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41.
42.
Genetic variation of sweet potatoes (Ipomoea batatas L.) cultivated in Chile determined by RAPDs 总被引:1,自引:0,他引:1
Boris Sagredo Patricio Hinrichsen Horacio López Alberto Cubillos Carlos Muñoz 《Euphytica》1998,101(2):193-198
RAPD (randomly amplified polymorphic DNA) technology was applied to analyze the genetic variability of sweet potato germplasm
existing in Chile and elsewhere. Analysis of 28 cultivars from all over the world showed polymorphic bands with all 18 primers
tested. A total of 124 RAPD bands were scored with an average of 6.9 polymorphic bands per primer. These results confirm that
sweet potato exhibits high genetic variation. Two groups were distinguished: one containing Peruvian cultivars, and another
containing cultivars from the rest of the world. Analysis of 14 accessions from Central Chile and one from Northern Chile
showed polymorphic bands with 24 of 26 primers tested, but almost all of the 140 polymorphic bands merely showed the distinctness
of the Northern accession. The almost complete uniformity of the other 14 accessions shows that sweet potato germplasm collected
in Central Chile has very little genetic variability and may be derived from a single cultivar.
Based on these results and on historical records, some hypotheses are proposed to explain the origin of sweet potatoes cultivated
in Chile.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
43.
In a pear orchard, when leaf senescence occurs, nitrogen (N) is added to the soil by the fallen leaves and can be re-used by the tree after undergoing decomposition and mineralization processes. Studies on leaf decomposition and N mineralization in orchards are scarce but essential to understand the N balance in the tree–soil ecosystem in a sustainable or precision agriculture. This study aimed to quantify the contribution of pear tree senescent leaves to N cycling in the orchard and its re-cycling by the crop. ‘Rocha’ pear unlabelled leaves were incubated in situ using the litter-bag technique and 15N-enriched leaves were placed at the soil surface in undisturbed confined cores.One- to six-year-old pear trees returned to the soil between 1 kg N ha?1 year?1 and 6 kg N ha?1 year?1 from senescent leaves that decomposed at rates varying from 0.0025 day?1 (d?1) to 0.0047 d?1 (estimated by both techniques, respectively). In the litter-bags, after 506–641 days, only 18–35% of initial DW was recovered in the soil, whereas in the soil cores the weight loss was higher, resulting in only 30–6% of initial DW after 398–406 d. After this period, between 36% and 110% of the initial N of the senescent leaves was recovered as organic 15N in the surface soil layer (0–7.5 cm), depending on climatic conditions, and being more prone to be absorbed by weeds. 相似文献
44.
Sclerotinia sclerotiorum infections on sunflower capitula produce white rot, one of the most dangerous diseases sunflower bears in all humid areas.
Therefore, disease quantification on developed genetic materials is a very important action in sunflower breeding. Given the
horizontal type of resistance these evaluations should be made in different environments. Several hybrids obtained after crossing
seven female × seven male sunflower inbred lines in a factorial mating design were evaluated for resistance to white rot in
two locations in the southeast of the province of Buenos Aires, Argentina. Disease incidence and the symptom appearance index
indicated significant genotypic effects and genotype × environment (G × E) interactions. Genotypic effects were a greater
source of variance than the G × E interaction effects. The G × E interactions only represented changes in magnitude. These
results were used to identify the best genotypes for both resistance factors. Four hybrids showed low disease incidence values
under both artificial and natural infections, of which only two had high values of the symptom appearance index at both locations.
These hybrids are considered to have adequate resistance attributes for the ascosporic penetration and the mycelium extension
in the capitulum tissue and could therefore be sown in the province of Buenos Aires without increasing risks of S. sclerotiorum attacks. 相似文献
45.
Karina Janice Guadalupe Díaz-Resendiz Carlos Eduardo Covantes-Rosales Alma Betsaida Benítez-Trinidad Migdalia Sarahy Navidad-Murrieta Francisco Fabian Razura-Carmona Christian Daniel Carrillo-Cruz Edwin Jaime Frias-Delgadillo Daniela Alejandra Prez-Díaz Matxil Violeta Díaz-Benavides Mercedes Zambrano-Soria Guadalupe Herminia Ventura-Ramn Aurelio Romero-Castro David Alam-Escamilla Manuel Ivn Girn-Prez 《Marine drugs》2022,20(2)
Fucoidan is a polysaccharide obtained from marine brown algae, with anti-inflammatory, anti-viral, and immune-enhancing properties, thus, fucoidan may be used as an alternative treatment (complementary to prescribed medical therapy) for COVID-19 recovery. This work aimed to determine the ex-vivo effects of treatment with fucoidan (20 µg/mL) on mitochondrial membrane potential (ΔΨm, using a cationic cyanine dye, 3,3′-dihexyloxacarbocyanine iodide (DiOC6(3)) on human peripheral blood mononuclear cells (HPBMC) isolated from healthy control (HC) subjects, COVID-19 patients (C-19), and subjects that recently recovered from COVID-19 (R1, 40 ± 13 days after infection). In addition, ex-vivo treatment with fucoidan (20 and 50 µg/mL) was evaluated on ΔΨm loss induced by carbonyl cyanide 3-chlorophenylhydrazone (CCCP, 150 µM) in HPBMC isolated from healthy subjects (H) and recovered subjects at 11 months post-COVID-19 (R2, 335 ± 20 days after infection). Data indicate that SARS-CoV-2 infection induces HPBMC loss of ΔΨm, even 11 months after infection, however, fucoidan promotes recovery of ΔΨm in PBMCs from COVID-19 recovered subjects. Therefore, fucoidan may be a potential treatment to diminish long-term sequelae from COVID-19, using mitochondria as a therapeutic target for the recovery of cellular homeostasis. 相似文献
46.
Konaté K Souza A Coulibaly AY Meda NT Kiendrebeogo M Lamien-Meda A Millogo-Rasolodimby J Lamidi M Nacoulma OG 《Pakistan journal of biological sciences: PJBS》2010,13(22):1092-1098
In this study polyphenol content, antioxidant activity, lipoxygenase (LOX) and Xanthine Oxidase (XO) inhibitory effects of n-hexane, dichloromethane, ethyl acetate and n-butanol fractions of aqueous acetone extracts from S. alba L., S. acuta Burn f and Cienfuegosia digitata Cav. were investigated. The total phenolics, flavonoids, flavonols and total tannins were determined by spectrophotometric methods using Folin-ciocalteu, AlCl3 reagents and tannic acid, respectively. The antioxidant potential was evaluated using three methods: inhibition of free radical 2,2-diphenyl-1-picrylhydramzyl (DPPH), ABTS radical cation decolorization assay and Iron (III) to iron (II) reduction activity (FRAP). For enzymatic activity, lipoxygenase and xanthine oxidase inhibitory activities were used. This study shows a relationship between polyphenol contents, antioxidant and enzymatic activities. Present results showed that ethyl acetate and dichloromethane fractions elicit the highest polyphenol content, antioxidant and enzymatic activities. 相似文献
47.
Golovenko Ekaterina Lyashenko Svetlana Akimova Svetlana Mitina Lyubov Mulenkova Elena Belarbi El-Hassan Guil-Guerrero José Luis 《Plant foods for human nutrition (Dordrecht, Netherlands)》2021,76(3):385-393
Plant Foods for Human Nutrition - γ-linolenic acid (GLA, 18:3n-6) is a bioactive fatty acid (FA) that exerts several healthy actions; however, its occurrence is restricted to a few oils. The... 相似文献
48.
Luiz Henrique Agra Cavalcante-Silva Ana Carolina de Carvalho Correia José Maria Barbosa-Filho Bagnólia Araújo da Silva Bárbara Viviana de Oliveira Santos Daysianne Pereira de Lira Jéssica Celestino Ferreira Sousa George Emmanuel C. de Miranda Fabiana de Andrade Cavalcante Magna Suzana Alexandre-Moreira 《Marine drugs》2013,11(5):1553-1564
In this work, we investigated the spasmolytic effect of caulerpine, a bisindole alkaloid isolated from marine algae of the Caulerpa genus, on guinea pig ileum. Our findings indicated that caulerpine inhibited phasic contractions induced by carbachol (IC50 = 7.0 ± 1.9 × 10−5 M), histamine (IC50 = 1.3 ± 0.3 × 10−4 M) and serotonin (IC50 = 8.0 ± 1.4 × 10−5 M) in a non-selective manner. Furthermore, caulerpine concentration-dependently inhibited serotonin-induced cumulative contractions (pD′2 = 4.48 ± 0.08), shifting the curves to the right with Emax reduction and slope of 2.44 ± 0.21, suggesting a noncompetitive antagonism pseudo-irreversible. The alkaloid also relaxed the ileum pre-contracted by KCl (EC50 = 9.0 ± 0.9 × 10−5 M) and carbachol (EC50 = 4.6 ± 0.7 × 10−5 M) in a concentration-dependent manner. This effect was probably due to inhibition of Ca2+ influx through voltage-gated calcium channels (CaV), since caulerpine slightly inhibited the CaCl2-induced contractions in depolarizing medium without Ca2+, shifting the curves to the right and with Emax reduction. According to these results, the spasmolytic effect of caulerpine on guinea pig ileum seems to involve inhibition of Ca2+ influx through CaV. However, other mechanisms are not discarded. 相似文献
49.
Tatiana da Rosa Guimar?es Carlos Guillermo Quiroz Caroline Rigotto Simone Quintana de Oliveira Maria Tereza Rojo de Almeida éverson Miguel Bianco Maria Izabel Goulart Moritz Jo?o Luís Carraro Jorge Alejandro Palermo Gabriela Cabrera Eloir Paulo Schenkel Flávio Henrique Reginatto Cláudia Maria Oliveira Sim?es 《Marine drugs》2013,11(11):4176-4192
The n-butanol fraction (BF) obtained from the crude extract of the marine sponge Petromica citrina, the halistanol-enriched fraction (TSH fraction), and the isolated compounds halistanol sulfate (1) and halistanol sulfate C (2), were evaluated for their inhibitory effects on the replication of the Herpes Simplex Virus type 1 (HSV-1, KOS strain) by the viral plaque number reduction assay. The TSH fraction was the most effective against HSV-1 replication (SI = 15.33), whereas compounds 1 (SI = 2.46) and 2 (SI = 1.95) were less active. The most active fraction and these compounds were also assayed to determine the viral multiplication step(s) upon which they act as well as their potential synergistic effects. The anti-HSV-1 activity detected was mediated by the inhibition of virus attachment and by the penetration into Vero cells, the virucidal effect on virus particles, and by the impairment in levels of ICP27 and gD proteins of HSV-1. In summary, these results suggest that the anti-HSV-1 activity of TSH fraction detected is possibly related to the synergic effects of compounds 1 and 2. 相似文献
50.