黑曲霉固体发酵产木聚糖酶的响应面优化设计及其酶学性质的研究

本研究采用响应面法的中心组合对影响黑曲霉(aspergillus niger JL 15)固体发酵产木聚糖酶的条件进行了优化.以橘皮粉为基质,补充碳源、氮源,以及含水量和发酵时间对木聚糖酶产量具有显著影响(P<0.05).模拟二次多项式回归预测模型,并建立自变量与响应值的回归方程,获得各因素的最佳水平,即:甘油、硫酸铵的添加量分别为4.2%和3.1%,含水量为61%,发酵时间为73.4 h,木聚糖酶活性最大预测值达922.9 U/g干发酵产物,验证值为917.7 U/g干发酵产物,高出基础培养基酶活3.2倍.酶学性质分析研究表明,黑曲霉木聚糖酶(XylA)的最适温度和最适pH分别为55oC和pH 5.0.XylA的K_m和V_(max)值分别为9.24 mg/mL和54.05 μmol/min/mL.Mn~(2+)、Zn~(2+)和斗和Mg~(2+)对XylA活性具有促进作用,而Fe~(3+)和cu~(2+)对XylA活性具有明显抑制作用.HPLC分析结果表明,XylA的水解桦木木聚糖和麸皮不溶性木聚糖的产物均为木糖至木六糖,主要产物为木三糖.     

Characteristics of Nitrogen Loss through Surface-Subsurface Flow on Red Soil Slopes of Southeast China

Soil nitrogen (N) loss related to surface flow and subsurface flow (including interflow and groundwater flow) from slope lands is a global issue. A lysimetric experiment with three types of land cover (grass cover, GC; litter cover, LC; and bare land, BL) were carried out on a red soil slope land in southeast China. Total Nitrogen (TN) loss through surface flow, interflow and groundwater flow was observed under 28 natural precipitation events from 2015 to 2016. TN concentrations from subsurface flow on BL and LC plots were, on average, 2.7–8.2 and 1.5–4.4 times greater than TN concentrations from surface flow, respectively; the average concentration of TN from subsurface flow on GC was about 36–56% of that recorded from surface flow. Surface flow, interflow and groundwater flow contributed 0–15, 2–9 and 76–96%, respectively, of loss load of TN. Compared with BL, GC and LC intercepted 83–86% of TN loss through surface runoff; GC intercepted 95% of TN loss through subsurface flow while TN loss through subsurface flow on LC is 2.3 times larger than that on BL. In conclusion, subsurface flow especially groundwater flow is the dominant hydrological rout for N loss that is usually underestimated. Grass cover has the high retention of N runoff loss while litter mulch will increase N leaching loss. These findings provide scientific support to control N runoff loss from the red soil slope lands by using suitable vegetation cover and mulching techniques.     

振动深松机多组振动深松铲自平衡性能及仿真分析

振动深松具有减阻的优势,但振动对拖拉机及驾驶员的不利影响制约其推广,该文对振动深松机多组振动深松铲进行自平衡性能分析。在优化试验中,多组深松铲振动作业时的剧烈振动易造成试验设备损坏,仿真试验可避免危险工作环境下的实车试验。对拖拉机-振动深松机系统进行受力分析,并基于ADAMS建立其仿真模型,建模过程包括导入三维模型、定义轮胎与地面之间接触力和摩擦力等。理论与仿真分析相互验证,得到拖拉机后轮所受支持力均值分别为27.8、26.4 kN,误差为1.4 kN,并且二者主振动曲线变化趋势一致。采用加权加速度均方根值评价振动对驾驶员的影响。通过MATLAB编程,利用功率谱密度函数,计算得到驾驶座质心总加权加速度均方根值。利用Design-Expert软件设计试验并优化得到6组振动影响较小的四组振动松土铲作业初始相位角组合,减振比率超过90%,实现了振动深松机作业时的自平衡。     

单轨道山地果园运输机齿条齿形优选

为减小单轨道山地果园运输机能耗及提高运输效率,该文基于动力学理论建立了运输机驱动轮与轨道齿条啮合的动力学模型,并设计、加工制造了链轮齿形齿条、销轮齿形齿条、摆线齿形齿条。以运输机驱动轮旋转角速度、轨道坡度、齿条齿形为考察因素,以驱动轮与不同齿形齿条啮合时所需提供的驱动扭矩为评价指标,探究齿条齿形对单轨道山地果园运输机力学性能的影响,得到在相同条件下驱动轮与链轮齿形齿条啮合时的驱动扭矩最小,且波动幅度最小。在驱动轮转速为+88.08 rad/s、轨道坡度分别为+0?、+6?、+12?时,驱动轮与链轮齿形齿条啮合时的驱动扭矩均值较驱动轮与圆弧齿形齿条啮合时的驱动扭矩均值分别减小33.82%,33.45%,21.59%;在驱动轮转速为-88.08 rad/s、轨道坡度分别为-0?、-6?、-12?时,驱动轮与链轮齿形齿条啮合时的驱动扭矩均值较驱动轮与圆弧齿形齿条啮合时的驱动扭矩均值分别减小35.55%,27.24%,30.43%。试验结果表明,链轮齿形齿条综合性能最优,较圆弧齿形齿条更适宜用于单轨道山地果园运输机的轨道运输中。该研究为单轨道山地果园运输机轨道的结构优化设计提供了参考。     

摩擦复充种型孔带式水稻精量排种器充种性能分析与验证

为解决杂交稻工厂化穴盘育秧低播量精密播种问题,提出了一种摩擦复充种型孔带式水稻精量排种器,对种子充填过程进行了力学和运动学分析,确定影响试验指标的影响因素分别为型孔方向角、型孔带速度、型孔带倾角和种层厚度。通过EDEM离散元软件仿真分析了种子多次循环重复充种过程和充种效果,并分别分析了型孔带速度、型孔带倾角、种层厚度对充种性能的影响规律。基于仿真分析结果进行了较优组合验证试验,结果表明:在型孔方向角为90°,型孔带倾角为43°,型孔带转速为0.11 m/s,种层厚度为50 mm时充种合格率为96.4%,多粒率为1.4%,漏充率为2.2%,种子破碎率为0.18%,效果较优。摩擦复充种型孔带式水稻精量排种器能够满足杂交稻低播量精密播种的农艺要求,研究结果为工厂化穴盘育秧精量播种机的设计提供参考。     

入射角度对气力射播小麦种粒入土参数影响的试验研究

针对稻麦轮作区黏湿土壤条件下的小麦机械化播种,为避免传统接触式小麦播种技术存在的粘附堵塞严重和作业阻力、功耗大等问题,该文采用非接触式小麦气力射播技术理论,通过自制的小麦气力射播试验台,开展整洁种床土壤条件下入射角度对气力射播小麦种粒入土参数影响的试验研究。试验结果表明:随着入射角度的增加,相同加速气压下小麦种粒的射播速度垂直分量和射播深度不断增加,小麦种粒触土后产生水平滑移和弹跳的几率变小,且各入射角度下射播深度与射播速度呈正相关关系,不同入射角度下小麦种粒呈现出不同射播深度和土壤冲击效果,小麦种粒未产生损坏情况;当入射角度≥45°和射播速度垂直分量≥25 m/s时,小麦种粒可被完全射播入土壤中且出苗率大于86%,90°入射角度下的射播效果最好。同时,为实现田间作业时小麦种粒的垂直射播入土,应使射播速度水平分量与机具作业速度相等且射播速度垂直分量≥25m/s。试验结果可为非接触式小麦射播装备的研制提供基础数据和技术支撑。     

Effects of part and whole straw returning on soil carbon sequestration in C3–C4 rotation cropland

Long‐term no‐tillage management and crop residue amendments to soil were identified as an effective measure to increase soil organic carbon (SOC). The SOC content, SOC stock (SOCs), soil carbon sequestration rate (CSR), and carbon pool management index (CPMI) were measured. A stable isotopic approach was used to evaluate the contributions of wheat and maize residues to SOC at a long‐term experimental site. We hypothesized that under no‐tillage conditions, straw retention quantity would affect soil carbon sequestration differently in surface and deep soil, and the contribution of C3 and C4 crops to soil carbon sequestration would be different. This study involved four maize straw returning treatments, which included no maize straw returning (NT‐0), 0.5 m (from the soil surface) maize straw returning (NT‐0.5), 1 m maize straw returning (NT‐1), and whole maize straw returning (NT‐W). The results showed that in the 0–20 cm soil layer, the SOC content, SOCs, CSR and CPMI of the NT‐W were highest after 14 years of no‐tillage management, and there were obvious differences among the four treatments. However, the SOC, SOCs, and CSR of the NT‐0.5 and NT‐W were the highest and lowest in 20–100 cm, respectively. The value of δ¹³C showed an obviously vertical variability that ranged from –22.01‰ (NT‐1) in the 0–20 cm layer to –18.27‰ (NT‐0.5) in the 60–80 cm layer, with enriched δ¹³C in the 60–80 cm (NT‐0.5 and NT‐1) and 80–100 cm (NT‐0 and NT‐W) layers. The contributions of the wheat and maize‐derived SOC of the NT‐0.5, NT‐1 and NT‐W increased by 11.4, 29.5 and 56.3% and by 10.7, 15.1 and 40.1%, relative to those in the NT‐0 treatment in the 0–20 cm soil layer, respectively. In conclusion, there was no apparent difference in total SOC sequestration between the NT‐0.5, NT‐1, and NT‐W treatments in the 0–100 cm soil layer. The contribution of wheat‐derived SOC was higher than that of maize‐derived SOC.     

河道淤泥和堆肥蛭石混合发酵制备基质及其育苗效果

农村河道清淤产生的淤泥,体量大、有机物浓度高,处置不当会造成二次污染。现代农业的工厂化育苗需求大量的营养土,就地取土导致耕地退化。该研究利用功能微生物发酵淤泥制备育苗基质,研究不同菌株发酵基质的物理和生物学性状,基质培育西瓜苗的生长、生理参数和抗逆性能。结果表明:微生物处理均能够提升淤泥基质物理和生物学性能,同时能够提升育苗质量。其中Trichoderma harzianum T83(T83)、Bacillus amyloliquefaciens IAE(BIAE)菌株发酵基质性能最好。相较于对照处理基质的最大持水量、总孔隙度、毛管孔隙度、通气孔隙度,T83处理分别增加了64.25%、52.65%、45.05%、56.11%;BIAE处理分别增加了101.17%、45.43%、61.43%、38.14%。相较于对照处理西瓜苗的株高、鲜质量、干质量、叶绿素含量、根系活力、根际真菌、细菌数量,T83处理分别增加了66.85%、52.07%、72.16%、43.13%、54.93%、110.62倍、1.63倍;BIAE处理分别增加了80.40%、57.34%、82.37%、54.88%、46.40%、67.26%、2.60倍、2.94倍。T83和BIAE处理西瓜苗叶片过氧化氢酶和超氧化物歧化酶酶活显著增加,根系丙二醛含量显著降低。真菌菌株T. harzianum T83和细菌菌株B. amyloliquefaciens IAE发酵淤泥,能够显著提升其农用品质,为淤泥高附加值化农用提供一条可行的途径。     

CT扫描分析秸秆隔层孔隙特征及其对土壤水入渗的影响

为阐明不同物理形态玉米秸秆建立的隔层对盐碱土壤灌溉水入渗特征的影响机制,该文采用室内模拟试验方法,在距地表40～45 cm处均匀铺设同一隔层厚度(5 cm)段状秸秆(SL)、颗粒状秸秆(SK)与粉末状秸秆(SF),以不埋设秸秆隔层为对照(CK),并利用CT扫描和图像处理技术,定量分析不同形态秸秆隔层内部孔隙度、孔隙大小分布、连通度等孔隙参数及其与灌溉水入渗特征之间的关系。结果表明:与CK处理相比,不同物理形态的玉米秸秆隔层均显著提高了40～45 cm隔层处总孔隙度,其中以SF处理最高,分别较CK、SL与SK处理显著提高了29.25、12.09与12.61个百分点(P 0.05),但SL与SK处理间差异不显著。SF处理在灌溉水入渗各阶段均显著增加了孔隙直径≤1 mm的孔隙度(P 0.05),入渗开始前、结束后分别较SL与SK处理提高了19.18、17.25和9.45、9.41个百分点,从而增大了封闭气体体积,导致灌溉水入渗速率较慢,而SL处理在灌溉水入渗开始前孔隙直径1 mm孔隙度较SK处理提高了1.04个百分点,但在入渗结束后SK处理孔隙直径1 mm孔隙度较SL处理显著提高,这种 1 mm大孔隙的变化导致SK处理出现湿润锋通过秸秆隔层耗时长于SL处理,而湿润锋通过隔层后至土柱底部耗时短于SL处理的现象。相关性分析结果表明:湿润锋通过秸秆隔层用时与入渗开始前、结束后隔层处总孔隙度、≤1 mm直径孔隙度均呈极显著正相关,与入渗前 1 mm直径孔隙度呈极显著负相关(P 0.01),而在入渗结束后这种大孔隙的作用减弱,但≤1 mm孔隙度的阻渗作用依旧显著,孔隙连通度对灌溉水入渗速率的影响较总孔隙度小。可见,不同形态玉米秸秆隔层均影响灌溉水入渗过程,CT扫描技术可作为定量研究秸秆隔层内部孔隙结构及其对灌溉水入渗影响机制的手段,该结果可为筛选和建立盐碱土壤最佳灌溉淋盐效果的物理形态秸秆隔层提供参考。     

基于轻量级CNN的植物病害识别方法及移动端应用

为了实现在手机端植物病害叶片检测,对MobileNet和Inception V3 2种轻量级卷积神经网络进行迁移学习,得到2种作物病害分类模型,将2种分类模型分别移植到Android手机端,在识别精度、运算速度和网络尺寸之间进行平衡,选择最优模型。试验表明,MobileNet和InceptionV3在PlantVillage数据集(共38类26种病害)上平均识别率分别是95.02%和95.62%。在自建图像集葡萄病害叶片的识别中MobileNet和InceptionV3平均识别率分别是87.50%、88.06%,Inception V3的整体识别精度略高,但MobileNet在所有类别的识别上均衡性更好;在模型尺寸方面Inception V3的模型尺寸大小为87.5 MB,MobileNet的模型尺寸为17.1 MB,大约是后者5倍;2种模型移植到手机端时,MobileNet和Inception V3的APP所占内存分别是21.5和125 MB;在手机端单张图片的识别时间方面,Inception V3平均计算时间约是174 ms,MobileNet的平均计算时间约是134 ms,后者的平均计算时间比前者快40 ms;在手机端MobileNet相比于Inception V3占用内存更小,运算时间更快。说明MobileNet更适合在手机端进行植物病害识别应用。