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Antimicrobial and antiplasmid activities of essential oils 总被引:7,自引:0,他引:7
The antimicrobial and antiplasmid activities of essential oils (orange oil, eucalyptus oil, fennel oil, geranium oil, juniper oil, peppermint oil, rosemary oil, purified turpentine oil, thyme oil, Australian tea tree oil) and of menthol, the main component of peppermint oil, were investigated. The antimicrobial activities were determined on the Gram (+) Staphylococcus epidermidis and the Gram (-) Escherichia coli F'lac K12 LE140, and on two yeast Saccharomyces cerevisiae 0425 delta/1 and 0425 52C strains. The antiplasmid activities were investigated on E. coli F'lac bacterial strain. Each of the oils exhibited antimicrobial activity and three of them antiplasmid action. The interaction of peppermint oil and menthol with the antibiotics was studied on the same bacterial strain with the checkerboard method. Peppermint oil and menthol displayed additive synergy with oxytetracycline. A new mechanism of plasmid curing was established for one of the oil components. 相似文献
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Lemos SG Nogueira AR Torre-Neto A Parra A Artigas J Alonso J 《Journal of agricultural and food chemistry》2004,52(19):5810-5815
A potentiometric sensor system based on potassium ion-selective electrodes was developed for agricultural purposes. Sensors were built using PVC ion-selective membranes over an inner solid contact prepared with graphite-epoxy composites. A copper plate was used as a reference electrode. A two-stage electronic circuit composed of current and voltage amplifiers was designed to interface the sensors to a distributed data acquisition system. Three ion-selective sensors and three off-the-shelf temperature sensors and their associated circuits were mounted in a PVC tube to set up a soil probe. The electronic controls were placed in an airtight box fixed at the upper part of the probe. The system was evaluated in the field, where the sensors presented sensibility within the range of 69-71 mV dec(-)(1). Extracts of soil samples were analyzed by a current flame photometry approach, and the results, compared with the probe measurements, showed a linear relationship (r (2) = 0.992 and 0.995, respectively, to 5 and 20 cm depths), which implies viability and instrumentation reliability for agricultural applications. 相似文献
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Bryan HM Darimont CT Paquet PC Ellis JA Goji N Gouix M Smits JE 《Canadian journal of veterinary research》2011,75(1):11-17
Ranked among the top threats to conservation worldwide, infectious disease is of particular concern for wild canids because domestic dogs (Canis familiaris) may serve as sources and reservoirs of infection. On British Columbia's largely undeveloped but rapidly changing central and north coasts, little is known about diseases in wolves (Canis lupus) or other wildlife. However, several threats exist for transfer of diseases among unvaccinated dogs and wolves. To gain baseline data on infectious agents in this area, including those with zoonotic potential, we collected blood and stool samples from 107 dogs in 5 remote communities in May and September 2007. Serology revealed that the dogs had been exposed to canine parvovirus, canine distemper virus, Bordetella bronchiseptica, canine respiratory coronavirus, and Leptospira interrogans. No dogs showed evidence of exposure to Ehrlichia canis, Anaplasma phagocytophilum, Borrelia burgdorferi, Dirofilaria immitis, or Cryptococcus gattii. Of 75 stool samples, 31 contained at least 1 parasitic infection, including Taeniid tapeworms, the nematodes Toxocara canis and Toxascaris leonina, and the protozoans Isospora sp., Giardia sp., Cryptosporidium sp., and Sarcocystis sp. This work provides a sound baseline for future monitoring of infectious agents that could affect dogs, sympatric wild canids, other wildlife, and humans. 相似文献
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Márta Dobroné Tóth Judit L. Halász Sándor Balázsy 《Archives of Agronomy and Soil Science》2013,59(2):217-231
During growing and flowering seasons for five years, comparisons were made between upper and lower leaf surfaces microflora of ragweed plants grown in three areas: a relatively non-contaminated site, a communal landfill, and a complex contaminated with heavy metals. Results indicated that phyllospheric microbial communities were qualitatively and quantitatively related to metal content in plant leaf, leaf surfaces and metal contaminated ecosystems where the plants grown. Results showed that there was a high positive correlation between phyllospheric microbial population densities and Cd, Ni, and Zn content in ragweed leaves, while it was low in presence of Cu. Comparatively, the correlation was at its highest in the case of aerobic heterotrophic bacterial population densities and more than yeasts and filamentous fungi communities. The most common diversities of metal resistant microorganisms were related to Pantoea agglomerans, Pseudomonas savastanoi, P. putida, Bacillus cereus, Corynebacterium striatum, Cryptococcus and Alternaria. 相似文献
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Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling 总被引:1,自引:0,他引:1
Yu Y Yoon SO Poulogiannis G Yang Q Ma XM Villén J Kubica N Hoffman GR Cantley LC Gygi SP Blenis J 《Science (New York, N.Y.)》2011,332(6035):1322-1326
The evolutionarily conserved serine-threonine kinase mammalian target of rapamycin (mTOR) plays a critical role in regulating many pathophysiological processes. Functional characterization of the mTOR signaling pathways, however, has been hampered by the paucity of known substrates. We used large-scale quantitative phosphoproteomics experiments to define the signaling networks downstream of mTORC1 and mTORC2. Characterization of one mTORC1 substrate, the growth factor receptor-bound protein 10 (Grb10), showed that mTORC1-mediated phosphorylation stabilized Grb10, leading to feedback inhibition of the phosphatidylinositol 3-kinase (PI3K) and extracellular signal-regulated, mitogen-activated protein kinase (ERK-MAPK) pathways. Grb10 expression is frequently down-regulated in various cancers, and loss of Grb10 and loss of the well-established tumor suppressor phosphatase PTEN appear to be mutually exclusive events, suggesting that Grb10 might be a tumor suppressor regulated by mTORC1. 相似文献
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Bioassay-guided fractionation of the chloroform extract of Centaurea jacea L. afforded the isolation of cirsiliol, apigenin, hispidulin, eupatorin, isokaempferide, axillarin, centaureidin, 6-methoxykaempferol 3-methyl ether, trachelogenin, cnicin, 4'-acetylcnicin and three aliphatic glucose diesters, including the new natural product 1β-isobutanoyl-2-angeloyl-glucose. The structures of the compounds were established on the basis of spectroscopic analyses (UV, MS and NMR). All compounds were isolated for the first time from this species. The compounds were evaluated for their tumour cell growth inhibitory activities on HeLa, MCF-7 and A431 cells. Different types of secondary metabolites (flavonoids, sesquiterpenes) were found to be responsible for the antitumour effects of the extracts; the highest activity was exerted by centaureidin, in addition to moderately active compounds (cirsiliol, isokaempferide, apigenin, hispidulin, cnicin and 4'-acetylcnicin). 相似文献
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Delayed vaccine virus replication in chickens vaccinated subcutaneously with an immune complex infectious bursal disease vaccine: Quantification of vaccine virus by real-time polymerase chain reaction 下载免费PDF全文