Malt hydrolysates for gluten-free applications: Autolytic and proline endopeptidase assisted removal of prolamins from wheat,barley and rye

Cereal based products intended for gluten sensitive individuals, particularly to celiac disease patients, tend to have poor organoleptic qualities and they contain low levels of healthy whole grain compounds. Adding whole grain ingredients, such as malt hydrolysates, could compensate these defects provided that the ingredients are adequately free from toxic prolamin epitopes. Here we demonstrate that the level of toxic prolamin epitopes in the malt autolysates (wheat, barley, rye) were substantially lower than in the native malts but too high to allow “very low in gluten” labelling. To further eliminate the residual levels of toxic prolamin epitopes, a proline-specific endoprotease from Aspergillus niger was added to the malt autolysates. In the resulting malt hydrolysates (of wheat and rye but not barley), the prolamins were indeed greatly reduced and were below the very low gluten limit of 100 mg/kg. Malt hydrolysates with adequately low gluten levels may potentially be used as novel ingredients within gluten-free foods.     

The effects of soil and air temperature on CO2 exchange and net biomass accumulation in Norway spruce, Scots pine and silver birch seedlings

Soil temperature is proposed to affect the photosynthetic rate and carbon allocation in boreal trees through sink limitation. The aim of this study was to investigate the effect of temperature on CO(2) exchange, biomass partitioning and ectomycorrhizal (ECM) fungi of boreal tree species. We measured carbon allocation, above- and below-ground CO(2) exchange and the species composition of associated ECM fungi in the rhizosphere of Scots pine (Pinus sylvestris L.), Norway spruce (Picea abies K.) and silver birch (Betula pendula Roth) seedlings grown in soil maintained at 7-12, 12-15 and 16-22 °C. We found increased root biomass and photosynthetic rate at higher soil temperatures, but simultaneously with photosynthesis rate, higher temperature generally increased soil respiration as well as shoot, and root and rhizosphere respiration. The net CO(2) exchange and seedling biomass did not increase significantly with increasing temperature due to a concomitant increase in carbon assimilation and respiration rates. The 2-month-long growth period in different soil temperatures did not alter the ECM fungi species composition and the below-ground carbon sink strength did not seem to be directly related to ECM biomass and species composition in any of the tree species. Ectomycorrhizal species composition and number of mycorrhiza did not explain the CO(2) exchange results at different temperatures.     

Loss function analysis of private forest owners’ timber price change expectations

Loss function analysis was introduced to timber market price analysis. Methods found in Bayesian statistical decision analysis were applied to expectations of market price changes. These were obtained from a forest owner survey conducted in Finland in 2009. Forecast errors of heterogeneous price expectations of the individual private forest owners were derived with observed price changes in six different timber price regions. The forecast errors were complemented with random number simulations to control for unobserved response heterogeneity. The properties and estimation of generalized loss functions – LIN-EX and flexible loss functions – allowing for asymmetry in individual forecast errors were introduced into the analysis. Estimation results for different price regions implied that forest owners’ perceived costs related to positive forecast errors of negative price changes that took place in 2009 were larger than those related to negative errors. This under-prediction vs. over-prediction asymmetry was large in some price regions. The forest owners exhibited an aversion to losses reflected in price expectations errors in the year 2009. The observed loss aversion means that forest owners were cautious in their selling decisions during periods of declining market prices. This has a negative impact on the recovery of slack timber markets.     

Influence of thiol metabolism of lactobacilli on egg white proteins in wheat sourdoughs

In wheat sourdoughs, the degradation of gluten proteins is favored by acidification and reducing conditions. This study aimed to determine the proteolytic degradation of egg white proteins in wheat sourdoughs acidified with lactobacilli differing in their thiol metabolism. Ovotransferrin was the only major egg white protein that degraded during sourdough fermentations. An extensive degradation of ovotransferrin required a heterofermentative lactobacilli starter, Lactobacillus sanfranciscensis, with glutathione reductase activity. Ovotransferrin was more resistant to breakdown when sourdoughs were acidified with homofermentative lactobacilli or a mutant strain of L. sanfranciscensis lacking the glutathione reductase. Its susceptibility to proteolysis in L. sanfranciscensis sourdoughs is thus attributable to thiol accumulation by L. sanfranciscensis, which apparently altered the structure of ovotransferrin through a reduction of disulfide bonds. Proteolytic degradation of ovotransferrin was attributable to wheat aspartic proteinases. In addition to the susceptibility to proteolysis, other functional properties of egg proteins may be influenced by thiol-exchange reactions.     

Evaluation of reforestation potential of 83 tree species planted on Imperata cylindrica dominated grassland – A case study from South Kalimantan,Indonesia

Survival and growth of 83 tree species were tested in three separate species elimination trials on Imperata cylindrica dominated grassland in South Kalimantan, Indonesia. The trial layout was randomized complete blocks design with 6–8 replications of 5-tree line plots. At the age of two years exotics, like several Acacia species (A. mangium, A. crassicarpa, A. auriculiformis, A. cincinnata, A. leptocarpa), as well as Gmelina arborea, Paraserianthes falcataria and Cassia siamea had survival rates of 90–100%, crown width of 3–6 m and mean height of 5–8 m. Among the most promising local species were Anthocephalus chinensis, Peronema canescens and Parkia roxburghii but their growth was inferior compared to exotics. Eucalypts and pines were not among the highest ranking trees and cannot be recommended for initial grassland reforestation. At the age of 7–8 years the same species were still dominating, but increasing mortality of several species was observed. Especially Acacia crassicarpa and A. cincinnata of the exotics were affected, and indigenous Anthocephalus chinensis almost perished. Since fast early growth is necessary in first rotation reforestation of grasslands, fast growing exotic species provide a reliable basis for further activities. Final performance of reforestation species has to be confirmed in stand-level experiments and pilot plantations. Intensive work on integrating local tree species in reforestation of grasslands in the long run is recommended.     

Degradation of Secalins During Rye Sourdough Fermentation

Rye sourdough (RSD) gives rye bread mildly acidic taste and desired flavor. Flavor precursors (amino acids and small peptides) are generated in the proteolytic breakdown of rye proteins. Our aim was to study the protein degradation during RSD fermentations. Two sourdoughs were prepared of flours derived from two rye cultivars (Amilo and Akusti). RSD samples were collected during fermentations. Three protein fractions were obtained by sequential protein extraction and these were analyzed by SDS‐PAGE. Free amino nitrogen (FAN) was measured with a ninhydrin method. In addition, two rye incubations without starter microorganisms (with antibiotics) were made at pH 3.6 and 6.1, and proteinase profiles of the rye cultivars were analyzed at pH 4.3. SDS‐PAGE analysis showed that during RSD fermentations, rye proteins, especially the alcohol‐soluble secalins, were degraded. Secalins also evidently degraded during the incubation without starter microorganisms at pH 3.6. Aspartic proteinases were in the major proteinase group in both rye cultivars. This study confirms that endogenous proteinases of rye, mainly aspartic proteinases, hydrolyze rye proteins, especially secalins, during RSD fermentation. Protein degradation in rye sourdoughs may thus be enhanced by selecting rye flours with high proteolytic activity toward secalins.     

Effects of ISPOLIN Fertilizer on Uptake of Macro- and Trace Elements by Wheat Grown in Two Different Soils

Variations in concentrations of 24 elements in soils with loam and sandy loam texture and in Triticum aestivum wheat seedlings grown in the soils under greenhouse conditions were studied. Initial soils differed significantly in concentrations of 20 elements. Elemental composition of wheat seedlings depended on the soil where the plants were grown. An application of ISPOLIN (fertilizer enriched with potassium) resulted in variations in soil pH, plant biomass, and concentrations of several elements both in soils and in plants. An excess of bioavailable potassium (K) in soil led to significant increase of K and deficiency of calcium (Ca) and magnesium (Mg) in all parts of T. aestivum. Concentrations of many other elements in the plants also changed. The effects of soil fertilization on plant biomass, leaf chlorophyll, and plant element concentrations were soil-dependent. Depending on the ratio of K/Mg in the soils, there was either an increase of plant yield and chlorophyll content or no effect at all.     

Public cost-sharing in private forest investments in Finland 1983–2000

A model of public cost-sharing in private forest investment is proposed to describe the substitution between the private financing of investments and public investment assistance. The substitution depends on the curvature conditions of the forest investment function on forest stock. When the second-order investment effects are close to zero or when they do not exist, the funding substitution will not take place. A simultaneous econometric model for private and public funding employing forest incomes, forest income taxes, interest rates, investment scale and market wood price expectations as exogenous variables is estimated. The model estimation based on Finnish regional data in 1983–2000 rejects the substitution alternative. A 10% increase in private investment funding increases the demand for public funding at the same rate, but a 10% increase in public funds will increase the private funds supply by 2.5%. Significant income effects are found only in the case of private funding. In northern Finland, the scale effects are large for public financial assistance. The effects of the income tax reform on the private supply of funds are positive, especially for the new wood-sales profit taxation, whereas the interest rate and price expectation effects are negative.