Oesophageal strictures in cats associated with doxycycline therapy

Four cases of oesophageal stricture subsequent to doxycycline administration are reported. All cases were young to middle age (median age 3 years; range 1-7 years), and either domestic shorthair or domestic longhair breed. In all cases the predominant clinical sign was regurgitation, which developed at variable times after doxycycline administration. In all cases the reason for doxycycline use was treatment or prophylaxis of suspected infections (Mycoplasma haemofelis, Chlamydophila felis or Bordetella bronchiseptica), and the duration of therapy was variable. In one case the stricture was definitively diagnosed at post mortem examination, in the three other cases, definitive diagnosis was by endoscopy. Balloon dilation was successful in the three cases that were treated. This is the largest case series, to date, of oesophageal disease in cats associated with doxycycline administration. Caution should be exercised when administering oral medication to cats, especially doxycycline, and should be accompanied either by a water or food swallow.     

Effects of mass and position of artificial fouling added to the upper valve of the mangrove oyster Crassostrea rhizophorae on its growth and survival

We ran an experiment on mangrove oysters Crassostrea rhizophorae to evaluate the effects of adding different masses of artificial fouling to the upper valve, either to the umbo region or the ventral edge of the shell. Growth and survival were quantified after a 30 d period in suspended culture in the La Restinga Lagoon, Venezuela. The artificial fouling was cement weighing 0.5, 1, 2 or 3 fold the mass of the upper valve. No fouling was added to a control group. Fouling mass, but not the position of the artificial fouling, affected growth in shell length. However, only the heaviest fouling (3 times the mass of the upper valve) had a significant effect. In contrast, there was no affect of either fouling mass or position on tissue growth. Finally, our data indicated that mortality could be affected by the position where we added artificial fouling (greater mortality when fouling was added to the ventral edge of the shell), but not by fouling mass. Our study indicates it is unlikely that the levels of natural fouling that develop on oysters in suspended culture would be sufficient to affect either growth or survival.     

North American veterinary pathology residency training programs: an overview of where they are today and where they were five years ago, with an analysis of trends

An e-mail/telephone survey of all active North American residency training programs in veterinary pathology was conducted in September 2005. The purpose of this survey was to determine current numbers of trainees, their program length and type, and salaries; to compare current numbers to five years earlier; and, finally, to gauge interest in expanding current programs. All 41 training institutions contacted responded to the survey. Briefly, the survey found that there are currently 235 veterinary pathology residents, for a mean of 5.7 residents per training program. The number of residents currently in training programs and the number of applicants for these programs has increased compared to five years earlier. There is widespread interest in further expanding capacity in these programs, and the coalition of the American College of Veterinary Pathologists and the Society of Toxicologic Pathology is a well-known source of possible funding for additional residents. This survey report further documents the numbers of combined residency/PhD programs, average starting salaries for new residents, outside sponsorship effects on pathology training programs, and some of the common concerns regarding veterinary pathology training programs voiced by the respondents. While residency training capacity has expanded in the last five years, and there is widespread desire to further expand these training programs, a shortage of veterinary pathologists for future market needs will need to be addressed by increased funding from as yet unspecified sources.     

Mechanism of Na+/H+ antiporting

Na+/H+ antiporters are central to cellular salt and pH homeostasis. The structure of Escherichia coli NhaA was recently determined, but its mechanisms of transport and pH regulation remain elusive. We performed molecular dynamics simulations of NhaA that, with existing experimental data, enabled us to propose an atomically detailed model of antiporter function. Three conserved aspartates are key to our proposed mechanism: Asp164 (D164) is the Na+-binding site, D163 controls the alternating accessibility of this binding site to the cytoplasm or periplasm, and D133 is crucial for pH regulation. Consistent with experimental stoichiometry, two protons are required to transport a single Na+ ion: D163 protonates to reveal the Na+-binding site to the periplasm, and subsequent protonation of D164 releases Na+. Additional mutagenesis experiments further validated the model.     

Crop structure in winter oats and the effect of nitrogen on quality-related characters

The relationship between panicle structure and oat grain quality characteristics was examined in a winter oat (Avena sativa) experiment, cv. Gerald, fertilized with 80 and 200 kg/ha N. Spikelets on each whorl were counted and separated into primary, secondary, and tertiary grains, which were counted and weighed, and the kernels extracted manually and weighed. Tiller and whorl rank had relatively small effects on weight per grain and kernel content. Nitrogen application significantly increased weight per primary and secondary grain on the main stem and T1, the first tiller, while spikelet and grain numbers increased only on T1. However, weight per grain and kernel contents in all whorls on both the main stem and T1 were not significantly affected by nitrogen. Although kernel contents were significantly affected by nitrogen application on some occasions, increases and decreases were less than 2.5%. Across the whole plant, numbers of primary and secondary grains increased at the higher nitrogen application, but weight per primary grain and kernel contents were unaffected. However, weight per secondary grain significantly increased at the higher nitrogen application, thus improving uniformity of grain size which is of value to millers.     

The application of small unmanned aerial systems for precision agriculture: a review

Precision agriculture (PA) is the application of geospatial techniques and sensors (e.g., geographic information systems, remote sensing, GPS) to identify variations in the field and to deal with them using alternative strategies. In particular, high-resolution satellite imagery is now more commonly used to study these variations for crop and soil conditions. However, the availability and the often prohibitive costs of such imagery would suggest an alternative product for this particular application in PA. Specifically, images taken by low altitude remote sensing platforms, or small unmanned aerial systems (UAS), are shown to be a potential alternative given their low cost of operation in environmental monitoring, high spatial and temporal resolution, and their high flexibility in image acquisition programming. Not surprisingly, there have been several recent studies in the application of UAS imagery for PA. The results of these studies would indicate that, to provide a reliable end product to farmers, advances in platform design, production, standardization of image georeferencing and mosaicing, and information extraction workflow are required. Moreover, it is suggested that such endeavors should involve the farmer, particularly in the process of field design, image acquisition, image interpretation and analysis.     

应用137Cs示踪技术估算土壤侵蚀的若干问题探讨

[目的]探讨用~(137)Cs示踪技术估算土壤侵蚀量方法(以下简称"~(137)Cs示踪方法")所存在的几个关键问题,促进该方法的标准化和系统化。[方法]对大量已发表的相关文献进行分析,根据作者的研究经验,归纳出~(137)Cs示踪方法在实践应用中所存在的核心问题。[结果]~(137)Cs示踪方法中关于~(137)Cs在空间是均匀分布的假设存在不合理性,不能直接用于定量估算单钻点取样的土壤侵蚀量。~(137)Cs活度的空间变化存在随机性的成分。敏感度和不确定性分析结果证明~(137)Cs活度的空间随机变化量是~(137)Cs示踪方法不确性的最大来源。[结论]可以用多钻点样本平均值来减少~(137)Cs随机变化量所引起的侵蚀估算误差。以统计学为基础的试验设计和采取独立样本的办法可以消除该误差。虽然~(137)Cs模型已被广泛应用,但由于缺乏长期观测资料诸多模型还处在理论研究阶段,没有得到严格的验证和评判。因为不同模型估算的侵蚀量差别甚大,模型验证和筛选对该方法的成功运用至关重要。     

SNOMED representation of explanatory knowledge in veterinary clinical pathology

BACKGROUND: The Systematized Nomenclature of Medicine (SNOMED) is an established standard nomenclature for the expression of human and veterinary medical concepts. Nomenclature standards ease sharing of medical information, create common points of understanding, and improve data aggregation and analysis. OBJECTIVES: The objective of this study was to determine whether SNOMED adequately represented concepts relevant to veterinary clinical pathology. METHODS: Concepts were isolated from 3 different types of clinical pathology documents: 1) a textbook (Textbook), 2) the Results sections of industry pathology reports (Findings), and Discussion sections from industry pathology reports (Discussion). Concepts were matched (mapped) by 2 reviewers to semantically-equivalent SNOMED concepts. A quality score of 3 (good match), 2 (problem match), or 1 (no match) was recorded along with the SNOMED hierarchical location of each mapped concept. Results were analyzed using Cohen's Kappa statistic to assess reviewer agreement and chi-square tests to evaluate association between document type and quality score. RESULTS: The percentage of good matches was 48.3% for the Textbook, 45.4% for Findings, and 47.5% for Discussion documents, with no significant difference among documents. Of remaining concepts, 40% were partially expressed by SNOMED and 14% did not match. Mean reviewer agreement on quality score assignments was 76.8%. CONCLUSIONS: Although SNOMED representation of veterinary clinical pathology content was limited, missing and problem concepts were confined to a relatively small area of terminology. This limitation should be addressed in revisions of SNOMED to optimize SNOMED for veterinary clinical pathology applications.     

EXCRETORY UROGRAPHY BY INTRAOSSEOUS INJECTION OF CONTRAST MEDIA IN A RABBIT MODEL

There are many indications for an intravenous excretory urogram. However, where intravenous access is not available, the intraosseous route to the circulation may be an alternative. We found that safe and diagnostic excretory urograms could be obtained in rabbits following the injection of different contrast media via the intraosseous route. In fact, these excretory urograms were indistinguishable from ones obtained by the conventional intravenous route. While the rabbits did not develop any abnormal clinical signs following the procedure, there were postmortem histologic lesions of osteochondrosis in 5 of 22 (22.7%) tibias receiving an intraosseous needle, but in none of the 14 tibias that did not receive an intraosseous needle. Further, the use of diatrizoate was associated with the development of osteochondrosis while the use of iopamidol was not.