江西省新型农业社会化服务体系的特点与运行模式

实地调研江西省新型农业社会化服务体系建设现状，对江西省目前的新型农业社会化服务体系的特点与运行模式进行分析，针对当地在服务体系构建过程中出现的问题，提出新时期的发展建议。     

云南省昆明市执业兽医考试现状及管理措施

昆明市通过加强考务工作,积极宣传动员,建立长效机制等举措,近年来执业兽医考试工作成效显著,2010—2018年昆明市执业兽医累计报考4 819人,其中(助理)执业兽医师共考取通过1 222人。通过对2010—2018年的考试情况分析发现:昆明市报考人数逐年上升,学历水平不断提升;报考人员职业以动物诊疗机构、学生、非兽医从业人员为主;报考人员通过率以科研教育人员、诊疗机构、学生等居前列。上述情况提示,今后应进一步优化执业兽医注册备案管理,强化行业对执业兽医的重视与认可,完善执业兽医考试和继续教育制度。     

优质黑猪育种30年工作体会

品种是影响养猪生产中的最重要因素,猪育种是一项复杂且系统的工程,文章从育种素材筛选、育种方案设计、育种方法选择、品种审定以及扩群产业化推广等方面进行详细的阐述,并结合育种经验提出相应猪育种建议。     

基于叶片元素的配方施肥对草莓产量和品质的影响

为了探讨以草莓叶片元素为参考得出的施肥配方对草莓果实产量和可溶性固形物含量(SSC)的影响,以草莓品种"圣诞红"为试材,先利用叶片元素分析法测定草莓矿质元素的含量,确定了3种施肥配方(配方1、2、3),进而利用比例注肥泵的水肥一体化系统对温室中栽培的"圣诞红"进行施肥和管理。通过定期检测果实SSC和统计产量来评价配方效果。结果表明:利用配方1、2、3施肥管理的草莓果实在总产量、平均单株产量、平均单株果数和单果质量上都显著高于常规施肥方式下草莓果实的各参数(P0.05);配方3施肥下,草莓在果实产量、平均单株产量和单果质量上高于配方1和配方2施肥下的果实;配方2施肥下的草莓SSC最高,但与配方1和配方3施肥下的草莓SSC无显著差异。说明草莓种植时更适合满足其自身需求的营养元素供给,以草莓叶片矿质元素分析为依据的施肥配方明显提高了其果实产量和SSC,多组比例注肥泵并联使用保证了其配方肥料的均衡比例和投加的精准度,该方法更具科学和合理性,具有应用推广前景。     

转角法施工镀锌高强螺栓关键技术

常州现代传媒中心主塔楼屋顶高88 m的钢塔(塔桅钢结构)设计工艺复杂,施工难度极大,所有钢构件均进行热浸锌防腐处理,采用8.8级热浸锌高强螺栓连接。考虑到钢结构拼接节点热浸锌高强螺栓的安装质量直接影响塔桅钢结构的安全和稳定,通过对热浸锌高强螺栓施工工艺进行反复论证、试验,最终确定采用转角法施工热浸锌高强螺栓,取得了理想的施工效果,确保了工程施工质量。     

Evolution Analysis About Soybean MIR166 Family

MicroRNA(miRNA) is a class of important regulating non-coding small molecular RNA. The gma-MIR166 gene family consists of 21 members and their expression patterns diversify widely. It is important to analyze the evolution of gma-MIR166 gene family in order to understand the evolutionary mechanisms of miRNAs in soybean. In this study, we implemented soybean wide genome block analysis, the molecular phylogeny of gma-MIR166 and block analysis of gma-MIR166 family. The results showed that both chromosome big segmental duplications and tandem duplications were main reasons contributed to the expanding of gmaMIR166 gene family. These findings suggested that gma-MIR166 gene family might originate from one or two ancient miRNA genes. The results of research provided a support for evolutionary study of miRNAs in soybean and related species in Fabaceae.     

Effects of phytosterol ester on aortic aging and expression of related genes in rats

AIM: To explore the protective effect of phytosterol ester (PSE) on aortic aging in rats. METHODS: The female SD rats (12 months old, n=42) were randomly divided into control group, model group and PSE group. During the experiment, the rats in control group, model group and PSE group were treated with basic feed, high-fat diet (HFD) and HFD with 2% PSE (W/W) for 6 months, respectively. The morphological changes of the aorta were observed by HE staining and Masson staining, and the absolute area of smooth muscle cells and collagen fiber in the vascular wall were measured by image analysis. The levels of advanced glycosylation end products (AGEs), malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) in the plasma were detected. The expression of silent information regulator 1 (SIRT1) and peroxisome proliferator-activated receptor γ (PPARγ) at mRNA and protein levels in the vascular tissue was determined by real time PCR and Western blot, respectively. RESULTS: PSE significantly lowered plasma TC and LDL-C, and increased plasma HDL-C level (P<0.05), but had no effect on plasma TG level. PSE significantly attenuated the thickening of intima and media of aging aortic, and decreased the migration of vascular smooth muscle cells (VSMC) and the amount of VSMC and collagen fiber in the aorta (P<0.05). PSE significantly reduced the contents of AGEs and MDA (P<0.05), but had no effect on the activity of SOD and CAT in the plasma. PSE also down-regulated the expression of PPARγ and up-regulated the expression of SIRT1 (P<0.05). CONCLUSION: PSE is able to attenuate the senescence process in the aorta by reducing the production of reactive oxygen species in plasma, and activating SIRT1, or inhibiting the expression of PPARγ in vascular tissues.