A cluster of two psittacosis cases among women farmers exposed to Chlamydia psittaci-infected domestic poultry in Zhejiang Province,China

A cluster of Chlamydia psittaci (C. psittaci) cases was reported in Zhejiang Province, China, 2019. This study evaluates the extent of the outbreak and determines the source of infection. Real-time PCR and sequencing of the ompA gene of C. psittaci were performed to identify the cases, the domesticated poultry and close contacts. The index patient was a 76-year-old woman with chronic vertigo, and Case 2 was a 64-year-old female farmer with herpes zoster. Both women bought psittaci-infected chickens or ducks from the same mobile street vendor and raised them for 10 days and 23 days before fever onset. There were no direct contact between the two women. C. psittaci test was positive for the two patients, one sick chicken, three healthy ducks and the vendor's chicken cage. Phylogenetic analysis showed that all seven C. psittaci positive samples carried identical ompA genotype A of C. psittaci. Of all of the patients' 148 close contacts, none tested positive for C. psittaci, or developed acute respiratory symptoms. Both patients were discharged after a 4-week hospital stay. In conclusion, the source of this cluster was the poultry infected with C. psittaci, which occasionally cause infections in farmers, but inter-human transmission seems unlikely.     

Pharmacokinetics of sanguinarine,chelerythrine, and their metabolites in broiler chickens following oral and intravenous administration

Sanguinarine (SA) and chelerythrine (CHE) are the main active components of the phytogenic livestock feed additive, Sangrovit®. However, little information is available on the pharmacokinetics of Sangrovit® in poultry. The goal of this work was to study the pharmacokinetics of SA, CHE, and their metabolites, dihydrosanguinarine (DHSA) and dihydrochelerythrine (DHCHE), in 10 healthy female broiler chickens following oral (p.o.) administration of Sangrovit® and intravenous (i.v.) administration of a mixture of SA and CHE. The plasma samples were processed using two different simple protein precipitation methods because the parent drugs and metabolites are stable under different pH conditions. The absorption and metabolism of SA following p.o. administration were fast, with half‐life (t_1/2) values of 1.05 ± 0.18 hr and 0.83 ± 0.10 hr for SA and DHSA, respectively. The maximum concentration (C_max) of DHSA (2.49 ± 1.4 μg/L) was higher that of SA (1.89 ± 0.8 μg/L). The area under the concentration vs. time curve (AUC) values for SA and DHSA were 9.92 ± 5.4 and 6.08 ± 3.49 ng/ml hr, respectively. Following i.v. administration, the clearance (CL) of SA was 6.79 ± 0.63 (L·h^?1·kg^?1) with a t_1/2 of 0.34 ± 0.13 hr. The AUC values for DHSA and DHCHE were 7.48 ± 1.05 and 0.52 ± 0.09 (ng/ml hr), respectively. These data suggested that Sangrovit® had low absorption and bioavailability in broiler chickens. The work reported here provides useful information on the pharmacokinetic behavior of Sangrovit® after p.o. and i.v. administration in broiler chickens, which is important for the evaluation of its use in poultry.     

桑椹红色素稳定性的研究

研究了光和热等物理因素、常用食品添加剂、金属离子、氧化剂及还原剂对桑椹红色素稳定性的影响。结果表明 :pH值对桑椹色素的影响显著 ,在酸性条件下色素稳定 ;长时间高温和光照对色素有明显的破坏作用 ;金属离子Na+ 、Ca2 + 、Cu2 + 、Fe2 + 、Mg2 + 、Zn2 + 和Mn2 + 对桑椹色素色泽有一定的增色效果 ,但Fe3 + 则有明显的不良影响 ;食品中常用的葡萄糖、蔗糖、苯甲酸钠和山梨酸钾对桑椹色素无明显的不良影响 ;VC对桑椹色素有双重作用 ,而H2 O2 和Na2 SO3 对色素有严重的破坏作用。     

宿主域扩大的重组救活昆虫杆状病毒表达载体的构建及外源基因的表达

通过克隆的家蚕核多角体病毒解旋酶基因DNA与重组救活可线性化的苜蓿尺蠖核多角体病毒基因工程载体病毒 (BacPAK6 )DNA在昆虫细胞中发生重组 ,经累代筛选获得了既可感染家蚕又可感染秋粘虫细胞Sf 2 1的宿主域扩大的昆虫杆状病毒表达载体 (HyBacPAK6 )。HyBacPAK6DNA经Bsu36Ⅰ酶切后与含植酸酶基因的转移载体pVL1393 phy在家蚕细胞中重组后 ,通过蓝白斑筛选发现重组率可达 90 %以上。然而以杂交病毒为载体的外源基因表达量仅为以BmNPV为载体病毒的表达量的 2 0 %左右。分析认为HyBacPAK6可用于表达一些对蚕体有害的外源基因。     

中国狗牙根染色体数变异研究初报

通过国内30份狗牙根种源当色体数目的观测分析.结果表明,①我国狗牙根种质资源染色体数目呈现非常高的异质性,不同种源具有不同染色体数,同一种源不同根尖存在不同染色体数,同一根尖不同细胞亦具备不同染色本数,不同倍性平均出现的频率依次为:4n(32.26%)>n(18.98%)>3n(10.7%)>6n(2.13%)>2n(0.41%).此外,非整倍体平均比率高达32.1%.②染色体数目与纬度、经度、海拔间均无显著回归关系.该项研究为我国狗牙根种质资源开发利用提供了初步的试验依据.     

中华竹鼠驯化特性的研究

阐述了中华竹鼠的生活习性 ,并对其栖息地的构建 ,食物过渡 ,合群饲养等驯化特性进行了研究和分析 ,达到了便于人工饲养和繁殖的目的     

命案攻坚战警犬显神威

北京市公安局刑侦总队警犬侦查训练大队认真贯彻落实“全国侦破命案工作会议”精神,从抓警犬“出现场率”入手,充分发挥警犬技术在侦破命案专项斗争中的作用,并取得显著成效。     

遗传与环境在生物进化中的关系

本文讨论了在生物进化的过程中遗传与环境的关系及遗传物质的范围和生物可塑性的问题。遗传物质的变异是进化的内因，环境对遗传物质的变异起到诱发与筛选的作用，进化后的生物对环境又有反作用。     

河南省草种区划的研究

本文在前人研究的基础上 ,根据河南省自然条件的多样性和优质饲草生物学特点的差异性 ,提出了因地制宜地进行草种区划的具体方案 ,目的是为促进饲草的优质高产以及对环境条件的改良 ,切实解决当前畜牧业生产中优质饲草的不足和畜牧业生产环境日益恶化的局面。该草种区划的实施将有助于河南省 2 1世纪农牧业的多样性经营和可持续发展。     

硫辛酸对海马CA1区锥体细胞凋亡的抑制作用

采用股动脉放血并双侧颈总动脉夹闭制作全脑缺血再灌注模型 ,于术后 1 d处死动物 ,取脑 ,制作石蜡切片 ,通过 HE染色、Tunel检测 ,以观察海马 CA1区锥体细胞的损伤形式 ,并探讨α-硫辛酸 (α-lipoic acid,L A)对实验性脑缺血再灌注的神经保护作用。结果表明 :在短暂性全脑缺血中 ,海马锥体细胞存在着凋亡和坏死两种死亡形式 ;与对照组相比 ,缺血再灌注后1 d,海马 CA1区损伤较重 ,出现了较多的凋亡细胞 ,而 L A可明显抑制锥体细胞的凋亡。实验证明 :在脑缺血再灌注损伤中 ,细胞凋亡是神经元死亡的一种重要形式 ;L A对脑缺血再灌注有明显神经保护作用