Microbial immobilisation and turnover of N labelled substrates in two arable soils under field and laboratory conditions

Microbial biomass N dynamics were studied under field and laboratory conditions in soils of high yield (HY) and low yield (LY) areas in an agricultural field. The objective of the study was to determine the size and activity of soil microbial biomass in the soils of the different yield areas and to compare these data obtained under field and laboratory conditions. Soils were amended with ¹⁵N labelled mustard (Sinapis alba) residues (both experiments) and labelled nitrate (laboratory only) at 30 μg N g⁻¹ dry soil. Soil microbial biomass (SMB) N, mineral N (N_min) and total N content was monitored both in the field and in the laboratory. N₂O efflux was additionally measured in laboratory treatments. Isotope ratios were determined for SMB in both experiments, for all other parameters only in the laboratory treatments. In the laboratory less amounts of added substrate N were immobilised by the SMB in HY soils compared to LY soils, whereas in the field immobilisation of added N by SMB was higher in HY soils initially and slightly lower after 40 days of incubation. Calculated turnover times in the laboratory nitrate, laboratory mustard and field mustard amendments were 0.18, 0.27 and 0.74 years (HY) and 0.22, 0.61 and 1.01 years (LY), respectively. The turnover times of added substrate N always showed the trend to be faster in HY soils compared to LY soils. A faster turnover of nutrients in the HY soils may involve a better nutrient supply of the plants, which coincides with the higher agricultural yield observed in these areas.     

Enzymatic synthesis of aroma compound xylosides using transfer reaction by Trichoderma longibrachiatum xylanase

Enzymatic synthesis of aroma compound xylosides was performed by Trichoderma longibrachiatum xylanase. Information concerning the nature of xylosides present in the reaction medium was obtained by GC-EI-MS, by GC-NCI-MS of TFA derivatives, and by positive FAB-MS of the reaction mixtures. Moreover, the structures of isolated benzyl beta-D-xylopyranoside and 4-O-beta-xylopyranosyl-beta-D-xylopyranoside were established by (1)H and (13)C NMR and heteronuclear two-dimensional ((1)H-(13)C) chemical shift correlation. The results obtained for hexyl and benzyl alcohol xylosides indicated that a reaction implying a transfer of one to two or three xylose units from xylan was involved. The enzyme was able to recognize xylobiose, xylotriose, and xylan as xylose donors. Benzyl xyloside, produced independently of xylobioside and xylotrioside, was found as the major kinetic product of the reaction. Benzyl xyloside was produced in higher quantities and at a higher rate than that obtained for the di- and trixyloside derivatives. The maximum production for benzyl xyloside, 1.29 g/L, was obtained in the presence of hexane (50%) used as cosolvent. Xylosides and xylobiosides of several aroma compounds, (Z)-hex-3-en-1-ol, heptan-2-ol, geraniol, nerol, and citronellol, were synthesized in different amounts, from 850 mg/L for (Z)-hex-3-en-1-yl xylosides to 1.5 mg/L for citronellyl xylosides. No synthesis occurred when menthol, linalool, and eugenol were used as acceptors.     

Laccase and phosphatase activities of the dominant ectomycorrhizal types in a lowland oak forest

The year-round dynamics of laccase and acid phosphatase activities of ectomycorrhizas (EM) were monitored in an oak forest, with a microplate photometric method using individual EM root tips. Among the most frequent and abundant EM morphotypes, those of Lactarius quietus and Cortinarius anomalus showed a peak of laccase activity in spring, while those of Xerocomus chrysenteron displayed highest laccase activities in summer and autumn. In comparison, less seasonal differences were observed in EM acid phosphatase activity. This suggests that the different EM types within the EM community perform complementary functions depending on season.     

Agricultural Sediment Reduction by Giant Cane and Forest Riparian Buffers

The sediment filtering capabilities of giant cane (Arundinaria gigantea (Walt.) Chapm.) and forest riparian buffers were compared in a southern Illinois, USA non tile drained agricultural watershed. Giant cane, a bamboo species, serves as important wildlife habitat throughout its native range in the southeastern and lower midwestern United States. Overland flow samples were collected at the field edge and at 3.3 m, 6.6 m, and 10.0 m within the riparian buffers during 19 precipitation events over a 1-year period. On an annual basis, significant sediment reductions occurred by 3.3 m and 6.6 m in the cane and forest buffers, respectively. The giant cane buffer reduced incoming sediment mass by 94% within the first 3.3 m, while the forest buffer reduced sediment by 86% over 6.6 m. Within 10.0 m of the field edge, the cane and forest buffers reduced sediment mass by 100% and 76%, respectively. On a seasonal basis, the cane buffer outperformed the forest buffer. During each of the four seasons, the cane buffer reduced sediment masses within 3.3 m of the field edge, while the forest buffer showed initial reductions occurring at 6.6 m during the summer, fall, and winter. No detectable reductions occurred during the spring in the forested buffer. Reductions in sediment concentrations were less evident compared to mass basis, indicating that infiltration may be a more important sediment reduction mechanism than particle settling. Both the forest and giant cane buffers had relatively high measured soil infiltration rates. Study results indicate that giant cane is an appropriate species to include in riparian buffer restoration designs for sediment control.     

Making modern agriculture sustainable: Fam research network on agroecosystems

All forms of agriculture cause changes in the balances and fluxes of preexisting ecosystems, thereby limiting self-regulatory ecosystem (resiliency) functions. The intensive agriculture of the past, with its strong reduction of landscape structures and vast decoupling of energy and matter cycles, has caused stress and degradation of the production base; massive influence has also been exerted on neighboring compartments. This has resulted in the well known problems of pesticide loads, high phosphate loads to surface waters via over-fertilized soils or erosion as such. To overcome the economic, social and political inadequacies leading to ecological degradation, the demand for sustainable agricultural management needs to be transposed into knowledge-based practical instructions and political regulations on a regional scale. Thus, applied research for a sustainable and ecologically compatible land use aimed at sufficient food production is ever so important. In the FAM, thirty German research institutes have merged to perform research on this topic.