多角体蛋白基因核苷酸序列对HBsAg(PreS2+S)在家蚕中表达的作用

应用PCR突变的方法 ,在乙肝病毒表面抗原 (HBsAg)前S2序列的 5’端融合了BmNPV多角体蛋白基因5’端的 12个碱基 ,获得了融合乙肝表面抗原中蛋白基因 (HBMp) ;通过同源重组将其插入到BmNPV基因组多角体启动子后 ,构建了重组杆状病毒BmPAK HBMp。用重组病毒BmPAK HBMp和BmPAK HBM(带有非融合乙肝表面抗原中蛋白基因 )感染家蚕细胞及蛹 ,对两种病毒的表达产物用ELISA进行了跟踪检测 ,结果表明融合蛋白比非融合蛋白HBsAg活性提高了 6 0 %～ 80 % ,作为HBsAg构成部分的PreS2抗原性提高了 8～ 10倍 ;并且HBsAg和PreS2 Ag表达的时相曲线不同 ,PreS2 Ag的表达量比HBsAg早 1～ 2d达到最大值。ELISA检测和Westernblotting分析表明 ,多角体基因序列的存在更有利于中蛋白全基因 (PreS2 +S)的表达。     

证券技术分析何以预测

证券技术分析自诞生以来，其合理性和有效性就一直受到了普遍的关注。有些人认为技术分析的作用非常有限，甚至是毫无根据和无效的。这一种观念在有效市场假说创立以来更上升到了理论的高度。本文认为，技术分析作为一种在证券投资中被广泛应用的预测股价变动的工具，具有一定的内在合理性因素，可以用来对股价变动的趋势作出预言。但必须指出:技术分析的科学性尚不能得到承认。     

农牧交错区不同牧草时期放牧奶牛采食量与食性的研究

用饱和烷烃法对不同牧草时期放牧奶牛的采食量进行了测定.结果表明:放牧奶牛在牧草幼嫩期、旺盛期、枯黄期3期的采食量结果分别为:绝对采食量(kg/d)为11.54±0.66、13.83±0.55、5.75±0.32,采食量在牧草旺盛期时达到最高.采食组成幼嫩期以苔草为主、旺盛期以隐子草为主、枯黄期以赖草为主,这与草场植物的生长和奶牛的喜好有关.     

Molecular cloning and functional analysis of goose interferon gamma

The cDNA for goose interferon gamma (goIFN-gamma) was cloned from PHA-stimulated goose peripheral blood mononuclear cells (PBMCs) by RT-PCR. This cDNA encodes a 19-amino acid signal peptide and a 145-amino acid mature protein, which shares a high homology with duck IFN-gamma. Recombinant mature goose interferon gamma (rgoIFN-gamma) generated from both prokaryotic and eukaryotic expression systems effectively inhibited the replication of goose paramyxovirus and recombinant vesicular stomatitis virus in vitro. These antiviral activities were abrogated by rabbit anti-rgoIFN-gamma antibodies in vitro. Furthermore, rgoIFN-gamma stimulated goose peritoneal macrophages to produce nitric oxide (NO) in vitro, demonstrating its macrophage activating factor (MAF) activity. Therefore, the availability of bioactive rgoIFN-gamma and its specific antibodies provides valuable tools for studying T cell immunity in geese.     

Molecular cloning, in vitro expression and bioactivity of goose B-cell activating factor

B-cell activating factor (BAFF), belonging to the TNF family, is critical for B cell survival and maturation. cDNA of goose BAFF (gBAFF) was amplified from goose spleen by RT-PCR. The open reading frame (ORF) of gBAFF encodes a protein of 288-amino acid. The gBAFF shows 98, 92, 44 and 55% amino acid sequence identity with duck (dBAFF), chicken (cBAFF), mouse (mBAFF) and human BAFF (hBAFF), respectively. RT-PCR results showed that gBAFF mRNA is expressed in thymus and more highly expressed in the bursa of Fabricius and spleen. Recombinant soluble gBAFF (gsBAFF) expressed in Escherichia coli has molecular weight of approximately 19kDa. In vitro, purified gsBAFF was able to promote bursa B cells survival/proliferation in goose, duck and chicken. Furthermore, recombinant dsBAFF and csBAFF have a positive effect on goose, duck and chicken bursa B cells survival/proliferation. These findings indicate that gBAFF plays an important role in the survival/proliferation of goose B cells and, owing to its high evolutionary conservation, functional cross-reactivity exists between chicken, duck and goose BAFF.     

Role of calreticulin-induced mitochondrial damage in high glucose-induced apoptosis of myocardial cells

AIM: To observe the effect of high glucose on the protein expression of calreticulin (CRT) and its association with cell apoptosis and mitochondrial dysfunction in the cardiomyocytes. METHODS: AC-16 cardiomyocytes were randomly divided into normal glucose group, high glucose group, high glucose+ CRT siRNA group and isotonic control group. The cell apoptotic rate, reactive oxygen species (ROS), mitochondrial membrane potential level, respiratory enzyme activity, and protein expression of CRT were observed. RESULTS: Compared with the cardiomyocytes in normal glucose group, the apoptotic rate and ROS production of cardiomyocytes increased in high glucose group, accompanying with the decreases in the mitochondrial membrane potential level and enzyme activitiy of the respiratory chain. The protein expression of CRT was significantly increased in high glucose group. However, compared with high glucose group, high glucose+ CRT siRNA decreased the expression of CRT and attenuated the damage of mitochondria, but CRT siRNA did not reduce the ROS level in cardiomyocytes. CONCLUSION: High glucose brings about CRT over-expression to induce mitochondrial injury, thus increasing myocardial apoptosis.     

盐胁迫下沙柳幼苗的生理生化变化

以沙柳幼苗为试材,研究了0(对照)、50、100、150mM NaCl胁迫对沙柳幼苗的形态变化、酶液宏观变化、酶活性变化及内源激素含量变化的影响。结果表明:100mM NaCl盐浓度及以下胁迫对沙柳幼苗近乎无影响,ABA、IAA、GA的含量相对平稳,无太大变化,通过测定内源激素的含量可发现,100mM NaCl盐浓度时沙柳幼苗长势出现下降,100mM NaCl盐浓度以上的胁迫对沙柳幼苗的影响显著,沙柳幼苗所能承受NaCl迫害的最高Na盐浓度处于100mM NaCl和150mM NaCl之间。150mM NaCl盐浓度胁迫时,其在第1天和第3天间代谢紊乱程度达到其可耐受的极限程度,随后的几天植株走向死亡。同时沙柳幼苗在濒临死亡时其ABA含量无大幅度升高,这与常见植物有所不同。而IAA、GA的含量呈现出在短时间内迅速达到最大含量,随后的几天内含量有所降低。在极高浓度的NaCl胁迫下,沙柳幼苗的SOD活性在短时间内迅速下降后持续大幅度上升,沙柳幼苗死亡时,SOD活性达到最高。沙柳幼苗受到NaCl胁迫时,其CAT比SOD和POD敏感,CAT活性受到的影响更显著。     

PEG处理对提高一串红种子活力的研究

本文以优，中，劣三个活力不等的一串红种子为材料，研究了PEG不同浓度和不同处理时间对种子发芽率的影响，结果表明，（1）PEG可明显改善中，低活力种子细胞膜透性，显著提高种子发芽率；（2）劣质一串红种子，用25%PEG处理1天或10%处理4天，其发芽率比对照提高16.12倍，19.2倍，中等活力一串红种子用10%PEG处理1天较佳；而优良种子经过处理，反而效果不好。     

木(木尞)种子小蜂生活习性及防治研究

在木(木尞)果实子叶开始发育以前,不论先后孵化的幼虫,均不造成为害,幼虫发育极慢,一直处于低龄阶段.子叶一旦发育,幼虫便钻入胚内迅速发育进行为害.成虫产卵末期到木(木尞)果子叶发育前(约20—30天)这段时间是化防的关键时期.化防采用超低量或常量喷撒久效磷,防治效果一般在70.88—88%,个别的达97.8%,无药害.     

我国杂交水稻种业发展浅谈

文章简要分析了"入世"后我国杂交水稻种业面临的有利因素和不利因素,对发展我国杂交水稻种业提出了具体对策,即进一步完善种子法规,加强种子市场的管理,改革土地制度,改革国有种业体制,扶持民营种业发展,创建具有足够竞争力的中国种业集团,充分开展国际合作,确保科技研发优势。