饲料中抗营养因子--单宁的含量分析方法

针对近年来单宁在动物营养上的最新研究成果,本文重点阐述了饲料中缩合单宁、可水解单宁和总酚类化合物的分析方法。旨在为更好地利用含单宁饲料提供参考。     

水禽禽流感防控的探讨

水禽是流感病毒巨大的贮存库,其在禽流感的发生及其传播中具有非常重要的流行病学意义。本文就水禽禽流感的流行特点、致病力及其防控措施中疫苗的选择、免疫程序的制定、免疫增强剂等方面作了阐述和探讨。     

应用Excel软件分析敌敌畏农药对两广二号家蚕的安全间隔期

应用Excel软件中的曲线方程分析判断敌敌畏农药对两广二号家蚕的安全间隔期。结果表明：敌敌畏1000倍液的安全间隔期是6d多,其数学模型为幂函数曲线方：y=88．0653x-1834本方法克服以往家蚕的农药安全期无数学模型可循或使用的BASIC方法陈旧繁杂的缺点,也避免英文统计软件SPSS难以在农村普及的不足,是一种计...     

Clinicopathology of gout in growing layers induced by high calcium and high protein diets

1. An experiment was conducted to test the independent and combined effects of high dietary calcium and protein concentrations on the induction of visceral gout in growing birds of a layer strain. 2. One hundred and sixty healthy birds were randomly divided into 4 groups at 35 d of age. The different groups were given 4 diets containing normal or high concentrations of dietary calcium or crude protein in a 2 x 2 factorial experiment for 30 d. The diets contained normal calcium (Ca) and crude protein (CP) (NCNP, 8.5 g Ca/kg and 175g CP/kg), high calcium and normal protein (HC, 36.3 g Ca/kg and 175 g CP/kg), normal calcium and high protein (HP, 8.8 g Ca/kg and 245 g CP/kg) or high calcium and high protein (HCHP, 36.8 g Ca/kg and 242 g CP/kg), respectively. 3. Typical visceral gout was induced by the HCHP diet. The HCHP and HC diet caused severe kidney damage. The HP diet did not cause kidney damage, but significantly increased plasma uric acid and inorganic phosphorus concentrations. 4. The HC diet significantly increased plasma uric acid, calcium and sodium, but significantly decreased plasma inorganic phosphorus, potassium and magnesium concentrations. The HCHP diet significantly increased plasma uric acid, calcium and sodium. 5. Urine volumes were significantly higher on the HCHP and HC diets than on the control. The growers raised on HC and HCHP diets had significantly higher total quantity of 24 h urinary excretion of uric acid, calcium, magnesium, inorganic phosphorus and potassium and a significantly lower 24 h urinary excretion of sodium. The growers fed on the HP diet had a higher 24 h urinary excretion of uric acid and inorganic phosphorus than the control. 6. It is concluded that growing layer birds should not be fed on layer rations.     

我国兽用生物制品研制、生产、流通领域中的生物安全问题和应该采取的措施

生物安全是当前人们普遍关心的问题,它的影响涉及国家安全、人类社会及生态环境等诸多方面,其中由病原微生物引起的生物安全问题直接关系到动物疾病和人类健康,其重要性尤为突出,当前一些传染病的流行与我们的生物安全防范意识薄弱,措施不当有着直接的关系,必须引起我们的高度重视。我国兽用生物制品生物安全的管理问题主要表现在以下两个方面:一是如何进一步加强对兽用疫苗的质量管理和流通领域的监控,二是严格规范和控制从事动物病原微生物操作的实验室生物安全条件。对前者,我们有相应的法律法规和管理办法,关键的问题是如何进一步落实的…     

三个新西兰牧草品种在平坝县夏播种植的适应性初报

平坝县位于贵州省中部,海拔1200—1600m,年平均气温14．7℃,年降雨量1298mm,全年平均雨日194天,日照时数1296小时,无霜期273天,属北亚热带季风湿润气候,冬无严寒,夏无酷暑,是贵州省重点农业县之一。由于我县具有比较优越的气候环境和地理条件,2008年我局草地站在新西兰专家和省农业厅外经处的指导下,在我县十字乡青山村选取一块60m^2的菜地作为试验样地,对引进的3种新牧草品种——ubon雀稗、杂交象草、拉巴豆进行试验种植。     

A pig–human comparative RH map comprising 20 genes on pig chromosome 13q41 that harbours the ETEC F4ac receptor locus

The enterotoxigenic Escherichia coli (ETEC) F4ac is a major cause of diarrhoea in newborn and young pigs. The locus for the intestinal ETEC F4ac receptor (F4acR) has been mapped to pig chromosome (SSC) 13q41 with known homology to human chromosome (HSA) 3q21 and q29. However, the causative gene and mutation(s) remain unknown. The aim of this study was to characterize gene-derived markers on SSC13q41 for fine mapping of the F4acR locus, and construct a high-resolution pig–human comparative map to select positional candidate genes for F4acR. Pig-specific sequence-tagged site markers were developed for 20 genes that are located in a 6.8-Mb region on HSA3q21 and q29, and a total of 34 single-nucleotide polymorphisms (SNPs) were identified in 14 of 20 markers developed. Eighteen markers were mapped to SSC13q41, while the other two markers ( PLXNA1 and KLF15 ) were assigned to SSC13q32 and SSC7q13, respectively, by radiation hybrid mapping. This result showed that there was a small conserved segment on SSC7 corresponding to HSA3q21. A framework map comprising 18 markers on SSC13q41 was established, refining the synteny breakpoint on SSC13q41 to a region of 12.3 centiRay. The comparative radiation hybrid (RH) map revealed three interesting candidate genes for F4acR from the human genome, viz. MUC4 , MUC13 and MUC20 . Linkage analysis with six marker polymorphisms revealed that MUC4 had the most significant linkage with the F4acR locus.     

低聚果糖对小鼠肠道菌群影响与机体免疫调节关系的研究

为了研究低聚果糖对小鼠肠道菌群影响与机体免疫调节的关系,分别选取18～22 g BALB/C健康清洁级雄性小鼠与清洁级昆明种雌性小鼠进行肠道菌群和免疫指标的检测.受试对象分别以0g/(kg·Bw)、0.69g/(kg·BW)、1.38g/(kg·BW)和4.15 g/k(kg·BW)灌胃低聚果糖,于给药后20 d测定粪便中双歧杆菌、乳杆菌、肠球菌、肠杆菌、产气英膜梭菌的菌数,33 d后测定细胞免疫功能、体液免疫功能、单核-巨噬细胞功能、NK细胞活性等指标.试验结果表明,1.38 g/(kg·BW)低聚果糖能够促进乳杆菌的增加并抑制肠杆菌的增幅,4.15 g/(kg·BW)的低聚果糖能够促进乳杆菌和双歧杆菌的增加并抑制肠杆菌和肠球菌的增幅;低聚果糖可以提高小鼠的免疫力,两者之间具有关联作用.     

草食畜牧业在桂西北喀斯特环境移民地区的作用--以环江毛南族自治县为例

本文以环江毛南族自治县为例介绍了桂西北喀斯特山区生态与经济状况,阐明在喀斯特移民地区发展草食畜牧业对改善生态环境与解决贫穷问题的重要性,分析了当前发展草食畜牧业存在的问题,并提出解决方案。     

2000～2004年间广西新城疫病毒强毒株的分离与鉴定

近年来从广西不同地区规模化鸡场的13个肉鸡群病死鸡中分离到13株有血凝性的病毒,这13株病毒的血凝性均能被新城疫标准阳性血清抑制,经过血凝(HA)试验、血凝抑制(HI)试验和RT-PCR检测结果确定为新城疫病毒.13株病毒的生物学特性测定实验结果证明该13株病毒均为新城疫病毒强毒株.