Sequence of the canine c-yes proto-oncogene

Cloning of the canine yes oncogene was attempted from a c library derived from a healthy canine spleen using a human c-yes-1 probe. The nucleotide and amino acid sequences revealed that the canine yes gene contained an open reading frame consisting of 539 amino acids. Its product had a molecular mass of 60,368 Daltons and showed 95·9 per cent and 90·4 per cent homology with human and chick p61^c-yes, respectively. Moreover, the product had a myristylation signal, src homology region (SH) 3, SH2, and tyrosine kinase domains showing 98·8 per cent and 96·0 per cent homology with those of human beings and chickens, respectively. These findings indicate that the products of the canine yes gene may have non-receptor-type tyrosine kinase activity on the cell membrane, as is the case in human and chick p61^c-yes     

西杂牛连续埋植“畜大壮”效果观察

本试验对甘肃河西地区西杂１－２岁和周岁以下的犊牛连续用促生长剂“畜大壮”分阶段进行耳根埋植,试验结果表明,４－６月龄犊牛全期１８０天,试验组增重和平均日增重分别为５．５ｋｇ和３０８．３３ｇ,试验组比对照组提高３７．２５ｋｇ和２０６．９４ｇ（Ｐ〈０．０１）,１－２岁牛全期１８０天,试验组平均增重和日增重分别为５６．４３ｋｇ和３１４．４９ｇ,试验缄比对照组分别提高３０．０３ｋｇ和１６６．８２ｇ差异显著     

香菇干制微机监控系统研究

本文研究香菇干制过程的计算机控制。在研制了系统硬件之后,根据实验结果,确定了控制对象的广义数学模型,用飞升曲线法求取模型中各参数。用计算机仿真技术对几种常用的算法进行比较,得出了采用大林算法的控制方案。     

‘Plantibodies’: a flexible approach to design resistance against pathogens

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.     

应用灰色关联度分析法综合评价高羊茅品种初探

应用灰色关联度分析法,对2000～2002年本院引进的13个高羊茅品种的适应性鉴定结果进行评估,其评估结果与品种的实际表现基本一致,说明应用关联度分析法综合评价草坪品种是可行的。分析结果:皇后、贝克、上南都的关联系数与理想品种最接近,是种植高羊茅草坪的首选品种。     

Age-related resistance to Pseudomonas syringae pv. tomato is associated with the transition to flowering in Arabidopsis and is effective against Peronospora parasitica

As plants mature it has been observed that some become more resistant to normally virulent pathogens. The ability to manifest the Age-Related Resistance (ARR) response in Arabidopsis to Pseudomonas syringae pathovars tomato (Pst) coincided with the transition to flowering in plants both delayed and accelerated in the transition to flowering. ARR was also associated with a change in PR-1 gene expression, such that young plants expressed PR-1 abundantly at 3 days post inoculation (dpi) while mature plants expressed much less. The Arabidopsis ARR response requires SA accumulation via isochorismate synthase (ICS1) [24]. ICS1 was expressed one dpi with virulent and avirulent Pst in both young and mature plants. The ARR response was also effective versus avirulent Pst providing an additional 4-fold limitation in bacterial growth. Arabidopsis ARR was found to be ineffective against two necrotrophs, Erwinia carotovora subspecies carotovora (bacterium) and Botrytis cinerea (fungus) and one obligate biotroph, Erysiphe cichoracearum (fungus). However, mature wild type, SA-deficient sid2 and NahG plants supported little growth of the obligate biotrophic oomycete, Peronospora parasitica. Therefore ARR to P. parasitica appears to be SA-independent, however the level of ARR resistance was somewhat reduced in these mutants in some experiments. Thus, there may be numerous defence pathways that contribute to adult plant resistance in Arabidopsis.     

保护地番茄新品种苏粉8号的选育

苏粉8号系以GB9736为母本,TM9761为父本配制而成的适合保护地栽培的中熟番茄一代杂种.属无限生长型.果实高圆形,粉红色,果面光滑,果皮厚,耐贮运,品质佳,可溶性固形物含量5.0%,酸甜适中,单果质量200～250 g,产量6 000kg·(667 m2)-1以上.高抗ToMV-0,1株系、叶霉病,抗枯萎病,中抗CMV.已在江苏、山东、四川、浙江等地示范推广逾266.7 hm2.     

花椰菜新品种———津品55

 ‘津品55’为秋中早熟花椰菜一代杂种, 成熟期约60 d, 内叶合抱护球, 花球较紧, 洁白,单球质量1.0 kg左右, 产量30000 kg/hm² 左右, 比对照品种‘丰花60’增产26.0% ～43.3% , 生长势强,抗病性好。     

不同施肥期沾化冬枣对15N的吸收、分配及利用特性

 盆栽条件下利用¹⁵N示踪技术, 研究不同时期施15N - 尿素, 对沾化冬枣¹⁵N的吸收利用及分配特性的影响。结果表明: 生长季前期(萌芽前和花前) 施用¹⁵N - 尿素, 经根系吸收后, ¹⁵N优先分配到贮藏器官(包括主干、多年生枝和粗根) 中, 然后外运用于树体新生器官(包括枣吊及其叶片、新生营养枝、细根及果实) 的形成, 果实采收后¹⁵N开始向贮藏器官回流; 果实硬核期¹⁵N直接用于树体营养生长和生殖生长, 而不是先贮藏再利用; 果实速长期¹⁵N优先向贮藏器官中积累; 萌芽前施¹⁵N在树体内的运转规律符合落叶果树贮藏N营养分配规律, 优先转运到生长中心。随着施肥期的后延, 植株对¹⁵N - 尿素的当季利用率逐渐下降。     

平阴玫瑰花粉亚显微形态及品种分类研究

 对平阴玫瑰24个品种的花粉亚显微形态进行了扫描电镜观察研究, 并进行了主成分分析和聚类分析。结果表明, 在花粉亚显微形态特征方面, 各品种之间既有共性, 也存在明显差别, 特别是外壁纹饰的不同可作为玫瑰品种鉴别的重要依据。聚类分析将平阴玫瑰花粉分为两大类, 即玫瑰系和蔷薇系, 与传统形态分类结论基本一致, 但也存在一定的差异。