Contour furrows for in situ soil and water conservation, Tigray, Northern Ethiopia

In Tigray (Northern Ethiopia), soil moisture has been identified as the most limiting factor in agricultural production; on the other hand, loss of rain water through runoff as well as the induced soil loss has been determined as a critical problem in the region in the last two to three decades. To alleviate the above paradox, the government has mobilized communities and resources for the construction of physical soil and water conservation structures (stone bunds, terraces) in almost all land uses. However, yield improvement was mainly concentrated within the vicinity of the structures and runoff continued to overtop the structures, as no measures for in situ soil conservation were taken. The terwah system, consisting of traditional ploughing followed by making every 1.5–2 m contour furrows, and permanent raised beds with contour furrows at 60–70 cm interval treatments, were considered and evaluated as practices that could increase the efficiency of in situ water utilization and soil conservation. An experiment was started in Gum Selasa, which is one of the drought prone areas in Tigray, whereby runoff volume and sediment load were measured after every rain event. Permanent raised beds with contour furrows at 60–70 cm interval significantly (P < 0.05) reduced runoff volume, runoff coefficient and soil loss as compared to traditional ploughing: 255, 381 and 653 m³ ha⁻¹ runoff was recorded from permanent bed, terwah and traditional ploughing, respectively during the whole cropping season. The above runoff induced 4.7 t ha⁻¹ soil loss from permanent bed, 7.6 t ha⁻¹ from terwah and 19.5 t ha⁻¹ from traditional ploughing. Overall, contour furrows and permanent raised beds can be part of the ongoing intensification process which includes physical soil and water conservation, slope reforestation, irrigation development and agro forestry in crop lands. Moreover, the use of permanent raised beds if combined with crop mulching and crop diversification is an important component for the development of sustainable conservation agriculture practices in the region.     

Generation of equine TSLP-specific antibodies and their use for detection of TSLP produced by equine keratinocytes and leukocytes

Allergic horses react to innocuous environmental substances by activation of Th2 cells and production of allergen-specific IgE antibodies. The mechanisms leading to Th2 differentiation are not well understood. In humans and mice, epithelial cell-derived thymic stromal lymphopoietin (TSLP) plays a central role in this process. Little is known about equine TSLP (eqTSLP) and its role in allergic diseases and our current knowledge is limited to the assessment of TSLP mRNA expression. In order to be able to study eqTSLP at the protein level, the aim of the present study was to produce recombinant eqTSLP protein and generate TSLP-specific antibodies. EqTSLP was cloned from a skin biopsy sample from a horse with chronic urticaria and eqTSLP protein was expressed in E.coli and in mammalian cells. Recombinant proteins were designed to include C-terminal Histag, which allowed subsequent purification and detection by Histag-specific Ab. Polyclonal and monoclonal eqTSLP-specific Ab were generated after immunization of mice with E.coli-expressed TSLP. Their specificity was tested by western blotting and ELISA. In addition, a commercially available polyclonal human TSLP-specific antibody was tested for cross-reactivity with eqTSLP. Expression of TSLP protein was confirmed by western blotting using Histag-specific Ab. E.coli-expressed TSLP appears as a band of ～13 kDa, whereas mammalian cell-expressed TSLP showed several bands at 20-25 kDa, probably representing several glycosylation forms. Polyclonal and monoclonal antibodies generated in this study, as well as commercially available human TSLP-specific Ab reacted with both E.coli- and mammalian cell-expressed TSLP in western blotting and ELISA. A capture ELISA was established to quantitate TSLP in cell supernatants and validated using supernatants from primary equine keratinocytes and peripheral blood leukocytes (PBL). Increased TSLP concentrations were found after stimulation of keratinocytes with PMA+ionomycine and with Culicoides extract. Similarly, increased TSLP concentrations were detected in PBL after stimulation with ConA, Culicoides extract, or IgE cross-linking. In conclusion, recombinant TSLP proteins and TSLP-specific antibodies produced in this study will allow further studies of the role of TSLP in equine allergic diseases.     

Characterization of the inflammatory infiltrate and cytokine expression in the skin of horses with recurrent urticaria

Background – Recurrent urticaria (RU) is a common skin disease of horses, but little is known about its pathogenesis. Hypothesis/Objective – The aim of this study was to characterize the inflammatory cell infiltrate and cytokine expression pattern in the skin of horses with RU. Animals – Biopsies of lesional and nonlesional skin of horses with RU (n = 8) and of skin from healthy control horses (n = 8) were evaluated. Methods – The inflammatory cell infiltrate was analysed by routine histology. Immunohistochemistry was used to identify T cells (CD3), B cells (CD79), macrophages (MAC387) and mast cells (tryptase). Expression of T‐helper 2 cytokines (interleukins IL‐4, IL‐5 and IL‐13), a T‐helper 1 cytokine (interferon‐γ), IL‐4 receptor α and thymic stromal lymphopoietin was assessed by quantitative RT‐PCR. Results – In subepidermal lesional skin of RU‐affected horses, increased numbers of eosinophils (P ≤ 0.01), CD79‐positive (P ≤ 0.01), MAC387‐positive (P ≤ 0.01) and tryptase‐positive cells (P ≤ 0.05) were found compared with healthy horses. Subepidermal lesional skin of RU‐affected horses contained more eosinophils (P ≤ 0.05) and tryptase‐positive cells (P ≤ 0.05) compared with nonlesional skin. There was no significant difference in infiltrating cells between nonlesional skin and skin of healthy horses. Expression of IL‐4 (P ≤ 0.01), IL‐13 (P ≤ 0.05), thymic stromal lymphopoietin (P ≤ 0.05) and IL‐4 receptor α (P ≤ 0.05) was increased in lesional skin of RU‐affected horses compared with control horses. Expression of IL‐4 was higher (P ≤ 0.05) in lesional compared with nonlesional RU skin. Conclusions and clinical importance – Analysis of cytokine expression and inflammatory infiltrate suggests that T‐helper 2 cytokines, eosinophils, mast cells and presumptive macrophages play a role in the pathogenesis of equine RU.     

ParaCalc®--a novel tool to evaluate the economic importance of worm infections on the dairy farm

Subclinical infections with gastrointestinal nematodes and liver fluke are important causes of production losses in grazing cattle. Although there is an extensive compilation of literature describing the effect of these infections on animal performance, only a few attempts have been made to convert these production losses to an economic cost. Here, we propose a novel tool (ParaCalc(?)), available as a web-application, to provide herd-specific estimates of the costs of these infections on dairy farms. ParaCalc(?) is a deterministic spread-sheet model where results from diagnostic methods to monitor the helminth infection status on a herd and anthelmintic usage are used as input parameters. Default values are provided to describe the effects of the infections on production and the cost of these production losses, but the latter can be adapted to improve the herd-specificity of the cost estimate. After development, ParaCalc(?) was applied on input parameters that were available for 93 Belgian dairy herds. In addition, the tool was provided to 6 veterinarians and their user experiences were evaluated. The estimated median [25th-75th percentile] cost per year per cow was € 46 [29-58] and € 6 [0-19] for gastrointestinal nematode and liver fluke infection, respectively. For both infections, the major components in the total costs were those associated with milk production losses in the adult cows. The veterinarians evaluated ParaCalc(?) as a useful tool to raise the farmers' awareness on the costs of worm infections, providing added value for their services. However, the score given for user-friendliness was diverse among users. Although the model behind ParaCalc(?) is a strong simplification of the real herd processes inducing economic losses, the tool may be used in the future to support economic decisions on helminth control.     

A survey to determine relationships between bulk tank milk antibodies against Ostertagia ostertagi and milk production parameters

In temperate climate regions, gastrointestinal nematodes are still widespread in adult dairy cows, but until now there exists no reliable diagnostic tool that can identify herds where the infection interferes with productivity. The objective of this study was to investigate the relationships between levels of antibodies against Ostertagia ostertagi in bulk tank milk and milk production. Bulk tank milk samples of 2553 dairy herds were obtained in spring and 2104 of these herds were sampled a second time in autumn. The antibody levels against O. ostertagi were determined with a milk ELISA and test results were expressed as an optical density ratio (ODR). The effect of bulk tank milk ODR on three different production parameters, kg milk, % and kg fat, % and kg protein was assessed by a multivariable linear regression model on the herds for which production data were available (n = 1063 and 867 in spring and autumn, respectively). The mean and standard deviation for ODRautumn (0.972+/-0.238) were higher than for ODRspring (0.825+/-0.201). Significant negative relationships were found between ODR and milk yield. An increase in ODRspring and ODRautumn from the 25th to the 75th percentile of the available ODR data was associated with a drop in the annual milk yield of 1.1 kg/cow/day, respectively 0.9 kg/cow/day. When a herd's ODR increased between spring and autumn with 0.142, it produced on average 0.4 kg/cow/day less in September than in April, in comparison with herds where the ODR did not increase. A significant negative association was found between ODRautumn and % protein averaged over the period of a year. No significant associations were found between ODR and % fat averaged over the year. When protein and fat production of September were expressed in kg an increase in ODRautumn from the 25th to the 75th percentile was associated with a decrease of 0.037 kg protein/cow/day and 0.042 kg fat/cow/day.     

Evaluation of three strategic parasite control programs in captive wild ruminants.

Parasite control in wild ruminants is based mainly on anthelmintics. The objective of the present study was to evaluate three parasite control programs in seven herds of captive wild ruminants in 3 consecutive years. In the first year, a biannual spring-summer treatment regime with fenbendazole at 7.5 mg/kg body weight p.o. for 3 days was applied. The next year, an early-season treatment program with three administrations of fenbendazole at the same dosage at 3-wk intervals was used. In the third year, an early-season treatment program with ivermectin (0.2 mg/kg p.o. for 3 days), applied three times at 5-wk intervals, was evaluated. Effectiveness of these control programs was assessed by fecal egg counts and by scores of body condition and fecal consistency at weekly intervals. Involved animal species were Arabian oryx (Oryx leucoryx), scimitar-horned oryx (Oryx dammah), slender-horned gazelle (Gazella leptoceros), Soay sheep (Ovis aries soay), ibex (Capra ibex), red deer (Cervus elaphus hippelaphus), and Nelson's elk (Cervus elaphus nelsoni). With the spring-summer regime, fecal egg counts remained low during the first 5 mo, but from September onward, they slowly increased to significant levels in all seven herds. The early-season program with fenbendazole resulted in fecal egg counts near zero during the entire year in four herds but a significant egg shedding from August onward was noted in Arabian oryx, scimitar-horned oryx, and Soay sheep. The early-season program with ivermectin resulted in very low to zero egg shedding in gazelle, adult Soay sheep, ibex, red deer, and Nelson's elk during the entire grazing season, but failed to prevent high shedding in October in Arabian oryx and scimitar-horned oryx. High contamination of grassy pastures with infective larvae/eggs in the previous year or before the first treatment may explain failure of the treatment regimes in some herds.     

The effect of short-term socio-economic and demographic change on landuse dynamics and its corresponding geomorphic response with relation to water erosion in a tropical mountainous catchment,Ecuador

The analysis of aerial photographs over a 33-year period (1962–1995) shows that land use in the study catchment is highly dynamic as a response to the land reform programs of the 1960s and 1970s and a strong population increase. The secondary forest is increasingly replaced by grassland while old grasslands are now used as cultivated land. Despite the increased pressure on the land, the upward movement of agricultural activity and the concurrent deforestation, the overall forest cover did not decline. The deforestation in the uplands is compensated for by a regeneration of secondary forest on abandoned rangelands and afforestation with Eucalyptus trees in the low-lying areas. The land use changes resulted in a strong decrease of the areas subject to intense soil degradation: afforestation with Eucalyptus trees on degraded lands was successful in controlling soil erosion in the lower parts of the catchment. The relationship between land use and sediment load in the river system is not straightforward. Statistical analysis of a time series of suspended sediment concentrations, which were measured at the outlet of three distinctive sub-catchments for a six-year period (1994 – 2000), revealed that the geomorphic response of the river system is not only dependent on the land use and the area affected by water erosion, but also on the spatial connectivity between sediment producing areas and the river network.This revised version was published online in May 2005 with corrections to the Cover Date.     

Influence of amylovoran production on virulence of Erwinia amylovora and a different amylovoran structure in E. amylovora isolates from Rubus

The amylovoran structures of five Erwinia amylovora isolates from Malaceae sp. and four isolates from Rubus sp. host plants were fully established, mainly by NMR. The structural data on one E. amylovora isolate from a Malaceae sp. host, which had been previously suggested by mass and NMR (Nimtz et al., 1996), were completed. E. amylovora strains infective on Malaceae sp. host plants had an amylovoran composed of pentasaccharide and 30–40% hexasaccharide repeating-substructures, whereas amylovoran from E. amylovora isolates from Rubus sp. host plants had only the pentasaccharide substructures. On the other hand, the exopolysaccharide (EPS) production differed in wild-type E. amylovora strains. Data on in vitro amylovoran production per cell could account for the differences in aggressiveness found in E. amylovora strains, as deduced from a pilot test with highly, moderately, and weakly aggressive strains. This correlation was confirmed with several other wild-type E. amylovora strains from different origin.