The Genetic Structure of Australian Populations of Mycosphaerella musicola Suggests Restricted Gene Flow at the Continental Scale

ABSTRACT Mycosphaerella musicola causes Sigatoka disease of banana and is endemic to Australia. The population genetic structure of M. musicola in Australia was examined by applying single-copy restriction fragment length polymorphism probes to hierarchically sampled populations collected along the Australian east coast. The 363 isolates studied were from 16 plantations at 12 sites in four different regions, and comprised 11 populations. These populations displayed moderate levels of gene diversity (H = 0.142 to 0.369) and similar levels of genotypic richness and evenness. Populations were dominated by unique genotypes, but isolates sharing the same genotype (putative clones) were detected. Genotype distribution was highly localized within each population, and the majority of putative clones were detected for isolates sampled from different sporodochia in the same lesion or different lesions on a plant. Multilocus gametic disequilibrium tests provided further evidence of a degree of clonality within the populations at the plant scale. A complex pattern of population differentiation was detected for M. musicola in Australia. Populations sampled from plantations outside the two major production areas were genetically very different to all other populations. Differentiation was much lower between populations of the two major production areas, despite their geographic separation of over 1,000 km. These results suggest low gene flow at the continental scale due to limited spore dispersal and the movement of infected plant material.     

Lack of biocontrol capacity in a non-pathogenic mutant of <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> f. sp. <Emphasis Type="Italic">melonis</Emphasis>

The aim of this study was to assess the biocontrol capacity of rev157, a non-pathogenic mutant of a pathogenic strain of Fusarium oxysporum f. sp. melonis (Fom24). Inoculated in association with the virulent parental strain, the mutant rev157 did not protect the host plant (muskmelon) against infection by Fom24. Applied on flax, a non-host plant, the mutant rev157 was not able to protect it against its specific pathogen F. oxysporum f. sp. lini. On the contrary the parental strain Fom24 did protect flax as well as a soil-borne biocontrol strain (Fo47). Since the mutant rev157 was affected neither in its growth in vitro nor in its capacity to penetrate into the roots, it can be speculated that the mutation has affected traits responsible for interactions within the plant. In F. oxysporum the pair of strains Fom24/rev157 is a good candidate to identify genes involved in the biocontrol capacity of F. oxysporum and to test the hypothesis of a link between capacity to induce the disease and capacity to induce resistance in the plant.     

An inter-laboratory comparison of multi-enzyme and multiple substrate-induced respiration assays to assess method consistency in soil monitoring

The use of indicators in soil monitoring schemes to detect changes in soil quality is receiving increased attention, particularly the application of soil biological methods. However, to date, the ability to compare information from different laboratories applying soil microbiological techniques in broad-scale monitoring has rarely been taken into account. This study aimed to assess the consistency and repeatability of two techniques that are being evaluated for use as microbiological indicators of soil quality: multi-enzyme activity assay and multiple substrate-induced respiration (MSIR). Data were tested for intrinsic (within-assay plate) variation, inter-laboratory repeatability (geometric mean regression and correlation coefficient) and land-use discrimination (principal components analysis). Intrinsic variation was large for both assays suggesting that high replicate numbers are required. Inter-laboratory repeatability showed diverging patterns for the enzyme assay and MSIR. Discrimination of soils was significant for both techniques with relatively consistent patterns; however, combined laboratory discrimination analyses for each technique showed inconsistent correspondence between the laboratories. These issues could be addressed through the adoption of reliable analytical standards for biological methods along with adequate replication. However, until the former is addressed, dispersed analyses are not currently advisable for monitoring schemes.     

一些优质小麦及其杂种后代高分子量谷蛋白亚基组成与面包品质之关系

应用ＳＤＳ－ＰＡＧＥ技术分析了陕优２２５等１６个品种及３个组合杂种后代分离材料高分子量谷蛋白亚基组成及与面包品质的关系。结果表明，普通小麦中罕见的Ｇｌｕ－Ｂ１编码来基１４＋１５象Ｇｌｕ－Ｄ１编码亚基５＋１０一样，对面包品质有重要贡献，存在于陕优２２５、小偃６号等优质品种中。     

Comparison and sensitivity of measurement techniques for spatial distribution of soil salinity

In Khorezm, a district of Uzbekistan situated in the Aral Sea Basin, soil salinization is an important driver of soil degradation in irrigated agriculture. The main objective of this study was to identify techniques that enable rapid estimation of soil salinity. Therefore, bulk electrical conductivity of the soil (EC_a-meas) was measured with three different devices (2P, 4P, and CM-138) and electrical conductivity of the soil paste (EC_p-meas) was measured with the so-called 2XP device. These measurements were compared with independent estimates of EC_a-calc and EC_p-calc based on laboratory measurements of the saturated extract, EC_e, of soil samples from the same sites. Soil salinity could be assessed satisfactorily with all four devices. EC_p-meas could be well reproduced by the 2XP device (R ² = 0.76), whereas EC_a-meas estimates using 2P, 4P, and CM-138 in the field were less accurate (R ² < 0.50). The sensitivity of all devices to the main ions Cl⁻ and Ca^{2 +} suggests that the measuring principles are similar for all instruments. The devices can therefore be used interchangeably. Field assessment of soil salinity was considerably enhanced by the use of CM-138, because large areas can be quickly assessed, which may be desirable in spite of the lower accuracy.     

Interpreting Soil Electrical Conductivity and Terrain Attribute Variability with Soil Surveys

Utilizing soil electrical conductivity (EC) measurements and terrain attributes for precision management will require secondary soil information for adequate interpretation. The objective of this study was to determine whether readily available second-order soil surveys were of adequate quality to aid with interpreting soil EC and terrain data. For three locations in Kentucky, USA, first-order soil surveys were created, second-order surveys reports were obtained, elevation was measured and used to calculate terrain attributes (slope, aspect, plan curvature, profile curvature), and bulk soil electrical conductivity was measured. Three analytical methods (an ordinary least squares analysis and two random field analyses), visual map assessment, and examination of least-squares means were used to assess the relationships between soil EC measurements, terrain attributes and first- and second-order soil surveys. The OLS and random field analyses were problematic. However, the ranking of the OLS F-statistics appeared to reflect the general relationship between landscape variables and first-order soil surveys. The landscape variables related particularly well with soil properties that had been impacted by past soil erosion. Unfortunately, however, second-order soil surveys in this study were not created at suitable scales to adequately interpret EC and terrain data regarding erosion history or other attributes. While these surveys may provide some useful information, field measurements, sampling, and observations will likely be required to develop high quality interpretations of soil EC and terrain attribute data.     

Nesting ground abandonment by the maleo (Macrocephalon maleo) in North Sulawesi: Identifying conservation priorities for Indonesia’s endemic megapode

The maleo (Macrocephalon maleo) is an endemic bird of Sulawesi that lays eggs in communal nesting grounds incubated by solar or geothermal sources. In recent years, maleo nesting grounds have become increasingly vulnerable to threats associated with the growing human population of the island. Consequently, the maleo has become one of the most imperiled bird species in Indonesia and is classified as Endangered on the IUCN Red List. We report on the 2004 status of maleo nesting grounds in North Sulawesi focusing on the Bogani Landscape, the region in and around Bogani Nani Wartabone National Park, where the nesting grounds are concentrated. We compare our results to those of a similar study in 1990-1991, examining changes in nesting ground status in relation to habitat and land use factors such as protection status, geographic position (coastal or inland), and distance to forest, human settlements, and roads. Nesting grounds were more likely to be abandoned if they were: (a) in coastal areas; (b) near settlements and roads; and (c) not in protected areas. Binary logistic regression identified connectivity between nesting grounds and forest in 1991 as the best predictor of whether or not sites were abandoned by maleo over the next 12 years; a loss of local continuity between nesting grounds and forest is associated with a sixfold increase in the probability of abandonment. Access to forest was itself highly correlated with other variables examined, and thus represented a composite index of threats to maleo. The application of this model to the 2004 data identified six active nesting grounds that are at high risk of abandonment in the near future, seven at intermediate risk, and five at low risk. These results offer conservationists priorities for future maleo conservation.     

A lysimeter study of the fate of fertilizer nitrogen in spring barley crops grown on a shallow soil overlying Chalk: denitrification losses and the nitrogen balance

Four successive spring barley crops were grown in monoliths of a shallow soil overlying Chalk, contained in lysimeters. After harvest of the fourth crop, 25% of the nitrogen-15 labelled fertilizer applied 4 years earlier was found remaining in the roots and soil. Of this, 73% was present in the upper 30cm of the profile. From the amounts of fertilizer derived nitrogen that remained at the beginning of each cropping season we estimate that 5–6% of the residual nitrogen-15 turned over each year, representing a net release of 20% of the labelled nitrogen contained in the microbial biomass. Mineralization of the total biomass at the same fractional rate would release 120 kg N ha^?1 a^?1. This estimate is supported by the difference between input and outputs of total nitrogen during the experiment of 76–94 kg N ha^?1 a^?1 in fertilized lysimeters and 129kg N ha^?1 a^?1 in unfertilized control lysimeters. The total recovery of the applied labelled nitrogen was 81–87%. The nitrogen not accounted for was taken to be lost by denitrification of nitrate to dinitrogen, as no nitrous oxide emissions were detected during the experiment. Laboratory studies in aerobic and anaerobic conditions in presence of acetylene confirmed that 10–20% of the applied nitrogen-15 could have been transformed to dinitrogen.