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281.
Role of LBPA and Alix in multivesicular liposome formation and endosome organization 总被引:1,自引:0,他引:1
Matsuo H Chevallier J Mayran N Le Blanc I Ferguson C Fauré J Blanc NS Matile S Dubochet J Sadoul R Parton RG Vilbois F Gruenberg J 《Science (New York, N.Y.)》2004,303(5657):531-534
What are the components that control the assembly of subcellular organelles in eukaryotic cells? Although membranes can clearly be distorted by cytosolic factors, very little is known about the intrinsic mechanisms that control the biogenesis, shape, and organization of organellar membranes. Here, we found that the unconventional phospholipid lysobisphosphatidic acid (LBPA) could induce the formation of multivesicular liposomes that resembled the multivesicular endosomes that exist where this lipid is found in vivo. This process depended on the same pH gradient that exists across endosome membranes in vivo and was selectively controlled by Alix. In turn, Alix regulated the organization of LBPA-containing endosomes in vivo. 相似文献
282.
283.
Gobert V Gottar M Matskevich AA Rutschmann S Royet J Belvin M Hoffmann JA Ferrandon D 《Science (New York, N.Y.)》2003,302(5653):2126-2130
The Toll-dependent defense against Gram-positive bacterial infections in Drosophila is mediated through the peptidoglycan recognition protein SA (PGRP-SA). A mutation termed osiris disrupts the Gram-negative binding protein 1 (GNBP1) gene and leads to compromised survival of mutant flies after Gram-positive infections, but not after fungal or Gram-negative bacterial challenge. Our results demonstrate that GNBP1 and PGRP-SA can jointly activate the Toll pathway. The potential for a combination of distinct proteins to mediate detection of infectious nonself in the fly will refine the concept of pattern recognition in insects. 相似文献
284.
The development of a coupled model (PCPF-SWMS) to simulate water flow and pollutant transport in Japanese paddy fields 总被引:1,自引:0,他引:1
Julien Tournebize Hirozumi Watanabe Kazuhiro Takagi Taku Nishimura 《Paddy and Water Environment》2006,4(1):39-51
A new coupled model (PCPF–SWMS) was developed for simulating fate and behavior of pollutant in paddy water and paddy soil.
The model coupled the PCPF-1, a lumped model simulating pesticide concentrations in paddy water and 1 cm-surface sediment
compartment, and the SWMS-2D, a finite element numerical model solving Richard's and advection-dispersion equations for solute
transport in soil compartment. The coupling involved improvements on interactions of the water flow and the concentration
the pollutant of at the soil interface between both compartments. The monitoring data collected from experimental plots in
Tsukuba, Japan in 1998 and 1999 were used to parameterise and calibrate hydraulic functioning, hydrodynamic and hydrodispersive
parameters of the paddy soil. The analysis on the hydraulic functioning of paddy soil revealed that the hard pan layer was
the key factor controlling percolation rate and tracer transport. Matric potential and tracer monitoring highlighted the evolution
of saturated hydraulic conductivity (K
S) of hard pan layer during the crop season. K
S slightly decreased after puddling by clay clogging and strongly increased after mid term drainage by drying cracks. The model
was able to calculate residential time in every soil layers. Residential time of tracer in top saturated layers was evaluated
to be less than 40 days. It took 60 days to reach the unsaturated layers below hardpan layer. 相似文献
285.
We apply decision theory in a structured decision-making framework to evaluate how control of raccoons (Procyon lotor), a native predator, can promote the conservation of a declining population of American Oystercatchers (Haematopus palliatus) on the Outer Banks of North Carolina. Our management objective was to maintain Oystercatcher productivity above a level deemed necessary for population recovery while minimizing raccoon removal. We evaluated several scenarios including no raccoon removal, and applied an adaptive optimization algorithm to account for parameter uncertainty. We show how adaptive optimization can be used to account for uncertainties about how raccoon control may affect Oystercatcher productivity. Adaptive management can reduce this type of uncertainty and is particularly well suited for addressing controversial management issues such as native predator control. The case study also offers several insights that may be relevant to the optimal control of other native predators. First, we found that stage-specific removal policies (e.g., yearling versus adult raccoon removals) were most efficient if the reproductive values among stage classes were very different. Second, we found that the optimal control of raccoons would result in higher Oystercatcher productivity than the minimum levels recommended for this species. Third, we found that removing more raccoons initially minimized the total number of removals necessary to meet long term management objectives. Finally, if for logistical reasons managers cannot sustain a removal program by removing a minimum number of raccoons annually, managers may run the risk of creating an ecological trap for Oystercatchers. 相似文献