全文获取类型
收费全文 | 34609篇 |
免费 | 958篇 |
国内免费 | 1907篇 |
专业分类
林业 | 6102篇 |
农学 | 4119篇 |
基础科学 | 1735篇 |
5760篇 | |
综合类 | 5927篇 |
农作物 | 2967篇 |
水产渔业 | 2507篇 |
畜牧兽医 | 3490篇 |
园艺 | 1638篇 |
植物保护 | 3229篇 |
出版年
2024年 | 49篇 |
2023年 | 207篇 |
2022年 | 487篇 |
2021年 | 690篇 |
2020年 | 661篇 |
2019年 | 587篇 |
2018年 | 3116篇 |
2017年 | 3257篇 |
2016年 | 1749篇 |
2015年 | 840篇 |
2014年 | 758篇 |
2013年 | 912篇 |
2012年 | 1797篇 |
2011年 | 3203篇 |
2010年 | 3131篇 |
2009年 | 2299篇 |
2008年 | 2308篇 |
2007年 | 2599篇 |
2006年 | 1073篇 |
2005年 | 1091篇 |
2004年 | 543篇 |
2003年 | 549篇 |
2002年 | 361篇 |
2001年 | 374篇 |
2000年 | 468篇 |
1999年 | 569篇 |
1998年 | 510篇 |
1997年 | 462篇 |
1996年 | 457篇 |
1995年 | 388篇 |
1994年 | 342篇 |
1993年 | 318篇 |
1992年 | 298篇 |
1991年 | 266篇 |
1990年 | 193篇 |
1989年 | 171篇 |
1988年 | 138篇 |
1987年 | 78篇 |
1986年 | 45篇 |
1985年 | 29篇 |
1984年 | 19篇 |
1983年 | 17篇 |
1982年 | 15篇 |
1981年 | 23篇 |
1980年 | 10篇 |
1979年 | 4篇 |
1977年 | 4篇 |
1968年 | 4篇 |
1965年 | 1篇 |
1964年 | 1篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
211.
212.
鸡源和鸭源EDS病毒的某些生物学特性比较 总被引:2,自引:0,他引:2
对 E D S 病毒鸡源株 ( N E4) 和鸭源株 ( J E1) 在繁殖动态、毒价、免疫原性、核酸酶切图谱、多肽组成等5 个方面进行比较研究。结果表明, 2 株相同血清型、不同宿主来源的 E D S 病毒在普通生物学特性方面基本相同, 但在核酸酶切图谱和多肽组成等分子生物学水平上存在明显差异。 相似文献
213.
214.
Detection of antibodies in serum and egg yolk following infection of chickens with an H6N2 avian influenza virus. 总被引:3,自引:0,他引:3
Darrell W Trampel En-Min Zhou Kyoung-Jin Yoon Kenneth J Koehler 《Journal of veterinary diagnostic investigation》2006,18(5):437-442
Active serologic surveillance programs to detect avian influenza viruses (AIVs) in table egg-laying chickens have been initiated by several states as a response to the economic threat posed by these viruses. Most outbreaks of avian influenza in domestic poultry are caused by mildly pathogenic AIVs. In the study reported here, infection by an H6N2 AIV was used as a model of mildly pathogenic AIV infections in egg-type chickens. The total number of eggs laid by 5 control hens was 619 or 0.904 eggs/day/hen, whereas the total number laid by 10 infected hens was 1,018 or 0.743 eggs/day/hen. The difference in egg production between the 2 groups was not statistically significant (P = 0.38). Anti-influenza antibodies were monitored by use of an agar gel immunodiffusion test and an ELISA for a period of 20 weeks after inoculation. Antibodies in serum developed sooner, peaked at higher levels, and remained at higher levels than did antibodies found in egg yolk, as indicated by ELISA results. For infected chickens, the correlation between serum and egg yolk ratios was 0.66. Serum samples would appear to be preferable to egg yolk samples for surveillance programs intended to identify chicken flocks that may have been infected by an AIV weeks or months before samples are collected. 相似文献
215.
216.
Zirui Zhou Jie Zhang Xianlian Zhang Shilan Mo Xian Tan Lixia Wang Jianzhong Li Yali Li Xueqin Ding Xianyong Liu Xingqun Ma Huansheng Yang Yulong Yin 《Journal of animal physiology and animal nutrition》2019,103(5):1530-1537
Weaning process widely affects the small intestinal structure and function in piglets, while the responses of large intestine to weaning stress are still obscure. The purpose of this study was to determine the developmental changes (i.e., short chain fatty acids (SCFAs) concentrations, growth parameters, crypt‐related indices and antioxidant capacity) in colon of piglet during weaning. Forty piglets were weaned at day 21 and euthanized to collect colonic tissues and digesta samples on day 0, 1, 3, 7 and 14 post‐weaning (n = 8). Piglet growth performance was improved (p < .001) on day 7 and 14 post‐weaning. The concentrations of acetate, propionate, butyrate, valerate, isobutyrate, isovalerate and total SCFAs were higher (p < .001) during the late post‐weaning period. The mRNA abundances of SCFAs transporters were greater (p < .001) on day 7 and 14. The absolute and relative weights, absolute length and perimeter of colon were greater (p < .001) on day 7 and 14. Similarly, post‐weaning increases (p < .001) in colonic crypt depth and Ki67 positive cells numbers per crypt were observed during the same period. Colonic crypt fission indices decreased (p < .01), while total crypt numbers increased (p < .001) on day 14 after weaning. Moreover, total SCFAs concentration was significantly associated with colonic growth parameters and Ki67 cells/crypt (p < .001). In addition, catalase content was decreased on day 3, 7, and 14, whereas, the concentrations of total superoxide dismutase (T‐SOD) and manganese‐containing superoxide dismutase (MnSOD) were higher (p < .05) on day 1 and 3 post‐weaning. These results showed that weaning process has a significant effect on colonic growth and development, which might be associated with the change of SCFAs concentrations in colon. 相似文献
217.
The objective of this study was to analyse the effects of different levels of rumen-degradable protein (RDP) on intake, digestibility
and microbial protein synthesis by supplementing mustard oil cake (MOC) on rice straw-based diet of cattle (Bos indicus) in Bangladesh. A 4 × 4 Latin square design was applied. Four diets having constant energy (7.0 MJ/kg of dry matter (DM))
with varying levels of RDP (M
0 = 4.1 g/MJ (control), M
1 = 6.3 g/MJ, M
2 = 8.3 g/MJ and M
3 = 12.4 g/MJ of metabolizable energy (ME)) were received by each animal for a period of 28 days. A metabolism trial was conducted
for 7 days. Results indicate that with increasing levels of RDP, crude protein (CP) and RDP intake increased significantly
(P < 0.01). The significant (P < 0.01) increase in digestibility values are obtained for DM, organic matter, CP and digestible organic matter in the rumen.
The digestibility of neutral detergent fibre and acid detergent fibre was also increased significantly (P < 0.05). The total nitrogen (N), ammonia-N and total volatile fatty acids increase significantly (P < 0.01) while the rumen pH increased from M
0 to M
2 and decreased thereafter. The efficiency microbial N intake increased significantly (P < 0.01) but showed a curvilinear response with higher RDP level (12.40 g/RDP/MJ ME). This study concludes that supplementation
of RDP from MOC enhances the intake, digestibility and microbial protein synthesis which ultimately increases utilization
of low-quality feed resources that can be used for developing cost-effective feeding systems on a straw-based diet in tropical
regions. 相似文献
218.
Indigenous chicken (IC) and their production systems were characterized to understand how the whole system operates for purposes
of identifying threats and opportunities for holistic improvement. A survey involving 594 households was conducted in six
counties with the highest population of IC in Kenya using structured questionnaires. Data on IC farmers’ management practices
were collected and analysed and inbreeding levels calculated based on the effective population size. Indigenous chicken were
ranked highest as a source of livestock income by households in medium- to high-potential agricultural areas, but trailed
goats in arid and semi-arid areas. The production system practised was mainly low-input and small-scale free range, with mean
flock size of 22.40 chickens per household. The mean effective population size was 16.02, translating to high levels of inbreeding
(3.12%). Provision for food and cash income were the main reasons for raising IC, whilst high mortality due to diseases, poor
nutrition, housing and marketing channels were the major constraints faced by farmers. Management strategies targeting improved
healthcare, nutrition and housing require urgent mitigation measures, whilst rural access road network needs to be developed
for ease of market accessibility. Sustainable genetic improvement programmes that account for farmers’ multiple objectives,
market requirements and the production circumstances should be developed for a full realization of IC productivity. 相似文献
219.
A study was undertaken from October 2006 to March 2007 to determine the prevalence and antimicrobial resistance patterns of Salmonella serovars. Liver, mesenteric lymph nodes, intestinal content, and carcass swab samples (each n?=?186) were collected from 186 apparently healthy slaughtered cattle at Bahir Dar abattoir. Bacteriological analysis was done according to the International Organization for Standardization (ISO 6579 2002). Isolates were serotyped at Agence Française de Securite Sanitaire des Aliments, Cedex, France. Twenty-eight isolates consisting of Salmonella Typhimurium, Salmonella Newport, Salmonella Haifa, Salmonella Heidelberg, Salmonella Infantis, and Salmonella Mishmarhaemek were identified. Salmonella Typhimurium and Salmonella Newport were most frequently isolated while Salmonella Heidelberg and Salmonella Mishmarhaemek were isolated least. Eleven of the 28 (39.3%) were resistant to one or more of the antimicrobials tested. Resistance was shown to ampicillin, chloramphenicol, gentamycin, norfloxacin, polymyxin-B, streptomycin, tetracycline, and trimethoprim. Four of 11 (36.4%) were multiple antimicrobial resistant. All the isolates tested were susceptible to the antimicrobial effects of gentamycin, norfloxacin, and trimethoprim. Eleven, four, and two isolates of the 28 were resistant to streptomycin, tetracycline, and ampicillin, respectively. All isolates of Salmonella Infantis, Salmonella Typhimurium (except one), and Salmonella Mishmarhaemek were susceptible to the tested antimicrobials. One Typhimurium isolate was resistant to chloramphenicol, streptomycin, and tetracycline. Salmonella Haifa was multiply antimicrobial resistant to ampicillin, tetracycline, and streptomycin. All isolates of Salmonella Heidelberg were resistant to streptomycin. Results of this study indicated high level of carcass contamination with antimicrobial-resistant Salmonella serovars which could pose public health risk; suggests need for hygienic slaughtering operations and proper cooking of meat before consumption. Further detailed studies involving different abattoirs, animal products, food items, and animals on different settings were recommended in the study area. 相似文献
220.
Mahr-un-Nisa Shahzad MA Phillips CJ Sarwar M 《Tropical animal health and production》2012,44(5):947-952
Urea is a common ingredient of the diets of intensively fed lambs, but is increasingly required for industrial processes.
Maize steep liquor (MSL) is a by-product of maize grain degradation to produce starch that may be a suitable replacement.
Fifty growing lambs were fed on equinitrogenous diets in which between 0% and 80% of the urea was replaced by MSL; their growth
and metabolism were recorded over 70 days. Increasing replacement of urea by MSL increased feed intake and nutrient digestibilities,
leading to increased growth rates, more efficient feed conversion, and increased nitrogen retention. Concentrations of triiodothyroxin,
thyroxin, glucose, and methionine were increased by replacement of urea by liquor, and plasma urea was reduced. This study
suggests that MSL is a suitable replacement for up to 80% of urea in the diet of rapidly growing lambs. 相似文献