羊种布鲁氏菌疫苗株PCR-RFLP鉴定方法的建立

为建立一种羊种布鲁氏菌Rev.1疫苗株PCR-RFLP鉴定方法,利用羊种布鲁氏菌Rev.1具有链霉素抗性的特性,选取与链霉素抗性相关编码核糖体蛋白S12的rps L基因为模板设计引物,经PCR扩增后,将产物进行Nci I酶切,发现羊种布鲁氏菌疫苗株Rev.1出现约500 bp条带,而不具有链霉素抗性的羊种布鲁氏菌株16M则出现384 bp条带。结果表明,PCR-RFLP方法能够用于羊种布鲁氏菌Rev.1疫苗株的鉴定,这为今后区分羊种布鲁氏菌疫苗株Rev.1免疫与野株感染提供了基础。     

2015―2020年国家西北内陆棉区棉花品种区域试验参试品种(系)枯黄萎病抗性评述

对2015-2020年参加西北内陆棉区区域试验的192个棉花品种(系)枯萎病、黄萎病的抗性分析,为棉花抗病育种及品种推广提供依据.结果表明,参试棉花品种(系)未出现对枯萎病和黄萎病免疫的品种;参试棉花品种(系)对枯萎病的抗性水平较高,除2018年高抗和抗病品种(系)占比为88.2％外,其他年份均在92％以上;参试的品种...     

遵义市辣椒产业发展的现状、问题与对策

本文概述了遵义市辣椒产业发展的基本情况及存在的问题,进一步提出了当前遵义辣椒产业发展的思路与措施。     

基本建设单位对工程建设项目的管理程序

工程建设项目的管理程序是基本建设单位进行项目管理的关键.本文从项目的前期准备、工程项目建设、工程项目验收三个阶段论述了基本建设单位对工程建设项目的管理程序.     

森工劳动保障计算机网络需求分析

本文针对劳动和社会保障的具体义务,对森工劳动和社会保障计算机网络进行了分析.     

粮仓智能通风控制系统

综合了当今先进的仓储管理技术、电子技术、计算机网络技术、人工智能,通讯技术及防腐防雷技术为一体,设计一套粮仓智能通风控制系统,以实现在外部条件适宜的情况下对粮仓进行通风,达到除热、调湿等目的,进而有效控制粮仓的储粮环境,从而达到安全储粮的目的.     

Concentration variations of several ions in stream after a wildfire

In May 2006, a high intensity wildfire occurred in Songling forest region in Daxing＇an Mountains, China. The concentration changes of eight ions （K^＋, Na^＋, Ca^2＋, Mg^2＋, Cl^-, Br^-, NO3^- and SO4^2-） were measured in burned and tmbumed streams after fire from May to Oct., 2006. Results show that the most ions flux were higher in burned stream than that in unburned stream during the sampling period, and the greatest concentrations of most ions transported from burned stream occurred in July. After fire, the most amplitude chemical ion was Ca^2＋, whose average concentration was 5.50 mg·L^-1 higher than that in unburned stream, and the total concentration of every chemical ion presents a trend Ca^2＋〉SO4^2- 〉Na^＋〉Mg^2＋〉NO3^- . The average concentrations of Ca^2＋, SO4^2- , Na^＋＇Mg^2＋,NO3^- showed an increase trend, but those of K^＋, Cl^- , Br had a decreased trend. SO4^2- had the largest loss among these anions, followed by NO3^-. Overall, the increase degree of cation was greater than that of anion after burning.     

60多年来车尔臣河流域主要水文要素特征分析

开展河流主要水文要素变化特征分析,可为不同来水情景下水资源配置（农业需水量计算、山区水库调蓄计算、水利工程规划布局、水资源优化利用等）提供理论依据。以新疆车尔臣河为例,采用Mann-Kendall趋势检验、小波分析等方法对主要水文要素变化特征进行了分析。车尔臣河径流系列变差系数（Cv）为0.34,偏差系数（Cs）为1.14,径流年际变化相对稳定;1957—2021年间径流整体呈显著增加趋势,其中1957—2003年处于上升、下降的波动状态,在2003年后径流整体呈增加趋势,且在2008年发生突变;径流过程存在 11 a和 19 a的显著主周期,在主周期内还存在 7 a和 11 a的小周期;基于距平百分率法得到丰水年来水为6.630亿m³,平水年来水量为5.319亿m³,枯水年来水量为4.820亿m³;流域降水呈现显著上升趋势,气温呈先下降后上升波动,整体呈上升趋势。研究表明,1957—2021年,在车尔臣河流域,径流年际变化相对稳定,整体呈显著增加趋势,过程存在11 a和19 a的显著主周期,降水呈波动上升趋势,气温也呈增加趋势,在未来水资源配置规划时应重点关注以上特征。     

Approaches to Configuration Determinations of Flexible Marine Natural Products: Advances and Prospects

Flexible marine natural products (MNPs), such as eribulin and bryostatin, play an important role in the development of modern marine drugs. However, due to the multiple chiral centers and geometrical uncertainty of flexible systems, configuration determinations of flexible MNPs face great challenges, which, in turn, have led to obstacles in druggability research. To resolve this issue, the comprehensive use of multiple methods is necessary. Additionally, configuration assignment methods, such as X-ray single-crystal diffraction (crystalline derivatives, crystallization chaperones, and crystalline sponges), NMR-based methods (JBCA and Mosher’s method), circular dichroism-based methods (ECCD and ICD), quantum computational chemistry-based methods (NMR calculations, ECD calculations, and VCD calculations), and chemical transformation-based methods should be summarized. This paper reviews the basic principles, characteristics, and applicability of the methods mentioned above as well as application examples to broaden the research and applications of these methods and to provide a reference for the configuration determinations of flexible MNPs.     

野生动物在人兽共患病发生、传播中的作用

近年来,人兽共患病已经成为影响全球的重大公共卫生问题,野生动物作为许多人兽共患病原体的"储存宿主",其作用正为越来越多的人所重视.介绍几种古老的和近年来新出现的重大人兽共患病的流行和危害情况,阐述野生动物在其发生和流行中的作用,探讨人兽共患病的预防、控制措施.