Preparation and characterization of urea-formaldehyde resin-sodium montmorillonite intercalation-modified poplar

To improve its overall performance, fast-growing poplar was modified using the vacuum-pressure-vacuum impregnation method with a urea-formaldehyde resin-sodium montmorillonite intercalation as the modification solution. The results showed that considerable amounts of urea-formaldehyde resin and montmorillonites entered the poplar tracheid, and some entered the microporous wood. These substances formed bonds with the active groups in timber, causing reduced crystallinity in the amorphous region of the poplar, a decreased level of free hydroxyl, and an enhanced association with hydroxyl and ether bonds. The density, dimensional stability, and mechanical properties of poplar were markedly improved. The best results were obtained with 14% sodium montmorillonite and 20% ureaformaldehyde resin: the bending resistance, compressive resistance, and elastic modulus increased by 19.37%, 30.24%, and 50.06%, respectively. With elevated levels of sodium montmorillonite, the impact toughness and wear rate decreased.     

Effects of sealed press on improving the properties of particleboard

To improve the properties of particleboard, boards were produced using a sealed press. With the sealed press, boards were processed under high-temperature and high-pressure steam. This increased the saturation temperature, causing a dramatic rise in temperature inside the board, faster curing of the binder, and a shorter pressing time. The boards were bonded with urea formaldehyde resin, melamine urea formaldehyde resin, or poly(methylene diphenyl diisocyanate) (PMDI). The sealed press improved the internal bond strength and thickness swelling of boards regardless of the binder used during the reduced pressing time. The increased bonding strength improved the board properties, allowing PMDI with a lower resin content to be used for bonding the boards.     

Growth,P uptake and rhizosphere properties of wheat and canola genotypes in an alkaline soil with low P availability

The aim of the present study was to assess the role of soil type on growth, P uptake and rhizosphere properties of wheat and canola genotypes in an alkaline soil with low P availability. Two wheat (Goldmark and Janz) and two canola genotypes (Drum and Outback) were grown in a calcareous soil (pH 8.5) at two P levels [no P addition (0P) or addition of 200 mg kg⁻¹ P as Ca₃(PO₄)₂ (200P)] and harvested at flowering or maturity. Shoot and root dry weight, root length and shoot P content were greater in the two canola genotypes than in wheat. There were no consistent differences in available P, microbial P and phosphatase activity in the rhizosphere of the different genotypes. Shoot P content was significantly positively correlated with root length, pH and phosphatase activity in the rhizosphere. The microbial community composition, assessed by fatty acid methylester analysis, of the canola genotypes differed strongly from that of the wheat genotypes. The weight percentage bacterial fatty acids, the bacteria/fungi (b/f) ratio and the diversity of fatty acids were greater in the rhizosphere of the canolas than in the rhizosphere of the wheat genotypes. In contrast to the earlier studies in an acidic soil, only small differences in growth and P uptake between the genotypes of one crop were detected in the alkaline soil used here. The results confirmed the importance of root length for P uptake in soils with low P availability and suggest that the rhizosphere microbial community composition may play a role in the better growth of the canola compared to the wheat genotypes.     

Source identification and apportionment of soil cadmium in cropland of Eastern China: a combined approach of models and geographic information system

Purpose

Cadmium (Cd) is regarded as one of the most toxic heavy metals in the environment and can undermine the ecosystem function and human health at trace level due to its high toxicity. In order to reduce the anthropogenic Cd input into agricultural soil, it is of utmost importance to pinpoint the sources of Cd in soils and apportion their respective contributions.

Materials and methods

One hundred twenty-seven topsoil samples and 21 subsoil samples were collected from croplands of Meishan Basin, Changxing County, Zhejiang Province, Eastern China, and analyzed for concentrations of Cd and other heavy metals. Finite mixture distribution model (FMDM) was employed to fit the data to obtain the local soil Cd threshold value, a critical indicator to assess soil heavy metal contamination. Then, principal component analysis (PCA) and geographic information system (GIS) were used to identify the potential sources of Cd. Finally, positive matrix factorization (PMF) was applied to apportion the source contributions.

Results and discussion

Among the 127 topsoil samples, 71 were subject to Cd contamination with a mean concentration of 0.66 mg kg^?1 while the others were considered as background with a lower mean concentration of 0.145 mg kg^?1, close to the local background concentration of 0.142 mg kg^?1. Further, three components were extracted by PCA and interpreted as natural background, lead-acid battery manufacturing plants, and construction material associated activities, respectively. Additionally, most of the topsoil samples around the lead-acid battery manufacturing plants, construction material plants, and limestone/marble quarries were classified as Cd contaminated. However, PMF failed to get a successful portioning.

Conclusions

Lead-acid battery manufacturing plants and construction material associated activities were the main anthropogenic sources of soil Cd contamination. With the help of FMDM, it is possible to distinguish the contaminated soil and estimate the contribution of anthropogenic sources to soil Cd. The apportionment by PMF was not successful in this paper due to the high skewness or outliers of Cd concentration in sampling sites and violation of the assumption that all samples have the same sources.

     

Functional and structural responses of bacterial and fungal communities from paddy fields following long-term rice cultivation

Purpose

Rice paddy soils undergo pedogenesis driven by periodic flooding and drainage cycles that lead to accumulation of organic matter and the stratification of nutrients and oxygen in the soil profile. Here, we examined the effects of continuous rice cultivation on microbial community structures, enzyme activities, and chemical properties for paddy soils along a chronosequence representing 0–700 years of rice cropping in China.

Materials and methods

Changes in the abundance and composition of bacterial and fungal communities were characterized at three depths (0–5, 5–10, and 10–20 cm) in relation to organic carbon, total nitrogen, dissolved organic carbon, microbial biomass carbon/nitrogen, and activities of acid phosphatase, invertase, and urease.

Results and discussion

Both soil organic carbon and total nitrogen increased over time at all three depths, while pH generally decreased. Microbial abundance (bacteria and fungi) and invertase and urease activity significantly increased with the duration of rice cultivation, especially in the surface layer. Fungal abundance and acid phosphatase activity declined with depth, whereas bacterial abundance was highest at the 5–10-cm soil depth. Profiles of the microbial community based on PCR-DGGE of 16S rRNA indicated that the composition of fungal communities was strongly influenced by soil depth, whereas soil bacterial community structures were similar throughout the profile.

Conclusions

Soil bioactivity (microbial abundance and soil enzymes) gradually increased with organic carbon and total nitrogen accumulation under prolonged rice cultivation. Microbial activity decreased with depth, and soil microbial communities were stratified with soil depth. The fungal community was more sensitive than the bacterial community to cultivation age and soil depth. However, the mechanism of fungal community succession with rice cultivation needs further research.

     

Assessment of changes in soil organic matter after invasion by exotic plant species

Invasive exotic plants can modify soil organic matter (SOM) dynamics and other soil properties. We evaluated changes in particulate organic matter (POM) and carbon (C) mineralisation in adjacent plots invaded by Solidago gigantea, Prunus serotina, Heracleum mantegazzianum and Fallopia japonica, and non-invaded control plots on different soils in Belgium. Litter decomposition of S. gigantea and P. serotina was compared to that of the native species Epilobium hirsutum, Betula pendula and Fagus sylvatica. Disregarding the differences in site characteristics (soil texture, parental material and plant species), we argued that the invasion by S. gigantea and P. serotina enhance SOM dynamics by increasing C mineralisation in 2 out of 3 sites invaded by S. gigantea and in 1 out of 3 sites invaded by P. serotina; C in coarse POM (cPOM, 4,000–250 μm) and fine POM (fPOM, 250–50 μm) in 1 site invaded by S. gigantea and C content in total POM (tPOM, 4,000–50 μm) and the organo-mineral fraction (OMF, 0–50 μm) in 1 site invaded by P. serotina. H. mantegazzianum and F. japonica slowed down SOM dynamics by reducing C mineralisation in three out of four sites; C and nitrogen (N) of fPOM in the invaded compared with the non-invaded plots at one site invaded by H. mantegazzianum. However, N content of cPOM (4,000–250 μm) was higher in the invaded sites by F. japonica compared with the non-invaded plots. Our results indicated that the effects of invasion by exotic plant species were not species-specific but site-specific.     

Using perlite as a substrate carrier for measuring microbial available phosphorus by respiration kinetics in soils

The release of CO₂ by soil microorganisms after the addition of nitrogen and glucose in excess and calibration additions of phosphorus has successfully been used to assess microbial available P, assuming the native soil P pool is then limiting respiration. However, in P-fixing soils and soils with high P content, carbon can be exhausted before the available soil P pool. It is not possible to simply increase the amount of glucose as then the glucose concentration would be lethal for microorganisms. A modified method was tested where soil is mixed with perlite. It was hypothesised that perlite, having a high water holding capacity, would dilute the concentration of glucose, while maintaining the bioavailability of added nutrients, thus avoiding carbon limitation. Factorial combinations of amount of soil and perlite (both adjusted to −25 kPa water potential) were tested to examine if perlite as such had any effect on the respiration. Five tropical soil samples with a sharp gradient in P availability and one N-limited compost material were used. The method successfully reduced the risk of carbon limitation. Microbial indices, such as basal respiration, substrate-induced respiration and maximum P-limited respiration, were directly proportional to the amount of soil in the experiments but unrelated to the amount of perlite, showing that perlite did not affect microbial measurements.     

Analysis of indigenous production methods and farm-based biodiversity of amochi (<Emphasis Type="Italic">Arisaema schimperianum</Emphasis> Schott) in two sub-zones of Southern Ethiopia

For the first time we report that diverse types of Arisaema schimperianum Schott locally called amochi, are grown and managed as an off-season food crop in Southern Ethiopia. Participatory Rural Appraisal (PRA) and formal survey studies on 204 households were conducted over two consecutive years (2003–2004) in Chencha and Dita sub zones of Southern Ethiopia, to identify indigenous production methods and farm based biodiversity of amochi. Amochi is propagated from seed tubers and tuber parts left in the soil from the preceding harvest and grow during the dry season, February to June. Average share of proportion in the annual household consumption from amochi tubers was 9% and 4% for Chencha and Dita respectively. Uncooked amochi is irritating in contact to skin and mouth. Fifteen amochi types were identified in Dita and 12 in Chencha. They differ in the level of irritation, maturity and yield. Rich farmers with large family size as a working force, large farm size to support larger number of livestock that is source of farmyard manure, have farms with larger diversity of amochi than their poor counterparts, suggesting that its diversity is influenced by house hold characteristics and favoured under relatively fertile soil conditions. In both sub zones women had better experience in identifying amochi types than men. Further study is needed to identify chemical contents; and genetic variation using morphologic and molecular traits, in order to develop a strategy to assess and utilise the existing genetic diversity.     

Powdery Mildew Resistance in a Collection of Chinese Barley Varieties

One hundred and forty-seven Chinese barley varieties maintained at the Gene Bank of the National Barley Improvement Centre, Zhejiang, and 84 progenies from these varieties were tested at the seedling stage for their reaction to 32 selected pathotypes of Blumeria graminis f. sp. hordei. Eighteen resistance spectra were identified comprising single or combined resistances from eight known (Ml(Bw), Ml(Ch), Mla7, Mla8, Mla9, Mla13, MlaRu4 and Mlg) and six unknown resistance genes. The most frequent gene was Ml(Bw), which was found in 69 varieties and previously detected in only a few European winter barley varieties. The genes Mla8 and Ml(Ch) were also often present, but other resistance genes were rare. After inoculation, considerably fewer pathogen colonies were observed in ‘Aiganqi’ and one line of ‘Tong5’. Twenty varieties were composed of lines with different resistance genes. China is likely to be a region of origin of the genes Ml(Bw), Mla7, as well as three unknown genes found in original landraces and perhaps another three unknown genes detected in cultivars bred in China. The resistances of varieties from the Zhejiang province and those originating from 11 other Chinese provinces were quite different. Unfortunately, none of the varieties are promising sources of resistance to powdery mildew and China does not seem to be a region suitable for identifying such sources.     

Comparison of genetic variation among accessions of <Emphasis Type="Italic">Aegilops tauschii</Emphasis> using AFLP and SSR markers

Genetic diversity of 54 accessions of Aegilops tauschii from five countries was assessed using sequence-tagged microsatellites (or simple sequence repeats, SSRs) and amplified fragment length polymorphisms (AFLPs). In the case of AFLP analysis, a total of 256 amplification products obtained, 234 of them were polymorphic across all the 54 accessions. A total of 224 fragments were obtained from the 24 SSR primers and 219 of fragments were polymorphic across all the genotypes screened. Based on both AFLP and SSR markers, the highest percentage of polymorphisms were obtained in Iranian and accessions of unknown origin. The highest polymorphic information content (PIC) value was observed for SSRs (0.82) while the highest marker index (MI) value was for AFLPs (8.5) reflecting the hyper-variability of the first and the distinctive nature of the second system. Principal co-ordinate analysis (PCO) revealed congruent patterns of genetic relationships for both data sets, but did not group accessions strictly according to their geographical origins. Poor correlation was found between AFLP and SSR marker loci. This low association may be due to low number of AFLP and SSR markers. These results show that molecular markers can help to organize the genetic variability and expose useful diversity for breeding purposes.