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Six Friesian calves from a pedigree herd died or were killed within 1 week of birth because of progressive central nervous disease in which the only consistent lesion was cerebral oedema. The cause was citrullinaemia, resulting from an autosomally inherited dysfunction of the urea cycle enzyme arginosuccinate synthetase. Citrullinaemia was diagnosed by demonstrating markedly elevated concentrations of citrulline in the blood of one calf and in the cerebral spinal fluid of another. One of two sires used in the herd was a heterozygous carrier of the disease. Heterozygocity was demonstrated using a polymerase chain reaction/restriction endonuclease test designed to detect the genetic mutation that causes citrullinaemia in cattle.  相似文献   
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Twenty-eight research dogs were enrolled to determine the prevalence of salmonellae shedding after consumption of 1 Salmonella-contaminated commercial raw food diet meal. Sixteen dogs were exposed to Salmonella-contaminated commercial raw food diets and 12 to Salmonella-free commercial raw food diets. Seven of the exposed dogs shed salmonellae 1-7 days after consumption of Salmonella-contaminated raw food diets. None of the dogs fed Salmonella-free diets shed salmonellae. No clinical signs were observed in either group. Five of the 7 dogs shed the same serotypes as those recovered from food samples used for feeding. Results showed the same serotypes and antimicrobial resistance pattern in 2 of the 7 shedders. Dogs fed Salmonella-contaminated raw food diets can shed salmonellae and may, therefore, be a source of environmental contamination potentially leading to human or animal illness.  相似文献   
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Arable land in western Kenya is under considerable pressure from increasing human population. Rural households depend on farming for at least part of their livelihood, and poverty rates are among the highest in Kenya. Land is often depleted of nutrients, and for most farmers, access to inputs and markets is poor. There is a need to identify options that are manageable within the context of the farmer’s resource base and the household’s objectives that could improve farm household well-being. In this study we integrated qualitative informal participatory approaches with quantitative mathematical programming and biophysical simulation modelling. Households in four sub-locations in Vihiga District were clustered and pilot cases identified. Meetings were held with farmers to elicit their perceptions of what their ideal farm would look like, and how its performance might compare with their own farm’s performance. With farmers’ help, a range of scenarios was analysed, relating to changes in current enterprise mixes, changes in current farm sizes, and changes in prices of staples foods and cash crops. A considerable mismatch was found between farmers’ estimates of their own farm’s performance, and what was actually produced. There seems to be a threshold in farm size of 0.4 ha, below which it is very difficult for households to satisfy their income and food security objectives. Even for larger farms whose households are largely dependent on agriculture, the importance of a cash crop in the system is critical. There is a crucial role for extension services in making farmers aware of the potential impacts on farm revenue of modest changes in their farm management systems. We are monitoring nine households in the district, whose farmers have made some changes to their system in an attempt to increase household income and enhance food security.  相似文献   
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Food quality aspects of farmed turbot (Psetta maxima) were compared following two methods of slaughter: the current commercial method, by immersion in an ice slurry, which is then dewatered after approximately 20 min, or by first humanely, electrically stunning the fish using a prototype commercial stunner, before immersion in an ice slurry, which is dewatered after 20 min. Quality was assessed for up to 10 days of storage on ice following slaughter. No differences were found between the slaughter methods in terms of an overall carcass quality: overall appearance, haemorrhage, damage, burst gall bladder, staining of the body cavity by leakage from the gut or damage to the spine. No detectable difference was found between the treatments using the industry standard freshness scoring system, the Quality Index Method. Both groups of fish were classified as ‘Fresh’ after 10 days of storage on ice. Using objective measurements of colour, no differences between fish from either treatment were found in fillet colour. Changes in flesh pH were similar in electrically stunned and traditionally killed fish with a mean pH (±SE) at 2 h post‐mortem of 6.80±0.027 declining to 6.44±0.032 at 24 h post‐mortem. Humane electrical stunning of turbot at slaughter neither detectably improved nor decreased product quality as measured between 1 and 10 days of storage on ice.  相似文献   
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Weather uncertainty and soil spatial variability impact nitrogen (N) cycling and corn (Zea mays L.) growth, making accurate N predictions a challenge. Field studies were conducted in Lansing, Michigan, to evaluate a computer model (i.e., Adapt-N), a preseason year-based model (i.e., maximum return to N [MRTN]), and a crop sensor model (i.e., active canopy sensor with algorithm) for recommending corn N rates. To determine site-specific economic optimum N rates (EONR), five N rates were also applied (0, 33%, 66%, 133%, and 166% of the suggested MRTN) as starter + sidedress (SD) at V4. In a wet year (i.e., 2015), Adapt-N increased V8 SD N rates 35 kg N ha?1 relative to the MRTN V4 SD N application. Although the greater rate of N may have provided additional yield protection, no statistical yield differences were observed between the two models. The MRTN model increased partial factor productivity (PFP) 20% relative to Adapt-N. Limited expression of V8 corn N deficiency reduced crop sensor total N rates (21–56 kg N ha?1) and yield (0.82–1.05 Mg ha?1) relative to other models. In a drier year (i.e., 2016), N demand was reduced (EONR 64 kg N ha?1 less than 2015), resulting in similar corn response to all three models. Despite differences in actual corn N rate recommendations, all three models resulted in similar economic net returns across study years.

Abbreviations: EONR, economic optimum nitrogen rate; MRTN, Maximum Return to Nitrogen; NUE, nitrogen-use efficiency; PFP, partial factor productivity; SBNRC, sensor-based nitrogen rate calculator; SD, side-dress  相似文献   
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