present study was on assessment of the anti-parasitic activities of nickel nanoparticles (Ni NPs) against the larvae of cattle ticks Rhipicephalus (Boophilus) microplus and Hyalomma anatolicum (a.) anatolicum (Acari: Ixodidae), fourth instar larvae of Anopheles subpictus, Culex quinquefasciatus and Culex gelidus (Diptera: Culicidae). The metallic Ni NPs were synthesized by polyol process from Ni-hydrazine as precursor and Tween 80 as both the medium and the stabilizing reagent. The synthesized Ni NPs were characterized by Fourier transform infrared (FTIR) spectroscopy analysis which indicated the presence of Ni NPs. Synthesized Ni NPs showed the X-ray diffraction (XRD) peaks at 42.76°, 53.40°, and 76.44°, identified as 1 1 1, 2 2 0, and 2 0 0 reflections, respectively. Scanning electron microscopy (SEM) analysis of the synthesized Ni NPs clearly showed that the Ni NPs were spherical in shape with an average size of 150 nm. The Ni NPs showed maximum activity against the larvae of R. (B.) microplus, H. a. anatolicum, A. subpictus, C. quinquefasciatus and C. gelidus with LC₅₀ values of 10.17, 10.81, 4.93, 5.56 and 4.94 mg/L; r² values of 0.990, 0.993, 0.992, 0.950 and 0.988 and the efficacy of Ni-hydrazine complexes showed the LC₅₀ values of 20.35, 22.72, 8.29, 9.69 and 7.83 mg/L; r² values of 0.988, 0.986, 0.989, 0.944 and 0.978, respectively. The findings revealed that synthesized Ni NPs possess excellent larvicidal parasitic activity. To the best of our knowledge, this is the first report on larvicidal activity of blood feeding parasites using synthesized Ni NPs.     

Field evaluation of some bait additives against Indian crested porcupine (Hystrix indica) (Rodentia: Hystricidae)

This research study evaluated the effect of different additives on the bait consumption by Indian crested porcupine, a serious forest and agricultural pest, under field conditions. Different additives (saccharin, common salt, bone meal, fish meal, peanut butter, egg yolk, egg shell powder, yeast powder, mineral oil and coconut oil) at 2 and 5% each were tested for their relative preference, using groundnut–maize (1:1) as basic bait. All the additives were tested under a no‐choice test pattern. For control tests, no additive was mixed with the basic bait. Saccharin at 5% concentration significantly enhanced the consumption of bait over the basic bait, while 2% saccharin supplemented bait resulted in a non‐significant bait consumption. All other additives did not enhance the consumption of the bait material; rather, these worked as repellents. However, the repellency was lowest with the common salt, followed by egg yolk, egg shell powder, bone meal, peanut butter, mineral oil, fish meal and yeast powder, while coconut remained the most repellent compound. The present study suggested that groundnut–maize (1:1) supplemented with 5% saccharin was the preferred bait combination, and can be used with different rodenticides for the management of Indian crested porcupine.     

小麦茎生长点转化研究初报

为了躲避转基因技术对组织培养的过分依赖,本研究以小麦为材料尝试了一种对生长点直接进行转化的方法,并初步证明了这一方法的可行性。具体做法是：将种子萌芽,然后剥去胚芽鞘暴露出生长点,再用玻璃纤维制作的小刷子将生长点刺伤,最后用带有外源基因的农杆菌进行侵染处理。用携带hph-GUS基因的LBA4404农杆菌,侵染处理1～11日龄幼苗(生长点),共培养7d后检测新生芽中GUS的瞬时表达情况;侵染1～2日龄幼苗(生长点),取长成植株的2～3朵小花进行GUS稳定表达检测。又用携带BADH和npt Ⅱ基因的AGL1农杆菌菌株侵染1日龄的幼苗(生长点),在含：100mg／L卡那霉素的蛭石中进行选择。结果表明,GUS的瞬时表达率随被处理幼苗的日龄增加而降低,以2日龄幼苗为最高(10．7％)。用花序检测GUS的稳定表达,被侵染受体为1日龄幼苗时的表达率高于2日龄的幼苗。蔗糖的存在并不提高GUS基因在花序中表达的频率。不过花序表现为GUS阳性的植株后代,经PCR检测后并没有证实GUS基因存在。用AGL1菌株进行转化,获得了13．6％的抗卡那霉素的绿色植株,但只有3株呈现PCR阳性,且只有1株结了实。     

Preliminary Study of Variable Rate Application – Organic Liquid Fertilizer by Using SPAD Chlorophyll Meter on System of Rice Intensification (SRI) Cultivation

Knowing actual nutrient requirements for rice plants is crucial in supplying the correct amount of fertilizer, especially nitrogen since nitrogen is one of the most key limiting nutrients in rice cultivation. A preliminary study of variable rate application (VRA) on System of Rice Intensification (SRI) planting by using organic foliar fertilizer was carried out to determine the effectiveness of precision fertilization. Foliar fertilizer was formulated based on actual nitrogen needed by rice plants using Soil-Plant Analyses Development (SPAD) chlorophyll meter measurements. The experiment was laid out in a randomized complete block design (RCBD) with four treatments (50% fixed rate, 100% fixed rate, 150% fixed rate and VRA) and four replications for each treatment. Plant growth performances (plant heights, number of tillers, number of panicle and flower) and yield performances (grain yield, number of grain, 1000-grain weight and number of spikelets) were recorded during the study. The results showed that foliar application of VRA resulted in significantly higher yield performances; grain yield (13.65 g), number of grain (807.50), 1000-grain weight (16.79 g), and number of spikelets (7.50) compared to uniform fertilizer applications. VRA treatments had the highest SPAD readings at every planting stage during the experiment; however, a high nitrogen supply was needed during the mid-tillering stages (35 DAT) compared to other fertilizer rates. Besides, VRA application shows the most savings in term of total nitrogen supply (415 µg) compared to uniform rates application; 50% fixed rate (400 µg), 100% fixed rate (810 µg), and 150% fixed rate (1210 µg).     

Physicochemical Characteristics of Biochars Derived From Corn,Hardwood, Miscanthus,and Horse Manure Biomasses

Biochar, a carbon-rich by-product of biomass pyrolysis, is widely recognized as a potential ingredient for soil amendment, fertility, and carbon sequestration owing to its favorable physicochemical properties. The objective of this study was to investigate the chemical and physical properties of biochars produced through pyrolysis at 450°C from agricultural residues available in Northwest Missouri, namely hardwood (HW), corn stover (CR), miscanthus (MS), and horse manure (HM). These properties were assessed through the analysis of pyrolysis yield, pH, volatile matter, fixed carbon, ash and carbon (C), hydrogen (H), sulfur (S), nitrogen (N) contents, trace metal concentrations, surface morphology, surface functional groups, bulk density, and water holding capacity. The biochars derived from HW, MS and CR materials showed high volatile-matter (33–42%), high fixed carbon contents (42–47%), very low ash contents (6–15%), and low bulk density (0.14–0.28 g cm^?3) as compared to that of HM. A wide range of trace elements was observed in biochar samples with significant differences in concentrations. In addition, CR, HW and MS biochars displayed a disordered graphitic-like structure with well-developed pores and surface areas of 23, 70 and 90 m²/g respectively, and high water-holding capacity up to 750%, indicating their potential application as a soil amendment.     

Nestedness and modularity in fragmented Shasha Forest Reserve,southwestern Nigeria

Species co-occurrence and nestedness pattern in Shasha forest reserve were investigated with a view to determining the effect of interactions to species contribution. Three distinct sites (Gmelina arborea plantation, undisturbed forest, and Pinus carribaea (plantation) were selected in the forest reserve. Four 25 m × 25 m plots were established in each of the site within the forest reserve. Species enumeration, identification,, and distribution into families of the standing vegetation were carried out. Woody species represented the most diverse life form. Plant species diversity was highest in UF and lowest in Gmelina plantation. Differences in woody species composition among the three forest types were determined by non-metric multidimensional scaling (NMDS) analysis, analysis of similarity (ANOSIM), and similarity of percentages. Using z-score and NODF (a nestedness metric based on overlap and decreasing fill). Three modules were detected and modularity score Q = 0.2. The composition analyses (NMDS and ANOSIM) revealed a significant difference in the woody species composition among the forest types. Our research may contribute to understand species assemblage in one of the diverse forests of Africa. Current work highlighted species occurrence in Shasha forest and highlighted the importance of non-random pattern which could serve as framework for conservation perspective.     

Comparison of protection and viral shedding following vaccination with Newcastle disease virus strains of different genotypes used in vaccine formulation

Tropical Animal Health and Production - Newcastle disease (ND) is a devastating disease and cause high t mortality and morbidity in poultry and nonpoultry avian species worldwide. An intensive...