Protective effect of vitamin C and lycopene on the in vitro fertilization capacity of sex-sorted bull sperm by inhibiting the oxidative stress

The fertilization capacity of sex-sorted sperms is seriously decreased, which inhibits its wide application. However, little information is still available about the effect of vitamin C (VC) and lycopene (Lyc) on the fertilization capacity of sex-sorted bull sperm. In this study, the washing medium and fertilization medium of sex-sorted sperm from three bull individuals were supplemented with different concentrations of VC (0, 1 × 10^–3, 1 × 10^–4, 1 × 10^–5, 1 × 10^–6 M) or Lyc (0, 1 × 10^–4, 1 × 10^–5, 1 × 10^–6, 1 × 10^–7). After washing twice and incubation for 1.5 hr, the malondialdehyde (MDA) level, phosphatidylserine (PS) translocation, membrane potential (Δψm) and IVF (in vitro fertilization) ability of sex-sorted sperm were investigated. For the sex-sorted sperm of bulls A, B and C, 1 × 10^–3 M VC or 1 × 10^–4 M Lyc treatment significantly decreased their MDA levels and PS translocation and increased their Δψm levels and cleavage rates after IVF. When blastocysts were concerned, 1 × 10^–4 M Lyc significantly improved the blastocyst rates and their IFN-tau expression of bulls A and C. In conclusion, supplementation of 1 × 10^–3 M VC or 1 × 10^–4 M Lyc in washing and fertilization medium contributed greatly to improving the fertilization capacity of sex-sorted bull sperm during IVF procedure.     

用逆转录病毒法制作鸡输卵管生物反应器的研究

本文综述了鸡的生物反应器和逆转录病毒法的研究，目前逆转录病毒法是制作鸡生物反应器的最合适的方法。     

近红外光谱分析技术在测定饲料中植酸磷含量上的应用

植酸是一种抗营养因子。传统的用三氯化铁沉淀植酸的化学分析法费时费工,不能满足饲料工业发展的需要。为了寻求快速检测饲料中植酸磷(简称P.P.)含量的手段,本试验运用近红外光谱分析技术(简称 NIRS),分别以植酸磷含量较高的米糠饼和植酸磷含量较低的高粱为样品,对这两种饲料的化学分析法测值与近红外光谱法测值进行了比较。试验结果表明:用48个米糠饼样品和50个高粱样品进行定标,相关系数(R,下同)分别为0.901和0.890,残余标准差(RSD,下同)分别为0.06和0.03;另用不参与定标的25个米糠饼样品和21个高粱样品对定标结果进行检验,R 分别为0.803和0.917,RSD 分别为0.07和0.02。NIRS 法的分析精度基本上可以达到化学分析法的要求。作者认为,达项技术作为饲料中 PP 含量快速分析的手段是可行的。     

Association of a missense mutation in the luteinizing hormone/choriogonadotropin receptor gene （LHCGR） with superovulation traits in Chinese Holstein heifers

Background: Upon binding luteinizing hormone in the ovary, the luteinizing hormone/choriogonadotropin receptor (LHCGR) is necessary for follicular maturation and ovulation, as well as luteal function. We detected mutations in the LHCGR gene and evaluated their association with superovulation. Methods: Using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing, we examined polymorphisms in LHCGR and the genotypes associated with superovulation traits in 127 Chinese Holstein heifers. Results: A G/T polymorphism (ss52050737) in exon 11 was significantly associated with the total number of ova and the number of transferable embryos. Conclusions: LHCGR may be a new predictor for superovulation in Chinese Holstein heifers.     

养鸡八禁忌

一、禁与鸭鹅混养：有的农户为节省饲养场地，把鸡、鸭、鹅关在一起混养，这是不科学的。一是混养易造成疫病的互相传播，如鸭、鹅也能感染新城疫病毒，在一般情况下，病毒潜伏于体内，成为带毒，不表现任何症状。若与鸡混养在一起，极易将病毒传染给鸡，导致鸡发生新城疫。二是鸡鸭鹅混养在一起，会使饲料利用率降低，     

巴比妥类镇静剂的检测研究进展

本文对巴比妥类安眠镇静剂检测方法的研究进展进行了综述。重点讨论了样品前处理方法及各种衍生化方法,GC、GC-MS、HPLC、HPLC-MS等检测方法。     

三江白猪组织基因组提取及RAPD反应条件的优化

本试验从三江白猪组织中提取总DNA,获得较高质量的模板样品。并对RAPD反应的条件进行了优化,提高RAPD分析的可靠性和重复性。确保三江白猪遗传分析的真实性和准确性,为进一步的研究提供参考条件。     

病羊临床诊断中的消化系统检查

对羊只的系统检查是患病羊临床诊断的重要组成部分,消化系统检查是临床检查中最常见的检查。临床实践中,要特别注意对病羊的采食饮水、反刍嗳气以及各消化器官进行认真细致、有针对性的检查。