The aim of this study was to evaluate the effect of daily biotin supplementation on the mineral composition and microstructure of the abaxial hoof wall in dairy heifers. The heifers were housed on a concrete floor and fed for weight gain more than 800 g per day, which is a challenging environment for the hoof. Twelve crossbred dairy heifers (Jersey × Holstein) were divided into two treatment groups. Animals in the control group (n = 6) received a diet without supplemental biotin, while the heifers in the biotin-supplemented feed group (n = 6) each received 20 mg of biotin daily for 120 days. Samples of the abaxial hoof wall were collected from the outer claw of the fore and hind limb, before and after supplementation. The samples were evaluated by X-ray fluorescence spectrometry, computed microtomography, atomic force microscopy and confocal laser scanning microscopy. Biotin supplementation increased the sulphur content and decreased the calcium and potassium content in the abaxial hoof wall. Biotin treatment also increased the percentage of horn tubules with smaller diameter marrow (17–51 µm). However, biotin did not influence the surface relief of the hoof wall, suggesting that its action is limited to the inner layers of the stratum corneum. Daily supplementation with 20 mg of biotin promoted changes in the mineral composition and microstructure of abaxial hoof wall of crossbred dairy heifers. These findings suggest biotin supplementation improves hoof quality and may help to understand the function of biotin in the stratum corneum. 相似文献
A dairy cattle simulation model for pastoral systems that considers how dairy cow genotypes respond to different environments is described. The dairy cow is represented by five modules for maintenance, pregnancy, growth, body energy reserves and lactation with the influence of environmental factors on processes included within each module. Feed intake is predicted based on the requirements for maintenance, growth and pregnancy, and the dairy cow’s potential for yields of milk, fat and protein and body fat change in a given environment. The effects of various temporary environmental factors such as cow body condition score, climate, feed quality and the stage of pregnancy are all considered when predicting yields of milk, fat and protein, energy and dry matter intake. The model was evaluated using information from a prior experimental study with 1990s Holstein-Friesian dairy cattle of North American/European or New Zealand origin managed in a pasture-based system in early to peak lactation. The model was able to predict, to a high degree of accuracy, mean values for yields of milk, fat and protein, and concentrations of fat and protein. However for individual cows, feed intake and live weight change were less reliably predicted. The major source of error was a lack of simulated variation, rather than any systematic bias. The major advance of the model is its ability to predict performance from genetic and environmental sensitivity information for particular breeds, and its ability to predict feed intake and yields of milk, fat and protein concurrently. 相似文献
We have established marker-aided selection strategies for the two major Rf genes (Rf3 and Rf4) governing fertility restoration of␣cytoplasmic-genetic male sterility (CMS) in rice. Polymorphisms between restorer and
non-restorer␣lines were observed using RG140/PvuII for Rf3 located on chromosome 1 and S10019/BstUI for Rf4 located on chromosome 10. DNA polymorphisms associated with these two loci in restorer lines of wild abortive (WA), Dissi, and Gambiaca cytoplasm are
conserved, suggesting that similar biological processes control pollen fertility in this diverse cytoplasm. Because of their
close linkage to Rf genes and distinct banding patterns, STS markers RG140/PvuII and S10019/BstUI are well suited for marker-aided selection, enhanced backcross procedures, and pyramiding of Rf genes in agronomically superior non-restorer lines. The combined use of markers associated with these two loci improved the
efficiency of screening for putative restorer lines from a set of elite lines. Positional analyses of Rf4 and the inheritance pattern of the polymorphism in S10019/BstUI suggest that Rf4, governing fertility restoration in WA-CMS in rice, is likely to be the same gene governing fertility restoration in BT-
and HL-CMS that has a gametophytic effect, which explains why 100% pollen fertility in hybrids is impossible to attain. 相似文献
The uptake, bioaccumulation, biotransformation, and risks of nanomaterials (NMs) for food crops are still not well understood. Very few NMs and plant species have been studied, mainly at the very early growth stages of the plants. Most of the studies, except one with multiwalled carbon nanotubes performed on the model plant Arabidopsis thaliana and another with ZnO nanoparticles (NPs) on ryegrass, reported the effect of NMs on seed germination or 15-day-old seedlings. Very few references describe the biotransformation of NMs in food crops, and the possible transmission of the NMs to the next generation of plants exposed to NMs is unknown. The possible biomagnification of NPs in the food chain is also unknown. 相似文献
BACKGROUND: Captan is an important fungicide for controlling diseases in horticultural crops. Understanding its dissipation is important for estimating dietary risks and optimising pesticide application. Field experiments were conducted on apple leaves and fruit to investigate (1) the temporal variability of captan residues, (2) the contribution of several factors to the variability in residues and (3) the relationship between residues and climatic conditions. RESULTS: Initial captan deposits and subsequent residues on fruit and leaves were closer to a lognormal than to a normal distribution. The unit-to-unit variation contributed most to the observed variability in the initial deposit and subsequent residues. Variability due to orchards or trees or tree-zone interactions was also frequently important, but there was no discernable trend in the effects. The variability in residues did not appear to decrease over time. Canopy structure affected greatly the initial deposition but had little direct effect on subsequent captan loss. Fruit and leaves on the outside of the tree canopy received more deposit than those on the inside, but these differences gradually decreased over time. Captan loss resulted mainly from the first rainfall after an application. CONCLUSIONS: Captan loss is mainly due to rain, and the loss is negligible under dry conditions. 相似文献
Early growth and physiology of longleaf pine (Pinus palustris Mill.) seedlings were studied in response to light, water and nitrogen under greenhouse conditions. The experiment was conducted with 1-year-old seedlings grown in 11.3 l pots. The experimental design was a split-plot factorial with two levels (low and high) of each of the factors, replicated in three blocks. The four factorial combinations of water and nitrogen were randomly applied to 15 pots (sub-plots) in each of the light treatment (main plot). Data were collected on survival, root collar diameter (RCD), and height on a monthly basis. Biomass (shoot, root and needle), leaf area index, specific needle area, and needle nutrient (N, P, K, Ca, and Mg) concentrations were determined following final harvest after 16 months. Physiological data (net photosynthesis and transpiration) were collected monthly from March to July during the second growing season.
Height and RCD were significantly influenced by nitrogen and water and by the interaction between them with no apparent effect of light. Seedlings grew 93% taller in the high nitrogen and well watered (HNWW) treatment compared to the low nitrogen and water stressed (LNWS) treatment. Similarly, a significant increase (78%) in RCD was observed for seedlings in the HNWW treatment over the LNWS treatment. Light, along with water and nitrogen, played an important role in seedling biomass growth, especially when water was not limiting. Biomass partitioning (as measured by root:shoot ratio) was affected only by nitrogen and water. Nutrient stress had a greater influence on carbon allocation (69% increase in root:shoot ratio) than water stress (19% increase). Net photosynthesis (Pnet) was significantly higher for seedlings in the high resource than in the low resource treatments with significant light×water and nitrogen×water interactions. Transpiration rate was higher (75%) under the WW treatment compared to the WS treatment. Longleaf pine seedlings grown under the LNWW treatment had the lowest foliar nitrogen (0.71%) whereas seedlings in the HNWS treatment had the highest (1.46%). Increasing the availability of light (through larger canopy openings or controlling midstory density) and soil nitrogen (through fertilization) may not result in greater Pnet and improved seedling growth unless soil water is not limiting. 相似文献
Understanding the belowground interactions between trees and crops is critical to successful management of agroforestry systems.
In a study of competition for water in an alley cropping system consisting of pecan (Carya illinoensis) and cotton (Gossypium hirsutum) in a sandy loam soil (Rhodic Paleudult) in Jay, Florida, root systems of the two species were separated by trenching to
120 cm depth. A polyethylene barrier was installed in half of the plots. Spatial and temporal variations in soil water content,
root distribution and water uptake by both species, and leaf area development and height of cotton were measured. Interspecific
competition for water was greater in the non-barrier treatment near tree rows than at the alley center. Competition became
evident 3 to 4 weeks after emergence of cotton and increased during the following 7 to 8 weeks. Compared with the non-barrier
treatment, the barrier treatment had higher soil water content and better growth of cotton (height, leaf area, and fine root
biomass). Cotton lint yield in the barrier treatment (677 kg ha–1) was similar to that in a sole-crop stand, but higher than in the non-barrier (502 kg ha–1) treatment. Lint production efficiency of plants was higher in the interior rows in the non-barrier treatment (0.197 kg lint
per square meter of leaf area, compared to 0.117 kg in the barrier treatment). The results suggest that trenching or even
deep disking parallel to the tree row may reduce competition for water, but the impact on tree growth cannot be established
from this study.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
This paper demonstrates the oscillatory characteristics of electrical signals acquired from two ornamental plant types (Epipremnum pinnatum and Philodendron scandens - Family Araceae), using a noninvasive acquisition system. The electrical signal was recorded using Ag/AgCl superficial electrodes inside a Faraday cage. The presence of the oscillatory electric generator was shown using a classical power spectral density. The Lempel and Ziv complexity measurement showed that the plant signal was not noise despite its nonlinear behavior. The oscillatory characteristics of the signal were explained using a simulated electrical model that establishes that for a frequency range from 5 to 15 Hz, the oscillatory characteristic is higher than for other frequency ranges. All results show that non-invasive electrical plant signals can be acquired with improvement of signal-to-noise ratio using a Faraday cage, and a simple electrical model is able to explain the electrical signal being generated. 相似文献