枣树病虫害防治智能决策支持系统双向推理机的设计

研究了枣树病虫害防治智能决策支持系统的推理机制及搜索策略,并在WINDOWS2000下采用vc++6.0的面向对象的程序设计语言构建了枣树病虫害防治智能决策支持系统的双向推理机,经过反复测试,枣树病虫害防治智能决策支持系统运行良好,可以及时准确的对枣树的病虫害进行预测、诊断并防治。     

油菜浙双6号栽培技术试验

油菜浙双6号是浙江省农科院作核所选育的双低油菜新品种.栽培试验结果表明,在建德市适宜9月15日左右播种,10月20日移栽,种植密度11.25万株/hm2,折施纯氮210 kg/hm2.早播、肥料足,不仅秧苗素质好,而且1次分枝多、角果数、粒数多,有利于高产稳产.     

菜地土壤N_2O排放及其氮素反硝化损失

采用培养试验方法,对南京郊区 3对菜地土和水稻土的 N2O排放和氮素反硝化损失进行了研究.不加乙炔培养测定土壤 N2O排放,加乙炔( 10% V/V)培养测定反硝化损失.菜地土为相同类型水稻土改种为蔬菜约 20年的土壤.结果表明,在培养 0～ 1 d期间 ,菜地土本身 N2O排放通量 (5.15～ 218.37 ng N· g-1soil· h-1)均高于相同类型的水稻土 (2.50～ 3.94 ng N· g-1soil· h-1). 3对供试土壤中, 2个菜地土培养 21 d排放的 N2O总量与反硝化损失总量均显著高于相同类型的水稻土( P<0.05). 3对供试土壤施尿素后反硝化损失均未显著增加.施肥和不施肥处理,土壤 N2O排放累积量和反硝化损失累积量随时间 t的变化均符合修正的 Elovich方程 y=bln(t)+ a.     

采用ASPCR技术检测抗药性雨久花ALS突变基因 碱基种类的探讨

等位基因特异性扩增(Allele-specific PCR简称:ASPCR)技术是一项快速、简便、低成本的检测等位基因单核苷酸多态性的新方法。作者针对雨久花感抗磺酰脲类生态型ALS基因编码区第592位碱基密码(ALS氨基酸第197位)特征,采用在引物3′末端设有胸腺嘧啶核苷酸(感性)或鸟嘌呤(抗性)核苷酸的2种引物,对延边稻田发生的雨久花感抗磺酰脲类生态型ALS基因编码区第592位进行了ASPCR。检测结果显示,当以引物3′末端设有胸腺嘧啶引物时,感性类型出现ASPCR扩增带,而抗性类型无带出现,反之抗性类型有带而感性类型无带,检测到延边稻田发生的抗磺酰脲类雨久花生态型的ALS 197位点的脯氨酸被组氨酸替代,证明了该方法的有效性和可靠性。     

线粒体中活性氧的产生途径和测定方法

活性氧(ROS)是自然界普遍存在的含氧化合物的总称,对生物体的生理和细胞稳态的调节具有重要意义.但活性氧的测定方法仍然是生物研究领域的一个难点,本文综述了线粒体中活性氧的产生途径和测定方法.     

围栏封育下山地灌草丛草地植被植物多样性与生物量的研究

 2006年3月，以云南省永胜县内干热河谷地区的退化草地为研究对象，在2004年设置的围栏封育区和对照区内，对植物的盖度、密度、频度、高度以、地上生物量及植物物种多样性进行了调查和研究。结果表明，围栏封育后退化草地的植被盖度明显变化，盖度总体变化趋势为：轻度退化围栏封育区>轻度退化对照区>重度退化围栏封育区>重度退化对照区。群落多样性总体表现同盖度的表现一样。群落均匀度指数、丰富度基本上与群落多样性指数呈正相关。群落的生态优势度指数与群落多样性指数呈负相关。围栏封育区地上生物量均高于对照，生态和经济效益明显。随着退化程度加剧，分布在各层的植物根量越来越少，地下根系具有浅层化特点。     

实现教育信息化,将我院建设成为具有鲜明特色的现代化的沿海都市型高等农业院校--简述我院"网络与现代教育技术中心"建设的几个问题

从若干方面探讨了我院网络与现代教育技术中心的建设,其要点是:我院现代教育技术中心的职能;校园网和多媒体教室是极为重要的基础教学设施;教育信息化的内涵;e-learning和混合学习(blended learning)模式;建设数字校园;利用网络资源是一个教师必须具备的基本能力;要把网上教学资源的开发与利用放在首位;尝试开展农业科学技术远程教育.     

奶牛催乳素基因多态性与产奶性状的关系

通过PCR-RFLP的方法,分析催乳素基因exon3在543头北京荷斯坦母牛中的多态性,得到3种基因型:AA、AB和BB,基因型频率分别为0.734,0.257,0.009,并对不同基因型间产奶性能进行了最小二乘法拟合线性模型分析。结果表明:在泌乳Ⅰ期,AA和AB基因型奶牛的奶、乳脂和乳蛋白产量高于BB基因型(P<0.01),在泌乳Ⅱ期,AB基因型奶牛的的奶、乳脂和乳蛋白产量高于AA基因型(P<0.01);在泌乳Ⅱ和Ⅲ期,AA基因型奶牛的乳蛋白率高于AB基因型(P<0.05)。     

Genetic polymorphism of caspase-7 isoform β in patients with rheumatoid arthritis

AIM: To investigate the association between the rs2227309 polymorphism of cysteinyl aspartate-specific protease-7 (caspase-7) isoform β and the genetic susceptibility in rheumatoid arthritis (RA) patients in Taizhou of China. METHODS: Genotyping of rs2227309 of caspase-7 isoform β gene was performed in 204 RA patients and 203 matched healthy controls using TaqMan single nucleotide polymorphism (SNP) genotyping assays. RESULTS: The genotype frequencies of GG, AG and AA of caspase-7 polymorphism in the RA patients were 33.3%, 53.4% and 13.2%, respectively, and 33.0%, 44.3% and 22.7% in the healthy individuals,respectively. There was a significant difference in caspase-7 genotype frequencies between the RA patients and healthy controls (P<0.05). The frequency of GG+AG genotype in RA patients was higher than that in healthy controls with significant difference (P<0.05, OR=1.921, 95%CI: 1.140~3.236). The frequencies of the G allele were 60.0% and 55.2% in the RA patients and the healthy individuals,respectively. No significant difference was observed in allele frequency between the RA patients and healthy controls (P>0.05, OR=1.221, 95%CI: 0.924~1.613). CONCLUSION: The rs2227309 polymorphism of caspase-7 isoform β gene is associated with the susceptibility to rheumatoid arthritis. The high production of the non-functional variant of caspase-7 may reduce the apoptosis of rheumatoid synovial cells, indicating the mechanism of this association.     

Changes of neuropeptides and electrolytes in patients with acute traumatic brain injury

AIM: To investigate the levels of neuropeptides and electrolytes in the patients with acute traumatic brain injury.METHODS: Seventy-eight patients with acute brain injury were divided into mild, moderate and severe groups according to their GCS scores. The serum levels of arginine vasopressin (AVP) and angiotensin II (Ang II) were measured on day 1, 3 and 7 after injury. The serum levels of electrolytes were also measured on day 1. Forty-one subjects who received healthy check-up served as normalcontrols. RESULTS: Compared with normal control group, the serum levels of AVP and Ang II significantly increased in the patients with traumatic brain injury (P<0.01), depending on the severity of brain injury. Both neuropeptides reached the peak on day 3 after injury. The concentrations of serum potassium and calcium decreased in the patients with acute brain injury(P<0.01),also showing a severity-dependent tendency. No significant change of serum sodium in the patients with brain injury was observed. CONCLUSION: The serum levels of arginine vasopressin and angiotensin II canalso be used as the severity indicators of traumatic brain injuries. Decrease in serum potassium and calcium can also be used to evaluate the severity in patients with acute traumatic brain injury.