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971.
You Q Verschoor CP Pant SD Macri J Kirby GM Karrow NA 《Veterinary immunology and immunopathology》2012,148(3-4):243-251
Johne's disease (JD) is a widespread and economically important chronic inflammatory disease of the small intestine of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Although there are several techniques available for diagnosis of JD, their sensitivity is questionable. New proteome profiling methods, such as serum/plasma protein fingerprinting by 2-Dimensional Fluorescence Difference Gel Electrophoresis (2D-DIGE), may therefore be useful for identifying novel protein biomarkers of MAP infection. In this study, plasma samples were collected from 380 Holstein cows and screened for the presence of MAP infection using the M.pt. Johne's antibody Kit (IDEXX). Five negative (MAP-), and 5 strongly positive (MAP+) cows were selected for proteomic analysis. Highly abundant proteins were depleted from the plasma samples using the ProteoMiner technology (Bio-Rad) to enhance the resolution of low abundance proteins. Plasma samples from MAP-, MAP+, and a pooled internal control were labelled with different fluorescent dyes and separated based on their isoelectrical point (IP) and then their molecular weight. Gel images of the fluorescent plasma protein maps were acquired using a Typhoon scanner and analyzed using the DeCyder software. Proteins that were differentially expressed were excised from the gels, trypsin digested, and subjected to MS/MS analysis for identification. Six proteins were identified as being up-regulated at least 2-fold in MAP+ cows including: transferrin, gelsolin isoforms α & β (actin binding protein - ABP), complement subcomponent C1r, complement component C3, amine oxidase - copper containing 3 (AOC3), and coagulation factor II (thrombin) (p<0.05). Two proteins that were down-regulated approximately 2-fold in the MAP+ cows included coagulation factor XIII -B polypeptide (COAFXIII), and fibrinogen γ chain (FGG) and its precursor. 相似文献
972.
973.
974.
The purpose of this study was to compare half-Fourier-acquisition single-shot turbo spin-echo (HASTE) and T2-weighted (T2-W) sequences in dogs with thoracolumbar disc extrusion. MRI studies in 60 dogs (767 individual intervertebral disc spaces) were evaluated. Agreement between T2-W and HASTE sequences was assessed for two criteria: presence of an extradural lesion and treatment recommendation. There was moderate agreement between T2-W and HASTE sequences as to presence of an extradural lesion (kappa = 0.575). HASTE was in agreement in 96.1% of the sites where no extradural lesion was identified on T2-W images, but only in 58.1% of the sites where extradural lesions were identified on T2-W images. There was also moderate agreement between T2-W and HASTE sequences as to treatment recommendations (kappa = 0.476). HASTE was in agreement in 98.4% of the sites where a lesion was considered nonsurgical on T2 but only 82.1% of sites a lesion was considered surgical on T2. In 1.0% of sites considered not surgical and in 9.8% of sites considered equivocal based on T2-W images, a surgical lesion was identified on HASTE. Acquisition of a HASTE sequence in addition to conventional sequences may be beneficial in determining the severity of spinal cord compression in some cases when evaluating the canine spine. 相似文献
975.
Pelosi A Prinsen JK Eyster GE Schall W Olivier NB 《Veterinary radiology & ultrasound》2012,53(3):233-235
A 9-year-old dog with spontaneous ascites was found to have hepatic vein distension and a tortuous vena cava on abdominal ultrasound. In right lateral recumbency, the caudal vena cava crossed the diaphragm and became kinked before entering into the right atrium. Following this observation, we performed an experimental study in a normal dog to determine whether kinking of the caudal vena cava could be the result and not the cause of ascites. Ascites was induced using warm saline injected through a needle inserted into the abdominal cavity. Venograms were collected from different body positions, under four conditions: before and after a total of one, two and 3 liters of saline had been injected. Caudal vena cava kinking was observed in the experimental dog after 2 liters of fluid had been injected. Vena cava obstruction may cause ascites, but we found that sometimes caudal vena cava kinking can be the result and not the cause of the peritoneal effusion. 相似文献
976.
Pam Dachung Luka Chrisostom Ayebazibwe David Shamaki Frank Norbert Mwiine Joseph Erume 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(3):323-325
Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives. 相似文献
977.
Guy B. Kyser Vanelle F. Peterson Josh S. Davy Joseph M. DiTomaso 《Strength and Conditioning Journal》2012,65(4):418-425
Medusahead (Taeniatherum caput-medusae [L.] Nevski), the most problematic invasive grass on many California rangelands, is difficult to control selectively in grasslands. Prescribed burning, grazing, and herbicides have been tested with some success but are not practical in all situations. The selective herbicide aminopyralid, normally used for control of certain broadleaf species such as thistles, suppresses some annual grasses when applied pre- or early postemergence. In 2009–2010, we tested the efficacy of aminopyralid for medusahead control in preemergence applications at three foothill rangeland sites in northern California. We compared a rate series of aminopyralid (53, 88, 123, and 245 g · ha-1 acid equivalent [ae]) with rimsulfuron (18 and 35 g · ha-1 active ingredient) and imazapic (140 g · ha-1 ae). Plots were 3 × 9 m with four replications at each site. Treatments were applied in early fall 2009. In May 2010, we took visual cover estimates and biomass/seedhead samples in three quadrats per plot. In regression analysis, medusahead cover was found to decrease consistently with increasing rates of aminopyralid. Medusahead control at the highest rates of aminopyralid was consistent across the three sites, averaging 89% ± 3 standard deviation (SD) with 245 g · ha-1 ae and 59% ± 10 SD with 123 g · ha-1 ae. Aminopyralid at lower rates, rimsulfuron, and imazapic were less consistent. Cover of other annual grasses increased in plots treated with aminopyralid at all sites. Aminopyralid has potential utility for suppressing medusahead, particularly in sites also infested with invasive members of the Asteraceae. However, the most effective rate (245 g · ha-1 ae) is registered for use only as a spot application. In situations where this rate can be justifiably used, it would be expected to give season-long control of medusahead, as well as longer-term control of thistles and other susceptible species. 相似文献
978.
Brian M. Zanghi Wendell Kerr Christina de Rivera Joseph A. Araujo Norton W. Milgram 《Journal of Veterinary Behavior: Clinical Applications and Research》2012,7(6):339-347
Dogs exhibit biorhythms like diurnal sleep/wake and rest/activity behavior patterns with circadian rhythmicity and, similar to other mammalian species, show age-dependent changes in activity rhythms. This study sought to further characterize night/day locomotor activity patterns in adult dogs of various ages, and to examine the effect of feeding frequency (once vs. twice). Three groups (early adults [EA], 1.5-4.5 years; late adults [LA], 7-9 years; senior, 11-14 years) of beagles (N = 48) wore the Actiwatch activity monitoring system for 3 days while housed indoors with 12-hour light/dark schedule. Activity recording devices were used to collect actigraphy data when dogs were on a once- or twice-daily feeding regimen. All dogs demonstrated a circadian activity pattern with high levels of daytime activity and low levels of nighttime activity. The main effect of age differed for both daytime (P < 0.0001) and nighttime (P = 0.002) activity counts. Daytime activity in senior dogs was 17% and 42% lower compared with LA and EA dogs, respectively. With nighttime activity, both LA and seniors had significantly lower counts compared with the EA. Compared to once-daily feeding, twice-daily feeding significantly increased night activity, night/day activity ratio, and activity counts 60 minutes before the light phase across all 3 age-groups. This study provides a comprehensive characterization of age-related changes in locomotor behavior patterns in dogs and uniquely demonstrates that frequency of feeding can influence nighttime activity levels, which is partially associated with level of prelight onset of activity. 相似文献
979.
Lutishoor Salisbury Abayomi Omotola Omolewu Jeremy Joseph Smith 《Journal Of Agricultural & Food Information》2017,18(2):136-144
Interlibrary loan requests in the fields of agricultural, food, and life sciences were analyzed by material type, department, and levels of users. These data were then used to identify the sources for “free” publications which would have satisfied these requests; to identify whether the “free” full-text found are available as.pdf,.html, or.doc documents, etc.; and to identify the top 30 sources from which the “free” items are available. Twenty-four percent of the requested items were found with full-text via Google Scholar. These came from several sources with ResearchGate, NIH.gov, several publishers, and.edu sites being the top ones. 相似文献
980.
Thi Thu Nhan Nguyen Helen M. Wallace Cheng-Yuan Xu Zhihong Xu Michael B. Farrar Stephen Joseph Lukas Van Zwieten Shahla Hosseini Bai 《Journal of Soils and Sediments》2017,17(12):2763-2774