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排序方式: 共有184条查询结果,搜索用时 15 毫秒
71.
Nobuya Tashiro Mayumi Noguchi Youichi Ide Fumitaka Kuchiki 《Journal of General Plant Pathology》2013,79(2):158-161
In June 2006, a large number of small black spots containing a branching network of black hyphae developed on shipped Satsuma mandarin fruits grown in greenhouses, which markedly decreased their commercial value. The fungus that was frequently isolated from the damaged fruits was identified as Cladosporium cladosporioides. Inoculation of fruits with the fungus caused the same symptoms, and the fungus was reisolated from the diseased fruits. The disease caused by this fungus has not been previously documented in citrus fruits; we name this new disease “sooty spot” on the basis of its symptoms. 相似文献
72.
Oshima M Fujimura M Banno S Hashimoto C Motoyama T Ichiishi A Yamaguchi I 《Phytopathology》2002,92(1):75-80
ABSTRACT Partial DNA fragments of Botrytis cinerea field isolates encoding the putative osmosensor histidine kinase gene (BcOS1) were cloned by polymerase chain reaction amplification and the predicted amino acid sequences were compared between dicarboximide-sensitive and resistant field isolates. The predicted BcOS1p is highly homologous to osmosensor histidine kinase OS1p from Neurospora crassa including the N-terminal six tandem repeats of approximately 90 amino acids. Four dicarboximide-resistant isolates of B. cinerea (Bc-19, Bc-45, Bc-682, and Bc-RKR) contained a single base pair mutation in their BcOS1 gene that resulted in an amino acid substitution in the predicted protein. In these resistant isolates, codon 86 of the second repeat, which encodes an isoleucine residue in sensitive strains, was converted to a codon for serine. The mutation of Botrytis field resistant isolates was located on the second unit of tandem amino acid repeats of BcOS1p, whereas the point mutations of the fifth repeat of OS1p confer resistance to both dicarboximides and phenylpyrroles and also osmotic sensitivity in Neurospora crassa. These results suggest that an amino acid substitution within the second repeat of BcOS1p is responsible for phenotypes of field resistant isolates (resistant to dicarboximides but sensitive to phenylpyrroles, and normal osmotic sensitivity) in B. cinerea. 相似文献
73.
In March 2006, stored fruits of the medium-to-late-ripening citrus variety Shiranuhi ([Citrus unshiu × C. sinensis] × C. reticulata) were found to have a disease similar to blue mold. The fungus causing this disease differed distinctly from the well-known,
blue mold agent, Penicillium italicum, because it formed whisker-like coremia measuring 1–8 mm. On the basis of the morphological characteristics and the phylogenetic
analysis based on the nucleotide sequence of the β-tubulin gene, the fungus was identified as P. ulaiense. This is the first report of citrus whisker mold caused by P. ulaiense in Japan. 相似文献
74.
Kuwamura M Ide M Yamate J Shiraishi Y Kotani T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(10):1117-1119
A 4-year-old male Shiba dog initially presented with pain of an undetermined origin and hypersensitivity to touch. Seven days later, the dog developed ataxia, hind-leg weakness and knuckling. The dog died on 20 days after presentation. Postmortem examination revealed a mass in the body of thoracic vertebra. Histopathologically, the mass consisted of granulomatous inflammation, including fungal organisms that were immunohistochemically positive for Candida albicans. Similar granulomatous lesions were observed in the systemic lymph nodes, kidneys, pancreas, spleen, prostate gland, thyroid glands and heart. This case was diagnosed as systemic candidiasis with spondylitis. 相似文献
75.
Masato?Kawabe Kohei?Mizutani Takanobu?Yoshida Tohru?Teraoka Katsuyoshi?Yoneyama Isamu?Yamaguchi Tsutomu?ArieEmail author 《Journal of General Plant Pathology》2004,70(1):16-20
We selected a reduced-pathogenicity mutant of Fusarium oxysporum f. sp. lycopersici, a tomato wilt pathogen, from the transformants generated by restriction enzyme-mediated integration (REMI) transformation. The gene tagged with the plasmid in the mutant was predicted to encode a protein of 321 amino acids and was designated FPD1. Homology search showed its partial similarity to a chloride conductance regulatory protein of Xenopus, suggesting that FPD1 is a transmembrane protein. Although the function of FPD1 has not been identified, it does participate in the pathogenicity of F. oxysporum f. sp. lycopersici because FPD1-deficient mutants reproduced the reduced pathogenicity on tomato.The nucleotide sequence data reported are available in the DDBJ/EMBL/GenBank databases under the accession number AB110097 相似文献
76.
77.
To investigate the factors governing retention of pesticidal spray solutions on plant surfaces, a new retention model for droplets on solid surfaces has been introduced as where ΔΘ = Θa – Θr. The minimum sliding volumes (MiSV) of surfactant aqueous solutions (2 g litre?-1; surface tensions; 28·5 – 72·5 mN m?-1) on smooth surfaces of paraffin wax and polymer sheets were measured and the validity of the above retention equation was verified on combinations of these solutions and surfaces. A concept of the specific adhesional force (Fa) of a droplet was defined as which was the maximum adhesional force on a unit length of the width of the contact circle of a droplet, and Fa values of the solutions on the surfaces were compared. The predominant factors for droplet retention are discussed on the basis of this model. 相似文献
78.
79.
80.
Detection of antibodies against Akabane virus in bovine sera by enzyme-linked immunosorbent assay 总被引:5,自引:0,他引:5
S Ide K Baba M Tsuchimoto H Nagano Y Eiguchi T Yamagami H Yamagishi Y Tanaka Y Fujisaki T Hohdatsu 《Veterinary microbiology》1989,20(3):275-280
An enzyme-linked immunonosorbent assay was established for detection of antibodies to Akabane virus in bovine sera. The assay was shown to be a useful serological tool for studies on Akabane virus infection. 相似文献