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61.
The integron content of 52 DT104/U302 phage type strains and 53 non-DT104/U302 strains of Salmonella enterica serotype Typhimurium (S. Typhimurium) was studied in PCR experiments using a 5'-CS/3'-CS primer pair (Lévesque et al., 1995). Forty-three out of 44 streptomycin- and/or ampicillin-resistant DT104 and related phage type strains were found to carry a 1 kb and/or 1.2 kb long integron. The other resistance markers did not affect the number and size of integrons; no integron-free multidrug-resistant (MDR) DT104 strains were found. The two large groups of DT104 strains (Felix-Callow's phage types 2 and 2c) proved to be identical in respect of integron patterns (IPs), supporting the views of those authors who consider DT104 a single clone. Strains of human and animal origin did not differ from each other in their IPs. Within the non-DT104 phage types, ampicillin- and/or streptomycin-resistant, integron-free MDR strains were also found. Based on amplicons varying between 290 and 3500 bp an IP system was suggested. The commonest amplicon sizes in non-DT104 strains were 1450 and 2050 bp. The IPs of DT104 strains and of non-DT104 strains containing an integron of 1 and 1.2 kb size were stable. In contrast, the IPs of other non-DT104 strains showed a varying degree of instability. Integron loss was frequently associated with spontaneous plasmid elimination and changes of R-type among the descendants of a given strain. 相似文献
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Bene Szabolcs Szabó Ferenc Polgár J. Péter Juhász Judit Nagy Péter 《Tropical animal health and production》2020,52(5):2333-2340
Tropical Animal Health and Production - Birth weight data of dromedary calves from the database of one of the world’s largest dairy herds (Dubai, UAE) were analyzed for the period from 2007... 相似文献
63.
During the last few years following the invasion of European tomato crops by the invasive South American tomato leafminer, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), several indigenous larval parasitoid species were recorded as natural enemies of this pest. Necremnus cf. artynes (Walker) and to a lesser extent Stenomesius sp. nr. japonicus (Ashmead) (Hymenoptera: Eulophidae) have been frequently reported attacking T. absoluta in tomato greenhouses in Europe and they are considered as possible biocontrol agents. Few biological data are available on these two species, notably when they parasitize T. absoluta. Therefore, the present study documents their biology when parasitizing T. absoluta, and provides scientific bases for potential development of biological control programs relying on larval ectoparasitoids in invaded areas. We demonstrated the ability of the two parasitoid species to reproduce on T. absoluta as host on tomato plants. On average, N. cf. artynes female parasitized 28.5 larvae and killed 26.2 larvae during its life time whereas S. sp. nr. japonicus female was more long-lived and consequently more efficient; it parasitized 144.3 larvae and killed 90.1 larvae. In a second step, we also explored the effect of T. absoluta larvae instar on parasitism by S. sp. nr. japonicus and subsequent development of the parasitoid offspring. Best results were obtained with the third larvae instar with a higher number of offspring produced, bigger individuals and a higher proportion of females. Our results support the hypothesis that the two species are good candidates for biological control programs targeting T. absoluta in Europe. 相似文献
64.
Magdalena L.Blum Judit Szonyi 《农业科学与技术》2014,(7):552-562
The study reflects on previous World Bank and FAO reports that made the general recommendation to set both research and extension investment targets in developing countries at 1% of agricultural gross domestic product (AgGDP). In order to define proxies for country-specific extension investment targets, authors developed an extension investment model (EIM) based on socio-economic macro-indicators (poverty, undernourishment, access to information and population density) and a method to define estimates for cost increases related to climate change. These parameters helped estimating the demand for agricultural extension and investments required for it. Results showed that about half of the 94 developing and emerging countries should spend more than 1% of their respective share of GDP derived from agriculture and about a quarter of the countries, mostly in Africa and South East Asia, need to spend more than 2% of their AgGDP. The paper reveals significant differences in average investment requirements in different regions and shows the additional extension costs related to climate change and other areas that currently lack investment. 相似文献
65.
Mezey E Nagy A Szalayova I Key S Bratincsak A Baffi J Shahar T 《Science (New York, N.Y.)》2003,299(5610):1184; author reply 1184
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We studied population extinction of the locally abundant fen plant Swertia perennis in Switzerland and used up to 127-year old herbarium records to relocate 63 sites that had once hosted this species. We recorded current site characteristics and related them to the absence or abundance of populations. Fifty-four sites (86%) were still traditionally used (extensively mown or grazed). Fifteen populations (24%) had gone extinct. Extinction was more likely at lower altitude, in the peripheral distribution range of S. perennis (58% peripheral, 9% central populations extinct), on the smallest fens (75% extinct on fens <400 m2) and on fens with intensified land use. However, even on traditionally managed wetlands 18.5% of the populations had gone extinct. Moreover, 40% of all remaining populations were smaller than 250 flowering plants. We conclude that both intensified agricultural practice and habitat fragmentation contributed to local extinction of S. perennis. Small populations, especially, may not be able to persist in the long term. 相似文献